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Results show that RNA tertiary interactions can aid in stabilizing binding of short oligonucleotides.
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Testa SM, Gryaznov SM, Turner DH: Antisense binding enhanced by tertiary interactions: binding of phosphorothioate and N3'-P5f phosphoramidate hexanucleotides to the catalytic core of a group I ribozyme from the mammalian pathogen Pneumocystis carinii. Biochemistry (1998) 37(26):9379-9385. Results show that RNA tertiary interactions can aid in stabilizing binding of short oligonucleotides.
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Introduces an in vitro method for identifying mRNA sites that are susceptible targets for oligonucleotide binding and subsequent RNase H cleavage.
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Ho SP, Bao Y, Lesher T. Malhotra R, Ma LY, Fluharty SJ, Sakai RR: Mapping of RNA accessible sites for antisense experiments with oligonucleotide libraries. Nat Biotechnol (1998) 16(1):59-63. Introduces an in vitro method for identifying mRNA sites that are susceptible targets for oligonucleotide binding and subsequent RNase H cleavage.
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Demonstrates the use of array technology to aid in the selection of antisense oligonucleotides.
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Müner N, Mir KU, Southern EM: Selecting effective antisense reagents on combinatorial oligonucleotide arrays. Nat Biotechnol (1997) 15(6):537-541. Demonstrates the use of array technology to aid in the selection of antisense oligonucleotides.
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Müner, N.1
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Reviews the difficulties and uncertainties faced in the antisense field.
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Branch AD: A good antisense molecule is hard to find. Trends Biochem Sc/(1998) 23(2):45-50. Reviews the difficulties and uncertainties faced in the antisense field.
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Describes recent progress in developing small molecular weight polyamides for sequence specific recognition of double stranded DNA.
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White S, Szewczyk JW, Turner JM. Baird EE, Dervan PB: Recognition of the four Watson-Crick base pairs in the DNA minor groove by synthetic ligands. Nature (1998) 391(6666):468-471. Describes recent progress in developing small molecular weight polyamides for sequence specific recognition of double stranded DNA.
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White, S.1
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11
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Sequenceselective recognition of DNA by strand displacement with a thymine-substituted polyamide
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Describes a full backbone replacement DNA mimic which has shown improved stability properties and potential as an antisense agent.
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Nielsen PE, Egholm M, Berg RH, Buchardt O: Sequenceselective recognition of DNA by strand displacement with a thymine-substituted polyamide. Science (1991) 254(5037):1497-1500. Describes a full backbone replacement DNA mimic which has shown improved stability properties and potential as an antisense agent.
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Nielsen, P.E.1
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Ho SP, Livanov V, Zhang W, U JH, Lesher T: Modification of pnosphorothioate oligonucleotides yields potent analogs with minimal toxicity for antisense experiments in the CNS. Molec Brain Res (1998) 62(1):1-11.
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Wagner RW, Matteucci MD. Grant D, Huang T, Froehler BC: Potent and selective inhibition of gene expression by an antisense heptanucleotide. Nat Biotechnol (1996) 14(7):840844. Results show that short oligonucleotides can provide useful selectivity.
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The first example that antisense oligonucleotides, even if unmodified, can be used to target mRNAs in the living mammalian brain. The approach was subsequently taken by a large number of investigators.
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Wahlestedt C, Pich EM, Koob GF, Yee F, Heilig M: Modulation of anxiety and neuropeptide Y-Y1 receptors by antisense oligodeoxynucleotides. Science (1993) 259(5094):528-531. The first example that antisense oligonucleotides, even if unmodified, can be used to target mRNAs in the living mammalian brain. The approach was subsequently taken by a large number of investigators.
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Wahlestedt, C.1
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19
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0029977448
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Antitumor activity of a phosphorothioate antisense oligodeoxynucleotide targeted against C-raf kinase
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Results show extensive mismatch evidence for sequence specific effects in vivo; in vitro results from an mRNA scan for susceptible target sites are provided.
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Monia BP, Johnston JF, Geiger T, Muller M, Fabbro D: Antitumor activity of a phosphorothioate antisense oligodeoxynucleotide targeted against C-raf kinase. Nat Med (1996) 2(6):668-675. Results show extensive mismatch evidence for sequence specific effects in vivo; in vitro results from an mRNA scan for susceptible target sites are provided.
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20
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0031907428
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Bcl-2 antisense therapy chemosensitises human melanoma in SCID mice
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Jansen B, Schlagbauer-Wadl H, Brown BD, Bryan RN, van Elsas A, Muller M, Wolff K, Eichler HG, Pehamberger H: bcl-2 antisense therapy chemosensitises human melanoma in SCID mice. War Med (1998) 4(2):232-234.
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21
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0031025249
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DNA antisense therapy for asthma in an animal model
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Effective antisense therapy for lung tissue using aerosol delivery. Demonstrates that lung tissue is an accessible target for antisense oligonucleotides.
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Nyce JW, Metzger WJ: DNA antisense therapy for asthma in an animal model. Nature (1997) 385(6618):721-725. Effective antisense therapy for lung tissue using aerosol delivery. Demonstrates that lung tissue is an accessible target for antisense oligonucleotides.
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Nature
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Metzger Wj, N.J.W.1
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22
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Prevention of graft coronary arteriosclerosis by arttisense cdk2 kinase oligonucleotide
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Suzuki J, Isobe M, Morishila R, Aoki M, Horie S, Okubo Y, Kaneda Y, Sawa Y, Matsuda H, Ogihara T, Sekiguchi M: Prevention of graft coronary arteriosclerosis by arttisense cdk2 kinase oligonucleotide. Nat Med(1997) 3(8):900-903.
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23
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Highly efficient cellular uptake of c-myb antisense oligonucleotides through specifically designed polymeric nanospheres
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Tondelli L, Ricca A, Laus M, Lelli M Citro G: Highly efficient cellular uptake of c-myb antisense oligonucleotides through specifically designed polymeric nanospheres. Nucl Acid Res (1998)26(23):5425-5431.
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24
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0031930787
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Integrin receptortargeted transfer peptides for efficient delivery of antisense oligodeoxynucleotides
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Results show efficient receptor-mediated uptake of antisense oligonucleotides.
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Bachmann AS, Surovoy A, Jung G, Moelling K: Integrin receptortargeted transfer peptides for efficient delivery of antisense oligodeoxynucleotides. J Mol Med (1998) 76(2): 126-132. Results show efficient receptor-mediated uptake of antisense oligonucleotides.
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J Mol Med
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Bachmann, A.S.1
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Derossi D, Chassaing G, Prochiantz A: Trojan peptides: the penetratin system for intracellular delivery. Trends Cell Biol (1998)8(2):84-87.
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27
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0031959657
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Genetic engineering of proteins with cell membrane permeability
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Results show the potential for oligonucleotide transport using carrier peptides.
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Rojas M, Donahue JP, Tan Z, Un YZ: Genetic engineering of proteins with cell membrane permeability. Nat Biotechnol (1998)16(4):370-375. Results show the potential for oligonucleotide transport using carrier peptides.
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Rojas, M.1
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DeMoor JM, Vincent MD, Collins OM, Koropatnick J: Antisense nucleic acids targeted to the thymidylate synthase (TS) mRNA translation start site stimulate TS gene transcription. Exp Cell Res (1998) 243(1 ):11 -21.
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Delihas N, Rokita SE, Zheng P: Natural antisense RNA/target RNA interactions: possible models for antisense oligo nucleotide drug design. Nat Biotechnol ( 1997) 15(8):751-753.
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31
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0031008403
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Antisense RNA control of plasmid R1 replication
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An example of the surprising secondary structures involved in natural ant'sense mechanisms.
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Malmgren C, Wagner EGH, Ehresmann C, Ehresmann B, Romby P: Antisense RNA control of plasmid R1 replication. The dominant product of the antisense RNA-mRNA binding is not a full RNA duplex. J Biol Chem (1997) 272(19):12508-12512. An example of the surprising secondary structures involved in natural ant'sense mechanisms.
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The Dominant Product of the Antisense RNA-mRNA Binding Is not a Full RNA Duplex. J Biol Chem
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32
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0029740475
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In vitro and in vivo reversal of multidrug resistance in a human leukemia-resistant cell line by mdrl antisense oligodeoxynucleotides
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An example of research showing that mdrl can be inhibited using antisense oligonucleotides.
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Cucco C, Calabretta B: In vitro and In vivo reversal of multidrug resistance in a human leukemia-resistant cell line by mdrl antisense oligodeoxynucleotides. Cancer Res (1996) 56(19):4332-4337. An example of research showing that mdrl can be inhibited using antisense oligonucleotides.
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Calabretta B, C.C.1
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Antisense inhibition of gene expression in bacteria by PNA targeted to mRNA
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Results demonstrate gene and sequence specific antisense in Escherichia coli using target site mutation/compensation experiments to prove mechanism.
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Good L, Nielsen PE: Antisense inhibition of gene expression in bacteria by PNA targeted to mRNA. Nat Biotechnol (1998) 16(4):355-358. Results demonstrate gene and sequence specific antisense in Escherichia coli using target site mutation/compensation experiments to prove mechanism.
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Nielsen Pe, G.L.1
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Outlines experimental guidelines for antisense studies.
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Fraser G, Wahlestedt C: Applications of antisense technology to both basic and clinical research. Exp Opin Invest Drugs (1995)4(7):637-646. Outlines experimental guidelines for antisense studies.
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Outlines guidelines for antisense studies and encourages publication of non-antisense effects.
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Crooke ST: Proof of mechanism of antisense drugs. Antisense Nucleic Acid Drug Dev(1996) 6(2):145-147. Outlines guidelines for antisense studies and encourages publication of non-antisense effects.
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Up-regulation of luciferase gene expression with antisense oligonucleotides: Implications and applications in functional assay development B/octeOTSfry(1998) 37(18):6235-6239. Results show increased gene expression associated with antisense inhibition of a regulatory gene
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Kang SH, Cho MJ, Kole R: Up-regulation of luciferase gene expression with antisense oligonucleotides: implications and applications in functional assay development B/octeOTSfry(1998) 37(18):6235-6239. Results show increased gene expression associated with antisense inhibition of a regulatory gene. Provides a functional expression system that may have advantages as an assay for comparative experiments in antisense design.
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Provides a Functional Expression System that May Have Advantages as an Assay for Comparative Experiments in Antisense Design.
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Kang, S.H.1
Cho, M.J.2
Kole, R.3
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