Expression of p21 is not required for senescence of human fibroblasts

Cancer Research

Volumn 56, Issue 20, 1996, Pages 4582-4585

  Medcalf, Andrew S C ^a   Klein Szanto, Andres J P ^b   Cristofalo, Vincent J ^a  

^a DREXEL UNIVERSITY COLLEGE OF MEDICINE   (United States)

^b FOX CHASE CANCER CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEIN P21;

ARTICLE; CELL PROLIFERATION; FAMILIAL CANCER; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; SENESCENCE; SKIN FIBROBLAST;

CELL AGING; CYCLIN-DEPENDENT KINASE INHIBITOR P21; CYCLINS; FIBROBLASTS; HUMANS; LI-FRAUMENI SYNDROME; TUMOR SUPPRESSOR PROTEIN P53;

EID: 0029818858     PISSN: 00085472     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (20)

1. Hayflick, L., and Moorhead, P S. The serial cultivation of human diploid cell strains. Exp. Cell Res., 25: 585-621, 1961.
2. Cristofalo, V. J., and Pignolo, R. Replicative senescence of human fibroblast-like cells in culture. Physiol Rev , 73 617-638, 1993.
3. Noda, A., Ning, Y , Venable, S. F., Pereira-Smith, O. M., and Smith, J. R. Cloning of senescent cell-derived inhibitors of DNA synthesis using an expression screen. Exp Cell Res., 211 90-98, 1994.
4. Harvey, M., Sands, A. T., Weiss, R. S., Hegi, M E , Wiseman, R. W , Pantazis, P., Giovanella, B. C., Tainsky, M A , Bradley, A., and Donehower, L A. In vitro growth characteristics of embryo fibroblasts isolated from p53-deficient mice. Oncogene, 8: 2457-2467, 1993.
5. Atadia, P., Wong, H , Garkavtsev, I , Veillette, C , and Riabowol, K. Increased activity of p53 in senescing fibroblasts. Proc. Natl. Acad. Sci. USA, 92: 8348-8352, 1995.
6. Weinberg, R. A. The retinoblastoma protein and cell cycle control. Cell, 81: 323-330, 1995
7. Newbold, R F , Overell, R W., and Connell, J. R. Induction of immortality is an early event in malignant transformation of mammalian cells by carcinogens. Nature (Lond.), 299, 633-634, 1982.
8. Sasaki, M , Honda, T , Yamada, H., Wake, N , Barrett, J C , and Oshimura, M Evidence for multiple pathways to cellular senescence. Cancer Res., 54: 6090-6093, 1994.
9. Malkin, D., Li, F. P., Strong, L. C., Fraumeni, J. F., Jr., Nelson, C. E., Kim, D. H , Kassel, J., Gryka, M A., Bischoff, F Z., Tainsky, M. A , and Friend, S. H Germ line p53 mutations in a familial syndrome of breast cancer, sarcomas, and other neoplasms. Science (Washington DC), 250: 1233-1238, 1990.
10. Bischoff, F. Z., Yim, S. O., Pathak, S., Grant, G., Siciliano, M. J., Giovanella, B. C., Strong, L C., and Tainsky, M. A. Spontaneous abnormalities in normal fibroblasts from patients with Li-Fraumeni cancer syndrome: aneuploidy and immortalization. Cancer Res , 50: 7979-7984, 1990.
11. 1-induced immortalization of cultured skin fibroblasts from a patient with Li-Fraumeni syndrome. Carcinogenesis (Lond.), 16: 25-34, 1995.
12. INK4 expression and telomere length during spontaneous immortalization of Li-Fraumeni syndrome fibroblasts. Mol. Cell Biol., 15: 4745-4753, 1995
13. Cip1 in muscle and other terminally differentiating cells. Science (Washington DC), 267-1024-1027, 1995.
14. Medcalf, A. S C., and Wade, M. H. Comparison of mutagenicity of N-methyl-N-nitrosourea and N-ethyl-N-nitrosourea in human diploid fibroblasts. Carcinogenesis (Lond.), 4: 115-118, 1983.
15. Yin Y., Tainsky, M. A , Bischoff, F. Z., Strong, L. C., and Wahl, G. M. Wild-type p53 restores cell cycle control and inhibits gene amplification in cells with mutant p53 alleles. Cell, 70: 937-948, 1992
16. Cristofalo, V. J , and Scharf, B. B Cellular senescence and DNA synthesis Exp. Cell Res, 76: 419-427, 1973
17. Goodrow, T. L., Storer, R. D., Leander, K. R , Prahalada, S. R., van Zwieten, M. J., and Bradley, M. Murine p53 intron sequences 5-8 and their use in polymerase chain reaction/direct sequencing analysis of p53 mutations in CD-1 mouse liver and lung tumors. Mol. Carcinog., 5: 9-15, 1992.
18. Higuchi, R. Rapid, efficient DNA extraction for PCR from cells or blood. Amplifications - A Forum for PCR Users, Issue 2, pp. 1-3. Norwalk, CT: Perkin Elmer-Cetus, 1989.
19. Chomczynski, P , and Sacchi, N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem., 162: 156-159, 1987.
20. Bischoff, F. Z., Strong, L. C., Yim, S. O., Pratt, D. R , Siciliano, M J., Giovanella, B. C., and Tainsky, M. A. Tumorigenic transformation of spontaneously immortalized fibroblasts from patients with a familial cancer syndrome. Oncogene, 6: 183-186, 1991