Specific binding to a novel and essential Golgi membrane protein (Yip1p) functionally links the transport GTPases Ypt1p and Ypt31p

EMBO Journal

Volumn 17, Issue 17, 1998, Pages 4954-4963

  Yang, Xiaoping ^a   Matern, Hugo T ^a   Gallwitz, Dieter ^a  

^a MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

Author keywords

Golgi; Rab GTPase; Secretion; Two hybrid system; Vesicular protein transport; Ypt

Indexed keywords

GUANOSINE TRIPHOSPHATASE; MEMBRANE PROTEIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; CELL FRACTIONATION; CELL MUTANT; CELL VIABILITY; CONTROLLED STUDY; ENDOPLASMIC RETICULUM; GOLGI COMPLEX; IMMUNOFLUORESCENCE; IMMUNOPRECIPITATION; MEMBRANE FUSION; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN GLYCOSYLATION; PROTEIN PROTEIN INTERACTION; PROTEIN SECRETION; STEADY STATE;

AMINO ACID SEQUENCE; BIOLOGICAL TRANSPORT; CELL COMPARTMENTATION; GLYCOSYLATION; GOLGI APPARATUS; GTP PHOSPHOHYDROLASES; GTP-BINDING PROTEINS; MOLECULAR SEQUENCE DATA; PROTEIN BINDING; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEINS; RAB GTP-BINDING PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SUBCELLULAR FRACTIONS;

EUKARYOTA; MAMMALIA;

EID: 0032168280     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/17.17.4954     Document Type: Article

References (52)

1. Bacon, R.A., Salminen, A., Ruohola, H., Novick, P. and Ferro-Novick, S. (1989) The GTP-binding protein Ypt1 is required for transport in vitro - the Golgi apparatus is detective in ypt1 mutants. J. Cell Biol., 109, 1015-1022.
2. Becker, J., Tan, T.J., Trepte, H.H. and Gallwitz, D. (1991) Mutational analysis of the putative effector domain of the GTP-binding Ypt1 protein in yeast suggests specific regulation by a novel GAP activity. EMBO J., 10, 785-792.
3. Benli, M., Döring, F., Robinson, D.G., Yang, X. and Gallwitz, D. (1996) Two GTPase isoforms, Ypt31p and Ypt32p, are essential for Golgi function in yeast. EMBO J., 15, 6460-6475.
4. Bennett, M.K. and Scheller, R.H. (1994) A molecular description of synaptic vesicle membrane trafficking. Annu. Rev. Biochem., 63, 63-100.
5. Chavrier, P., Gorvel, J.P., Stelzer, E., Simons, K., Gruenberg, J. and Zerial, M. (1991) Hypervariable C-terminal domain of rab proteins acts as a targeting signal. Nature. 353, 769-772.
6. Dascher, C. Ossig, R., Gallwitz, D. and Schmitt, H.D. (1991) Identification and structure of four yeast genes (5LY) that are able to suppress the functional loss of YPT1, a member of the ras-superfamily. Mol. Cell. Biol., 11, 872-885.
7. Diaz, E., Schimmoller, F. and Pfeffer, S.R. (1997) A novel Rab9 effector required for endosome-to-TGN transport. J. Cell Biol., 138, 283-290.
8. Durfee, T., Becherer, K., Chen, P.L., Yeh, H., Yang, Y., Kilburn, A.E., Lee, W.H. and Elledge, S.J. (1993) The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit. Genes Dev., 7, 555-569.
9. Echard, A., Jollivet, F., Martinez, O., Lacapere, J.J. and Goud, B. (1998) Interaction of a Golgi-associated kinesin-like protein with Rab6. Science, 279, 580-585.
10. Fields, S. and Song, O. (1989) A novel genetic system to detect protein-protein interactions. Nature, 340, 245-246.
11. Fromant, M., Blanquet, S. and Plateau, P. (1995) Direct random mutagenesis of gene-sized DNA fragments using polymerase chain reaction. Anal. Biochein., 224, 347-53.
12. Garrett, M.D., Zahner, J.E., Cheney, C.M. and Novick, P.J. (1994) GD11 encodes a GDP dissociation inhibitor that plays an essential role in the yeast secretory pathway. EMBO J., 13, 1718-1728.
13. Grabowski, R. and Gallwitz, D. (1997) High-affinity binding of the yeast cis-Golgi t-SNARE, Sed5p, to wild-type and mutant Sly1p, a modulator of transport vesicle docking. FEBS Lett., 411, 169-72.
14. Graham, T.R. and Emr, S.D. (1991) Compartmental organization of Golgi-specilic protein modification and vacuolar protein sorting events defined in a yeast sec18 (NSF) mutant. J. Cell Biol., 114, 207-218.
15. Haas, A., Scheglmann, D., Lazar, T., Gallwitz, D. and Wickner, W. (1995) The GTPase Ypt7p of Saccharomyces cerevisiae is required on both partner vacuoles for the homotypic fusion step of vacuole inheritance. EMBO J., 14, 5258-5270.
16. Hardwick, K.G. and Pelham, H.R.B. (1992) SED5 encodes a 39-kD integral membrane protein required for vesicular transport between the ER and the Golgi complex. J. Cell Biol., 119, 513-521.
17. Harlow, E. and Lane, O. (1988) Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
18. Harper, J.W., Adami, G.R., Wei, N., Keyomarsi, K. and Elledge, S.J. (1993) The p21 Cdk-interacting protein Cip1 is a potential inhibitor of G1 cyclin-dependent kinases. Cell, 75, 805-816.
19. Haucke, V. and Schatz, G. (1997) Reconstitution of the protein insertion machinery of the mitochondrial inner membrane. EMBO J., 16, 4560-4567.
20. Jedd, G., Richardson, C., Litt, R. and Segev, N. (1995) The Ypt1 GTPase is essential for the first two steps of the yeast secretory pathway. J. Cell Biol., 131, 583-590.
21. Jedd, G., Mulholland, J. and Segev, N. (1997) Two new Ypt GTPases are required for exit from the yeast trans-Golgi compartment. J. Cell Biol., 137, 563-580.
22. Kunkel, T.A., Roberts, J.D. and Zakour, R.A. (1987) Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol., 154, 367-382.
23. Lazar, T., Goette, M. and Gallwitz, D. (1997) Vesicular transport: how many Ypt/Rab-GTPases make an eukaryotic cell? Trends Biochem. Sci., 22, 468-472.
24. Lian, J.P., Stone, S., Jiang, Y., Lyons, P. and Ferro-Novick, S. (1994) Ypt1p implicated in v-SNARE activation. Nature, 372, 698-701.
25. Li, B. and Warner, J.R. (1996) Mutation of the Rab6 homologue of Sacharomyces cerevisiae, YPT6, inhibits both early Golgi function and ribosome biosynthesis. J. Biol. Chem., 271, 16813-16819.
26. Lupashin, V.V. and Waters, M.G. (1997) t-SNARE activation through transient interaction with a Rab-like guanosine triphosphate. Science, 276, 1255-1258.
27. Machamer, C.E., Grim, M.G., Esquela, A., Chung, S.W., Rolls, M., Ryan, K. and Swift, A.M. (1993) Retention of a cis Golgi protein requires polar residues on one face of a predicted α-helix in the transmembrane domain. Mol. Biol. Cell, 4, 695-704.
28. Mayer, A. and Wickner, W. (1997) Docking of yeast vacuoles is catalyzed by the Ras-like GTPase Ypt7p after symmetric priming by Sec18p (NSF). J. Cell Biol., 136, 307-318.
29. Mitchell, D., Marshall, T.A. and Deschenes, R.J. (1993) Vectors for the inducible overexpression of gluthathione S-transferase fusion proteins in yeast. Yeast, 9, 715-723.
30. Munro, S. (1991) Sequences within and adjacent to the transmembrane segment of the α-2,6-sialyltransferase specify Folgi retention. EMBO J., 10, 3577-3588.
31. Novick, P. and Zerial, M. (1997) The diversity of Rab proteins in vesicle transport. Curr. Opin. Cell Biol., 9, 496-504.
32. Novick, P., Ferro, S. and Schekman, R. (1981) Order of events in the yeast secretory pathway. Cell, 25, 461-469.
33. Pfeffer, S.R. (1996) Transport vesicle docking: SNAREs and associates. Annu. Rev. Cell Dev. Biol., 12, 441-461.
34. Ren, M., Zeug, J., De Lemos-Chiarandini, C., Rosenfeld, M., Adesnik, M. and Sabatini, D.D. (1996) In its active form, the GTP-binding protein Rab8 interacts with a stress-activated protein kinase. Proc. Natl Acad. Sci. USA, 93, 5151-5155.
35. Rexach, M.F. and Schekman, R.W. (1991) Distinct biochemical requirements for the budding, targeting, and fusion of ER-derived transport vesicles. J. Cell Biol., 114, 219-229.
36. Rothman, J.E. and Wieland, F.T. (1996) Protein sorting by transport vesicles. Science, 272, 227-234.
37. Salminen, A. and Novick, P. (1987) DNA sequencing with chain terminating inhibitors. Proc. Natl Acad. Sci. USA, 74, 5463-5467.
38. Schmitt, H.D., Wagner, P. Pfaff, E. and Gallwitz, D. (1986) The ras-related YPT1 gene product in yeast: a GTP-binding protein that might be involved in microtubule organization. Cell, 47, 401-412.
39. Schmitt, H.D., Puzicha, M. and Gallwitz, D. (1988) Study of a temperature-sensitive mutant of the ras-related YPT1 gene product in yeast suggests a role in the regulation of intracellular calcium. Cell, 53, 635-647.
40. Schröder, S., Schimmöller, F., Singer-Krüger, B. and Riezman, H. (1995) The Golgi-localization of yeast Emp47p depends on its di-lysine motif but is not affected by the ret1-1 mutation in alpha-COP. J. Cell Biol., 131, 895-912.
41. Segev, N., Mulholland, J. and Botstein, D. (1988) The yeast GTP-binding Ypt1 protein and a mammalian counterpart are associated with the secretion machinery. Cell, 52, 915-924.
42. Sherman, F. Fink, G.R. and Hicks, J.B. (1986) Methods in Yeast Genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
43. Søgaard, M., Ye, K., Tani, R.R., Geromanos, S., Tempst, P., Kirchhausen, T., Rothman, J.E. and Söllner, T. (1994) A rab protein is required for the assembly of SNARE complexes in the docking of transport vesicles. Cell, 78, 937-948.
44. Soldati, T., Shapiro, A.D., Svejstrup, A.B.D. and Pfeffer, S.R. (1994) Membrane targeting of the small GTPase rab9 is accompanied by nucleotide exchange. Nature, 369, 76-78.
45. Stenmark, H., Vitale, G., Ullrich, O. and Zerial, M. (1995) Rabaptin-5 is a direct effector of the small GTPase Rab5 in endocytic membrane fusion. Cell. 83, 423-432.
46. Tsukada, M. and Gallwitz, D. (1996) Isolation and characterization of SYS genes from yeast, multicopy suppressors of the functional loss of the transport GTPase Ypt6p. J. Cell Sci., 109, 2471-2481.
47. Ullrich, O., Horiuchi, H., Bucci, C. and Zerial, M. (1994) Membrane association of rab5 mediated by GDP-dissociation inhibitor and accompanied by GDP/GTP exchange. Nature, 368, 157-160.
48. Ungermann, C., Nichols, B.J., Pelham, H.R.B. and Wickner, W. (1998) A vacuolar v/t-SNARE complex, the predominant form in vivo and on isolated vacuoles, is disassembled and activated for docking and fusion. J. Cell Biol., 140, 61-69.
49. Wagner, P., Molenaar, C.M.T., Rauh, A.J.G., Brökel, R., Schmitt, H.D. and Gallwitz, D. (1987) Biochemical properties of the ras-related YPT protein in yeast: a mutational analysis. EMBO J., 6, 2373-2379.
50. Wagner, P., Hengst, L. and Gallwitz, D. (1992) Ypt-proteins in yeast. Methods Enzymol., 219, 369-387.
51. Walch-Solimena, C., Collins, R.N. and Novick, P.J. (1997) Sec2p mediates nucleotide exchange on sec4p and is involved in polarized delivery of post-Golgi vesicles. J. Cell Biol., 137, 1495-1509.
52. Wichmann, H., Hengst, L. and Gallwitz, D. (1992) Endocytosis in yeast: evidence for the involvement of a small GTP-binding protein (Ypt7p). Cell, 71, 1131-1142.