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The Drosophila hedgehog gene is expressed specifically in posterior compartment cells and is a target of engrailed regulation
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Inserting the Ftz homeodomain into engrailed creates a dominant transcriptional repressor that specifically turns off Ftz target genes in vivo
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John, A.1
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The TGFβ homolog Dpp and the segment polarity gene hedgehog are required for the propagation of a morphogenetic wave in the Drosophila retina
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Heberlein, U.1
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The segment polarity gene hedgehog is required for progression of the morphogenetic furrow in the developing Drosophila eye
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Ma, C.1
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Sequential organizing activities of engrailed, hedgehog and decapentaplegic in the Drosophila wing
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of special interest. The authors use the flp-out method to ectopically express en, hh, or dpp in clones of cells during wing development. en-expressing clones in the anterior wing behave like supernumerary posterior compartments: en programs posterior cells to express hh, but at the same time it renders these cells unresponsive to this signal. Hh induces at short range the expression of dpp. Dpp can exert a long-range organizing influence, resulting in posterior or anterior structures, depending on the state of en expression in its target cells.
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Zecca M, Basler K, Struhl G. Sequential organizing activities of engrailed, hedgehog and decapentaplegic in the Drosophila wing. of special interest Development. 121:1995;2265-2278 The authors use the flp-out method to ectopically express en, hh, or dpp in clones of cells during wing development. en-expressing clones in the anterior wing behave like supernumerary posterior compartments: en programs posterior cells to express hh, but at the same time it renders these cells unresponsive to this signal. Hh induces at short range the expression of dpp. Dpp can exert a long-range organizing influence, resulting in posterior or anterior structures, depending on the state of en expression in its target cells.
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(1995)
Development
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Zecca, M.1
Basler, K.2
Struhl, G.3
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11
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0028864433
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Creating a Drosophila wing de novo, the role of engrailed, and the compartment boundary hypothesis
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of special interest. The authors succeeded in generating large enlinvected mutant clones that transform posterior cells to an anterior fate and create new A/P compartment boundaries that, in turn, organize pattern in surrounding cells.
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Tabata T, Schwartz C, Gustavson E, Ali Z, Komberg TB. Creating a Drosophila wing de novo, the role of engrailed, and the compartment boundary hypothesis. of special interest Development. 121:1995;3359-3369 The authors succeeded in generating large enlinvected mutant clones that transform posterior cells to an anterior fate and create new A/P compartment boundaries that, in turn, organize pattern in surrounding cells.
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(1995)
Development
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Tabata, T.1
Schwartz, C.2
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Ali, Z.4
Komberg, T.B.5
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12
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0028880509
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The function of engrailed and the specification of Drosophila wing pattern
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of special interest. One of several studies (see e.g., [10,13]) that examines the function of en by ectopic expression. The authors made the surprising observation that experimental overexpression of en in posterior cells inactivates the expression of the endogenous en gene, resulting in a transformation to anterior pattern.
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Guillen I, Mullor JL, Capdevila J, Sanchez-Herrero E, Morata G, Guerrero I. The function of engrailed and the specification of Drosophila wing pattern. of special interest Development. 121:1995;3447-3456 One of several studies (see e.g., [10,13]) that examines the function of en by ectopic expression. The authors made the surprising observation that experimental overexpression of en in posterior cells inactivates the expression of the endogenous en gene, resulting in a transformation to anterior pattern.
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(1995)
Development
, vol.121
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Guillen, I.1
Mullor, J.L.2
Capdevila, J.3
Sanchez-Herrero, E.4
Morata, G.5
Guerrero, I.6
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13
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Distinguishable functions for engrailed and invected in anterior-posterior patterning in the Drosophila wing
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Simmonds AJ, Brook WJ, Cohen SM, Bell JB. Distinguishable functions for engrailed and invected in anterior-posterior patterning in the Drosophila wing. Nature. 376:1995;424-427.
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Nature
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Simmonds, A.J.1
Brook, W.J.2
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14
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Drawing a stripe in Drosophila imaginal discs: Negative regulation of decapentaplegic and patched expression by engrailed
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of special interest. Clones null mutant for enlinvected autonomously express dpp and ptc, suggesting that en might directly act as a negative regulator of these genes. Indeed, the authors identify en binding sites in a regulatory region of the dpp gene.
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Sanicola M, Sekelsky J, Elson S, Gelbart WM. Drawing a stripe in Drosophila imaginal discs: negative regulation of decapentaplegic and patched expression by engrailed. of special interest Genetics. 139:1995;745-756 Clones null mutant for enlinvected autonomously express dpp and ptc, suggesting that en might directly act as a negative regulator of these genes. Indeed, the authors identify en binding sites in a regulatory region of the dpp gene.
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(1995)
Genetics
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Sanicola, M.1
Sekelsky, J.2
Elson, S.3
Gelbart, W.M.4
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15
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0029896212
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Sending and receiving the Hedgehog signal: Control by the Drosophila Gli protein Cubitus interruptus
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of outstanding interest. Clones mutant for a ci null mutation, which are technically very difficult to generate, are described. The surprising outcomes is that these clones express hh, indicating that one of the functions of Ci is to represss hh expression in anterior wing cells. High levels of Ci can activate Hh target genes such a dpp, indicating that Ci functions to transduce the Hh signal.
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Dominguez M, Brunner M, Hafen E, Basler K. Sending and receiving the Hedgehog signal: control by the Drosophila Gli protein Cubitus interruptus. of outstanding interest Science. 272:1996;1621-1625 Clones mutant for a ci null mutation, which are technically very difficult to generate, are described. The surprising outcomes is that these clones express hh, indicating that one of the functions of Ci is to represss hh expression in anterior wing cells. High levels of Ci can activate Hh target genes such a dpp, indicating that Ci functions to transduce the Hh signal.
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Science
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Dominguez, M.1
Brunner, M.2
Hafen, E.3
Basler, K.4
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0029027783
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Analysis of cubitus interruptus regulation in Drosophila embryos and imaginal discs
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of special interest. A demonstration that the anterior-specific expression of ci is the result of direct transcriptional repression of ci by En in posterior cells. These results provide the molecular proof for a long-standing theory that the adult phenotypes of recessive ci alleles are caused by the lack of En repression.
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Schwartz C, Locke J, Nishida C, Komberg TB. Analysis of cubitus interruptus regulation in Drosophila embryos and imaginal discs. of special interest Development. 121:1995;1625-1635 A demonstration that the anterior-specific expression of ci is the result of direct transcriptional repression of ci by En in posterior cells. These results provide the molecular proof for a long-standing theory that the adult phenotypes of recessive ci alleles are caused by the lack of En repression.
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(1995)
Development
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Schwartz, C.1
Locke, J.2
Nishida, C.3
Komberg, T.B.4
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18
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0030602796
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The Drosophila smoothened gene encodes a seven-pass membrane protein, a putative receptor for the Hedgehog signal
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of outstanding interest. The authors describe the cloning of the smo segment polarity gene. They also show that smo is required for Hh signaling in the embryo and that smo loss-of-function is epistatic to ptc loss-of-function. On the basis of genetic evidence, the authors propose that Smo is the Hh receptor and that Ptc acts as an accessory protein.
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Alcedo J, Ayzenzon M, Von Ohlen T, Noll M, Hooper JE. The Drosophila smoothened gene encodes a seven-pass membrane protein, a putative receptor for the Hedgehog signal. of outstanding interest Cell. 86:1996;221-232 The authors describe the cloning of the smo segment polarity gene. They also show that smo is required for Hh signaling in the embryo and that smo loss-of-function is epistatic to ptc loss-of-function. On the basis of genetic evidence, the authors propose that Smo is the Hh receptor and that Ptc acts as an accessory protein.
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(1996)
Cell
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Alcedo, J.1
Ayzenzon, M.2
Von Ohlen, T.3
Noll, M.4
Hooper, J.E.5
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19
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0029784223
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Smoothened encodes a receptor-like serpentine protein required for hedgehog signaling
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of outstanding interest. The second smo cloning paper (see [18]). These authors use a similar approach to show that smo is needed for Hh signaling. They also describe smo mutant clones that lose Hh-dependent dpp expression in cells near the A/P compartment boundary of the wing disc, providing further evidence of Smo's role in Hh signaling.
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Van den Heuvel M, Ingham PW. Smoothened encodes a receptor-like serpentine protein required for hedgehog signaling. of outstanding interest Nature. 382:1996;547-551 The second smo cloning paper (see [18]). These authors use a similar approach to show that smo is needed for Hh signaling. They also describe smo mutant clones that lose Hh-dependent dpp expression in cells near the A/P compartment boundary of the wing disc, providing further evidence of Smo's role in Hh signaling.
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(1996)
Nature
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Van Den Heuvel, M.1
Ingham, P.W.2
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The Drosophila patched gene encodes a putative membrane protein required for segmental patterning
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Hooper JE, Scott MP. The Drosophila patched gene encodes a putative membrane protein required for segmental patterning. Cell. 59:1989;751-765.
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Cell
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Hooper, J.E.1
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Role of the Drosophila patched gene in positional signaling
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Ingham PW, Taylor AM, Nakono Y. Role of the Drosophila patched gene in positional signaling. Nature. 353:1991;184-187.
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Nature
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Ingham, P.W.1
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A protein with several possible membrane-spanning domains encoded by the Drosophila segment polarity gene patched
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Nakano Y, Guerrero I, Hidalgo A, Taylor A, Whittle JR, Ingham PW. A protein with several possible membrane-spanning domains encoded by the Drosophila segment polarity gene patched. Nature. 341:1989;508-513.
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Nakano, Y.1
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Taylor, A.4
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0028918468
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Protein kinase A and Hedgehog signaling in Drosophila limb development
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Jiang J, Struhl G. Protein kinase A and Hedgehog signaling in Drosophila limb development. Cell. 80:1995;563-572.
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Cell
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Jiang, J.1
Struhl, G.2
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0028952718
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Signal transduction by cAMP-dependent protein kinase A in Drosophila limb patterning
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Lepage T, Cohen SM, Diaz-Benjumea FJ, Parkhurst SM. Signal transduction by cAMP-dependent protein kinase A in Drosophila limb patterning. Nature. 373:1995;656-657.
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Nature
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Lepage, T.1
Cohen, S.M.2
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0028897883
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Li W, Ohlmeyer JT, Lane ME, Kalderon D. Function of protein kinase A in hedgehog signal transduction and Drosophila imaginal disc development. Cell. 80:1995;553-562.
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Cell
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Li, W.1
Ohlmeyer, J.T.2
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Kalderon, D.4
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26
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0028911915
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CAMP-dependent protein kinase and hedgehog act antagonistically in regulating decapentaplegic transcription in Drosophila imaginal discs
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Pan D, Rubin GM. cAMP-dependent protein kinase and hedgehog act antagonistically in regulating decapentaplegic transcription in Drosophila imaginal discs. Cell. 80:1995;543-552.
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Pan, D.1
Rubin, G.M.2
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27
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0030297553
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Dual roles for Patched in sequestering and transducing Hedgehog
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of outstanding interest. Using elegant genetic experiments, the authors demonstrate that Hh binds to Ptc. They also address the long-standing issue of why Ptc is upregulated in cells receiving the Hh signal: if this upregulation is prevented, the Hh signal spreads too far into the anterior compartment. Thus, the high levels of Ptc induced by Hh serve to restrict the movement and range of the Hh signal.
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Chen Y, Struhl G. Dual roles for Patched in sequestering and transducing Hedgehog. of outstanding interest Cell. 87:1996;553-564 Using elegant genetic experiments, the authors demonstrate that Hh binds to Ptc. They also address the long-standing issue of why Ptc is upregulated in cells receiving the Hh signal: if this upregulation is prevented, the Hh signal spreads too far into the anterior compartment. Thus, the high levels of Ptc induced by Hh serve to restrict the movement and range of the Hh signal.
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(1996)
Cell
, vol.87
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Chen, Y.1
Struhl, G.2
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28
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0029948270
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Biochemical evidence that Patched is the Hedgehog receptor
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of special interest. Xenopus oocytes injected with ptc mRNA bind recombinant Shh protein, whereas control oocytes, or injected oocytes in the presence of a blocking antibody, do not. These results strongly suggest that Ptc is the Hh receptor.
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Marigo V, Davey RA, Zuo Y, Cunningham JM, Tabin CJ. Biochemical evidence that Patched is the Hedgehog receptor. of special interest Nature. 384:1996;176-179 Xenopus oocytes injected with ptc mRNA bind recombinant Shh protein, whereas control oocytes, or injected oocytes in the presence of a blocking antibody, do not. These results strongly suggest that Ptc is the Hh receptor.
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(1996)
Nature
, vol.384
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Marigo, V.1
Davey, R.A.2
Zuo, Y.3
Cunningham, J.M.4
Tabin, C.J.5
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29
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0011102935
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The tumour suppressor gene patched encodes a candidate receptor for Sonic hedgehog
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of special interest. Isolation of a vertebrate Smo homolog and biochemical evidence that it does not bind Sonic Hedgehog (Shh). However, Ptc protein can bind Shh, indicating that Ptc might be the Shh receptor. Moreover, Ptc binds to Smo, raising the possibility that non-Shh-bound Ptc inhibits Smo activity in a multi-subunit Shh receptor complex.
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Stone DM, Hynes M, Armanini M, Swanson TA, Gu Q, Johnson RL, Scott MP, Pennica D, Goddard A, Phillips H. The tumour suppressor gene patched encodes a candidate receptor for Sonic hedgehog. of special interest Nature. 384:1996;129-134 Isolation of a vertebrate Smo homolog and biochemical evidence that it does not bind Sonic Hedgehog (Shh). However, Ptc protein can bind Shh, indicating that Ptc might be the Shh receptor. Moreover, Ptc binds to Smo, raising the possibility that non-Shh-bound Ptc inhibits Smo activity in a multi-subunit Shh receptor complex.
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(1996)
Nature
, vol.384
, pp. 129-134
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Stone, D.M.1
Hynes, M.2
Armanini, M.3
Swanson, T.A.4
Gu, Q.5
Johnson, R.L.6
Scott, M.P.7
Pennica, D.8
Goddard, A.9
Phillips, H.10
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30
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0029417009
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Patched overexpression alters wing disc size and pattern: Transcriptional and post-transcriptional effects on hedgehog targets
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of special interest. Hh induces transcription of target genes (e.g. dpp and ptc) by opposing the Ptc signaling pathway. By the same route, Hh represses the accumulation of Ci protein. The authors overexpressed ptc and found that this impedes the transcription of dpp and ptc and the post-transcriptional accumulation of Ci.
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Johnson RL, Grenier JK, Scott MP. Patched overexpression alters wing disc size and pattern: transcriptional and post-transcriptional effects on hedgehog targets. of special interest Development. 121:1995;4161-4170 Hh induces transcription of target genes (e.g. dpp and ptc) by opposing the Ptc signaling pathway. By the same route, Hh represses the accumulation of Ci protein. The authors overexpressed ptc and found that this impedes the transcription of dpp and ptc and the post-transcriptional accumulation of Ci.
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(1995)
Development
, vol.121
, pp. 4161-4170
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Johnson, R.L.1
Grenier, J.K.2
Scott, M.P.3
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31
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0030027059
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Conservation of the hedgehog/patched signaling pathway from files to mice: Induction of a mouse patched gene by Hedgehog
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of special interest. This group has identified a mouse homolog of the Drosophila ptc gene. This gene is expressed near Sonic and Indian Hh producing cells wherever the authors looked. It is a fantastic reagent to visualize the range and target sites of functional Hh signaling.
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Goodrich LV, Johnson RL, Milenkovic L, McMahon JA, Scott MP. Conservation of the hedgehog/patched signaling pathway from files to mice: induction of a mouse patched gene by Hedgehog. of special interest Genes Dev. 10:1996;301-312 This group has identified a mouse homolog of the Drosophila ptc gene. This gene is expressed near Sonic and Indian Hh producing cells wherever the authors looked. It is a fantastic reagent to visualize the range and target sites of functional Hh signaling.
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(1996)
Genes Dev
, vol.10
, pp. 301-312
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Goodrich, L.V.1
Johnson, R.L.2
Milenkovic, L.3
McMahon, J.A.4
Scott, M.P.5
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32
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0029879687
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Protein kinase A is a common negative regulator of Hedgehog signaling in the vertebrate embryo
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of special interest. Injection of mRNAs encoding dominant-negative and constitutively activated forms of PKA result in similar phenotypes as those encoding Sonic or Indian Hh. PKA is a negative regulator of Hh signaling also in vertebrates.
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Hammerschmidt M, Bitgood MJ, McMahon AP. Protein kinase A is a common negative regulator of Hedgehog signaling in the vertebrate embryo. of special interest Genes Dev. 10:1996;647-658 Injection of mRNAs encoding dominant-negative and constitutively activated forms of PKA result in similar phenotypes as those encoding Sonic or Indian Hh. PKA is a negative regulator of Hh signaling also in vertebrates.
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(1996)
Genes Dev
, vol.10
, pp. 647-658
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Hammerschmidt, M.1
Bitgood, M.J.2
McMahon, A.P.3
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33
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0029975823
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Conservation of hedgehog signalling: Induction of a chicken patched homolog by Sonic hedgehog in the developing limb
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of special interest. The chick homolog of ptc, like the mouse homolog, is expressed in CNS and limb development in and around Shh-expressing cells. Ectopic expression of Shh causes ectopic ptc expression, suggesting that the relationship between Shh and Ptc is the same as that between their Drosophila homologs.
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Marigo V, Scott MP, Johnson RL, Goodrich LV, Tabin CJ. Conservation of hedgehog signalling: induction of a chicken patched homolog by Sonic hedgehog in the developing limb. of special interest Development. 122:1996;1225-1233 The chick homolog of ptc, like the mouse homolog, is expressed in CNS and limb development in and around Shh-expressing cells. Ectopic expression of Shh causes ectopic ptc expression, suggesting that the relationship between Shh and Ptc is the same as that between their Drosophila homologs.
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(1996)
Development
, vol.122
, pp. 1225-1233
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Marigo, V.1
Scott, M.P.2
Johnson, R.L.3
Goodrich, L.V.4
Tabin, C.J.5
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34
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Inhibition of protein kinase A phenocopies ectopic expression of hedgehog in the CNS of wild-type and cyclops mutant embryos
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Ungar AR, Moon RT. Inhibition of protein kinase A phenocopies ectopic expression of hedgehog in the CNS of wild-type and cyclops mutant embryos. Dev Biol. 178:1996;186-191.
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Dev Biol
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Ungar, A.R.1
Moon, R.T.2
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35
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0029858774
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Transcriptional activation of hedgehog target genes in Drosophila is mediated directly by the Cubitus interruptus protein, a member of the GLI family of zinc finger DNA-binding proteins
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of special interest. Elevated levels of Ci are sufficient to activate ptc and dpp expression in the absence of Hh activity. Moreover, the authors identify a Gli consensus binding site in the ptc gene that is necessary to direct reporter gene expression in response to Hh signaling. This provides strong evidence that the activation of ptc (and presumably dpp) transcription by Ci is direct.
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Alexandre C, Jacinto A, Ingham PW. Transcriptional activation of hedgehog target genes in Drosophila is mediated directly by the Cubitus interruptus protein, a member of the GLI family of zinc finger DNA-binding proteins. of special interest Genes Dev. 10:1996;2003-2013 Elevated levels of Ci are sufficient to activate ptc and dpp expression in the absence of Hh activity. Moreover, the authors identify a Gli consensus binding site in the ptc gene that is necessary to direct reporter gene expression in response to Hh signaling. This provides strong evidence that the activation of ptc (and presumably dpp) transcription by Ci is direct.
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(1996)
Genes Dev
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Alexandre, C.1
Jacinto, A.2
Ingham, P.W.3
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Molecular analysis of cubitus interruptus (ci) mutations suggests an explanation for the unusual ci position effects
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Mutations that alter the timing and pattern of cubitus interruptus gene expression in Drosophila melanogaster
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Cell, which generate altered protein products that are associated with gain- and loss-of-function phenotypes, respectively.
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Cell, which generate altered protein products that are associated with gain- and loss-of-function phenotypes, respectively.
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Basler K, Struhl G. Compartment boundaries and the control of Drosophila limb pattern by Hedgehog protein. Nature. 368:1994;208-214.
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The Drosophila segment polarity gene patched interacts with decapentaplegic in wing development
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Capdevilla J, Estrada MP, Sanchez-Herrero E, Guerrero I. The Drosophila segment polarity gene patched interacts with decapentaplegic in wing development. EMBO J. 13:1994;17-28.
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Quantitative effects of hedgehog and decapentaplegic activity on the patterning of the Drosophila wing
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of special interest. One of several studies (see e.g. [10,43]) demonstrating that ectopic expression of dpp alone is sufficient to induce pattern alterations similar to those caused by ectopic hh activity.
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Ingham PW, Fietz MJ. Quantitative effects of hedgehog and decapentaplegic activity on the patterning of the Drosophila wing. of special interest Curr Biol. 5:1995;432-440 One of several studies (see e.g. [10,43]) demonstrating that ectopic expression of dpp alone is sufficient to induce pattern alterations similar to those caused by ectopic hh activity.
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Ingham, P.W.1
Fietz, M.J.2
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0028178786
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Hedgehog is a signaling protein with a key role in patterning Drosophila imaginal discs
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Tabata T, Kornberg TB. Hedgehog is a signaling protein with a key role in patterning Drosophila imaginal discs. Cell. 76:1994;89-102.
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Tabata, T.1
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0027983638
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Targeted expression of the signaling molecule Decapentaplegic induces pattern duplications and growth alterations in Drosophila wings
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Capdevila J, Guerrero I. Targeted expression of the signaling molecule Decapentaplegic induces pattern duplications and growth alterations in Drosophila wings. EMBO J. 13:1994;4459-4468.
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Direct and long-range action of a Wingless morphogen gradient
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Zecca M, Basler K, Struhl G. Direct and long-range action of a Wingless morphogen gradient. Cell. 87:1996;833-844.
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Cell interaction between compartments establishes the proximal-distal axis of Drosophila legs
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Diaz-Benjumea FJ, Cohen B, Cohen SM. Cell interaction between compartments establishes the proximal-distal axis of Drosophila legs. Nature. 372:1994;175-179.
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Cohen, S.M.3
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46
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Complementary and mutually exclusive activities of Decapentaplegic and Wingless organize axial patterning during Drosophila leg development
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of outstanding interest. By describing the effects of single and double mutant clones of various Hh, Wg and Dpp signal components, the authors provide compelling evidence of an antagonistic interaction between Wg and Dpp in the leg disc, with Dpp repressing wg expression in dorsal cells, and vice versa in ventral cells. This circuit ensures the spatial expression pattern of these three signaling molecules that govern leg patterning.
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Jiang J, Struhl G. Complementary and mutually exclusive activities of Decapentaplegic and Wingless organize axial patterning during Drosophila leg development. of outstanding interest Cell. 86:1996;401-409 By describing the effects of single and double mutant clones of various Hh, Wg and Dpp signal components, the authors provide compelling evidence of an antagonistic interaction between Wg and Dpp in the leg disc, with Dpp repressing wg expression in dorsal cells, and vice versa in ventral cells. This circuit ensures the spatial expression pattern of these three signaling molecules that govern leg patterning.
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Cell
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Jiang, J.1
Struhl, G.2
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47
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0029795757
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Antagonistic interactions between Wingless and Decapentaplegic responsible for dorsal-ventral pattern in the Drosophila leg
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of outstanding interest. With a slightly different experimental approach, the authors come to similar conclusion as [46], this time using reduction of endogenous Dpp or Wg activity and ectopic expression of Dpp and Wg to demonstrate their mutual repressive effects. The authors argue that these antagonistic interactions provide a stable circuitry for dorsoventral patterning that is not based on lineage compartments.
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Brook WJ, Cohen SM. Antagonistic interactions between Wingless and Decapentaplegic responsible for dorsal-ventral pattern in the Drosophila leg. of outstanding interest Science. 273:1996;1373-1377 With a slightly different experimental approach, the authors come to similar conclusion as [46], this time using reduction of endogenous Dpp or Wg activity and ectopic expression of Dpp and Wg to demonstrate their mutual repressive effects. The authors argue that these antagonistic interactions provide a stable circuitry for dorsoventral patterning that is not based on lineage compartments.
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Science
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Brook, W.J.1
Cohen, S.M.2
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48
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0029901698
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Decapentaplegic restricts the domain of wingless during Drosophila limb patterning
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of outstanding interest. It is shown that Hh-receiving cells in the anterior compartment that lack punt or tkv activity transcribe wg. Therefore, Dpp signaling normally prevents wg expression in dorsal leg cells and also in distal regions of the wing. (See also [46,47].)
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Penton A, Hoffmann FM. Decapentaplegic restricts the domain of wingless during Drosophila limb patterning. of outstanding interest Nature. 382:1996;162-165 It is shown that Hh-receiving cells in the anterior compartment that lack punt or tkv activity transcribe wg. Therefore, Dpp signaling normally prevents wg expression in dorsal leg cells and also in distal regions of the wing. (See also [46,47].).
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(1996)
Nature
, vol.382
, pp. 162-165
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Penton, A.1
Hoffmann, F.M.2
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49
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0030578403
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Decapentaplegic overexpression affects Drosophila wing and leg imaginal disc development and wingless expression
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Morimura S, Maves L, Chen Y, Hoffmann FM. Decapentaplegic overexpression affects Drosophila wing and leg imaginal disc development and wingless expression. Dev Biol. 177:1996;136-151.
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Morimura, S.1
Maves, L.2
Chen, Y.3
Hoffmann, F.M.4
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50
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0027453804
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Axis specification in the developing Drosophila appendage: The role of wingless, decapentaplegic, and the homeobox gene aristaless
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Campbell G, Weaver T, Tomlinson A. Axis specification in the developing Drosophila appendage: the role of wingless, decapentaplegic, and the homeobox gene aristaless. Cell. 74:1993;1113-1123.
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Campbell, G.1
Weaver, T.2
Tomlinson, A.3
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51
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0029022144
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Wingless and patched are negative regulators of the morphogenetic furrow and can affect tissue polarity in the developing Drosophila compound eye
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of special interest. Removing wg function in the eye disc results in ectopic morphogenetic furrow initiation, indicating that Wg is normally required to inhibit initiation at the lateral margins. The authors then use ectopic furrows to investigate the mechanism of cluster rotation in the eye.
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Ma C, Moses K. Wingless and patched are negative regulators of the morphogenetic furrow and can affect tissue polarity in the developing Drosophila compound eye. of special interest Development. 121:1995;2279-2289 Removing wg function in the eye disc results in ectopic morphogenetic furrow initiation, indicating that Wg is normally required to inhibit initiation at the lateral margins. The authors then use ectopic furrows to investigate the mechanism of cluster rotation in the eye.
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(1995)
Development
, vol.121
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Ma, C.1
Moses, K.2
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52
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0028971728
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Wingless inhibits morphogenetic furrow movement in the Drosophila eye disc
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of special interest. The authors show that wg inhibits morphogenetic furrow (MF) movement in the eye disc. They also show that loss of wg results in ectopic MF initiation, and go further to show that ectopic Wg can block endogenous initiation at the posterior margin, without affecting dpp expression.
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Treisman JE, Rubin GM. Wingless inhibits morphogenetic furrow movement in the Drosophila eye disc. of special interest Development. 121:1995;3519-3527 The authors show that wg inhibits morphogenetic furrow (MF) movement in the eye disc. They also show that loss of wg results in ectopic MF initiation, and go further to show that ectopic Wg can block endogenous initiation at the posterior margin, without affecting dpp expression.
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(1995)
Development
, vol.121
, pp. 3519-3527
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Treisman, J.E.1
Rubin, G.M.2
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53
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0030037438
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Mad acts downstream of Dpp receptors, revealing a differential requirement for dpp signaling in initiation and propagation of morphogenesis in the Drosophila eye
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of special interest. The authors use a mutation in Mad to compromise Dpp signal transduction in eye cells and find that this primarily affects morphogenetic furrow initiation, but not is subsequent propagation. Moreover, their analysis of Mad mutant clones reveals that Dpp signaling is necessary to repress wg expression, reminiscent of the situation in the leg disc (see [46-48]).
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Wiersdorff V, Lecuit T, Cohen SM, Mlodzik M. Mad acts downstream of Dpp receptors, revealing a differential requirement for dpp signaling in initiation and propagation of morphogenesis in the Drosophila eye. of special interest Development. 122:1996;2153-2162 The authors use a mutation in Mad to compromise Dpp signal transduction in eye cells and find that this primarily affects morphogenetic furrow initiation, but not is subsequent propagation. Moreover, their analysis of Mad mutant clones reveals that Dpp signaling is necessary to repress wg expression, reminiscent of the situation in the leg disc (see [46-48]).
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(1996)
Development
, vol.122
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Wiersdorff, V.1
Lecuit, T.2
Cohen, S.M.3
Mlodzik, M.4
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54
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Growth and differentiation in the Drosophila eye coordinated by hedgehog
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Heberlein U, Singh CM, Luk AY, Donohoe TJ. Growth and differentiation in the Drosophila eye coordinated by hedgehog. Nature. 373:1995;709-711.
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(1995)
Nature
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Heberlein, U.1
Singh, C.M.2
Luk, A.Y.3
Donohoe, T.J.4
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55
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0028906799
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Regulation of furrow progression in the Drosophila eye by cAMP-dependent protein kinase A
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Strutt DI, Wiersdorff V, Mlodzik M. Regulation of furrow progression in the Drosophila eye by cAMP-dependent protein kinase A. Nature. 373:1995;705-709.
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Nature
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Strutt, D.I.1
Wiersdorff, V.2
Mlodzik, M.3
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56
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0030297378
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Hedgehog dependent patterning in the Drosophila eye can occur in the absence of Dpp signaling
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of special interest. Using tkv and punt mutant clones (tkv and punt encode the Dpp type I and type II receptors) and large dpp null clones, the authors demonstrate that dpp is not required for the Hh-mediated patterning events in the eye: namely, progression of the morphogenetic furrow and subsequent neuronal differentiation. This is a surprising result and indicates Hh must act via mechanisms other than the induction of dpp in this tissue.
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Burke R, Basler K. Hedgehog dependent patterning in the Drosophila eye can occur in the absence of Dpp signaling. of special interest Dev Biol. 179:1996;360-368 Using tkv and punt mutant clones (tkv and punt encode the Dpp type I and type II receptors) and large dpp null clones, the authors demonstrate that dpp is not required for the Hh-mediated patterning events in the eye: namely, progression of the morphogenetic furrow and subsequent neuronal differentiation. This is a surprising result and indicates Hh must act via mechanisms other than the induction of dpp in this tissue.
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(1996)
Dev Biol
, vol.179
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Burke, R.1
Basler, K.2
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57
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0028940853
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Genetic characterization and cloning of Mothers against dpp, a gene required for decapentaplegic function in Drosophila melanogaster
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Sekelsky JJ, Newfeld SJ, Raftery LA, Chartoff EH, Gelbart WM. Genetic characterization and cloning of Mothers against dpp, a gene required for decapentaplegic function in Drosophila melanogaster. Genetics. 139:1995;1347-1358.
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Genetics
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Sekelsky, J.J.1
Newfeld, S.J.2
Raftery, L.A.3
Chartoff, E.H.4
Gelbart, W.M.5
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58
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0029894568
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Direct and long-range action of a Dpp morphogen gradient
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QD expression demonstrates that Dpp acts directly at a distance to control target gene expression. All omb-expressing cells continuously require tkv activity to express omb, even late in development and even if they are situated far away from the Dpp source. This finding argues against the 'cellular memory' mechanism (see [59]).
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QD expression demonstrates that Dpp acts directly at a distance to control target gene expression. All omb-expressing cells continuously require tkv activity to express omb, even late in development and even if they are situated far away from the Dpp source. This finding argues against the 'cellular memory' mechanism (see [59]).
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(1996)
Cell
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Nellen, D.1
Burke, R.2
Struhl, G.3
Basler, K.4
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59
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0029967209
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Two distinct mechanisms for long-range patterning by Decapentaplegic in the Drosophila wing
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of outstanding interest. The authors describe two distinct mechanisms for long-range patterning by Decapentaplegic in the Drosophila wing. They also show that the induction of dpp target genes does not involve secondary signals (see [58]). However, they argue that Dpp does not act over a long range. According to their 'cellular memory' model, cells that are exposed to a short-range Dpp signal early during development would remember this, and their descendants would continue to express omb, even if they have moved out of the range of the Dpp signal because of disc growth.
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Lecuit T, Brook WJ, Ng M, Calleja M, Sun H, Cohen SM. Two distinct mechanisms for long-range patterning by Decapentaplegic in the Drosophila wing. of outstanding interest Nature. 381:1996;387-393 The authors describe two distinct mechanisms for long-range patterning by Decapentaplegic in the Drosophila wing. They also show that the induction of dpp target genes does not involve secondary signals (see [58]). However, they argue that Dpp does not act over a long range. According to their 'cellular memory' model, cells that are exposed to a short-range Dpp signal early during development would remember this, and their descendants would continue to express omb, even if they have moved out of the range of the Dpp signal because of disc growth.
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(1996)
Nature
, vol.381
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Lecuit, T.1
Brook, W.J.2
Ng, M.3
Calleja, M.4
Sun, H.5
Cohen, S.M.6
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60
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0029979608
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Control of the gene optomotor blind in Drosophila wing development by decapentaplegic and wingless
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of outstanding interest. omb mutants were originally identified in Heisenberg's lab on the basis of a behavioral defect [64]. Pflugfelder et al. [65] cloned the gene and found that it encodes a T-box protein similar to the activin target gene Brachyury. In this paper, Grimm and Pflugfelder show that omb expression in imaginal discs is controlled by Dpp, suggesting that other T-box genes might also be targets for signals of the TGFβ superfamily.
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Grimm S, Pflugfelder GO. Control of the gene optomotor blind in Drosophila wing development by decapentaplegic and wingless. of outstanding interest Science. 271:1996;1601-1604 omb mutants were originally identified in Heisenberg's lab on the basis of a behavioral defect [64]. Pflugfelder et al. [65] cloned the gene and found that it encodes a T-box protein similar to the activin target gene Brachyury. In this paper, Grimm and Pflugfelder show that omb expression in imaginal discs is controlled by Dpp, suggesting that other T-box genes might also be targets for signals of the TGFβ superfamily.
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(1996)
Science
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Grimm, S.1
Pflugfelder, G.O.2
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61
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0029900174
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A gene complex acting downstream of dpp in Drosophila wing morphogenesis
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of special interest. spalt is a target gene of Dpp in the wing. It turns out that there is a neighboring gene very homologous to spalt (called spalt-related) that is expressed in an identical pattern during wing development. The function of spalt and spalt-related are partially redundant and only when both genes are eliminated, is their role in wing morphogenesis revealed.
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De Celis JF, Barrio R, Kafatos FC. A gene complex acting downstream of dpp in Drosophila wing morphogenesis. of special interest Nature. 381:1996;421-424 spalt is a target gene of Dpp in the wing. It turns out that there is a neighboring gene very homologous to spalt (called spalt-related) that is expressed in an identical pattern during wing development. The function of spalt and spalt-related are partially redundant and only when both genes are eliminated, is their role in wing morphogenesis revealed.
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(1996)
Nature
, vol.381
, pp. 421-424
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De Celis, J.F.1
Barrio, R.2
Kafatos, F.C.3
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62
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0030018783
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Dpp receptors are autonomously required for cell proliferation in the entire developing Drosophila wing
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of special interest. By looking at the requirement for the Dpp receptors (Tkv and Punt), the authors show that Dpp also controls cell proliferation in imaginal discs. They also present an independent set of experiments demonstrating that Dpp input is required in cells distant from the dpp-expressing cells.
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Burke R, Basler K. Dpp receptors are autonomously required for cell proliferation in the entire developing Drosophila wing. of special interest Development. 122:1996;2261-2269 By looking at the requirement for the Dpp receptors (Tkv and Punt), the authors show that Dpp also controls cell proliferation in imaginal discs. They also present an independent set of experiments demonstrating that Dpp input is required in cells distant from the dpp-expressing cells.
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(1996)
Development
, vol.122
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Burke, R.1
Basler, K.2
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Hairy and emc negatively regulate morphogenetic furrow progression in the Drosophila eye
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Brown NL, Sattler CA, Paddock SW, Carroll SB. Hairy and emc negatively regulate morphogenetic furrow progression in the Drosophila eye. Cell. 80:1995;879-887.
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The use of mutations for the partial degradation of vision in Drosophila melanogaster
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Genetics and molecular characterization of the optomoter-blind gene locus in Drosophila
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Pflugfelder GO, Schwarz H, Roth H, Poeck B, Sigl A, Kerscher S, Jonschker B, Pak WL, Heisenberg M. Genetics and molecular characterization of the optomoter-blind gene locus in Drosophila. Genetics. 126:1990;91-104.
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