A novel allele of RAD52 that causes severe DNA repair and recombination deficiencies only in the absence of RAD51 or RAD59

Genetics

Volumn 153, Issue 3, 1999, Pages 1117-1130

  Bai, Yun ^a   Davis, Allison P ^a   Symington, Lorraine S ^a  

^a Columbia University ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FUNGAL DNA;

ALLELE; ARTICLE; DNA REPAIR; FUNGUS MUTANT; GENE MUTATION; GENE OVEREXPRESSION; GENETIC RECOMBINATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RADIOSENSITIVITY; REPORTER GENE; SACCHAROMYCES CEREVISIAE; SPOROGENESIS; YEAST;

ALLELES; AMINO ACID SUBSTITUTION; DNA REPAIR; DNA-BINDING PROTEINS; FUNGAL PROTEINS; GAMMA RAYS; GENOTYPE; MUTAGENESIS, SITE-DIRECTED; POINT MUTATION; POLYMERASE CHAIN REACTION; RAD51 RECOMBINASE; RAD52 DNA REPAIR AND RECOMBINATION PROTEIN; RECOMBINATION, GENETIC; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SUPPRESSION, GENETIC;

FUNGI; SACCHAROMYCES CEREVISIAE;

EID: 0032712707     PISSN: 00166731     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (68)

1. ADZUMA, K., T. OGAWA and H. OGAWA, 1984 Primary structure of the RAD52 gene in Saccharomyces cerevisiae. Mol. Cell. Biol. 4: 2735-2744.
2. AJIMURA, M., S. H. LEEM and H. OGAWA, 1993 Identification of new genes required for meiotic recombination in Saccharomyces cerevisiae. Genetics 133: 51-66.
3. BAI, Y., and L. S. SYMINGTON, 1996 A Rad52 homolog is required for RAD51-independent mitotic recombination in Saccharomyces cerevisiae. Genes Dev. 10: 2025-2037.
4. BENDIXEN, C., I. SUNJEVARIC, R. BAUCHWITZ and R. ROTHSTEIN, 1994 Identification of a mouse homologue of the Saccharomyces cerevisiae recombination and repair gene, RAD52. Genomics 23: 300-303.
5. BENSON, F. E., P. BAUMANN and S. C. WEST, 1998 Synergistic actions of Rad51 and Rad52 in recombination and DNA repair. Nature 391: 401-404.
6. BEZZUBOVA, O. Y., H. SCHMIDT, K. OSTERMANN, W. D. HEYER and J. M. BUERSTEDDE, 1993 Identification of a chicken RAD52 homologue suggests conservation of the RAD52 recombination pathway throughout the evolution of higher eukaryotes. Nucleic Acids Res. 21: 5945-5949.
7. BOUNDY-MILLS, K. L., and D. M. LIVINGSTON, 1993 A Saccharomyces cerevisiae RAD52allele expressing a C-terminal truncation protein: activities and intragenic complementation of missense mutations. Genetics 133: 39-49.
8. DONOVAN, J. W., G. T. MILNE and D. T. WEAVER, 1994 Homotypic and heterotypic protein associations control Rad51 function in double-strand break repair. Genes Dev. 8: 2552-2562.
9. FIRMENICH, A. A., M. ELIAS-ARNAZ and P. BERG, 1995 A novel allele of Saccharomyces cerevisiae RFA1 that is deficient in recombination and repair and suppressible by RAD52. Mol. Cell. Biol. 15: 1620-1631.
10. GAME, J. C., and R. K. MORTIMER, 1974 A genetic study of x-ray sensitive mutants in yeast. Mutat. Res. 24: 281-292.
11. GAME, J. C., T. J. ZAMB, R. J. BRAUN, M. RESNICK and R. M. ROTH, 1980 The role of the radiation (rad) genes in meiotic recombination in yeast. Genetics 94: 51-68.
12. HABER, J. E., 1995 In vivo biochemistry: physical monitoring of recombination induced by site-specific endonucleases. Bioessays 17: 609-620.
13. HAYS, S. L., A. A. FIRMENICH and P. BERG, 1995 Complex formation in yeast double-strand break repair: participation of Rad51, Rad52, Rad55, and Rad57 proteins. Proc. Natl. Acad. Sci. USA 92: 6925-6929.
14. HAYS, S. L., A. A. FIRMENICH, P. MASSEY, R. BANERJEE and P. BERG, 1998 Studies of the interaction between Rad52 protein and the yeast single-stranded DNA binding protein RPA. Mol. Cell. Biol. 18: 4400-4406.
15. HO, K. S., 1975 The gene dosage effect of the rad52 mutation on X-ray survival curves of tetraploid yeast strains. Mutat. Res. 33: 165-172.
16. HOEKSTRA, M. F., T. NAUGHTON and R. E. MALONE, 1986 Properties of spontaneous mitotic recombination occurring in the presence of the rad52-1 mutation of Saccharomyces cerevisiae. Genet. Res. 48: 9-17.
17. HUANG, K. N., and L. S. SYMINGTON, 1994 Mutation of the gene encoding protein kinase C 1 stimulates mitotic recombination in Saccharomyces cerevisiae. Mol. Cell. Biol. 14: 6039-6045.
18. IVANOV, E. L., V. G. KOROLEV and F. FABRE, 1992 XRS2, a DNA repair gene of Saccharomyces cerevisiae, is needed for meiotic recombination. Genetics 132: 651-664.
19. IVANOV, E. L., N. SUGAWARA, J. FISHMAN-LOBELL and J. E. HABER, 1996 Genetic requirements for the single-strand annealing pathway of double-strand break repair in Saccharomyces cerevisiae. Genetics 142: 693-704.
20. KAYTOR, M. D., and D. M. LIVINGSTON, 1994 Saccharomyces cerevisiae RAD52alleles temperature-sensitive for the repair of DNA double-strand breaks. Genetics 137: 933-944.
21. KAYTOR, M. D., and D. M. LIVINGSTON, 1996 Allele-specific suppression of temperature-sensitive mutations of the Saccharomyces cerevisiae RAD52 gene. Curr. Genet. 29: 203-210.
22. KAYTOR, M. D., M. NGUYEN and D. M. LIVINGSTON, 1995 The complexity of the interaction between RAD52 and SRS2. Genetics 140: 1441-1442.
23. KLEIN, H. L., 1995 Genetic control of intrachromosomal recombination. Bioessays 17: 147-159.
24. KLEIN, H. L., 1997 RDH54, a RAD54 homologue in Saccharomyces cerevisiae, is required for mitotic diploid-specific recombination and repair and for meiosis. Genetics 147: 1533-1543.
25. MALKOVA, A., E. L. IVANOV and J. E. HABER, 1996 Double-strand break repair in the absence of RAD51 in yeast: a possible role for break-induced DNA replication. Proc. Natl. Acad. Sci. USA 93: 7131-7136.
26. MALONE, R. E., B. A. MONTELONE, C. EDWARDS, K. CARNEY and M. F. HOEKSTRA, 1988 A reexamination of the role of the RAD52 gene in spontaneous mitotic recombination. Curr. Genet. 14: 211-223.
27. MILNE, G. T., and D. T. WEAVER, 1993 Dominant negative alleles of RAD52reveal a DNA repair/recombination complex including Rad51 and Rad52. Genes Dev. 7: 1755-1765.
28. MOREAU, S., J. R. FERGUSON and L. S. SYMINGTON, 1999 The nuclease activity of Mre11 is required for meiosis but not for mating type switching, end joining, or telomere maintenance. Mol. Cell. Biol. 19: 556-566.
29. MORTENSEN, U. H., C. BENDIXEN, I. SUSJEVARIC and R. ROTHSTEIN, 1996 DNA strand annealing is promoted by the yeast Rad52 protein. Proc. Natl. Acad. Sci. USA 93: 10729-10734.
30. MORTIMER, R. K., R. CONTOPOULOU and D. SCHILD, 1981 Mitotic chromosome loss in a radiation-sensitive strain of the yeast Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 78: 5778-5782.
31. MURIS, D. F., O. BEZZUBOVA, J. M. BUERSTEDDE, K. VREEKEN, A. S. BALAJEE et al., 1994 Cloning of human and mouse genes homologous to RAD52, a yeast gene involved in DNA repair and recombination. Mutat. Res. 315: 295-305.
32. NEW, J. H., T. SUGIYAMA, E. ZAITSEVA and S. C. KOWALCZYKOWSKI, 1998 Rad52 protein stimulates DNA strand exchange by Rad51 and replication protein A. Nature 391: 407-410.
33. NGUYEN, M. M., and D. M. LIVINGSTON, 1997 Cold-sensitive rad52 alleles of yeast. Curr. Genet. 32: 100-107.
34. NICKOLOFF, J. A., E. Y. CHEN and F. HEFFRON, 1986 A 24-base-pair DNA sequence from the MATlocus stimulates intergenic recombination in yeast. Proc. Natl. Acad. Sci. USA 83: 7831-7835.
35. OGAWA, T., A. SHINOHARA, A. NABETANI, T. IKEYA, X. YU et al., 1993 RecA-like recombination proteins in eukaryotes: functions and structures of RAD51 genes. Cold Spring Harb. Symp. Quant. Biol. 58: 567-576.
36. OSTERMANN, K., A. LORENTZ and H. SCHMIDT, 1993 The fission yeast rad22 gene, having a function in mating-type switching and repair of DNA damages, encodes a protein homolog to Rad52 of Saccharomyces cerevisiae. Nucleic Acids Res. 21: 5940-5944.
37. OZENBERGER, B. A., and G. S. ROEDER, 1991 A unique pathway of double-strand break repair operates in tandemly repeated genes. Mol. Cell. Biol. 11: 1222-1231.
38. PARK, M. S., D. L. LUDWIG, E. STIGGER and S. H. LEE, 1996 Physical interaction between human RAD52 and RPA is required for homologous recombination in mammalian cells. J. Biol. Chem. 271: 18996-19000.
39. PRADO, F., and A. AGUILERA, 1995 Role of reciprocal exchange, one-ended invasion crossover and single-strand annealing on inverted and direct repeat recombination in yeast: different requirements for the RAD1, RAD10, and RAD52 genes. Genetics 139: 109-123.
40. PRAKASH, S., L. PRAKASH, W. BURKE and B. MONTELONE, 1980 Effects of the RAD52 gene on recombination in Saccharomyces cerevisiae. Genetics 94: 31-50.
41. RATTRAY, A. J., and L. S. SYMINGTON, 1994 Use of a chromosomal inverted repeat to demonstrate that the RAD51 and RAD52 genes of Saccharomyces cerevisiae have different roles in mitotic recombination. Genetics 138: 587-595.
42. RATTRAY, A. J., and L. S. SYMINGTON, 1995 Multiple pathways for homologous recombination in Saccharomyces cerevisiae. Genetics 139: 45-56.
43. RESNICK, M. A., 1969 Genetic control of radiation sensitivity in Saccharomyces cerevisiae. Genetics 62: 519-531.
44. RESNICK, M. A., and P. MARTIN, 1976 The repair of double-strand breaks in the nuclear DNA of Saccharomyces cerevisiae and its genetic control. Mol. Gen. Genet. 143: 119-129.
45. RESNICK, M. A., J. NITISS, C. EDWARDS and R. E. MALONE, 1986 Meiosis can induce recombination in rad52 mutants of Saccharomyces cerevisiae. Genetics 113: 531-550.
46. RIJKERS, T., J. VAN DEN OUWELAND, B. MOROLLI, A. G. ROLINK, W. M. BAARENDS et al., 1998 Targeted inactivation of mouse RAd52 reduces homologous recombination but not resistance to ionizing radiation. Mol. Cell. Biol. 18: 6423-6429.
47. SCHILD, D., 1995 Suppression of a new allele of the yeast RAD52 gene by overexpression of RAD51, mutations in srs2 and ccr4, or mating-type heterozygosity. Genetics 140: 115-127.
48. SCHWACHA, A., and N. KLECKNER, 1997 Interhomolog bias during meiotic recombination: meiotic functions promote a highly differentiated interhomolog-only pathway. Cell 90: 1123-1135.
49. SHERMAN, F., G. FINK and J. HICKS, 1986 Methods in Yeast Genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
50. SHINOHARA, A., and T. OGAWA, 1998 Stimulation by Rad52 of yeast Rad51-mediated recombination. Nature 391: 404-407.
51. SHINOHARA, A., H. OGAWA and T. OGAWA, 1992 Rad51 protein involved in repair and recombination in S. cerevisiae is a RecA-like protein. Cell 69: 457-470.
52. SHINOHARA, A., S. GASIOR, T. OGAWA, N. KLECKNER and D. K. BISHOP, 1997a Saccharomyces cerevisiae recA homologues RAD51 and DMC1 have both distinct and overlapping roles in meiotic recombination. Genes Cells 2: 615-629.
53. SHINOHARA, M., E. SHITA-YAMAGUCHI, J. M. BUERSTEDDE, H. SHINAGAWA, H. OGAWA et al., 1997b Characterisation of the roles of the Saccharomyces cerevisiae RAD54 gene and a homologue of RAD54, RDH54/TIDI, in mitosis and meiosis. Genetics 147: 1545-1556.
54. SHINOHARA, A., M. SHINOHARA, T. OHTA, S. MATSUDA and T. OGAWA, 1998 Rad52 forms ring structures and co-operates with RPA in single-strand DNA annealing. Genes Cells 3: 145-156.
55. SMITH, J., and R. ROTHSTEIN, 1995 A mutation in the gene encoding the Saccharomyces cerevisiae single-stranded DNA-binding protein Rfa1 stimulates a RAD2-independent pathway for direct-repeat recombination. Mol. Cell. Biol. 15: 1632-1641.
56. SMITH, J., and R. ROTHSTEIN, 1999 An allele of RFA1 suppresses RAD52-dependent double-strand break repair in Saccharomyces cerevisiae. Genetics 151: 447-458.
57. SUGAWARA, N., and J. E. HABER, 1992 Characterization of double-strand break-induced recombination: homology requirements and single-stranded DNA formation. Mol. Cell. Biol. 12: 563-575.
58. SUGAWARA, N., E. L. IVANOV, J. FISHMAN-LOBELL, B. L. RAY, X. WU et al., 1995 DNA structure-dependent requirements for yeast RAD genes in gene conversion. Nature 373: 84-86.
59. SUGIYAMA, T., J. H. NEW and S. C. KOWALCZYKOWSKI, 1998 DNA annealing by RAD52 protein is stimulated by specific interaction with the complex of replication protein A and single-stranded DNA. Proc. Natl. Acad. Sci. USA 95: 6049-6054.
60. SUNG, P., 1994 Catalysis of ATP-dependent homologous DNA pairing and strand exchange by yeast RAD51 protein. Science 265: 1241-1243.
61. SUNG, P., 1997 Function of yeast Rad52 protein as a mediator between replication protein A and the Rad51 recombinase. J. Biol. Chem. 272: 28194-28197.
62. SUNG, P., and D. L. ROBBERSON, 1995 DNA strand exchange mediated by a RAD51-ssDNA nucleoprotein filament with polarity opposite to that of RecA. Cell 82: 453-461.
63. SYMINGTON, L. S., 1998 Homologous recombination is required for the viability of rad27 mutants. Nucleic Acids Res. 26: 5589-5595.
64. THOMAS, B. J., and R. ROTHSTEIN, 1989 The genetic control of direct-repeat recombination in Saccharomyccs: the effect of rad52 and rad1 on mitotic recombination at GAL10, a transcriptionaily regulated gene. Genetics 123: 725-738.
65. WHITE, C. I., and J. E. HABER, 1990 Intermediates of recombination during mating type switching in Saccharomyces cerevisiae. EMBO J. 9: 663-673.
66. WILLIS, K. K., and H. L. KLEIN, 1987 Intrachromosomal recombination in Saccharomyces cerevisae: reciprocal exchange in an inverted repeat and associated gene conversion. Genetics 117: 633-643.
67. YAMAGUCHI-IWAI, Y., E. SONODA, J. M. BUERSTEDDE, O. BEZZUBOVA, C. MORRISON et al., 1998 Homologous recombination, but not DNA repair, is reduced in vertebrate cells deficient in RAD52. Mol. Cell. Biol. 18: 6430-6435.
68. ZOU, H., and R. ROTHSTEIN, 1997 Holliday junctions accumulate in replication mutants via a RecA homolog-independent mechanism. Cell 90: 87-96.