Enhanced proliferation of human fibroblasts, in the presence of dexamethasone, is accompanied by changes in p21(Waf1/Cip1/Sdi1) and the insulin-like growth factor type 1 receptor

Journal of Cellular Physiology

Volumn 177, Issue 3, 1998, Pages 396-401

  Li, Shu ^a   Mawal Dewan, Madhumalti ^a   Cristofalo, Vincent J ^a   Sell, Christian ^a  

^a DREXEL UNIVERSITY COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DEXAMETHASONE; PROTEIN P21; SOMATOMEDIN C RECEPTOR;

ARTICLE; CELL CYCLE; CELL PROLIFERATION; CONTROLLED STUDY; FIBROBLAST CULTURE; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DETERMINATION;

CELL DIVISION; CELL LINE; CYCLIN-DEPENDENT KINASE INHIBITOR P21; CYCLINS; DEXAMETHASONE; FIBROBLASTS; GLUCOCORTICOIDS; HUMANS; INSULIN-LIKE GROWTH FACTOR I; RECEPTORS, SOMATOMEDIN; RNA, MESSENGER;

EID: 0031727728     PISSN: 00219541     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-4652(199812)177:3<396::AID-JCP3>3.0.CO;2-K     Document Type: Article

References (35)

1. Afshari, C.A., Vojta, P.J., Annab, L.A., Futreal, P.A., Willard, T.B., and Barrett, J.C. (1993) Investigation of the role of'G1/S cell cycle mediators in cellular senescence. Exp. Cell. Res., 209:231-237.
2. Alcorta, D.A., Xiong, Y., Phelps, D., Hannon, G., Beach, D., and Barret, J.C. (1996) Involvement of the cyclin dependent kinase inhibitor p16(INK4a) in replicative senescence of norma human fibroblasts. Proc. Natl. Acad. Sci. USA, 93:13742-13747.
3. Atadia, P., Wong, H., Garkavtsev, I., Villette, C., and Riabowol, K. (1995) Increased activity of p53 in senescing fibroblasts. Proc. Natl. Acad. Sci. USA, 92:8348-8352.
4. Billard, C., and Nardeux, P. (1983) Analysis of ribosomal RNA in human fibroblasts continuously treated with hydrocortisone. Exp. Cell Res., 147:431-435
5. Bond, J.A., Blaydes, J.P., Rowson, J., Haughton, M.F., Smith, J.R., Wynford-Thomas, D., and Wyllie, F.S. (1995) Mutant p53 rescues human diploid cells from serescence without inhibiting the induction of SDI/WAF1. Cancer Res., 55:2204-2209.
6. Brown, J.P., Wei, W., and Sedivy, J.M. (1997) Bypass of senescence after disruption of p21CIP1/WAF1 gene in normal diploid human fibroblasts. Science, 277:831-834.
7. Campisi, J., Dimri, G., and Hara, E. (1996) Control of replicative senescence. In: Handbook of the Biology of Aging. Schneider and Rowen, eds. Academic Press, San Diego, pp. 121-149.
8. Chomczynski, P., and Sacchi, N. (1987) A single step method for the isolation of total RNA from mammalian cells. Anal. Biochem., 162:156-159.
9. Clemmons, D.R., Busby, W.H., Arai, T., Nam, T.J., Clarke, J.B., Jones, J.I., and Ankrapp, D.K. (1995) Role of insulin like growth factor binding proteins in the control of IGF actions. Prog. Growth Factor Res., 6:357-366.
10. Conover, C.A. (1996) Regulation and physiologic control of insulin like growth factor binding proteins. Endocr. J., 43:43-48.
11. Conover, C.A., Dollar, L.A., Hintz, R.L., and Rosenfeld, R.G. (1983) Insulin like growth factor/somatomedin C and glucocorticoids synergistically regulate mitosis in competent human fibroblasts. J. Cell. Physiol., 116:191-197.
12. Conover, C.A., Rosenfeld, R.G., and Hintz, R.L. (1989) Serum glucocorticoids have a persistant and controlling effect on insulin like growth factor 1 action under serum free assay conditions in cultured human fibroblasts. In Vitro Cell. Dev. Biol., 25:521-527.
13. Cristofalo, V.J. (1975) The effect of hydrocortisone on DNA synthesis and cell division during aging in vitro. In: Cell Impairment in Aging and Development. V.J. Cristofalo and E. Holeckova, eds. Plenum Press, New York, pp. 7-22.
14. Cristofalo, V.J., Wallace, J.M., and Rosner, B.A. (1979) Glucocorticoid enhancement of proliferative activity in WI-38 cells. In: Hormones and Cell Culture. Cold Spring Harbor Conferences on Cell Proliferation. Cold Spring Harbor Press, Cold Spring Harbor NY Vol. 6, pp. 875-887.
15. Cristofalo, V.J., and Charpentier, R.J. (1980) A standard method for cultivating human diploid fibroblast like cells to study cellular aging. J. Tissue Cult. Methods, 6:117.
16. Cristofalo, V.J., and Pignolo, R. (1993) Replicative senescence of human fibroblast like cells in culture. Physiol. Rev., 73:617-638.
17. Cristofalo, V.J., and Pignolo, R. (1996) Molecular markers of senescence in fibroblast like cultures. Exp. Gerontol., 31:111-123.
18. nd Edition, pp. 77-113.
19. El-Diery, W.S., Tokino, T., Velculescu, V.E., Levy, D.B., Parsons, R., Trent, J., Lin, D., Mercer, W.E., Kinzler, K.W., and Vogelstein, B. (1993) WAF1, a potential mediator of p53 tumor suppression. Cell, 75:817-825.
20. Finlay, C.A., and Cristofalo, V.J. (1987) Autocrine stimulation of WI-38 cell proliferation in the presence of glucocorticoids. Exp. Cell Res., 168:191-202.
21. Finlay, C.A., Rosner, B.A., Miller, J.E., Wallace, J.M., and Cristofalo, V.J. (1985) Enhanced proliferation of WI-38 cells in the presence of glucocorticoid conditioned medium. Exp. Cell Res., 156:462-470.
22. Giorgino, F., and Smith, R.J. (1995) Dexamethasone enhances insulin like growth factor effects on skeletal muscle cell proliferation. Clin Invest., 93:1473-1483.
23. CDKN2 expression and its implications for cell immortalization and senescence. Mol. Cell. Biol., 16:859-867.
24. Hayflick, L., and Moorhead, P.S. (1961) The serial cultivation of human diploid fibroblast strains. Exp. Cell Res., 25:585-621.
25. Kaleko, M., Rutter, W.J., and Miller, A.D. (1990) Overexpression of the human insulin like growth factor I receptor promotes ligand-dependent neoplastic transformation. Mol. Cell. Biol., 10:464-473.
26. Macieira-Coehlo, A. (1966) Action of cortisone on human fibroblasts in vitro. Experientia, 22:390-391.
27. Medcalf, A.S., Klein-Szanto, A.J., and Cristofalo, V.J. (1996) Expression of p21 is not required for senescence of human fibroblasts. Cancer Res., 50:4582-4585.
28. SDI antisense RNA. Proc. Natl. Acad. Sci. USA, 92:4352-4356.
29. Noda, A., Ning, Y., Venable, S.F., Periera-Smith, O.M., and Smith, J.R. (1994) Cloning of senescent derived inhibitors of DNA synthesis using an expression screen. Exp. Cell Res., 211:90-98.
30. Phillips, P.D., and Cristofalo, V.J. (1988) Classification system based on the functional equivalency of mitogens that regulate WI-38 proliferation. Exp. Cell Res., 175:396-403.
31. Reznikoff, C.A., Yeager, T.R., Belair, C.D., Savelieva, E., Puthenveettil, J.A., and Stadler, W.M. (1996) Elevated p16 at senescence and loss of p16 at immortalization in human papillomavirus 16 E6 but not E7 transformed uroepithelial cells. Cancer Res., 56:2886-2890.
32. Rosner, B.A., and Cristofalo, V.J. (1979) Hydrocortisone: A specific modulator of in vitro cell proliferation and aging. Mech. Ageing Dev., 9:485-496.
33. Rosner, B.A., and Cristofalo, V.J. (1981) Changes in dexamethasone specific binding during aging in WI-38 cells. Endocinology, 108:1965-1971.
34. Werner, H., Bach, M.A., Stannard, B., Roberts, C.T., Jr., LeRoith, D. (1992) Structural and functional analysis of the insulin like growth factor 1 receptor promoter. Mol. Endocinol., 6:1545-1558.
35. Waf1/Cip1/Sdi1 halts hepatocyte cell cycle progression, post-natal liver development, and regeneration. Genes Dev., 10:245-260.