Immunocytochemical and flow cytofluorimetric detection of intracellular IL-4, IL-5 and IFN-γ: Applications using blood- and airway-derived cells

Journal of Immunological Methods

Volumn 203, Issue 1, 1997, Pages 89-101

  Krouwels, F H ^a   Nocker, R E T ^a   Snoek, M ^a   Lutter, R ^a   Van Der Zee, J S ^a   Weller, F R ^a   Jansen, H M ^a   Out, T A ^a,b,c  

^a UNIVERSITY OF AMSTERDAM   (Netherlands)

^b Clin and Laboratory Immunology Unit   (Netherlands)

^c ACADEMIC MEDICAL CENTER   (Netherlands)

Author keywords

Asthma; Bronchoalveolar lavage; Flow cytofluorimetry; Immunocytochemistry; Intracellular cytokine; Sarcoidosis

Indexed keywords

GAMMA INTERFERON; INTERLEUKIN 4; INTERLEUKIN 5;

ADULT; ARTICLE; ASTHMA; CLINICAL ARTICLE; CONTROLLED STUDY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; HUMAN EXPERIMENT; IMMUNOCYTOCHEMISTRY; LUNG LAVAGE; PRIORITY JOURNAL; SARCOIDOSIS;

BRONCHOALVEOLAR LAVAGE FLUID; CLONE CELLS; FLOW CYTOMETRY; HUMANS; HYPERSENSITIVITY, IMMEDIATE; IMMUNOHISTOCHEMISTRY; INTERFERON TYPE II; INTERLEUKIN-4; INTERLEUKIN-5; INTRACELLULAR FLUID; LEUKOCYTES, MONONUCLEAR; SARCOIDOSIS; STAINING AND LABELING; T-LYMPHOCYTES; TH1 CELLS; TH2 CELLS; TIME FACTORS;

EID: 0030975273     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(97)00016-1     Document Type: Article

References (35)

1. Altman, D.G., 1995. Some common problems in medical research. In: Altman, D.G. (Ed.), Practical statistics for medical research. Chapman and Hall, London, UK, pp. 403.
2. Andersson, U., Hallden, G., Persson, U., Hed, J., Möller, G., DeLey, M., 1988. Enumeration of IFN-gamma-producing cells by flow cytometry. J. Immunol. Methods 112, 139.
3. Andersson, U., Andersson, J., Lindfors, A., Wagner, K., Möller, G., Heusser, C.H., 1990. Simultaneous production of interleukin 2, interleukin 4 and interferon-gamma by activated human blood lymphocytes. Eur. J. Immunol. 20, 1591.
4. Broide, D.H., Lotz, M., Cuomo, A.J., Coburn, D.A., Federman, E.C., Wasserman, S.I., 1992. Cytokines in symptomatic asthma airways. J. Allergy Clin. Immunol. 89, 958.
5. Costabel, U., Bross, K.J., Ruhle, K.H., Löhr, G.W., Matthys, H., 1985. Ia-like antigens on T-cells and their subpopulations in pulmonary sarcoidosis and in hypersensitivity pneumonitis. Am. Rev. Respir. Dis. 131, 337.
6. Djukanovic, R., Lai, C.K.W., Wilson, J.W., Britten, K.M., Wilson, S.J., Roche, W.R., Howarth, P.H., Holgate, S.T., 1992. Bronchial mucosal manifestations of atopy: a comparison of markers of inflammation between atopic asthmatics, atopic nonasthmatics and healthy controls. Eur. Respir. J. 5, 538.
7. Dolhain, R.J.E.M., Andersson, U., ter Haar, N.T., Brinkman, B.M.N., Verweij, C.L., Daha, M.R., Breedveld, F.C., Miltenburg, A.M.M., 1993. Detection of intracellular interferon-γ by light microscopy using an immunoperoxidase technique: correlation with the corresponding mRNA and protein product. J. Leukocyte Biol. 54, 545.
8. Du Bois, R.M., Kirby, M., Balbi, B., Saltini, C., Crystal, R.G., 1992. T-lymphocytes that accumulate in the lung in sarcoidosis have evidence of recent stimulation of the T-cell antigen receptor. Am. Rev. Respir. Dis. 145, 1205.
9. Elson, L.H., Nutman, T.B., Metcalfe, D.D., Prussin, C., 1995. Flow cytometric analysis for cytokine production identifies Thelper1, Thelper2, and Thelper0 cells within the human CD4+CD27- lymphocyte subpopulation. J. Immunol. 154, 4294.
10. Garlepp, M.J., Rose, A.H., Bowman, R.V., Mavaddat, N., Dench, J., Holt, B.J., Baron-Hay, M., Holt, P.G., Robinson, B.W.S., 1992. A clonal analysis of lung T cells derived by bronchoalveolar lavage of healthy individuals. Immunology 77, 31.
11. Hol, B.E.A., Hintzen, R.Q., Van Lier, R.A.W., Alberts, C., Out, T.A., Jansen, H.M., 1993. Soluble and cellular markers of T-cell activation in patients with pulmonary sarcoidosis. Am. Rev. Respir. Dis. 148, 643.
12. Hol, B.E.A., Krouwels, F.H., Bruinier, B., Reijneke, R.M.R., Mengelers, H.J.J., Koederman, L., Jansen, H.M., Out, T.A., 1992. Cloning of T-lymphocytes from broncho-alveolar lavage fluid. Am. J. Respir. Cell Mol. Biol. 7, 523.
13. Huang, S.-K., Krishnaswamy, G., Su, S.-N., Xiao, H.-Q., Liu, M.C., 1994. Qualitative and quantitative analysis of cytokine transcripts in the bronchoalveolar lavage cells of patients with asthma. Ann. New York Acad. Sci. 725, 110.
14. Jansen, H.M., Schutte, A.J.H., Elema, J.D., Van der Giessen, M., Peset Reig, R., Leeuwen, M.A.V., Sluiter, H.J., The, T.H., 1984. Local immune complexes and inflammatory response in patients with chronic interstitial pulmonary disorders associated with collagen vascular diseases. Clin. Exp. Immunol. 56, 311.
15. Jung, T., Schauer, U., Heusser, C., Neumann, C., Rieger, C., 1993. Detection of intracellular cytokines by flow cytometry. J. Immunol. Methods 159, 197.
16. 2+ for production of IFN-gamma by a cytolytic T cell clone is post-transcriptional. J. Immunol. 146, 2382.
17. Krouwels, F.H., Hol, B.E.A., Bruinier, B., Lutter, R., Jansen, H.M., Out, T.A., 1996. Cytokine production by T-cell clones from bronchoalveolar lavage fluid of patients with asthma and healthy controls. Eur. Respir. J., in press.
18. Labalette-Houache, M., Torpier, G., Capron, A., Dessaint, J.P., 1991. Improved permeabilization procedure for flow cytometric detection of internal antigens; analysis of IL-2 production. J. Immunol. Methods 138, 143.
19. Lederer, J.A., Liou, J.S., Todd, M.D., Glimcher, L.H., Lichtman, A.H., 1994. Regulation of cytokine gene expression in T helper subsets. J. Immunol. 152, 77.
20. Openshaw, P., Murphy, E.E., Hosken, N.A., Maino, V., Davis, D., Murphy, K., O'Gara, A., 1995. Heterogeneity of intracellular cytokine synthesis at the single-cell level in polarized T helper 1 and T helper 2 populations. J. Exp. Med. 182, 1357.
21. Pinkston, P., Bitterman, P.B., Crystal, R.G., 1983. Spontaneous release of IL-2 by lung T-lymphocytes in active pulmonary sarcoidosis. New Engl. J. Med. 308, 793.
22. Prior, C., Knight, R.A., Herold, M., Ott, G., Spiteri, M.A., 1996. Pulmonary sarcoidosis: patterns of cytokine release in vitro. Eur. Respir. J. 9, 47.
23. Robinson, B.W.S., McLemore, T.L., Crystal, R.G., 1985. Gamma interferon is spontaneous released by alveolar macrophages and lung T lymphocytes in patients with pulmonary sarcoidosis. J. Clin. Invest. 75, 1488.
24. H2-like bronchoalveolar T-lymphocyte population in atopic asthma. New Engl. J. Med. 326, 298.
25. Romagnani, S., 1994. Lymphokine production by human T cells in disease states. Ann. Rev. Immunol. 12, 227.
26. Sander, B., Andersson, J., Andersson, U., 1991. Assessment of cytokines by immunofluorescence and the paraformaldehydesaponin procedure. Immunol. Rev. 119, 65.
27. Schmitz, J., Assenmacher, M., Radbruch, A., 1993. Regulation of T helper cell cytokine expression: Functional dichotomy of antigen-presenting cells. Eur. J. Immunol. 23, 191.
28. Tang, M.L.K., Varigos, G., Kemp, A.S., 1994. Reduced interferon-gamma (IFN-gamma) secretion with increased IFN-gamma mRNA expression in atopic dermatitis: evidence for a post-transcriptional defect. Clin. Exp. Immunol. 97, 483.
29. Van der Meide, P.H., Dubbeld, M., Schellekens, H., 1985. Monoclonal antibodies to human immune interferon and their use in a sensitive solid-phase ELISA. J. Immunol. Methods 79, 293.
30. Van Neerven, R.J.J., Van 't Hof, W., Ringrose, J.H., Jansen, H.M., Aalberse, R.C., Wierenga, E.A., Kapsenberg, M.L., 1993. T cell epitopes of house dust mite major allergen Der p II. J. Immunol. 151, 2326.
31. Walker, C., Bode, E., Boer, L., Hansel, T.T., Blaser, K., Virchow, J.-C. Jr., 1992. Allergic and nonallergic asthmatics have distinct patterns of T-cell activation and cytokine production in peripheral blood and bronchoalveolar lavage. Am. Rev. Respir. Dis. 146, 109.
32. Walker, C., Bauer, W., Braun, R.K., Menz, G., Braun, P., Schwarz, F., Hansel, T.T., Villiger, B., 1994. Activated T cells and cytokines in bronchoalveolar lavages from patients with various lung diseases associated with eosinophilia. Am. J. Respir. Crit. Care Med. 150, 1038.
33. Warren, C.J., Howell, W.M., Bhambhani, M., Cawley, M.I.D., Smith, J.L., 1991. An investigation of T-cell subset phenotype and function in the rheumatoid synovium using in situ hybridization for IL-2 mRNA. Immunology 72, 250.
34. Ying, S., Durham, S.R., Corrigan, C.J., Hamid, Q., Kay, A.B., 1995. Phenotype of cells expressing mRNA for TH2-type (interleukin-4 and interleukin-5) and TH1-type (interleukin-2 and interferon gamma) cytokines in bronchoalveolar lavage and bronchial biopsies from atopic asthmatic and normal control subjects. Am. J. Respir. Cell Mol. Biol. 12, 477.
35. Zangrilli, J.G., Shaver, J.R., Cirelli, R.A., Cho, S.K., Garlisi, C.G., Falcone, A., Cuss, F.M., Fish, J.E., Peters, S.P., 1995. sVCAM-1 levels after segmental antigen challenge correlate with eosinophil influx, IL-4 and IL-5 production, and the late phase response. Am. J. Respir. Crit. Care Med. 151, 1346.