In vitro evolution of thermostable p53 variants

Protein Science

Volumn 8, Issue 4, 1999, Pages 731-740

  Matsumura, Ichiro ^a   Ellington, Andrew D ^a  

^a UNIVERSITY OF TEXAS AT AUSTIN   (United States)

Author keywords

Directed evolution; DNA shuffling; In vitro evolution; P53; Thermostability; Tumor suppressor

Indexed keywords

PROTEIN P53;

AMINO ACID SEQUENCE; ANTIGEN BINDING; ARTICLE; CARBOXY TERMINAL SEQUENCE; CRYSTALLOGRAPHY; ESCHERICHIA COLI; EVOLUTION; GENE EXPRESSION; GENE MUTATION; GENE THERAPY; PRIORITY JOURNAL; PROTEIN ANALYSIS; THERMOSTABILITY; TUMOR SUPPRESSOR GENE;

ESCHERICHIA COLI;

EID: 0032919288     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.8.4.731     Document Type: Article

References (36)

1. Alber T, Wozniak JA. 1985. A genetic screen for mutations that increase the thermal stability of phage T4 lysozyme. Proc Natl Acad Sci USA 82:741-750.
2. Anderson ME, Woelker B, Reed M, Wang P, Tegtmeyer P. 1997. Reciprocal interference between the sequence-specific core and nonspecific C-terminal DNA binding domains of p53: Implications for regulation. Mol Cell Biol 17:6255-6264.
3. Brachmann RK, Yu K, Eby Y, Pavletich NP, Boeke JD. 1998. Genetic selection of intragenic suppressor mutations that reverse the effect of common p53 cancer mutations. EMBO J 17:1847-1859
4. Cadwell RC, Joyce GF. 1992. Randomization of genes by PCR mutagenesis. PCR Methods Appl 2:28-33.
5. Cho Y, Gorina S, Jeffrey PD, Pavletich NP. 1994. Crystal structure of a p53 tumor suppressor-DNA complex: Understanding tumorigenic mutations. Science 265:346-355.
6. Donehower LA, Harvey M, Slagle BL, McArthur MJ, Montgomery CA Jr, Butel JS, Bradley A. 1992. Mice deficient for p53 are developmental normal but susceptible to spontaneous tumors. Nature 356:215-221.
7. El-Deiry WS, Kern SE, Pietenpol JA, Kinzler KW, Vogelstein B. 1992. Definition of a consensus binding site for p53. Nat Genet 1:45-49.
8. Funk WD, Pak DT, Karas RH, Wright WE, Shay JW. 1992. A transcriptionally active DNA binding site for human p53 protein complexes. Mol Cell Biol 12:2866-2871.
9. Grimm E, Arbuthnot P. 1995. Rapid purification of recombinant tag DNA polymerase by freezing and high temperature thawing of bacterial expression cultures. Nucleic Acids Res 23:4518-4519.
10. Hansen S, Hupp TR, Lane DP. 1996. Allosteric regulation of the thermostability and DNA binding activity of human p53 by specific interacting proteins. J Biol Chem 271:3917-3924.
11. Hollstein M, Sidransky D, Vogelstein B, Harris CC. 1991. p53 mutations in human cancers. Science 253:49-53.
12. Hupp TR, Lane DP. 1994. Allosteric activation of latent p53 tetramers. Curr Biol 4:865-875.
13. Hupp TR, Meek DW, Midgley CA, Lane DP. 1992. Regulation of the specific DNA binding function of p53. Cell 71:875-886.
14. Inoue H, Nojima H, Okayama H. 1990. High efficiency transformation of Escherichia coli with plasmids. Gene 96:23-28.
15. Jeffrey PD, Gorina S, Pavletich NP. 1995. Crystal structure of the tetramerization domain of the p53 tumor suppressor at 1.7 angstroms. Science 267:1498-1502.
16. Kern SE, Pietenpol JA, Thiagalingam S, Seymour A, Kinzler KW, Vogelstein B. 1992. Oncogenic forms of p53 inhibit p53-regulated gene expression. Science 256:827-830.
17. King PV, Blakesley RW. 1986. Optimizing DNA ligations for transformations. Focus 8:30-32.
18. Ko LJ, Prives C. 1996. p53: Puzzle and paradigm. Genes Dev 10:1054-1072.
19. Lee W, Harvey TS, Yin Y, Yau P, Litchfield D, Arrowsmith CH. 1994. Solution structure of the tetrameric minimum transforming domain of p53. Nat Struct Biol 1:877-890.
20. Leung DW, Chen E, Goeddel DV. 1989. A method for random mutagenesis of a defined DNA segment using a modified polymerase chain reaction. Technique 1:11-15.
21. Levine AJ. 1997. p53, The cellular gatekeeper for growth and division. Cell 88:323-331.
22. Milner J. 1995. Flexibility: The key to p53 function? Trends Biochem Sci 20: 49-51.
23. Nikolova PV, Henckel J, Lane DP, Fersht AR. 1998. Semirational design of active tumor suppressor p53 DNA binding domain with enhanced stability. Proc Natl Acad Sci USA 95(25):14675-14680.
24. Okorokov AL, Ponchel F, Milner J. 1997. Induced N- and C-terminal cleavage of p53: A core fragment of p53, generated by interaction with damaged DNA, promotes cleavage of the N-terminus of full-length p53, whereas ssDNA induces C-terminal cleavage of p53. EMBO J 16:6008-6017.
25. Reed M, Woelker B, Wang P, Wang Y, Anderson ME, Tegtmeyer P. 1995. The C-terminal domain of p53 recognizes DNA damaged by ionizing radiation. Proc Natl Acad Sci USA 92:9455-9459.
26. Roth JA, Nguyen D, Lawrence DD, Kemp BL, Carrasco CH, Ferson DZ, Hong WK, Komaki R, Lee JJ, Nesbitt JC. 1996. Retrovirus-mediated wild-type p53 gene transfer to tumors of patients with lung cancer. Nat Med 2:985-991.
27. Sakaguchi K, Herrera JE, Saito S, Miki T, Bustin M, Vassilev A, Anderson CW, Appella E. 1998. DNA damage activates p53 through a phosphorylation-acetylation cascade. Genes Dev 12:2831-2841.
28. Scharer E, Iggo R. 1992. Mammalian p53 can function as a transcription factor in yeast. Nucleic Acids Res 20:1539-1545.
29. Shaw P, Freeman J, Bovey R, Iggo R. 1996. Regulation of specific DNA binding by p53: Evidence for a role for O-glycosylation and charged residues at the carboxy-terminus. Oncogene 12:921-930.
30. Singh H, Clerc RG, LeBowitz JH. 1989. Molecular cloning of sequence-specific DNA binding proteins using recognition site probes. Biotechniques 7:252-261.
31. Soussi T, May P. 1996. Structural aspects of the p53 protein in relation to gene evolution: A second look. J Mol Biol 260:623-637.
32. Stemmer WPC. 1994. DNA shuffling by random fragmentation and reassembly: In vitro recombination for molecular evolution. Proc Natl Acad Sci USA 91:10747-10751.
33. Varley JM, McGown G, Thorncroft M, Cochrane S, Morrison P, Woll P, Kelsey AM, Mitchell EL, Boyle J, Birch JM. 1996. A previously undescribed mutation within the tetramerization domain of TP53 in a family with Li-Fraumeni syndrome. Oncogene 12:2437-2442.
34. Wieczorek AM, Waterman JL, Waterman MJ, Halazonetis TD. 1996. Structure-based rescue of common tumor-derived p53 mutants. Nat Med 2:1143-1146.
35. Zhang XJ, Baase WA, Shoichet BK, Wilson KP, Matthews BW. 1995. Enhancement of protein stability by the combination of point mutations in T4 lysozyme is additive. Protein Eng 8:1017-1022.
36. Zhao H, Arnold FH. 1997. Functional and nonfunctional mutations distinguished by random recombination of homologous genes. Proc Natl Acad Sci USA. 94:7997-8000.