Adenovirus-mediated overexpression of uncoupling protein-2 in pancreatic islets of Zucker diabetic rats increases oxidative activity and improves β- cell function

Diabetes

Volumn 48, Issue 5, 1999, Pages 1020-1025

  Wang, May Yun ^a,b   Shimabukuro, Michio ^a,b   Lee, Young ^a,b   Trinh, Khiet Y ^c   Chen, Jin Long ^d   Newgard, Christopher B ^a   Unger, Roger H ^a,b,e  

^a UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

^b VA NORTH TEXAS HEALTH CARE SYSTEM   (United States)

^c UNIVERSITY OF TORONTO   (Canada)

^d AMGEN INC   (United States)

^e UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATE; GLUCOSE; LEPTIN; MESSENGER RNA; PALMITIC ACID; PREPROINSULIN; TRIACYLGLYCEROL; UNCOUPLING PROTEIN;

ADENOVIRUS; ADOLESCENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DIABETES MELLITUS; FATTY ACID OXIDATION; GENE OVEREXPRESSION; GENE TRANSFER; GLUCOSE OXIDATION; INSULIN RELEASE; LIPID COMPOSITION; MALE; NONHUMAN; OXIDATION; PANCREAS ISLET BETA CELL; PRIORITY JOURNAL; RAT;

ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATE; ADENOVIRIDAE; ANIMALS; DIABETES MELLITUS; GENE EXPRESSION; GENE TRANSFER TECHNIQUES; GLUCOSE; HUMANS; ION CHANNELS; ISLETS OF LANGERHANS; MALE; MEMBRANE TRANSPORT PROTEINS; MITOCHONDRIAL PROTEINS; OXIDATION-REDUCTION; PALMITIC ACID; PROINSULIN; PROTEIN PRECURSORS; PROTEINS; RATS; RATS, ZUCKER; TRIGLYCERIDES; UNCOUPLING AGENTS;

EID: 0032929816     PISSN: 00121797     EISSN: None     Source Type: Journal    
DOI: 10.2337/diabetes.48.5.1020     Document Type: Article

References (41)

1. Lee Y, Hirose H, Zhou YT, Esser V, McGany JD, Unger RH: Increased lipogenic of the islets of obese rats: a role in the pathogenesis of NIDDM. Diabetes 46:408-413, 1997
2. Stein DI, Esser V, Stevenson BE, Lane KK, Whiteside JH, Daniels MB, Chen S, McGarry JD: Essentiality of circulating fatty acids for glucose-stimulated insulin secretion in the fasted rat. J Clin Invest 97:2726-2735, 1996
3. Koyama K, Chen G, Lee Y, Unger RH: Tissue triglycerides, insulin resistance, and insulin production: implications for hyperinsulinemia of obesity. Am J Physiol 273 (Endocrinol Metab 36):E708-E713, 1997
4. Shimabukuro M, Koyama K, Chen G, Wang MY, Trieu F, Lee Y, Newgard CB, Unger RH: Direct antidiabetic effect of leptin through triglyceride depletion of tissues. Proc Natl Acad Sci U S A 94:4637-4464, 1997
5. Phillips MS, Liu QY, Hammond HA, Dugan V, Hey PJ, Caskey CT, Hess JF: Leptin receptor missense mutation in the fatty Zucker rat. Nat Genet 13:18-19, 1996
6. Iida M, Murakami T, Ishida K, Mizuno A, Kuwajima M, Shima K: Substitution at codon 269 (glutamine → proline) of the leptin receptor (OB-R) cDNA is the only mutation found in the Zucker fatty (fa/fa) rat. Biochem Biophys Res Commun 224:597-604, 1996
7. Unger RH: How obesity causes diabetes in Zucker diabetic fatty rats. Trends Endocrinol Metab 7:276-282, 1997
8. Unger RH: Lipotoxicity in the pathogenesis of obesity-dependent NIDDM: genetic and clinical implications. Diabetes 44:863-870, 1995
9. Zhou YT, Shimabukuro M, Koyama K, Lee Y, Wang MY, Trieu F, Newgard CB, Unger RH: Induction by leptin of uncoupling protein-2 and enzymes of fatty acid oxidation. Proc Natl Acad Sci U S A 94:6386-6390, 1997
10. Chen G, Koyama K, Yuan X, Lee Y, Zhou YT, O'Doherty R, Newgard CB, Unger RH: Disappearance of body fat in normal rats induced by adenovirus-mediated leptin gene therapy. Proc Natl Acad Sci U S A 93:14795-14799, 1996
11. Halaas JL, Gajiwala KS, Maffei M, Cohen SL, Chait BT, Rabinowitz D, Lallone RL, Burley SK, Friedman JM: Weight-reducing effects of the plasma protein encoded by the obese gene. Science 269:543-546, 1995
12. Campfield LA, Smith FJ, Guisez Y, Devos R, Burn P: Recombinant mouse OB protein: evidence for a peripheral signal linking adiposity and central neural networks. Science 269:546-549, 1995
13. Scarpace PJ, Matheny M, Pollock BH, Tumer N: Leptin increases uncoupling protein expression and energy expenditure. Am J Physiol 273 (Endocrinol Metab 36):E226-E230, 1997
14. Gomez-Foix AM, Coats WS, Baque S, Alan T, Gerard RD, Newgard CB: Adenovirus-mediated transfer of the muscle glycogen phosphorylase gene into hepatocytes confers altered regulation of glycogen metabolism. J Biol Chem 267:25129-251345, 1992
15. Becker TC, Noel RJ, Coats WS, Gomez-Foix AM, Alam T, Gerard RD, Newgard CB: Use of recombinant adenovirus for metabolic engineering of mammalian cells. Methods Cell Biol 43:161-189, 1994
16. Herz J, Gerard RD: Adenovirus-mediated transfer of low-density lipoprotein receptor gene acutely accelerates cholesterol clearance in normal mice. Proc Natl Acad Sci U S A 90:2812-2816, 1993
17. Hirose H, Lee Y, Inman LR, Nagasawa Y, Johnson JH, Unger RH: Defective fatty acid-mediated β-cell compensation in Zucker diabetic fatty rats. J Biol Chem 271:5633-5637, 1996
18. Koyama K, Chen G, Wang MY, Lee Y, Shimabukuro M, Newgard CB, Unger RH: β-cell function in normal rats made chronically hyperleptinemic by adenovirus-leptin gene therapy. Diabetes 46:1276-1280, 1997
19. Wang MY, Zhou YT, Newgard CB, Unger RH: A novel leptin receptor isoform in rats. FEBS Lett 392:87-90, 1996
20. Yalow RS, Berson SA: Immunoassay of endogenous plasma insulin in man. J Clin Invest 39:1157-1175, 1960
21. Goodwin GW, Ahmad F, Taegtmeyer H: Preferential oxidation of glycogen in isolated working rat heart. J Clin Invest 97:1409-1416, 1996
22. Newgard CB, Hirsch LJ, Foster DW, McGarry JD: Studies on the mechanism by which exogenous glucose is converted into liver glycogen in the rat: a direct or an indirect pathway? J Biol Chem 258:8046-8052, 1983
23. Bucher T: Uber ein phosphatubertragendes Garungsferment Biochem Biophys Acta 1:292-295, 1947
24. Adams H: Adenosine 5′-triphosphate determination with phosphoglycerate kinase. In Methods Enzymatic Analysis. HU Bergmeyer, Ed. New York, Academic, 1963, p. 539-543
25. Schultz V, Sussman I, Bokvist K, Tornheim K: Bioluminometric assay of ADP and ATP at high ATP/ADP ratios: assay of ADP after enzymatic removal of ATP. Anal Biochem 215:302-304, 1993
26. Zhou YT, Shimabukuro M, Lee Y, Koyama K, Trieu F, Unger RH: Leptin normalizes the impaired response of proinsulin mRNA to long-chain fatty acids in heterozygous Zucker diabetic fatty rats. J Biol Chem 272:25648-25651, 1997
27. m glucose transporters in non-insulin-dependent diabetes. Science 250:546-549, 1990
28. Fleury C, Neverova M, Collins S, Raimbault S, Champigny O, Levi-Meyrueis C, Bouillaud F, Seldin MF, Surwit RS, Ricquier D, Warden CH: Uncoupling protein-2: a novel gene linked to obesity and hyperinsulinemia. Nat Genet 15:269-272, 1997
29. Unger RH, Zhou Y-T, Orci L: Regulation of fat homeostasis in cells: novel role of leptin. Proc Natl Acad Sci U S A 96:2327-2732, 1999
30. Zhou YT, Shimabukuro M, Lee Y, Koyama K, Higa M, Ferguson T, Unger RH: Enhanced de novo lipogenesis in the leptin-unresponsive pancreatic islets of prediabetic Zucker diabetic fatty rats: role in the pathogenesis of lipotoxic diabetes. Diabetes 47:1904-1908, 1998
31. Wang MY, Koyama K, Shimabukuro M, Newgard CB, Unger RH: OB-Rb gene transfer to leptin-resistant islets reverses diabetogenic phenotype. Proc Natl Acad Sci U S A 95:714-718, 1998
32. Wang MY, Koyama K, Shimabukuro M, Mangelsdorf D, Newgard CB, Unger RH: Overexpression of leptin receptors in pancreatic islets of Zucker diabetic fatty rats restores GLUT-2, glucokinase, and glucose-stimulated insulin secretion. Proc Natl Acad Sci U S A 95:11921-11926, 1998
33. Malaisse WJ, Lebrun P, Sener A: Modulation of the insulinotropic action of glibenclamide and glimerpiride by nutrient secretagogues in pancreatic islets from normoglycemic and hyperglycemic rats. Biochem Pharmacol 45:1845-1849, 1993
34. Detimary P, Van den Berghe G, Henquin JC: Concentration dependence and time course of the effects of glucose on adenine and guanine nucleotides in mouse pancreatic islets. J Biol Chem 271:20559-20565, 1996
35. Civelek VN, Deeney JT, Kubik K, Schultz V, Tornheim K, Corkey BE: Temporal sequence of metabolic and ionic events in glucose-stimulated clonal pancreatic β-cells (HIT). Biochem J 315:1015-1019, 1996
36. + channels in mouse β-cells. J Clin Invest 91:871-880, 1993
37. + channels in rat pancreatic β-cells is modulated by ADP. FEBS 208:63-66, 1986
38. Newgard CB, McGarry JD: Metabolic coupling factors in pancreatic β-cell signal transduction. Annu Rev Biochem 64:689-719, 1983
39. Mertz RJ, Worley JF III, Spencer B, Johnson JH, Dukes ID: Activation of stimulus-secretion coupling in pancreatic β-cells by specific products of glucose metabolism. J Biol Chem 271:4838-1845, 1996
40. Noel RJ, Antinozzi PA, McGarry JD, Newgard CB: Engineering of glycerol-stimulated insulin secretion in islet β-cells. J Biol Chem 272:18621-18627, 1997
41. Megalasson MD, Matchinsky FM: Pancreatic islet glucose metabolism and regulation of insulin secretion. Diabetes Metab Rev 2:163-214, 1986