Two new Ypt GTPases are required for exit from the yeast trans-Golgi compartment

Journal of Cell Biology

Volumn 137, Issue 3, 1997, Pages 563-580

  Jedd, Gregory ^a   Mulholland, Jon ^b   Segev, Nava ^a  

^a UNIVERSITY OF CHICAGO   (United States)

^b STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GUANOSINE TRIPHOSPHATASE;

CELL VACUOLE; ELECTRON MICROSCOPY; EXOCYTOSIS; GOLGI COMPLEX; IMMUNOBLOTTING; PHENOTYPE; PRIORITY JOURNAL; PROTEIN PURIFICATION; PROTEIN TARGETING; PROTEIN TRANSPORT; REVIEW;

CELL COMPARTMENTATION; CELL DIVISION; EXOCYTOSIS; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; FUNGAL PROTEINS; GENE DELETION; GENES, FUNGAL; GOLGI APPARATUS; GTP PHOSPHOHYDROLASES; INTRACELLULAR MEMBRANES; MICROSCOPY, ELECTRON; MUTAGENESIS, SITE-DIRECTED; PRECIPITIN TESTS; SACCHAROMYCES CEREVISIAE; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 0030969912     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.137.3.563     Document Type: Review

References (78)

1. Achstetter, T., A. Franzusoff, C. Field, and R. Schekman. 1988. SEC7 encodes an unusual, high molecular weight protein required for membrane traffic from the yeast Golgi apparatus. J. Biol. Chem. 263:11711-11717.
2. Bacon, R.A., A. Salminen, H. Ruohola, P. Novick, and S. Ferro-Novick. 1989. The GTP-binding protein Ypt1 is required for transport in vitro: the Golgi apparatus is defective in ypt1 mutants. J. Cell Biol. 109:1015-1022.
3. Baker, D., L. Wuestehube, R. Schekman, D. Botstein, and N. Segev. 1990. GTP-binding Ypt1 protein and Ca2+ function independently in a cell-free transport reaction. Proc. Natl. Acad. Sci. USA. 87:355-359.
4. Bankaitis, V.A., D.E. Malehorn, S.D. Emr, and R. Greene. 1989. The Saccharomyces cerevisiae SEC14 gene encodes a cytosolic factor that is required for transport of secretory proteins from the yeast Golgi complex. J. Cell Biol. 108:1271-1281.
5. Banta, L.M., J.S. Robinson, D.J. Klionsky, and S.D. Emr. 1988. Organelle assembly in yeast: Characterization of yeast mutants defective in vacuolar biogenesis and protein sorting. J. Cell Biol. 107:1369-1383.
6. Baudin, A., O. Ozier-Kalogeropoulos, A. Denouel, F. Lacroute, and C. Cullin. 1993. A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae. Nucleic Acids. Res. 21:3329-3330.
7. Bennett, M.K., and R.H. Scheller. 1993. The molecular machinery for secretion is conserved from yeast to neurons. Proc. Natl. Acad. Sci. USA. 90:2559-2563.
8. Brennwald, P., B. Kearns, K. Champion, S. Keranen, V. Bankaitis, and P. Novick. 1994. Sec9 is a SNAP-25-like component of a yeast SNARE complex that may be the effector of Sec4 function in exocytosis. Cell. 79:245-258.
9. Byers, B. 1981. Cytology of the yeast life cycle. In The Molecular Biology of the Yeast Saccharomyces: Life Cycle and Inheritance. J.N. Strathern, E.W. Jones, and R.J. Broach, editors. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. 59-96.
10. Chuang, J.S., and R.W. Schekman. 1996. Differential trafficking and timed localization of two chitin synthase proteins, Chs2p and Chs3p. J. Cell Biol. 135: 597-610.
11. Cunningham, K.W., and W.T. Wickner. 1989. Yeast KEX2 protease and mannosyltransferase I are located to distinct compartments of the secretory pathway. Yeast. 5:25-33.
12. Deretic, D., B. Puleo-Scheppke, and C. Trippe. 1996. Cytoplasmic domain of rhodopsin is essential for post-Golgi vesicle formation in a retinal cell free system. J. Biol. Chem. 271:2279-2286.
13. Ferro-Novick, S., and R. Jahn. 1994. Vesicle fusion from yeast to man. Nature (Lond.). 370:191-193.
14. Ferro-Novick, S., and P. Novick. 1993. The role of GTP-binding proteins in transport along the exocytic pathway. Annu. Rev. Cell Biol. 9:575-599.
15. Franzusoff, A., and R. Schekman. 1989. Functional compartments of the yeast Golgi apparatus are defined by the sec7 mutation. EMBO (Eur. Mol. Biol. Organ.) J. 8:2695-2702.
16. Franzusoff, A., K. Redding, J. Crosby, S.R. Fuller, and R. Schekman. 1989. Localization of components involved in protein transport and processing through the yeast Golgi apparatus. J. Cell Biol. 112:27-37.
17. Futter, C.E., C.N. Connolly, D.F. Cutler, and C.R. Hopkins. 1995. Newly synthesized transferrin receptors can be detected in the endosome before they appear on the cell surface. J. Biol. Chem. 270:10999-11003.
18. Goud, B., A. Salminen, N.C. Walworth, and P. Novick. 1988. A GTP-binding protein required for secretion rapidly associates with secretory vesicles and the plasma membrane in yeast. Cell. 53:753-768.
19. Goud, B., A. Zahraoui, A. Tavitian, and J. Saraste. 1990. Small GTP-binding protein associated with Golgi cisternae. Nature (Lond.). 345:553-556.
20. Graham, T.R., and S.D. Emr. 1991. Compartmental organization of Golgi-specific protein modification and vacuolar protein sorting events defined in a yeast sec18 (NSF) mutant. J. Cell Biol. 114:207-218.
21. Guan, K.L., and J.E. Dixon. 1991. Eukaryotic proteins expressed in Escherichia coli: an improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase. Anal. Biochem. 192:262-267.
22. Harsay, E., and A. Bretscher. 1995. Parallel secretory pathways to the cell surface in yeast. J. Cell Biol. 131:297-310.
23. Hicke, L., and H. Riezman. 1996. Ubiquitination of a yeast plasma membrane receptor signals its ligand stimulated endocytosis. Cell. 84:277-287.
24. Horazdovsky, B., and S. Emr. 1993. The VPS16 gene product associates with a sedimentable protein complex and is essential for vacuolar protein sorting in yeast. J. Biol. Chem. 268:4953-4962.
25. Horazdovsky, F.B., R.G. Busch, and S.D. Emr. 1994. VPS21 encodes a rab5-like GTP binding protein that is required for the sorting of yeast vacuolar proteins. EMBO (Eur. Mol. Biol. Organ.) J. 13:1297-1309.
26. Hori, Y., Y. Takeyama, M. Hiroyoshi, T. Ueda, A. Maeda, H. Ohyanagi, Y. Saitoh, K. Kaibuchi, and Y. Takai. 1996. Possible involvement of Rab11 p24, a ras-like small GTP-binding protein, in intracellular vesicular transport of isolated pancreatic acini. Dig. Dis. Sci. 41:133-138.
27. Jedd, G., C.J. Richardson, R.J. Litt, and N. Segev. 1995. The Ypt1 GTPase is essential for the first two steps of the yeast secretory pathway. J. Cell Biol. 131: 583-590.
28. Jones, S., R.J. Litt, C.J. Richardson, and N. Segev. 1995. Requirement of nucleotide exchange for Ypt1 GTPase mediated protein transport. J. Cell Biol. 130:1051-1061.
29. Jones, S.M., J.R. Crosby, J. Salamero, and K.E. Howell. 1993. A cytosolic complex of p62 and rab6 associates with TGN38/41 and is involved in budding of exocytic vesicles from the trans-Golgi network. J. Cell Biol. 122:775-788.
30. Kaiser, C.A., and R. Schekman. 1990. Distinct sets of SEC genes govern transport vesicle formation and fusion early in the secretory pathway. Cell. 61: 723-733.
31. Kunkel, T.A. 1985. Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc. Natl. Acad. Sci. USA. 82:488-492.
32. Ladinsky, M.S., J.R. Kremer, P.S. Furcinitti, J.R. McIntosh, and K.E. Howell. 1994. HVEM tomography of the trans-Golgi network: structural insights and identification of a lace-like vesicle coat. J. Cell Biol. 127:29-38.
33. Lai, H.M., M. Bard, and R.D. Kirsch. 1994. Identification of a gene encoding a new Ypt/Rab-like monomeric G-protein in Saccharomyces cerevisiae. Yeast. 10:399-402.
34. Leitinger, B., A. Hille-Rehfeld, and M. Spiess. 1995. Biosynthetic transport of the asialoglycoprotein receptor H1 to the cell surface occurs via endosomes. Proc. Natl. Acad. Sci. USA. 92:10109-10113.
35. Lew, D.J., and S.I. Reed. 1993. Morphogenesis in the yeast cell cycle, regulation by Cdc28 and cyclins. J. Cell Biol. 120:1305-1320.
36. Lian, J.P., S. Stone, Y. Jiang, P. Lyons, and S. Ferro-Novick. 1994. Ypt1p implicated in v-SNARE activation. Nature (Lond.). 372:698-701.
37. Lupashin, V.V., S. Hamamoto, and R.W. Schekman. 1996. Biochemical requirements for the targeting and fusion of ER-derived transport vesicles with purified yeast Golgi membranes. J. Cell Biol. 132:277-289.
38. Martinez, O., A. Schmidt, J. Salamero, B. Hoflack, M. Roa, and B. Goud. 1994. The small GTP-binding protein rab6 functions in intra-Golgi transport. J. Cell Biol. 127:1575-1588.
39. Matile, P., H. Moor, and C.F. Robinow. 1969. Yeast cytology. In The Yeasts. A.H. Rose, J.S. Harrison, editors. Academic Press, New York. 1:219-302.
40. Mayer, A., W. Wickner, and A. Haas. 1996. Sec18 (NSF)-driven resease of Sec17p (α-SNAP) can precede docking and fusion of yeast vacuoles. Cell. 85: 83-94.
41. Miyake, S., and M. Yamamoto. 1990. Identification of ras-related, YPT family genes in Schizosaccharomyces pombe. EMBO (Eur. Mol. Biol. Organ.) J. 9:1417-1422.
42. Moya, M., D. Roberts, and P. Novick. 1993. DSS4-1 is a dominant suppressor of sec4-8 that encodes a nucleotide exchange protein that aids Sec4p function. Nature (Lond.). 361:460-463.
43. Mulholland, J., D. Preuss, A. Moon, D. Wong, D. Drubin, and D. Botstein. 1994. Ultrastructure of the yeast cytoskeleton and its association with the plasma membrane. J. Cell Biol. 125:381-391.
44. Narula, N., and J. Stow. 1995. Distinct coated vesicles labeled for p200 bud from trans-Golgi network membranes. Proc. Natl. Acad. Sci. USA. 92:2874-2878.
45. Novick, P., and P. Brennwald. 1993. Friends and family: the role of the Rab GTPases in vesicular traffic. Cell. 75:597-601.
46. Novick, P., C. Field, and R. Schekman. 1980. Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway. Cell. 21:205-215.
47. Novick, P., S. Ferro, and R. Schekman. 1981. Order of events in the yeast secretory pathway. Cell. 25:461-469.
48. Palade, G. 1975. Intracellular aspects of the process of protein synthesis. Science (Wash. DC). 189:347-358.
49. Peter, F., C. Huoffer, S.N. Pind, and W.E. Balch. 1994. Guanine nucleotide dissociation inhibitor is essential for Rab1 function in budding from the endoplasmic reticulum and transport through the Golgi stack. J. Cell Biol. 126: 1393-1406.
50. Pfeffer, S. 1992. GTP-binding proteins in intracellular transport. Trends Cell Biol. 2:41-46.
51. Plutner, H., A.D. Cox, S. Pind, R. Khosravi-Far, J.R. Bourne, R. Schvaninger, C.J. Der, and B. Balch. 1991. Rab1b regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments. J. Cell Biol. 115:31-43.
52. Preuss, D., J. Mulholland, A. Franzusoff, N. Segev, and D. Botstein. 1992. Characterization of the Saccharomyces Golgi complex through the cell cycle by immunoelectron microscopy. Mol. Biol. Cell. 3:789-803.
53. Pringle, J.R., R.A. Preston, A.E.M. Adams, T. Stearns, D. Drubin, B.K. Haarer, and E. Jones. 1989. Fluorescence microscopy methods for yeast. Methods Cell Biol. 31:357-435.
54. Rambourg, A., Y. Clermont, and F. Kepes. 1993. Modulation of the Golgi apparatus in Saccharomyces cerevisiae sec7 mutants as seen by three-dimensional electron microscopy. Anat. Rec. 237:441-452.
55. Redding, K., C. Holcomb, and S.R. Fuller. 1991. Immunolocalization of Kex2 protease identifies a putative late Golgi compartment in the yeast Saccharomyces cerevisiae. J. Cell Biol. 113:527-538.
56. Rexach, M.F., and R.W. Schekman. 1991. Distinct biochemical requirements for the budding, targeting, and fusion of ER-derived transport vesicles. J. Cell Biol. 114:219-229.
57. Rieder, S.E., L.M. Banta, K. Kohrer, M.J. McCaffery, and S.D. Emr. 1996. Multilamellar endosome-like compartment accumulates in the yeast vps28 vacuolar protein sorting mutant. Mol. Biol. Cell. 7:985-999.
58. Rose, M., F. Winston, and P. Heiter. 1990. Methods in Yeast Genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. 198 pp.
59. Rothman, J.E. 1994. Mechanisms of intracellular protein transport. Nature (Lond.). 372:55-63.
60. Rothman, J.E., and F.T. Wieland. 1996. Protein sorting by transport vesicles. Science (Wash. DC). 272:227-234.
61. Schekman, R., and L. Orci. 1996. Coat proteins and vesicle budding. Science (Wash. DC). 271:1526-1538.
62. Segev, N. 1991. Mediation of the attachment or fusion step in vesicular transport by the GTP-binding Ypt1 protein. Science (Wash. DC). 252:1553-1556.
63. Segev, N., and D. Botstein. 1987. The ras-like yeast YPT1 gene is itself essential for growth, sporulation, and starvation response. Mol. Cell. Biol. 7:2367-2377.
64. Segev, N., J. Mulholland, and D. Botstein. 1988. The yeast GTP-binding YPT1 protein and a mammalian counterpart are associated with the secretion machinery. Cell. 52:915-924.
65. Sikorski, R.S., and P. Hieter. 1989. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics. 19:19-27.
66. Søgarrd, M., K. Tani, R.R. Ye, S. Geromanos, P. Tempst, T. Kirchhausen, J.E. Rothman, and T. Söllner. 1994. A rab protein is required for the assembly of SNARE complexes in the docking of transport vesicles. Cell. 78:937-948.
67. Sollner, T. 1995. SNAREs and targeted membrane fusion. FEBS (Fed. Exp. Biol. Organ.) Lett. 369:80-83.
68. Stevens, T., B. Esmon, and R. Schekman. 1982. Early stages in the yeast secretory pathway are required for transport of carboxypeptidase Y to the vacuole. Cell. 30:439-448.
69. Tong, L., A.M. de Vos, M.V. Milburn, J. Jancarik, S. Noguchi, S. Nishimura, K. Miura, E. Ohisuka, and S.-H. Kim. 1989. Structural differences between a ras oncogene protein and the normal protein. Nature (Lond.). 337:90-93.
70. Tooze, S.A., and W.B. Huttner. 1990. Cell-free protein sorting to the regulated and constitutive secretory pathways. Cell. 60:837-847.
71. Tsukada, M., and D. Gallwitz. 1996. Isolation and characterization of SYS genes from yeast, multicopy suppressors of the functional loss of the transport GTPase Ypt6p. J. Cell Sci. 109:2471-2481.
72. Ulrich, O., S. Reinsch, S. Urbe, M. Zerial, and R.G. Parton. 1996. Rab11 regulates recycling through the pericentriolar recycling endosome. J. Cell Biol. 135:913-924.
73. Urbe, S., L.A. Buber, M. Zerial, S.A. Tooze, and R.G. Parton. 1993. Rab11, a small GTPase associated with both constitutive and regulated secretory pathways in PC12 cells. FEBS (Fed. Exp. Biol. Soc.) Lett. 334:175-182.
74. Vida, T.A., and S.D. Emr. 1995. A new vital stain for visualizing vacuolar membrane dynamics and endocytosis in yeast. J. Cell Biol. 128:779-792.
75. Wach, A., A. Brachat, R. Pohlmann, and P. Philippsen. 1994. New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae. Yeast. 10:1793-1808.
76. Walworth, N.C., B. Goud, A.K. Kabcenell, and P.J. Novick. 1989. Mutational analysis of SEC4 suggests a cyclical mechanism for the regulation of vesicular traffic. EMBO (Eur. Mol. Biol. Organ.) J. 8:1685-1693.
77. Wichmann, H., I. Hengst, and D. Gallwitz. 1992. Endocytosis in yeast: evidence for the involvement of a small GTP binding protein (Ypt7). Cell. 71:1131-1142.
78. Zerial, M., and H. Stenmark. 1993. Rab GTPases in vesicular transport. Curr. Opin. Cell Biol. 5:613-620.