Visualization of specific gene expression in individual Salmonella typhimurium cells by in situ PCR

Applied and Environmental Microbiology

Volumn 63, Issue 11, 1997, Pages 4196-4203

  Tolker Nielsen, Tim ^a   Holmstrøm, Kim ^b   Molin, Søren ^a  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^b Biotechnological Institute Denmark   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

ANALYTIC METHOD; ANTIBIOTIC RESISTANCE; ARTICLE; CELL MEMBRANE PERMEABILITY; ENZYME SUBSTRATE; GENE EXPRESSION REGULATION; METABOLIC ACTIVATION; NONHUMAN; POLYMERASE CHAIN REACTION; REPORTER GENE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SALMONELLA TYPHIMURIUM;

EID: 0030688049     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.63.11.4196-4203.1997     Document Type: Article

References (46)

1. Amann, R. I., L. Krumholz, and D. A. Stahl. 1990. Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology. J. Bacteriol. 172:762-770.
2. Amann, R. I., J. Stromley, R. Devereux, R. Key, and D. A. Stahl. 1992. Molecular and microscopic identification of sulfate-reducing bacteria in multispecies biofilms. Appl. Environ. Microbiol. 58:614-623.
3. Bagasra, O. 1990. Polymerase chain reaction in situ. Amplification March: 20-21.
4. Bagasra, O., S. P. Hauptman, H. W. Lischer, M. Sachs, and R. J. Pomerantz. 1992. Detection of human immunodeficiency virus type 1 provirus in mononuclear cells by in situ polymerase chain reaction. N. Engl. J. Med. 326:1385-1391.
5. Bagasra, O., T. Seshamma, J. Hansen, L. Bobroski, P. Saikumari, and R. J. Pomerantz. 1995. Applications of in situ PCR methods in molecular biology, p. 35-68. In J. Gu (ed.). In situ polymerase chain reaction and related technology. Eaton Publishing Co., Natick, Mass.
6. Barer, M. R. 1991. New possibilities for bacterial cytochemistry: light microscopical demonstration of beta-galactosidase in unfixed immobilized bacteria. Histochem. J. 23:529-533.
7. Belas, R., A. Mileham, D. Cohn, M. Hilmen, M. Simon, and M. Silverman. 1982. Bacterial bioluminescence: isolation and expression of the luciferase genes from Vibrio harveyi. Science 218:791-793.
8. Bertani, G. 1951. Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli. J. Bacteriol. 62:293-300.
9. Chalfie, M., Y. Tu, G. Euskirchen, W. W. Ward, and D. C. Prasher. 1994. Green fluorescent protein as a marker for gene expression. Science 263:802-805.
10. Clark, D. J., and O. Maaløe. 1967. DNA replication and the division cycle in Escherichia coli. J. Mol. Biol. 23:99-112.
11. DeLong, E. F., G. S. Wickham, and N. R. Pace. 1989. Phylogenetic stains: ribosomal RNA-based probes for the identification of single cells. Science 243:1360-1363.
12. Deuschle, U., W. Kammerer, R. Gentz, and H. Bujard. 1986. Promoters of Escherichia coli: a hierarchy of in vivo strength indicates alternate structures. EMBO J. 5:2987-2994.
13. Diaper, J.-P., K. Tither, and C. Edwards. 1992. Rapid assessment of bacterial viability by flow cytometry. Appl. Microbiol. Biotechnol. 38:268-272.
14. Enomoto, M., and B. A. D. Stocker. 1974. Transduction by phage P1kc in Salmonella typhimurium. Virology 60:503-514.
15. Haase, A. T., E. F. Retzel, and K. A. Starkus. 1990. Amplification and detection of lentiviral DNA inside cells. Proc. Natl. Acad. Sci. USA 87:4971-4975.
16. Hahn, D., R. I. Amann, and J. Zeyer. 1993. Detection of mRNA in Streptomyces cells by whole-cell hybridization with digoxigenin-labeled probes. Appl. Environ. Microbiol. 59:2753-2757.
17. Hamkalo, B. A., and O. L. Miller, Jr. 1973. Electron microscopy of genetic activity. Annu. Rev. Biochem. 42:379-396.
18. Hodson, R. E., W. A. Dustman, R. P. Garg, and M. A. Moran. 1995. In situ PCR for visualization of microscale distribution of specific genes and gene products in prokaryotic communities. Appl. Environ. Microbiol. 61:4074-4082.
19. Hönerlage, W., D. Hahn, and J. Zeyer. 1995. Detection of mRNA of nprM in Bacillus megaterium ATCC 14581 grown in soil by whole-cell hybridization. Arch. Microbiol. 163:235-241.
20. Iturriaga, R. 1979. Bacterial activity related to sedimenting particulate matter. Mar. Biol. 55:157-169.
21. Kaprelyants, A. S., and D. B. Kell. 1992. Rapid assessment of bacterial viability and vitality by rhodamine 123 and flow cytometry. J. Appl. Bacteriol. 72:410-422.
22. Kaprelyants, A. S., and D. B. Kell. 1993. The use of 5-cyano-2,3-ditolyl tetrazolium chloride and flow cytometry for the visualization of respiratory activity in individual cells of Micrococcus luteus. J. Microbiol. Methods 17: 115-122.
23. Kremer, L., A. Baulard, J. Estaquier, O. Poulain-Godefroy, and C. Locht. 1995. Green fluorescent protein as a new expression marker in mycobacteria. Mol. Microbiol. 17:913-922.
24. Lewis, P. J., C. E. Nwoguh, M. R. Barer, C. R. Harwood, and J. Errington. 1994. Use of digitized video microscopy with a fluorogenic enzyme substrate to demonstrate cell- and compartment-specific gene expression in Salmonella enteritidis and Bacillus subtilis. Mol. Microbiol. 13:655-662.
25. Maloney, P. C., and B. Rotman. 1973. Distribution of suboptimally induced β-D-galactosidase in Escherichia coli. The enzyme content of individual cells. J. Mol. Biol. 73:77-91.
26. Marguet, E., and P. Forterre. 1994. DNA stability at temperatures typical for hyperthermophiles. Nucleic Acids Res. 22:1681-1686.
27. Matsuyama, T. 1984. Staining of living bacteria with rhodamine 123. FEMS Microbiol. Lett. 77:990-994.
28. Miao F., P. Todd, and D. S. Kompala. 1993. A single-cell assay of β-galactosidase in recombinant Escherichia coli using flow cytometry. Biotechnol. Bioeng. 42:708-715.
29. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
30. Møller, S., C. S. Kristensen, L. K. Poulsen, J. M. Carstensen, and S. Molin. 1995. Bacterial growth on surfaces: automated image analysis for quantification of growth rate-related parameters. Appl. Environ. Microbiol. 61:741-748.
31. Møller, S., A. R. Pedersen, L. K. Poulsen, E. Arvin, and S. Molin. 1996. Activity and three-dimensional distribution of toluene-degrading Pseudomonas putida in a multispecies biofilm assessed by quantitative in situ hybridization and scanning confocal laser microscopy. Appl. Environ. Microbiol. 62:4632-4640.
32. Myers, T. W., and C. L. Sigua. 1995. Amplification of RNA: high temperature reverse transcription and DNA amplification with Thermus thermophilus DNA polymerase, p. 58-68. In M. A. Innis, D. H. Gelfand, and J. J. Sninsky (ed.), PCR strategies. Academic Press, San Diego, Calif.
33. Novick, A., and M. Weiner. 1957. Enzyme induction as an all-or-none phenomenon. Proc. Natl. Acad. Sci. USA 43:553-566.
34. Nuovo, G. J., P. MacConnell, A. Forde, and P. Delvenne. 1991. Detection of human papillomavirus DNA in formalin fixed tissues by in situ hybridization after amplification by PCR. Am. J. Pathol. 139:847-854.
35. Nuovo, G. J., F. Gallery, P. MacConnell, P. Becker, and W. Bloch. 1991. An improved technique for the detection of DNA by in situ hybridization after PCR amplification. Am. J. Pathol. 139:1239-1244.
36. Patel, B. K. R., D. K. Banerjee, and P. D. Butcher. 1993. Determination of Mycobacterium leprae viability by polymerase chain reaction amplification of 71-kDa heat-shock protein mRNA. J. Infect. Dis. 168:799-800.
37. Petersen, C. 1991. Multiple determinants of functional mRNA stability: sequence alterations at either end of the lacZ gene affect the rate of mRNA inactivation. J. Bacteriol. 173:2167-2172.
38. Poulsen, L. K., G. Ballard, and D. A. Stahl. 1993. Use of rRNA fluorescence in situ hybridization for measuring the activity of single cells in young and established biofilms. Appl. Environ. Microbiol. 59:1354-1360.
39. Poulsen, L. K., H. M. Dalton, M. L. Angles, K. C. Marshall, S. Molin, and A. E. Goodman. Simultaneous determination of gene expression and bacterial identity in single cells. Submitted for publication.
40. Rodriguez, G. G., D. Phipps, K. Ishiguro, and H. F. Ridgway. 1992. Use of fluorescent redox probe for direct visualization of actively respiring bacteria. Appl. Environ. Microbiol. 58:1801-1808.
41. Shapiro, J. A. 1977. F, the E. coli sex factor, p. 613. In A. I. Bukhari, J. A. Shapiro, and S. L. Adhya (ed.), DNA, insertion elements, plasmids and episomes. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
42. Stahl, D. A., and R. I. Amann. 1991. Development and application of nucleic acid probes, p. 205-248. In E. Stackebrandt and M. Goodfellow (ed.), Nucleic acid techniques in bacterial systematics. John Wiley, New York, N.Y.
43. Stewart, G. S., and P. Williams. 1992. lux genes and the applications of bacterial bioluminescence. J. Gen. Microbiol. 138:1289-1300.
44. Tolker-Nielsen, T., K. Holmstrøm, and S. Molin. Non-genetic population heterogeneity studied by in situ PCR. Submitted for publication.
45. Wallner, G., R. Erhart, and R. Amann. 1995. Flow cytometric analysis of activated sludge with rRNA-targeted probes. Appl. Environ. Microbiol. 61: 1859-1866.
46. Webb, C. D., A. Decatur, A. Teleman, and R. Losick. 1995. Use of green fluorescent protein for visualization of cell-specific gene expression and subcellular protein localization during sporulation in Bacillus subtilis. J. Bacteriol. 177:5906-5911.