A cytoplasmic inhibitor of the JNK signal transduction pathway

Science

Volumn 277, Issue 5326, 1997, Pages 693-696

  Dickens, Martin ^a   Rogers, Jeffrey S ^a   Cavanagh, Julie ^a   Raitano, Art ^b   Xia, Zhengui ^c   Halpern, Jocelyn R ^b   Greenberg, Michael E ^c   Sawyers, Charles L ^b   Davis, Roger J ^a  

^a UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MITOGEN ACTIVATED PROTEIN KINASE;

ANIMAL TISSUE; ARTICLE; CELL GROWTH; CONTROLLED STUDY; ENZYME INHIBITION; MOLECULAR INTERACTION; MOUSE; NONHUMAN; PRIORITY JOURNAL; SIGNAL TRANSDUCTION;

ACTIVATING TRANSCRIPTION FACTOR 2; ANIMALS; CA(2+)-CALMODULIN DEPENDENT PROTEIN KINASE; CARRIER PROTEINS; CELL NUCLEUS; CELL TRANSFORMATION, NEOPLASTIC; CELLS, CULTURED; CLONING, MOLECULAR; COS CELLS; CYCLIC AMP RESPONSE ELEMENT-BINDING PROTEIN; CYTOPLASM; FUSION PROTEINS, BCR-ABL; GENE EXPRESSION REGULATION; JNK MITOGEN-ACTIVATED PROTEIN KINASES; MITOGEN-ACTIVATED PROTEIN KINASE 9; MITOGEN-ACTIVATED PROTEIN KINASES; MOLECULAR SEQUENCE DATA; PHOSPHORYLATION; PROTEIN KINASES; PROTO-ONCOGENE PROTEINS C-JUN; RECOMBINANT FUSION PROTEINS; SIGNAL TRANSDUCTION; TRANS-ACTIVATION (GENETICS); TRANSCRIPTION FACTORS; TRANSFECTION;

ANIMALIA; MURINAE;

EID: 0030826412     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.277.5326.693     Document Type: Article

References (40)

1. A. J. Whitmarsh and R. J. Davis, J. Mol. Med. 74, 589 (1996).
2. H. K. Sluss, Z. Han, T. Barrett, R. J. Davis, T. Ip, Genes Dev. 10, 2745 (1996); J. R. Riesgo-Escovar, M. Jenni, A. Fritz, E. Hafen, ibid., p. 2759.
3. H. K. Sluss, Z. Han, T. Barrett, R. J. Davis, T. Ip, Genes Dev. 10, 2745 (1996); J. R. Riesgo-Escovar, M. Jenni, A. Fritz, E. Hafen, ibid., p. 2759.
4. B. Su et al., Cell 77, 727 (1994); M. Rincón, B. Dérijard, C.-W. Chow, R. J. Davis, R. A. Flavell, Genes Funct. 1, 51 (1997).
5. B. Su et al., Cell 77, 727 (1994); M. Rincón, B. Dérijard, C.-W. Chow, R. J. Davis, R. A. Flavell, Genes Funct. 1, 51 (1997).
6. X. Xu et al., Oncogene 13, 153 (1996).
7. A. B. Raitano, J. R. Halpern, T. M. Hambuch, C. L. Sawyers, Proc. Natl. Acad. Sci. U.S.A. 92, 11746 (1995).
8. Z. Xia, M. Dickens, J. Raingeaud, R. J. Davis, M. E. Greenberg, Science 270, 1326 (1995).
9. B. W. Zanke et al., Curr. Biol. 6, 606 (1996); M. Verheij et al., Nature 380, 75 (1996); Y.-R. Chen, C. F. Meyer, T.-H. Tan, J. Biol. Chem. 271, 631 (1996).
10. B. W. Zanke et al., Curr. Biol. 6, 606 (1996); M. Verheij et al., Nature 380, 75 (1996); Y.-R. Chen, C. F. Meyer, T.-H. Tan, J. Biol. Chem. 271, 631 (1996).
11. B. W. Zanke et al., Curr. Biol. 6, 606 (1996); M. Verheij et al., Nature 380, 75 (1996); Y.-R. Chen, C. F. Meyer, T.-H. Tan, J. Biol. Chem. 271, 631 (1996).
12. M. Cavigelli, F. Dolfi, F.-X. Claret, M. Karin, EMBO J. 14, 5957 (1995).
13. I. M. Verma, J. K. Stevenson, E. M. Schwarz, D. Van Antwerp, S. Miyamoto, Genes Dev. 9, 2723 (1995); R. B. McNeill and R. J. Colbran, J. Biol. Chem. 270, 10043 (1995); M. C. Faux and J. D. Scott, Trends Biochem. Sci. 21, 312 (1996).
14. I. M. Verma, J. K. Stevenson, E. M. Schwarz, D. Van Antwerp, S. Miyamoto, Genes Dev. 9, 2723 (1995); R. B. McNeill and R. J. Colbran, J. Biol. Chem. 270, 10043 (1995); M. C. Faux and J. D. Scott, Trends Biochem. Sci. 21, 312 (1996).
15. I. M. Verma, J. K. Stevenson, E. M. Schwarz, D. Van Antwerp, S. Miyamoto, Genes Dev. 9, 2723 (1995); R. B. McNeill and R. J. Colbran, J. Biol. Chem. 270, 10043 (1995); M. C. Faux and J. D. Scott, Trends Biochem. Sci. 21, 312 (1996).
16. P. Cohen, Annu. Rev. Biochem. 58, 453 (1989); M. J. Hubbard and P. Cohen, Trends Biochem. Sci. 18, 172 (1993); J. Inglese, N. J. Freedman, W. J. Koch, R. J. Lefkowitz, J. Biol. Chem. 268, 23735 (1993); V. M. Coghlan et al., Science 267, 108 (1995); D. Mochly-Rosen, ibid. 268, 247 (1995); F. Shibasaki, E. R. Price, D. Lian, F. McKeon, Nature 382, 370 (1996).
17. P. Cohen, Annu. Rev. Biochem. 58, 453 (1989); M. J. Hubbard and P. Cohen, Trends Biochem. Sci. 18, 172 (1993); J. Inglese, N. J. Freedman, W. J. Koch, R. J. Lefkowitz, J. Biol. Chem. 268, 23735 (1993); V. M. Coghlan et al., Science 267, 108 (1995); D. Mochly-Rosen, ibid. 268, 247 (1995); F. Shibasaki, E. R. Price, D. Lian, F. McKeon, Nature 382, 370 (1996).
18. P. Cohen, Annu. Rev. Biochem. 58, 453 (1989); M. J. Hubbard and P. Cohen, Trends Biochem. Sci. 18, 172 (1993); J. Inglese, N. J. Freedman, W. J. Koch, R. J. Lefkowitz, J. Biol. Chem. 268, 23735 (1993); V. M. Coghlan et al., Science 267, 108 (1995); D. Mochly-Rosen, ibid. 268, 247 (1995); F. Shibasaki, E. R. Price, D. Lian, F. McKeon, Nature 382, 370 (1996).
19. P. Cohen, Annu. Rev. Biochem. 58, 453 (1989); M. J. Hubbard and P. Cohen, Trends Biochem. Sci. 18, 172 (1993); J. Inglese, N. J. Freedman, W. J. Koch, R. J. Lefkowitz, J. Biol. Chem. 268, 23735 (1993); V. M. Coghlan et al., Science 267, 108 (1995); D. Mochly-Rosen, ibid. 268, 247 (1995); F. Shibasaki, E. R. Price, D. Lian, F. McKeon, Nature 382, 370 (1996).
20. P. Cohen, Annu. Rev. Biochem. 58, 453 (1989); M. J. Hubbard and P. Cohen, Trends Biochem. Sci. 18, 172 (1993); J. Inglese, N. J. Freedman, W. J. Koch, R. J. Lefkowitz, J. Biol. Chem. 268, 23735 (1993); V. M. Coghlan et al., Science 267, 108 (1995); D. Mochly-Rosen, ibid. 268, 247 (1995); F. Shibasaki, E. R. Price, D. Lian, F. McKeon, Nature 382, 370 (1996).
21. P. Cohen, Annu. Rev. Biochem. 58, 453 (1989); M. J. Hubbard and P. Cohen, Trends Biochem. Sci. 18, 172 (1993); J. Inglese, N. J. Freedman, W. J. Koch, R. J. Lefkowitz, J. Biol. Chem. 268, 23735 (1993); V. M. Coghlan et al., Science 267, 108 (1995); D. Mochly-Rosen, ibid. 268, 247 (1995); F. Shibasaki, E. R. Price, D. Lian, F. McKeon, Nature 382, 370 (1996).
22. K.-Y. Choi, B. Satterberg, D. M. Lyons, E. A. Elion, Cell 78, 499 (1994); T. M. Klauck et al., Science 271, 1589 (1996); M. C. Faux and J. D. Scott, Cell 85, 9 (1996).
23. K.-Y. Choi, B. Satterberg, D. M. Lyons, E. A. Elion, Cell 78, 499 (1994); T. M. Klauck et al., Science 271, 1589 (1996); M. C. Faux and J. D. Scott, Cell 85, 9 (1996).
24. K.-Y. Choi, B. Satterberg, D. M. Lyons, E. A. Elion, Cell 78, 499 (1994); T. M. Klauck et al., Science 271, 1589 (1996); M. C. Faux and J. D. Scott, Cell 85, 9 (1996).
25. A JIP-1 cDNA fragment was isolated by a two-hybrid screen of a mouse embryo cDNA library in the yeast strain L40 [Z. Galcheva-Gargova et al., Science 272, 1797 (1996)]. The bait plasmid (pLexA-JNK1) was constructed by the insertion of JNK1 in the polylinker of pBTM116. Full-length JIP-1 clones were obtained from a mouse brain λZAPII cDNA library (Stratagene), and expression vectors were constructed with the vectors pCMV5, pCDNA3 (Invitrogen), and pGEX-3X (Pharmacia LKB Biotechnology). Mutations were constructed with the polymerase chain reaction. The sequence of the JIP-1 cDNA has been deposited in GenBank with the accession number AF003115.
26. M. Dickens, J. Rogers, J. Cavanagh, Z. Xia, unpublished data.
27. S. Gupta et al., EMBO J. 15, 2760 (1996).
28. B. Dérijard et al., Cell 76, 1025 (1994); H. K. Sluss, T. Barrett, B. Dérijard, R. J. Davis, Mol. Cell. Biol. 14, 8376 (1994); T. Kallunki et al., Genes Dev. 8, 2996 (1994); T. Dai et al., Oncogene 10, 849 (1995).
29. B. Dérijard et al., Cell 76, 1025 (1994); H. K. Sluss, T. Barrett, B. Dérijard, R. J. Davis, Mol. Cell. Biol. 14, 8376 (1994); T. Kallunki et al., Genes Dev. 8, 2996 (1994); T. Dai et al., Oncogene 10, 849 (1995).
30. B. Dérijard et al., Cell 76, 1025 (1994); H. K. Sluss, T. Barrett, B. Dérijard, R. J. Davis, Mol. Cell. Biol. 14, 8376 (1994); T. Kallunki et al., Genes Dev. 8, 2996 (1994); T. Dai et al., Oncogene 10, 849 (1995).
31. B. Dérijard et al., Cell 76, 1025 (1994); H. K. Sluss, T. Barrett, B. Dérijard, R. J. Davis, Mol. Cell. Biol. 14, 8376 (1994); T. Kallunki et al., Genes Dev. 8, 2996 (1994); T. Dai et al., Oncogene 10, 849 (1995).
32. R. Marais, J. Wynne, R. Treisman, Cell 73, 381 (1993); A. J. Whitmarsh, P. Shore, A. D. Sharrocks, R. J. Davis, Science 269, 403 (1995); H. Gille, T. Strahl, P. E. Shaw, Curr. Biol. 5, 1191 (1995); J. Raingeaud, A. J. Whitmarsh, T. Barrett, B. Dérijard, R. J. Davis, Mol. Cell. Biol. 16, 1247 (1996).
33. R. Marais, J. Wynne, R. Treisman, Cell 73, 381 (1993); A. J. Whitmarsh, P. Shore, A. D. Sharrocks, R. J. Davis, Science 269, 403 (1995); H. Gille, T. Strahl, P. E. Shaw, Curr. Biol. 5, 1191 (1995); J. Raingeaud, A. J. Whitmarsh, T. Barrett, B. Dérijard, R. J. Davis, Mol. Cell. Biol. 16, 1247 (1996).
34. R. Marais, J. Wynne, R. Treisman, Cell 73, 381 (1993); A. J. Whitmarsh, P. Shore, A. D. Sharrocks, R. J. Davis, Science 269, 403 (1995); H. Gille, T. Strahl, P. E. Shaw, Curr. Biol. 5, 1191 (1995); J. Raingeaud, A. J. Whitmarsh, T. Barrett, B. Dérijard, R. J. Davis, Mol. Cell. Biol. 16, 1247 (1996).
35. R. Marais, J. Wynne, R. Treisman, Cell 73, 381 (1993); A. J. Whitmarsh, P. Shore, A. D. Sharrocks, R. J. Davis, Science 269, 403 (1995); H. Gille, T. Strahl, P. E. Shaw, Curr. Biol. 5, 1191 (1995); J. Raingeaud, A. J. Whitmarsh, T. Barrett, B. Dérijard, R. J. Davis, Mol. Cell. Biol. 16, 1247 (1996).
36. J. Raingeaud et al., J. Biol. Chem. 270, 7420 (1995).
37. Single-letter abbreviations for the amino acid residues are as follows: A, Ala; G, Gly; K, Lys; L, Leu; S, Ser; and T, Thr.
38. Luciferase reporter assays in CHO cells were performed 48 hours after transfection with the use of β-galactosidase (β-Gal) to measure transfection efficiency (14). The cells were activated by treatment with fetal calf serum (10%). The data are presented as the relative luciferase activity [mean ± SEM of the activity ratio of luciferase/β-Gal (n = 3)].
39. We performed bone marrow transformation assays using recombinant retroviruses [C. L. Sawyers, J. McLaughlin, O. N. Witte, J. Exp. Med. 181, 307 (1995)] that were packaged with 293T cells. We constructed the bi-cistronic retroviruses expressing the JBD and Bcr-Abl by subcloning p185Bcr-Abl in the Cla I site and the JBD in the Eco RI site of pSRαTK.
40. We thank J. Cooper and S. Hollenberg for two-hybrid reagents; D. Schmidt and F. Fay for assistance with immunofluorescence microscopy; M. Wysk, R. Tizard, and J. Demarinis for Maxam-Gilbert sequencing; I.-H. Wu for technical assistance; T. Barrett for automated DNA sequencing; and K. Gemme for secretarial assistance. Supported by NIH grants CA43855 (M.E.G.) and CA65861 (R.J.D.) and by a grant from the James S. McDonnell Foundation (C.L.S.). R.J.D. is an investigator of the Howard Hughes Medical Institute. C.L.S. is a scholar of the Leukemia Society of America.