Supramolecular structure of the salmonella typhimurium type III protein secretion system

Science

Volumn 280, Issue 5363, 1998, Pages 602-605

  Kubori, Tomoko ^a,c   Matsushima, Yukiyasu ^a   Nakamura, Dai ^a   Uralil, Jaimol ^b   Lara Tejero, Maria ^b   Sukhan, Anand ^b   Galán, Jorge E ^b   Aizawa, Shin Ichi ^a  

^a TEIKYO UNIVERSITY   (Japan)

^b STONY BROOK UNIVERSITY   (United States)

^c NATIONAL INSTITUTE OF GENETICS   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIAL MEMBRANE; FLAGELLUM; GENE TRANSLOCATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN ISOLATION; PROTEIN PROCESSING; PROTEIN SECRETION; PROTEIN STRUCTURE; SALMONELLA TYPHIMURIUM;

BACTERIAL OUTER MEMBRANE PROTEINS; BACTERIAL PROTEINS; CELL MEMBRANE; CENTRIFUGATION, DENSITY GRADIENT; MACROMOLECULAR SUBSTANCES; MEMBRANE PROTEINS; MEMBRANE TRANSPORT PROTEINS; MICROSCOPY, ELECTRON; MICROSCOPY, IMMUNOELECTRON; PORINS; SALMONELLA TYPHIMURIUM;

EID: 0032562678     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.280.5363.602     Document Type: Article

References (29)

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12. Salmonella typhimurium strains were derivatives of SJW1103 [S. Yamaguchi, H. Fujita, A. Ishihara, S. Aizawa, R. M. Macnab, J. Bacteriol. 166,187 (1986)] or SL1344 [S. K. Hoiseth and B. A. Stocker, Nature 291, 238 (1981)]. The S. typhimurium flagellar mutants examined were ΔflhC, flgA, flgB, flgC, flgD, flgE, flgF, flgG, flhA, flhB, flhC, fliE, fliF, fliG, fliH, flil, fliJ, fliM, fliN, Δflio-fliR, fliP, ΔfliP-fliR, fliP, and fliQ.
13. T. Kubori et al., unpublished results.
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20. Polypeptides separated on standard SDS-polyacrytamide gels were electroblotted onto polyvinylidene difluoride membranes, and the bands of interest were applied to a Beckman LF3000 protein sequencer.
21. The amino acid sequences obtained from the different polypeptide species were as follows: 62 kD, SEKIPVTGSG; 52 kD, METSKEKTI; and 31 kD, CKDKD (19).
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25. The prgH and prgK genes were cloned from the wild-type strain of S. typhimurium SL1344 by polymerase change reaction, and derivative strains carrying nonpolar mutations in these genes were constructed by allele replacement (8). Salmonella typhimurium strains expressing a functional M45 epitope - tagged PrgH protein were constructed as described elsewhere [C. Collazo and J. E. Galán, Infect. Immun. 64, 3524 (1996)]. The M45 epitope tag consisted of 18 residues from the E4-6/7 protein of adenovirus (MDRSRDRLPPFETETRIL) (79) [S. Obert, R. J. O'Connor, S. Schmid, P. Hearing, Mol. Cell. Biol. 14, 1333 (1994)].
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27. Single-letter abbreviations for the amino acid residues are as follows: C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; I, IIe; K, Lys; L, Leu; M, Met; P, Pro; R, Arg; S, Ser; T, Thr; and V, Val.
28. T. Kubori, N. Shimamoto, S. Yamaguchi, K. Namba, S.-I. Aizawa, J. Mol. Biol. 226, 433 (1992).
29. We thank S. Yamaguchi for Salmonella flagellar mutant strains; M. Iwakura for amino acid sequence analysis; S. Makishima, T. Kubo, and N. Kobayashi for assistance in the needle preparation; and J. Bliska, R. Donis, and members of the Galán laboratory for critical reading of this manuscript. Supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture (S.-I.A.), the American Heart Association, and Public Health Service Grants from the NIH (J.E.G.).