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1
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0028946084
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Phylogenetic identification and in situ detection of individual microbial cells without cultivation
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Amann R, Ludwig W, Schleifer KH: Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol Rev 1995, 59:143-169.
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(1995)
Microbiol Rev
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, pp. 143-169
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Amann, R.1
Ludwig, W.2
Schleifer, K.H.3
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2
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0027215468
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The identification of uncultured microbial pathogens
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Relman DA: The identification of uncultured microbial pathogens. J Infect Dis 1993, 168:1-8.
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J Infect Dis
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Relman, D.A.1
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Microbial ecology and evolution: A ribosomal RNA approach
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Annu Rev Microbiol
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Giovannoni, S.J.3
Pace, N.R.4
Stahl, D.A.5
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5
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0030819820
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The RDP (Ribosomal Database Project)
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Maidak BL, Olsen GJ, Larsen N, Overbeek R, McCaughey MJ, Woese CR: The RDP (Ribosomal Database Project). Nucleic Acids Res 1997, 25:109-110. Gives the latest update on RDP. Large updated databases are a prerequisite for rational probe design and consequently of prime importance for the rRNA approach to microbial identification.
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Nucleic Acids Res
, vol.25
, pp. 109-110
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Maidak, B.L.1
Olsen, G.J.2
Larsen, N.3
Overbeek, R.4
McCaughey, M.J.5
Woese, C.R.6
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6
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0030862608
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Database on the structure of small ribosomal subunit RNA
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Van de Peer Y, Jansen J, De Rijk P, De Wachter R: Database on the structure of small ribosomal subunit RNA. Nucleic Acids Res 1997, 25:111-116. The second large database for 16S and 23S rRNA sequences.
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Nucleic Acids Res
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, pp. 111-116
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Van De Peer, Y.1
Jansen, J.2
De Rijk, P.3
De Wachter, R.4
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7
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0025300402
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Towards a natural system of organisms: Proposal for the domains Archaea, Bacteria, and Eucarya
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Woese CR, Kandler O, Wheelis ML: Towards a natural system of organisms: proposal for the domains Archaea, Bacteria, and Eucarya. Proc Natl Acad Sci USA 1990, 87:4576-4579.
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Woese, C.R.1
Kandler, O.2
Wheelis, M.L.3
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8
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0024507744
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Phylogenetic stains: Ribosomal RNA-based probes for the identification of single microbial cells
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DeLong EF, Wickham GS, Pace NR: Phylogenetic stains: ribosomal RNA-based probes for the identification of single microbial cells. Science 1989, 243:1360-1363.
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DeLong, E.F.1
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Pace, N.R.3
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9
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0342711280
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Improved sensitivity of whole cell hybridization by the combination of horseradish peroxidase-labeled oligonucleotides and tyramide signal amplification
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Schönhuber W, Fuchs B, Juretschko S, Amann R: Improved sensitivity of whole cell hybridization by the combination of horseradish peroxidase-labeled oligonucleotides and tyramide signal amplification. Appl Environ Microbiol 1997, 63:3268-3273. Signal amplification using tyramide signal amplification is combined with oligonucleotide probes covalently linked to horseradish peroxidase, making the assay one step shorter,
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(1997)
Appl Environ Microbiol
, vol.63
, pp. 3268-3273
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Schönhuber, W.1
Fuchs, B.2
Juretschko, S.3
Amann, R.4
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10
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0030872095
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A new, sensitive, whole-cell hybridization technique for detection of bacteria involving a biotinylated oligonucleotide probe targeting rRNA and tyramide signal amplification
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Lebaron P, Catala P, Fajon C, Joux F, Baudart J, Bernard L: A new, sensitive, whole-cell hybridization technique for detection of bacteria involving a biotinylated oligonucleotide probe targeting rRNA and tyramide signal amplification. Appl Environ Microbiol 1997, 63:3274-3278, The tyramide signal amplification approach is applied indirectly via biotinylated oligonucleotides, yielding a very sensitive assay.
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Appl Environ Microbiol
, vol.63
, pp. 3274-3278
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Lebaron, P.1
Catala, P.2
Fajon, C.3
Joux, F.4
Baudart, J.5
Bernard, L.6
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11
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0029859085
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An optimized in situ hybridization protocol for planktonic bacteria
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Glöckner FO, Amann R, Alfreider A, Pernthaler J, Psenner R, Trebesius K, Schleifer KH: An optimized in situ hybridization protocol for planktonic bacteria. Syst Appl Microbiol 1996, 19:403-406.
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(1996)
Syst Appl Microbiol
, vol.19
, pp. 403-406
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Glöckner, F.O.1
Amann, R.2
Alfreider, A.3
Pernthaler, J.4
Psenner, R.5
Trebesius, K.6
Schleifer, K.H.7
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12
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0030814448
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Increase in fluorescence intensity of 16S rRNA in situ hybridization in natural samples treated with chloramphenicol
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Ouverney CC, Fuhrman JA: Increase in fluorescence intensity of 16S rRNA in situ hybridization in natural samples treated with chloramphenicol. Appl Environ Microbiol 1997, 63:2735-2740. Boosting the rRNA content ol small environmental cells by a short-term incubation with antibiotics might be a way to increase the fluorescent signal.
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(1997)
Appl Environ Microbiol
, vol.63
, pp. 2735-2740
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Ouverney, C.C.1
Fuhrman, J.A.2
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13
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0031018618
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Identification of bacterial cells by chromosomal painting
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Lanoil BD, Giovannoni SJ: Identification of bacterial cells by chromosomal painting. Appl Environ Microbiol 1997, 63:1118-1123. This is an example of method import in microbiology. Transfer of techniques common to eukaryotic cell biology to microbiology research has proven important and successful.
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(1997)
Appl Environ Microbiol
, vol.63
, pp. 1118-1123
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Lanoil, B.D.1
Giovannoni, S.J.2
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14
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0342813011
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In situ reverse transcription, an approach to characterize genetic diversity and activity of prokaryotes
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Chen F, Gonzalez JM, Dustman WA, Moran MA, Hodson RE: In situ reverse transcription, an approach to characterize genetic diversity and activity of prokaryotes. Appl Environ Microbiol 1997, 63:4907-4913. Another example of the transfer of a technique common to cell biology to microbiology research.
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(1997)
Appl Environ Microbiol
, vol.63
, pp. 4907-4913
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Chen, F.1
Gonzalez, J.M.2
Dustman, W.A.3
Moran, M.A.4
Hodson, R.E.5
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15
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0028862922
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In situ PCR for visualization of microscale distribution of specific genes and gene products in prokaryotic communities
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Hodson RE, Dustman WA, Garg RP, Moran MA: In situ PCR for visualization of microscale distribution of specific genes and gene products in prokaryotic communities. Appl Environ Microbiol 1995, 61:4074-4082.
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Appl Environ Microbiol
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, pp. 4074-4082
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Hodson, R.E.1
Dustman, W.A.2
Garg, R.P.3
Moran, M.A.4
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16
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0031015252
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Obligate intracellular bacterial parasites of acanthamoebae related to Chlamydia spp
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Amann R, Springer N, Schönhuber W, Ludwig W, Schmidt EN, Müller KD, Michel R: Obligate intracellular bacterial parasites of acanthamoebae related to Chlamydia spp. Appl Environ Microbiol 1997, 63:115-121. Continues a series of publications in which bacterial symbionts of protozoa are identified in situ and phylogenetically analyzed in a cultivation-independent approach. This type of study greatly enlarges the knowledge of bacterial diversity.
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(1997)
Appl Environ Microbiol
, vol.63
, pp. 115-121
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Amann, R.1
Springer, N.2
Schönhuber, W.3
Ludwig, W.4
Schmidt, E.N.5
Müller, K.D.6
Michel, R.7
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17
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0031034880
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Molecular identification and localization of filamentous symbiotic bacteria associated with the hydrothermal vent annelid Alvinella pompejana
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Cary SC, Cottrell MT, Stein JL, Camacho F, Desbruyeres D: Molecular identification and localization of filamentous symbiotic bacteria associated with the hydrothermal vent annelid Alvinella pompejana. Appl Environ Microbiol 1997, 63:1124-1130. Beautiful pictures on the red-green discrimination of two closely related bacterial populations.
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(1997)
Appl Environ Microbiol
, vol.63
, pp. 1124-1130
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Cary, S.C.1
Cottrell, M.T.2
Stein, J.L.3
Camacho, F.4
Desbruyeres, D.5
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18
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0030741576
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Phylogenetic analysis and in situ identification of bacteria in activated sludge
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Snaidr J, Amann R, Huber I, Ludwig W, Schleifer KH: Phylogenetic analysis and in situ identification of bacteria in activated sludge. Appl Environ Microbiol 1997, 63:2884-2896. A large study combining 16S rRNA sequence retrieval and fluorescence in situ hybridization with surprising results; for example, the detection of potentially pathogenic members of the genus Arcobacter in high numbers.
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(1997)
Appl Environ Microbiol
, vol.63
, pp. 2884-2896
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Snaidr, J.1
Amann, R.2
Huber, I.3
Ludwig, W.4
Schleifer, K.H.5
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19
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0030763934
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Detection and in situ identification of representatives of a widely distributed new bacterial phylum
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Ludwig W, Bauer SH, Bauer M, Held I, Kirchhof G, Schulze R, Huber I, Spring S, Hartmann A, Schleifer KH: Detection and in situ identification of representatives of a widely distributed new bacterial phylum. FEMS Microbiat Lett 1997, 153:181-190. A whole new bacterial phylum that might be widespread in the environment is revealed by the rRNA approach.
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(1997)
FEMS Microbiat Lett
, vol.153
, pp. 181-190
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Ludwig, W.1
Bauer, S.H.2
Bauer, M.3
Held, I.4
Kirchhof, G.5
Schulze, R.6
Huber, I.7
Spring, S.8
Hartmann, A.9
Schleifer, K.H.10
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20
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0030678621
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Flow cytometric sorting of microorganisms for molecular analysis
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Wallner G, Fuchs B, Beisker W, Spring S, Amann R: Flow cytometric sorting of microorganisms for molecular analysis. Appl Environ Microbiol 1997, 63:4223-4331. Cells can be considerably enriched by flow sorting prior to the retrieval of 16S rRNA. This can make the search for new bacterial diversity more directed.
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(1997)
Appl Environ Microbiol
, vol.63
, pp. 4223-4331
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Wallner, G.1
Fuchs, B.2
Beisker, W.3
Spring, S.4
Amann, R.5
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21
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85030036395
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ARB: a software environment for sequence data on World Wide Web URL: http://www.mikro.biologie.tu-muenchen.de. A freely available, comprehensive software package with, perhaps, currently the best tools for the directed design of rRNA-targeted oligonucleotide probes.
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ARB: A Software Environment for Sequence Data on World Wide Web
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22
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0030839001
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Development and use of fluorescent in situ hybridisation probes for the identification and enumeration of 'Microthrix parvicella' in activated sludge
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Erhart R, Bradford D, Seviour EM, Seviour RJ, Amann RI, Blackall LL: Development and use of fluorescent in situ hybridisation probes for the identification and enumeration of 'Microthrix parvicella' in activated sludge. Syst Appl Microbiol 1997, 20:310-318.
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(1997)
Syst Appl Microbiol
, vol.20
, pp. 310-318
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Erhart, R.1
Bradford, D.2
Seviour, E.M.3
Seviour, R.J.4
Amann, R.I.5
Blackall, L.L.6
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23
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0031030478
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Group-specific small-subunit rRNA hybridization probes to characterize filamentous foaming in activated sludge systems
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De los Reyes FL, Ritter W, Raskin L: Group-specific small-subunit rRNA hybridization probes to characterize filamentous foaming in activated sludge systems. Appl Environ Microbiol 1997, 63:1107-1117.
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(1997)
Appl Environ Microbiol
, vol.63
, pp. 1107-1117
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De Los Reyes, F.L.1
Ritter, W.2
Raskin, L.3
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24
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0030863651
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Group-Specific 16S rRNA-targeted oligonucleotide probes to identify thermophilic bacteria in marine hydrothermal vents
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Harmsen HJM, Prieur D, Jeanthon C: Group-Specific 16S rRNA-targeted oligonucleotide probes to identify thermophilic bacteria in marine hydrothermal vents. Appl Environ Microbiol 1997, 63:4061-4068.
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(1997)
Appl Environ Microbiol
, vol.63
, pp. 4061-4068
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Harmsen, H.J.M.1
Prieur, D.2
Jeanthon, C.3
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25
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0030788862
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Distribution of microorganisms in deep-sea hydrothermal vent chimneys investigated by whole-cell hybridization and enrichment culture of thermophilic subpopulations
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Harmsen HJM, Prieur D, Jeanthon C: Distribution of microorganisms in deep-sea hydrothermal vent chimneys investigated by whole-cell hybridization and enrichment culture of thermophilic subpopulations. Appl Environ Microbiol 1997, 63:2876-2883.
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Appl Environ Microbiol
, vol.63
, pp. 2876-2883
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Harmsen, H.J.M.1
Prieur, D.2
Jeanthon, C.3
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26
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0030970437
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In situ analysis of denitrifying toluene- And m-xylene-degrading bacteria in a diesel fuel-contaminated laboratory aquifer column
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Hess A, Zarda B, Hahn D, Hähner A, Stax D, Höhener P, Zeyer J: In situ analysis of denitrifying toluene- and m-xylene-degrading bacteria in a diesel fuel-contaminated laboratory aquifer column. Appl Environ Microbiol 1997, 63:2136-2141. One example of a type of study that should be done more often, addressing the key question: 'Are my lab isolates relevant in nature?'
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(1997)
Appl Environ Microbiol
, vol.63
, pp. 2136-2141
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Hess, A.1
Zarda, B.2
Hahn, D.3
Hähner, A.4
Stax, D.5
Höhener, P.6
Zeyer, J.7
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27
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0030716777
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Isolation of new bacterial species from drinking water biofiims and proof of their in situ dominance with highly specific 16S rRNA probes
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Kalmbach S, Manz W, Szewzyk U: Isolation of new bacterial species from drinking water biofiims and proof of their in situ dominance with highly specific 16S rRNA probes. Appl Environ Microbiol 1997, 63:4164-4170. The combination of traditional cultivation and fluorescence in situ hybridization used in this study results in a very powerful approach to describe bacterial diversity and community structure.
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(1997)
Appl Environ Microbiol
, vol.63
, pp. 4164-4170
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Kalmbach, S.1
Manz, W.2
Szewzyk, U.3
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0030890090
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Use of green fluorescent protein as a marker for ecological studies of activated sludge communities
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Eberl L, Schulze R, Ammendola A, Geisenberger O, Erhart R, Sternberg C, Molin S, Amann R: Use of green fluorescent protein as a marker for ecological studies of activated sludge communities. FEMS Microbiol Lett 1997, 149:77-83. Combines green fluorescent protein based in vivo monitoring with fluores-cence in situ hybridization in a nice methological study.
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(1997)
FEMS Microbiol Lett
, vol.149
, pp. 77-83
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Eberl, L.1
Schulze, R.2
Ammendola, A.3
Geisenberger, O.4
Erhart, R.5
Sternberg, C.6
Molin, S.7
Amann, R.8
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What size should a bacterium be? A question of scale
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Die Mikrowelt der Meeresbakterien
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Jergensen BB: Die Mikrowelt der Meeresbakterien. Naturwissenschaften 1995, 82:269-278. [Title translation: The microworld of marine bacteria.]
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Jergensen BB: Life in the diffusive boundary layer. In The Benthic Boundary Layer. Edited by Boudreau BP, Jørgensen BB. Oxford: Oxford University Press; 1998: in press. An excellent introduction and review of the physicochemical properties of the microbial world and the adaptation of microorganisms to these conditions.
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The Benthic Boundary Layer
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Optical properties of microbial mats: Light measurements with fiber-optic microprobes
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Microbial Mats: Structure, Development, and Environmental Significance
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Kühl, M.1
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Microsensors for the study of interfacial biogeochemical processes
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Edited by Boudreau BP, Jørgensen BB. Oxford: Oxford University Press; in press
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Kühl M, Revsbech NP: Microsensors for the study of interfacial biogeochemical processes. In The Benthic Boundary Layer. Edited by Boudreau BP, Jørgensen BB. Oxford: Oxford University Press; 1998: in press. The most recent review of microsensor techniques available for environmental analysis.
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The Benthic Boundary Layer
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Optical measurement of oxygen and temperature in microscale: Strategies and biological applications
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Klimant, I.1
Kühl, M.2
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A nitrite microsensor for profiling environmental biofilms
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De Beer D, Schramm A, Santegoeds CM, Kühl M: A nitrite microsensor for profiling environmental biofilms. Appl Environ Microbiol 1997, 63:973-977. A very detailed data set on oxygen consumption, nitrogen and sulfur cycling in a biofilm, where first data on the role of nitrite in these processes are presentad.
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Appl Environ Microbiol
, vol.63
, pp. 973-977
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De Beer, D.1
Schramm, A.2
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Kühl, M.4
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40
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0031427567
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2 microelectrode for profiling in sediments, microbial mats and biofilms
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2 microelectrode for profiling in sediments, microbial mats and biofilms. Limnol Oceanogr 1997, 42:1590-1600.
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Limnol Oceanogr
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De Beer, D.1
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Kühl, M.4
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0031186673
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A microscale biosensor for methane containing methanotrophic bacteria and an internal oxygen reservoir
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Damgaard LR, Revsbech NP: A microscale biosensor for methane containing methanotrophic bacteria and an internal oxygen reservoir. Anal Chem 1997, 69:2262-2267. The first microprofiles of methane in biofilms and sediments were measured with a new microbiosensor, which works with methane oxidizers kept in a gradient microchamber at the sensor tip. Clever use of microbiology in designing of a new class of biosensors.
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Anal Chem
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, pp. 2262-2267
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Damgaard, L.R.1
Revsbech, N.P.2
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0031236843
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- biosensor for environmental applications
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- biosensor for environmental applications. Anal Chem 1997, 69:3527-3531. A new biosensor based on a special mutation of a denitrifying baderium. The sensor tip contains a microscale chemostat, whereby a nitrate microsensor for use in both marine and freshwater environments was realised.
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Anal Chem
, vol.69
, pp. 3527-3531
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Larsen, L.H.1
Kjær, T.2
Revsbech, N.P.3
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0030827156
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Hydrogen concentration profiles at the oxic-anoxic interface: A microsensor study of the hindgut of the wood-feeding lower termite Reticulitermes flavipes (Kollar)
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Ebert A, Brune A: Hydrogen concentration profiles at the oxic-anoxic interface: A microsensor study of the hindgut of the wood-feeding lower termite Reticulitermes flavipes (Kollar). Appl Environ Microbiol 1997, 63:4039-4046. Microsensor measurements demonstrate the presence of steep opposing gradients of oxygen and hydrogen in the termite gut. This paper gives a totally new picture of the termite gut microenvironment along with some microbiological considerations about the importance of various metabolic processes.
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(1997)
Appl Environ Microbiol
, vol.63
, pp. 4039-4046
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Ebert, A.1
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46
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Oxygen dynamics at the base of a biofilm studied with planar optrodes
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Glud RN, Santegoeds CM, De Beer D, Kohls O, Ramsing NB: Oxygen dynamics at the base of a biofilm studied with planar optrodes. Aq Microb Ecol 1998, in press. In this study a new technique for imaging the two-dimensional oxygen distribution at high spatial resolution is outlined and demonstrated for the first time in heterogeneous biofilms. It represents an elegant approach for measuring distribution in various heterogeneous natural samples and consequently bears high potential for many applications in microbiology.
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(1998)
Aq Microb Ecol
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Glud, R.N.1
Santegoeds, C.M.2
De Beer, D.3
Kohls, O.4
Ramsing, N.B.5
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0026637716
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Effect of acetylene on nitrous oxide reduction and sulfide oxidation in batch and gradient cultures of Thiobacillus denitrificans
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Growth pattern and yield of a chemoautotrophic Beggiatoa sp. in oxygen-sulfide microgradients
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A benthic gradient chamber for culturing phototrophic sulfur bacteria on reconstitued sediments
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2642671981
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Growth of green sulfur bacteria in experimental benthic oxygen, sulfide, pH and light gradients
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in press
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Pringault O, Kühl M, de Wit R, Caumette P: Growth of green sulfur bacteria in experimental benthic oxygen, sulfide, pH and light gradients. Microbiology 1998, in press.
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(1998)
Microbiology
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Pringault, O.1
Kühl, M.2
De Wit, R.3
Caumette, P.4
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52
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0030741940
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The response of microbial populations from oil-brine contaminated soil to gradients of NaCl and sodium p-toluate in a diffusion gradient chamber
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Emerson D, Breznak JA: The response of microbial populations from oil-brine contaminated soil to gradients of NaCl and sodium p-toluate in a diffusion gradient chamber. FEMS Microbiol Ecol 1997, 23:285-300. A new gradient growth system is used in combination with microsensors and microbiological and molecular techniques. One of very few examples in which all these techniques are used in concert.
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(1997)
FEMS Microbiol Ecol
, vol.23
, pp. 285-300
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Emerson, D.1
Breznak, J.A.2
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53
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0030995331
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A sulfate-reducing bacterium from the oxic layer of a microbial mat from Solar Lake (Sinai), Desulfovibrio oxydinae sp. nov
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Krekeler D, Sigalevich P, Teske A, Cypionka H, Cohen Y: A sulfate-reducing bacterium from the oxic layer of a microbial mat from Solar Lake (Sinai), Desulfovibrio oxydinae sp. nov. Arch Microbiol 1997, 167:369-375.
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(1997)
Arch Microbiol
, vol.167
, pp. 369-375
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Krekeler, D.1
Sigalevich, P.2
Teske, A.3
Cypionka, H.4
Cohen, Y.5
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54
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0030961164
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Oxygen-dependent growth of the obligate anaerobe Desulfovibrio vulgaris Hildenborough
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Johnson MS, Zhulin IB, Gapuzan M-ER, Taylor BL: Oxygen-dependent growth of the obligate anaerobe Desulfovibrio vulgaris Hildenborough. J Bacteriol 1997, 179:5598-5601.
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(1997)
J Bacteriol
, vol.179
, pp. 5598-5601
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Johnson, M.S.1
Zhulin, I.B.2
Gapuzan, M.-E.R.3
Taylor, B.L.4
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55
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0000414321
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Anoxic aggregates an ephemeral phenomenon in the pelagic environment?
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Ploug H, Kühl M, Buchholz-Cleven B, Jørgensen BB: Anoxic aggregates an ephemeral phenomenon in the pelagic environment? Aq Microb Ecol 1997, 13:285-294. Combined microsensor and molecular analysis of marine aggregates. Detailed measurements and analysis of oxygen microgradients indicate why sulfate-reducing bacteria and methanogens do not thrive in anoxic niches of aggregates.
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(1997)
Aq Microb Ecol
, vol.13
, pp. 285-294
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Ploug, H.1
Kühl, M.2
Buchholz-Cleven, B.3
Jørgensen, B.B.4
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56
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0030779914
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Application of microautoradiography to the study of substrate uptake by filamentous microorganisms in activated sludge
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Andreasen K, Nielsen PH: Application of microautoradiography to the study of substrate uptake by filamentous microorganisms in activated sludge. Appl Environ Microbiol 1997, 63:3662-3666. The old technique of microautoradiography is still indispensable if performed with patience and skill.
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(1997)
Appl Environ Microbiol
, vol.63
, pp. 3662-3666
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Andreasen, K.1
Nielsen, P.H.2
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57
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0030620894
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Determination of active marine bacterioplankton: A comparison of universal 16S rRNA probes, autoradiography, and nucleoid staining
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Karner M, Fuhrman JA: Determination of active marine bacterioplankton: a comparison of universal 16S rRNA probes, autoradiography, and nucleoid staining. Appl Environ Microbiol 1997, 63:1208-1213. An attempt to show that most that we see out there in aquatic samples with a microscope, in terms of bacteria, is still alive and active.
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(1997)
Appl Environ Microbiol
, vol.63
, pp. 1208-1213
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Karner, M.1
Fuhrman, J.A.2
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58
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0030688049
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Visualization of specific gene expression in individual Salmonella typhimurium cells by in situ PCR
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Tolker-Nielsen T, Holmstrom K, Molin S: Visualization of specific gene expression in individual Salmonella typhimurium cells by in situ PCR, Appl Environ Microbiol 1997, 63:4196-4203. Confirms the potential of in situ PCR for the visualization of specific gene expression in bacteria.
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(1997)
Appl Environ Microbiol
, vol.63
, pp. 4196-4203
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Tolker-Nielsen, T.1
Holmstrom, K.2
Molin, S.3
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59
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0030923197
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An immunological approach to detect phosphate stress in populations and single cells of photo synthetic picoplankton
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Scanlan DJ, Silman NJ, Donald KM, Wilson WH, Carr NG, Joint I, Mann NH: An immunological approach to detect phosphate stress in populations and single cells of photo synthetic picoplankton. Appl Environ Microbiol 1997, 63:2411-2420. Antibodies are the most direct way to monitor proteins that catalyze defined cellular activities in situ. Here, immunofluorescence was used to show that individual cells have experienced phosphate stress.
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(1997)
Appl Environ Microbiol
, vol.63
, pp. 2411-2420
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Scanlan, D.J.1
Silman, N.J.2
Donald, K.M.3
Wilson, W.H.4
Carr, N.G.5
Joint, I.6
Mann, N.H.7
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60
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0030849481
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Development and characterization of a whole-cell bioluminescent sensor for bioavailable middle-chain alkanes in contaminated groundwater samples
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Sticher P, Jaspera MCM, Stemmler K, Harms H, Zehnder AJB, van der Meer JR: Development and characterization of a whole-cell bioluminescent sensor for bioavailable middle-chain alkanes in contaminated groundwater samples. Appl Environ Microbiol 1997, 63:4053-4060.
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(1997)
Appl Environ Microbiol
, vol.63
, pp. 4053-4060
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Sticher, P.1
Jaspera, M.C.M.2
Stemmler, K.3
Harms, H.4
Zehnder, A.J.B.5
Van Der Meer, J.R.6
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61
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0030779915
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Simultaneous determination of gene expression and bacterial identity in single cells in defined mixtures of pure cultures
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Poulsen LK, Dalton HM, Angles ML, Marshall KC, Molin S, Goodman AE: Simultaneous determination of gene expression and bacterial identity in single cells in defined mixtures of pure cultures. Appl Environ Microbiol 1997, 63:3698-3702. A methodological paper showing how a combination of in situ identification and activity monitoring could, in the future, work in environmental samples.
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(1997)
Appl Environ Microbiol
, vol.63
, pp. 3698-3702
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Poulsen, L.K.1
Dalton, H.M.2
Angles, M.L.3
Marshall, K.C.4
Molin, S.5
Goodman, A.E.6
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62
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0027375701
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2S in photosynthetic biofilms determined by oligonucleotide probes and microelectrodes
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2S in photosynthetic biofilms determined by oligonucleotide probes and microelectrodes. Appl Environ Microbiol 1993, 59:3820-3849.
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(1993)
Appl Environ Microbiol
, vol.59
, pp. 3820-3849
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Ramsing, N.B.1
Kühl, M.2
Jørgensen, B.B.3
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63
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0029659250
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Structure and function of a nitrifying biofilm as determined by in situ hybridization and microelectrodes
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Schramm A, Larsen LH, Revsbech NP, Ramsing NB, Amann R, Schleifer KH: Structure and function of a nitrifying biofilm as determined by in situ hybridization and microelectrodes. Appl Environ Microbiol 1996, 62:4641-4647.
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(1996)
Appl Environ Microbiol
, vol.62
, pp. 4641-4647
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Schramm, A.1
Larsen, L.H.2
Revsbech, N.P.3
Ramsing, N.B.4
Amann, R.5
Schleifer, K.H.6
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64
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0031465029
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Biodiversity within hot spring microbial mat communities; molecular monitoring of enrichment cultures
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Ward DM, Santegoeds CM, Nold SC, Ramsing NB, Ferris MJ, Bateson MM: Biodiversity within hot spring microbial mat communities; molecular monitoring of enrichment cultures. Antonie van Leeuwenhoek 1997, 71:143-150. Excellent review of how the use of molecular techniques can reveal more details on the population structure of a natural microbial community than the information obtained from traditional microbiological techniques, and how enrichment procedures can bias the obtained view of the community structure.
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(1997)
Antonie Van Leeuwenhoek
, vol.71
, pp. 143-150
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Ward, D.M.1
Santegoeds, C.M.2
Nold, S.C.3
Ramsing, N.B.4
Ferris, M.J.5
Bateson, M.M.6
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65
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0030613348
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Seasonal distributions of dominant 16S rRNA-defined populations in a hot spring microbial mat examined by denaturing gradient gel electrophoresis
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Ferris MJ, Ward DM: Seasonal distributions of dominant 16S rRNA-defined populations in a hot spring microbial mat examined by denaturing gradient gel electrophoresis. Appl Environ Microbiol 1997, 63:1375-1381. A detailed field study of cyanobacterial populations and their distribution in a hot spring environment. An example of molecular ecology in the field.
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(1997)
Appl Environ Microbiol
, vol.63
, pp. 1375-1381
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Ferris, M.J.1
Ward, D.M.2
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66
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0030613231
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Population structure and physiological changes within a hot spring microbial mat community following disturbance
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Ferris MJ, Mold SC, Revsbech NP, Ward DM: Population structure and physiological changes within a hot spring microbial mat community following disturbance. Appl Environ Microbiol 1997, 63:1367-1374. Combined use of molecular techniques and microsensors to monitor changes in population structure and activity of phototrophic microbial community during a disturbance experiment. A good example of what can be done by such a combined approach in microbial ecology.
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(1997)
Appl Environ Microbiol
, vol.63
, pp. 1367-1374
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Ferris, M.J.1
Mold, S.C.2
Revsbech, N.P.3
Ward, D.M.4
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