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Samples for transmission electron microscopy measurements were prepared by placing 1-2 drops of colloidal nanoparticles onto holey carbon films supported on copper grids. The specimens were allowed to dry for at least 12 h prior to imaging. Samples were examined using a JEOL 100CXII Transmission Electron Microscope operating at 100 kV.
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0347016697
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note
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4, became red, and finally, orange-brown. The reaction was allowed to proceed for 2 h following addition of the reducing agent. The organic phase was then removed under vacuum to isolate the dry dodecanthiolated silver nanoparticles. The isolated particles were purified (to remove unreacted phase transfer reagent and dodecanthiol) by diluting with ethanol (120 mL) followed by suction filtration, yielding a waxy orange-brown solid.
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note
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L = 0.184 ± 0.004 (DMPC and DMPE), with PEG5000 (MW = 5000; n = 113 monomer units) grafted phospholipid to total phospholipid content of 6.5 mol %. Hydration of the solid components was carried out with either water (undoped and dodecanthiol-derivatized Ag particle phases) or Ag hydrosols (bare Ag or LDS-stabilized Ag nanoparticles) by repeated cycles of warming to 35°C, vortexing, and cooling on an ice bath until uniform transparent elastic solid/ gels were formed at room temperature.
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-1 for low q studies, respectively. The area detector images were corrected for background scattering of water by subtracting from the recorded images an area detector image of a water sample obtained with the same total exposure time as for the complex fluid samples (1 s). The collected scattering data were calibrated on the basis of the known positions of silver behenate powder Bragg reflections. All samples were measured within 1 week of preparation.
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