Sec2p mediates nucleotide exchange on Sec4p and is involved in polarized delivery of post-Golgi vesicles

Journal of Cell Biology

Volumn 137, Issue 7, 1997, Pages 1495-1509

  Walch Solimena, Christiane ^a   Collins, Ruth N ^a   Novick, Peter J ^a  

^a YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GUANOSINE TRIPHOSPHATASE; NUCLEOTIDE;

ARTICLE; CELL FRACTIONATION; CELL POLARITY; CELL VACUOLE; EXOCYTOSIS; GOLGI COMPLEX; IMMUNOBLOTTING; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN INTERACTION; SACCHAROMYCES CEREVISIAE; SECRETORY GRANULE; YEAST;

AMINO ACID SEQUENCE; BIOLOGICAL TRANSPORT; CELL POLARITY; FUNGAL PROTEINS; GENE EXPRESSION REGULATION, FUNGAL; GOLGI APPARATUS; GTP-BINDING PROTEINS; MOLECULAR SEQUENCE DATA; MUTATION; RAB GTP-BINDING PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE ALIGNMENT;

SACCHAROMYCES CEREVISIAE;

EID: 0030930514     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.137.7.1495     Document Type: Article

References (59)

1. Aalto, M.K., H. Ronne, and S. Keränen. 1993. Yeast syntaxins Sso1p and Sso2p belong to a family of related membrane proteins that function in vesicular transport. EMBO (Eur. Mol. Biol. Organ.) J. 12:4095-4104.
2. Altschul, S.F., W. Gish, W. Miller, E.W. Myers, and D.J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215:403-410.
3. Araki, S., K. Kaibuchi, T. Sasaki, Y. Hata, and Y. Takai. 1991. Role of the C-terminal region of smg p25A in its interaction with membranes and the GDP/GTP exchange protein. Mol. Cell. Biol. 11:1438-1447.
4. Bourne, H.R., D.A. Sanders, and F. McCormick. 1990. The GTPase superfamily: a conserved switch for diverse cell functions. Nature (Lond.). 348:125-132.
5. Bowser, R., and P. Novick. 1991. Sec15 protein, an essential component of the exocytotic apparatus, is associated with the plasma membrane and with a soluble 19.5 S particle. J. Cell Biol. 112:1117-1131.
6. Bowser, R., H. Müller, B. Govindan, and P. Novick. 1992. Sec8p and Sec15p are components of a plasma membrane-associated 19.5 S particle. J. Cell Biol. 118:1041-1056.
7. Brennwald, P., and P. Novick. 1993. Interactions of three domains distinguishing the Ras-related GTP-binding proteins Ypt1 and Sec4. Nature (Lond.). 362:560-563.
8. Brennwald, P., B. Kearns, K. Champion, S. Keranen, V. Bankaitis, and P. Novick. 1994. Sec9 is a SNAP-25-like component of a yeast SNARE complex that may be the effector of Sec4 function in exocytosis. Cell. 79:245-258.
9. Brondyk, W.H., C.J. McKiernan, K.A. Fortner, P. Stabila, R.W. Holz, and I.G. Macara. 1995. Interaction cloning of Rabin3, a novel protein that associates with the Ras-like GTPase Rab3A. Mol. Cell. Biol. 15:1137-1143.
10. Chien, C.T., P.L. Bartel, R. Sternglanz, and S. Fields. 1991. The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest. Proc. Natl. Acad. Sci. USA. 88:9578-9582.
11. Collins, R.N., P. Brennwald, M. Garrett, A. Lauring, and P. Novick. 1997. Interactions of nucleotide release factor DSS4 with SEC4 in the post-golgi secretory pathway of yeast. J. Biol. Chem. In press.
12. Drubin, D.G., and W. James Nelson. 1996. Origins of cell polarity. Cell. 84:335-344.
13. 2+)-binding site of RasH prevent its activation by GTP. Mol. Cell. Biol. 11:4822-4829.
14. Ferro-Novick, S., and P. Novick. 1993. The role of GTP-binding proteins in transport along the exocytic pathway. Ann. Rev. Cell Biol. 9:575-599.
15. Garrett, M.D., J.E. Zahner, C.M. Cheney, and P.J. Novick. 1994. GDI1 encodes a GDP dissociation inhibitor that plays an essential role in the yeast secretory pathway. EMBO (Eur. Mol. Biol. Organ.) J. 13:1718-1722.
16. Gietz, R.D.,and R.H. Schiestl. 1991. Applications of high efficiency lithium acetate transformation of intact yeast cells using single-stranded nucleic acids as carrier. Yeast. 7:253-263.
17. Goud, B., A. Salminen, N.C. Walworth, and P.J. Novick. 1988. A GTP-binding protein required for secretion rapidly associates with secretory vesicles and the plasma membrane in yeast. Cell. 53:753-768.
18. Govindan, B., and P. Novick. 1995. Development of cell polarity in budding yeast. J. Exp. Zool. 273:401-424.
19. Govindan, B., R. Bowser, and P. Novick. 1995. The role of Myo2, a yeast class V myosin, in vesicular transport. J. Cell Biol. 128:1055-1068.
20. Janoueix-Lerosey, I., F. Jollivet, J. Camonis, P.N. Marche, and B. Goud. 1995. Two-hybrid system screen with the small GTP-binding protein Rab6. Identification of a novel mouse GDP dissociation inhibitor isoform and two other potential partners of Rab6. J. Biol. Chem. 270:14801-14808.
21. Johnston, G.C., J.A. Prendergast, and R.A. Singer. 1991. The Saccharomyces cerevisiae MYO2 gene encodes an essential myosin for vectorial transport of vesicles. J. Cell Biol. 113:539-551.
22. Kabcenell, A.K., B. Goud, J.K. Northup, and P. Novick. 1990. Binding and hydrolysis of guanine nucleotides by Sec4p, a yeast protein involved in the regulation of vesicular traffic. J. Biol. Chem. 265:9366-9372.
23. Kaiser, C.A., and R. Schekman. 1990. Distinct sets of SEC genes govern transport vesicle formation and fusion early in the secretory pathway. Cell. 61: 723-733.
24. Kurzchalia, T.V., J.P. Gorvel, P. Dupree, R. Parton, R. Kellner, T. Houthaeve, J. Gruenberg, and K. Simons. 1992. Interactions of Rab5 with cytosolic proteins. J. Biol. Chem. 267:18419-18423.
25. 2+/phospholipid-binding protein, depends on rab3A/3C. Neuron. 13:885-898.
26. Lian, J.P., and S. Ferro-Novick. 1993. Bos1p, an integral membrane protein of the endoplasmic reticulum to Golgi transport vesicles, is required for their fusion competence. Cell. 73:735-745.
27. Lian, J.P., S. Stone, Y. Jiang, P. Lyons, and S. Ferro-Novick. 1994. Ypt1p implicated in v-SNARE activation. Nature (Lond.). 372:698-701.
28. Moya, M., D. Roberts, and P. Novick. 1993. DSS4-1 is a dominant suppressor of sec4-8 that encodes a nucleotide exchange protein that aids Sec4p function. Nature (Lond.). 361:460-463.
29. Mulholland, A., D. Preuss, A. Moon, A. Wong, D. Drubin, and D. Botstein. 1994. Ultrastructure of the yeast actin cytoskeleton and its association with the plasma membrane. J. Cell Biol. 125:381-391.
30. Nair, J., H. Müller, M. Peterson, and P. Novick. 1990. Sec2 protein contains a coiled-coil domain essential for vesicular transport and a dispensable carboxy terminal domain. J. Cell Biol. 110:1897-1909.
31. Novick, P.J., C. Field, and R. Schekman. 1980. Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway. Cell. 21:205-215.
32. Novick, P., and D. Botstein. 1985. Phenotypic analysis of temperature-sensitive yeast actin mutants. Cell. 40:405-416.
33. Novick, P., and P. Brennwald. 1993. Friends and family: the role of the Rab GTP-ases in vesicular traffic. Cell. 75:597-601.
34. Novick, P.J., B. Goud, A. Salminen, N.C. Walworth, J. Nair, and M. Potenza. 1988. Regulation of vesicular traffic by a GTP-binding protein on the cytoplasmic surface of secretory vesicles in yeast. Cold Spring Harb. Symp. Quant. Biol. 53:637-467.
35. Peränen, J., P. Auvinen, H. Virta, R. Wepf, and K. Simons. 1996. Rab8 promotes polarized membrane transport through reorganization af actin and microtubules in fibroblasts. J. Cell Biol. 135:153-167.
36. Pfeffer, S.R. 1996. Transport vesicle docking - SNAREs and associates. Ann. Rev. Cell Devel. Biol. 12:441-461.
37. Preuss, D., J. Mulholland, A. Franzusoff, N. Segev, and D. Botstein. 1992. Characterization of the Saccharomyces Golgi complex through the cell cycle by immunoelectron microscopy. Mol. Cell. Biol. 3:789-803.
38. Protopopov, V., B. Govindan, P. Novick, and J.E. Gerst. 1993. Homologs of the synaptobrevin/VAMP family of synaptic vesicle proteins function on the late secretory pathway in S. cerevisiae. Cell. 74:855-861.
39. Roberts, D.M., M.D. Garrett, and P. Novick. 1995. Purification of the GDP dissociation stimulator Dss4 from recombinant bacteria. Methods Enzymol. 257:84-92.
40. Rothman, J.E., and G. Warren. 1994. Implications of the SNARE hypothesis for intracellular membrane topology and dynamics. Curr. Biol. 4:220-233.
41. Sambrook, J., E.F. Fritsch, and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
42. Salminen, A., and P.J. Novick. 1989. The Sec15 protein responds to the function of the GTP binding protein. Sec4, to control vesicular traffic in yeast. J. Cell Biol. 109:1023-1036.
43. Shirataki, H., K. Kaibuchi, T. Sakoda, S. Kishida, T. Yamaguchi, K. Wada, M. Miyazaki, and Y. Takai. 1993. Rabphilin-3A. a putative target protein for smg p25A/rab3A p25 small GTP-binding protein related to synaptotagmin. Mol. Cell. Biol. 13:2061-2068.
44. Simon, I., M. Zerial, and R.S. Goody. 1996. Kinetics of interaction of Rab5 and Rab7 wilh nucleotides and magnesium ions. J. Biol. Chem. 271:20470-20478.
45. Søgaard, M., K. Tani, R.R. Ye, S. Geromanos, P. Tempst, T. Kirchhausen, J.E. Rothman, and T. Söllner. 1994. A Rab protein is required for the assembly of SNARE complexes in the docking of transport vesicles. Cell. 78:937-948.
46. Soldati, T., A.D. Shapiro, A.B. Svejstrup, and S.R. Pfeffer. 1994. Membrane targeting of the small GTPase Rab9 is accompanied by nucleotide exchange. Nature (Lond.). 369:76-78.
47. Söllner, T., M. Bennett, S.W. Whiteheart, R.H. Scheller, and J.E. Rothman. 1993. A protein assembly-disassembly pathway in vitro that may correspond to sequential steps of synaptic vesicle docking, activation and fusion. Cell. 75: 409-418.
48. Stahl, B., J.H. Chou, C. Li, T.C. Südhof, and R. Jahn. 1996. Rab3 reversibly recruits rabphilin to synaptic vesicles by a mechanism analogous to raf recruitment by ras. EMBO (Eur. Mol. Biol. Organ.) J. 15:1799-1809.
49. Stenmark, H., R.G. Parton, O. Steele-Mortimer, A. Lutcke, J. Gruenberg, and M. Zerial. 1994. Inhibition of Rab5 GTPase activity stimulates membrane fusion in endocytosis. EMBO (Eur. Mol. Biol. Organ.) J. 13:1287-1296.
50. Stenmark, H., G. Vitale, O. Ullrich, and M. Zerial. 1995. Rabaptin5 is a direct effector of the small GTPase Rab5 in endocytic membrane fusion. Cell. 83: 423-432.
51. TerBush, D.R., and P. Novick. 1995. Sec6, Sec8, and Sec15 are components of a multisubunit complex which localizes to small bud tips in Saccharomyces cerevisiae. J. Cell Biol. 130:299-312.
52. TerBush, D.R., T. Maurice, D. Roth, and P. Novick. 1996. The Exocyst is a multiprotein complex required for exocytosis in Saccharomyces cerevisiae. EMBO (Eur. Mol. Biol. Organ.) J. 15:6483-6494.
53. Ullrich, O., H. Horiuchi, C. Bucci, and M. Zerial. 1994. Membrane association of Rab5 mediated by GDP-dissociation inhibitor and accompanied by GDP/GTP exchange. Nature (Lond.). 368:157-160.
54. Wada, M., H. Nakanishi, A. Satoh, H. Hirano, H. Obaishi, Y. Matsuura, and Y. Takai. 1997. Isolation and characterization of a GDP/GTP exchange protein specific for the Rab3 subfamily of small G proteins. J. Biol. Chem. 272:3875-3878.
55. Walworth, N.C., and P.J. Novick. 1987. Purification and characterization of constitutive secretory vesicles from yeast. J. Cell Biol. 105:163-174.
56. Walworth, N.C., B. Goud, A.K. Kabcenell, and P.J. Novick. 1989. Mutational analysis of SEC4 suggests a cyclical mechanism for the regulation of vesicular traffic. EMBO (Eur. Mol. Biol. Organ.) J. 8:1685-1693.
57. Walworth, N.C., P. Brennwald, A.K. Kabcenell, M. Garrett, and P. Novick. 1992. Hydrolysis of GTP by Sec4 protein plays an important role in vesicular transport and is stimulated by a GTPase-activating protein in Saccharomyces cerevisiae. Mol. Cell. Biol. 12:2017-2028.
58. 2+ binding, and structural characterization of the guanine nucleotide exchange factor human Mss4. Biochemistry. 34:9103-9110.
59. Zerial, M., and H. Stenmark. 1993. Rab GTPases in vesicular transport. Curr. Opin. Cell Biol. 5:613-620.