Flow cytometric detection of antigen-specific cytokine responses in lung T cells in a murine model of pulmonary inflammation

Journal of Immunological Methods

Volumn 226, Issue 1-2, 1999, Pages 105-118

  Winterrowd, Greg E ^a   Chin, Jia En ^a  

^a Pharmacia Upjohn 301 Henrietta St   (United States)

Author keywords

Allergic lung inflammation; Antigen specific T cells; Cytokine; Flow cytometry; Intracellular immunofluorescence

Indexed keywords

BREFELDIN A; GAMMA INTERFERON; INTERLEUKIN 2; INTERLEUKIN 4; INTERLEUKIN 5; IONOMYCIN; MONOCLONAL ANTIBODY CD3; PHORBOL 13 ACETATE 12 MYRISTATE; TUMOR NECROSIS FACTOR ALPHA;

ALLERGY; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ANTIGEN ANTIBODY REACTION; ANTIGEN SPECIFICITY; ARTICLE; CELL INFILTRATION; CELL STIMULATION; FLOW CYTOMETRY; IMMUNE RESPONSE; IMMUNOFLUORESCENCE TEST; NONHUMAN; PNEUMONIA; PRIORITY JOURNAL; T LYMPHOCYTE;

ANIMALS; ANTIGENS; ASTHMA; CD4-POSITIVE T-LYMPHOCYTES; CD8-POSITIVE T-LYMPHOCYTES; CULTURE MEDIA; CYTOKINES; FEMALE; FLOW CYTOMETRY; LUNG; LYMPHOCYTE ACTIVATION; MICE; MICE, INBRED C57BL; PNEUMONIA;

ANIMALIA; MURINAE;

EID: 0032811720     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(99)00049-6     Document Type: Article

References (40)

1. Ackerman V., Marini M., Vittori E., Bellini A., Vassali G., Mattoli S. Detection of cytokines and their cell sources in bronchial biopsy specimens from asthmatic patients. Chest. 105:1994;687.
2. Assenmacher M., Schmitz J., Radbruch A. Flow cytometric determination of cytokines in activated murine T helper lymphocytes: expression of interleukin-10 in interferon-γ and in interleukin-4-expressing cells. Eur. J. Immunol. 24:1994;1097.
3. Barnes P.J. Cytokines as mediators of chronic asthma. Am. J. Respir. Crit. Care Med. 150:1994;S42.
4. Coyle A.J., Le Gros G., Bertrand C., Tsuyuki S., Heusser C., Kopf M., Anderson G.P. Interleukin-4 is required for the induction of lung Th2 mucosal immunity. Am. J. Respir. Cell Mol. Biol. 13:1995;54.
5. + cells can switch to interleukin-5 production and induce airway eosinophilia. J. Exp. Med. 181:1995;1229.
6. Coyle A.J., Wagner K., Bertrand C., Tsuyuki S., Bews J., Heusser C. Central role of immunoglobulin (Ig) E in the induction of lung eosinophil infiltration and T helper 2 cell cytokine production: inhibition by a non-anaphylactogenic anti-IgE antibody. J. Exp. Med. 183:1996;1303.
7. Doucet C., Brouty-Boye D., Pottin-Clemenceau C., Canonica G.W., Jasmin C., Azzarone B. Interleukin (IL) 4 and IL-13 act on human lung fibroblasts. Implication in asthma. J. Clin. Invest. 101:1998;2129.
8. Galli S.J. Complexity and redundancy in the pathogenesis of asthma: reassessing the roles of mast cells and T cells. J. Exp. Med. 186:1997;343.
9. 2 cytokine production and eosinophil accumulation in airways of antigen-challenged allergic mice. Clin. Immunol. Immunopathol. 75:1997;75.
10. Gonzalo J.-A., Lloyd C.M., Kremer L., Finger E., Martinez-A C., Siegelman M.H., Cybulsky M., Gutierrez-Ramos J.C. Eosinophil recruitment to the lung in a murine model of allergic inflammation: the role of T cells, chemokines, and adhesion molecules. J. Clin. Invest. 98:1996;2332.
11. + T cells in the development of airway hyperresponsiveness in a murine model of airway sensitization. J. Exp. Med. 183:1996;1719.
12. Hatfield C.A., Brashler J.R., Winterrowd G.E., Bell F.P., Griffin R.L., Fidler S.F., Kolbasa K.P., Shull K.L., Mobley J.L., Richards I.M., Chin J.E. Intercellular adhesion molecule-1-deficient mice have normal antibody responses, but impaired leukocyte recruitment. Am. J. Physiol. 273:1997;L513.
13. Hogan S.P., Foster P.S. Cytokines as targets for the inhibition of eosinophilic inflammation. Pharmacol. Ther. 74:1997;259.
14. + T cells play a pivotal role in aeroallergen-induced eosinophilia, bronchial hyperreactivity, and lung damage in mice. Am. J. Respir. Crit. Care Med. 157:1998;210.
15. Hussell T., Spender L.C., Georgiou A., O'Garra A., Openshaw P.J.M. Th1 and Th2 cytokine induction in pulmonary T cells during infection with respiratory syncytial virus. J. Gen. Virol. 77:1996;2447.
16. Hussell T., Khan U., Openshaw P. IL-12 treatment attenuates T helper cell type 2 and B cell responses but not improve vaccine-enhanced lung illness. J. Immunol. 159:1997;328.
17. Jason J., Larned J. Single-cell cytokine profiles in normal humans: comparision of flow cytometric reagents and stimulation protocols. J. Immunol. Meth. 207:1997;13.
18. Kay A.B. T cells as orchestrators of asthmatic response. Ciba Found. Symp. 206:1997;57.
19. Kennedy J.D., Hatfield C.A., Fidler S.F., Winterrowd G.E., Chin J.E., Richards I.M. Phenotypic characteristics of T lymphocytes emigrating into lung tissue and the airway lumen following antigen inhalation in sensitized mice. Am. J. Respir. Cell Mol. Biol. 12:1995;613.
20. Klemm A., Tschernig T., Krug N., Pabst R. Lymphocyte subsets in distinct lung compartments show a different ability to produce interferon-gamma (IFN-γ) during a pulmonary immune response. Clin. Exp. Immunol. 113:1998;252.
21. Krinzman S.J., De Sanctis G.T., Cernadas M., Kobzik L., Listman J.A., Christiani D.C., Perkins D.L., Finn P.W. T cell activation in a murine model of asthma. Am. J. Physiol. 271:1996;L476.
22. Krouwels F.H., Nocker R.E.T., Snoek M., Lutter R., van der Zee J.S., Weller F.R., Hansen H.M., Out T.A. Immunocytochemical and flow cytofluorimetric detection of intracellular IL-4, IL-5 and IFN-γ: applications using blood- and airway-derived cells. J. Immunol. Meth. 203:1997;89.
23. Krug N., Madden J., Redington A.E., Lackie P., Djukanovic R., Schauer U., Holgate S.T., Frew A.J., Howarth P.H. T-cell cytokine profile evaluated at the single cell level in BAL and blood in allergic asthma. Am. J. Respir. Cell Mol. Biol. 14:1996;319.
24. Krug N., Jung T., Napp U., Wagner K., Schultze-Werninghaus G., Heusser C., Rieger C.H.L., Schauer U., Fabel H. Frequencies of T cells expressing interleukin-4 and interleukin-5 in atopic asthmatic children. Am. J. Respir. Crit. Care Med. 158:1998;754.
25. Krzesicki R.F., Winterrowd G.E., Brashler J.R., Hatfield C.A., Griffin R.L., Fidler S.F., Kolbasa K.P., Shull K.P., Richards I.M., Chin J.E. Identification of cytokine and adhesion molecule mRNA in murine lung tissue and isolated T cells and eosinophils by semi-quantitative reverse transcriptase-polymerase chain reaction. Am. J. Respir. Cell Mol. Biol. 16:1997;693.
26. Kuperman D., Schofield B., Wills-Karp M., Grusby M.J. Signal transducer and activator of transcription factor 6 (Stat6)-deficient mice are protected from antigen-induced airway hyperresponsiveness and mucus production. J. Exp. Med. 187:1998;939.
27. Marone G. Asthma: recent advances. Immunol. Today. 19:1998;5.
28. Murphy E., Shibuya K., Hosken N.A., Openshaw P.J.M., Manio V., Davis K., Murphy K., O'Garra A. Reversibility of T helper 1 and T helper 2 populations is lost after long term stimulation. J. Exp. Med. 183:1996;901.
29. Nadel J.A., Busse W.W. Asthma. Am. J. Respir. Crit. Care Med. 157:1998;S130.
30. Nourshargh S. Adhesion molecules and asthma. J. Pharm. Pharmacol. 49:1997;33. Suppl. 3.
31. Olivenstein R., Renzi P.M., Yang J.P., Rossi P., Laberge S., Waserman S., Martin J.G. Depletion of OX-8 lymphocytes from the blood and airways using monoclonal antibodies enhances the late airway responses in rats. J. Clin. Invest. 92:1993;1477.
32. Openshaw P., Murphy E.E., Hosken N.A., Maino V., Davis K., Murphy K., O'Garra A. Heterogeneity of intracellular cytokine synthesis at the single-cell level in polarized T helper 1 and T helper 2 population. J. Exp. Med. 182:1995;1357.
33. Picker L.J., Singh M.K., Zdraveski Z., Treer J.R., Waldrop S.L., Bergstresser P.R., Maino V.C. Direct demonstration of cytokine synthesis heterogeneity among human memory/effector T cells by flow cytometry. Blood. 86:1995;1408.
34. Prussin C., Metcalfe D.D. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J. Immunol. Meth. 188:1995;117.
35. H2 type cytokine mRNA expression, and eosinophil recruitment in bronchoalveolar lavage after allergen inhalation challenge in patients with atopic asthma. J. Allergy Clin. Immunol. 92:1993;313.
36. Suni M., Picker L.J., Maino V.C. Detection of antigen-specific T cell cytokine expression in whole blood by flow cytometry. J. Immunol. Meth. 212:1998;89.
37. Tang C., Rolland J.M., Ward C., Quan B., Walters E.H. IL-5 production by bronchoalveolar lavage and peripheral blood mononuclear cells in asthma and atopy. Allergy. 10:1997;624.
38. Waldrop S.L., Pitcher C.J., Peterson D.M., Maino V.C., Picker L.J. Determination of antigen-specific memory/effector CD4+ T cell frequencies by flow cytometry. Evidence for a novel, antigen-specific homeostatic mechanism in HIV-associated immunodeficiency. J. Clin. Invest. 99:1997;1739.
39. Watanabe A., Mishima H., Renzi P.M., Xu L.-J., Hamid Q., Martin J.G. Transfer of allergic airway responses with antigen-primed CD4+ but not CD8+ T cells in Brown Norway rats. J. Clin. Invest. 96:1995;1303.
40. + T cells, eosinophils, and mast cells in bronchial biopsies obtained from atopic and nonatopic (intrinsic) asthmatics. J. Immunol. 158:1997;3539.