Differential expression of telomerase activity in hematopoietic progenitors from adult human bone marrow

Stem Cells

Volumn 14, Issue 2, 1996, Pages 239-248

  Chiu, Choy Pik ^a   Dragowska, Wieslawa ^b   Kim, Nam Woo ^a   Vaziri, Homayoun ^a   Yui, Jane ^b   Thomas, Terry E ^b   Harley, Calvin B ^a   Lansdorp, Peter M ^b  

^a GERON CORPORATION   (United States)

^b BRITISH COLUMBIA CANCER AGENCY   (Canada)

Author keywords

Hematopoietic progenitors; Human bone marrow; Primitive stem cells; Telomerase; TRAP assay

Indexed keywords

ENZYME; TELOMERASE;

ADOLESCENT; ADULT; ARTICLE; CELL DIVISION; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME INDUCTION; ENZYME LOCALIZATION; HEMATOPOIETIC STEM CELL; HUMAN; HUMAN CELL; MONONUCLEAR CELL; POLYMERASE CHAIN REACTION; TELOMERE;

SIGANUS;

EID: 0029940570     PISSN: 10665099     EISSN: None     Source Type: Journal    
DOI: 10.1002/stem.140239     Document Type: Article

References (34)

1. Blackburn E. Structure and function of telomeres. Nature 1991;350:569-573.
2. Moyzis RK, Buckingham JM, Cram LS et al. A highly conserved repetitive DNA sequence. (TTAGGG), present at the telomeres of human chromosomes. Proc Natl Acad Sci USA 1988;85:6622-6626.
3. Olovnikov A. A theory of marginotomy: the incomplete copying of template margin in enzymatic synthesis of polynucleotides and biological significance of the phenomenon. J Theor Biol 1973;41:181-190.
4. Watson J. Origin of concatemeric T7 DNA. Nature New Biol 1972;239.197-201.
5. Harley C, Futcher A, Greider C. Telomeres shorten during aging of human fibroblasts. Nature 1990;345:458-460.
6. Lindsey J, McGill N, Lindsey L et al. In vivo loss of telomeric repeats with age in humans. Mutat Res 1991;256:45-48.
7. Hastie ND, Dempster M, Dunlop MG et al. Telomere reduction in human colorectal carcinoma and with aging. Nature 1990;346:866-868.
8. Levy MZ, Allsopp RC, Futcher AB et al. Telomere end-replication problem and cell aging. J Mol Biol 1992;225:951-960.
9. Vaziri H, Schachter F, Uchida I et al. Loss of telomeric DNA during aging of normal and trisomy 21 human lymphocytes. Am J Hum Genet 1993;52:661-667.
10. Chang E, Harley C. Telomere length and replicative aging in human vascular tissues. Proc Natl Acad Sci USA 1995;92:11190-11194.
11. Hayflick L. The limited in vitro lifetime of human diploid cell strains. Exp Cell Res 1965;37:614-636.
12. Hayflick L, Moorhead PS. The serial cultivation of human diploid cell strains. Exp Cell Res 1961;25:585-621.
13. Perillo NL, Walford RL, Newman MA et al. Human T lymphocytes possess a limited in vitro life span. Exp Gerontol 1989;24:177-187.
14. Allsopp RC, Vaziri H, Patterson C et al. Telomere length predicts replicative capacity of human fibroblasts. Proc Natl Acad Sci USA 1992;89:10114-10118.
15. Counter C, Avilion A, LeFeuvre C et al. Telomere shortening associated with chromosome instability is arrested in immortal cells which express telomerase activity. EMBO J 1992;11:1921-1929.
16. de Lange T, Shiue L, Myers RM et al. Structure and variability of human chromosome ends. Mol Cell Biol 1990;10:518-527.
17. Greider C, Blackburn E. Identification of a specific telomere terminal transferase activity in tetrahymena extracts. Cell 1985;43:405-413.
18. Morin GB. The human telomere terminal transferase enzyme is a ribonucleoprotein that synthesizes TTAGGG repeats. Cell 1989;59:521-529.
19. Harley C. Telomere loss: mitotic clock or genetic time bomb? Mutat Res 1991;256:271-282.
20. Counter CM, Hirte HW, Bacchetti S et al. Telomerase activity in human ovarian carcinoma. Proc Natl Acad Sci USA 1994;91:2900-2904.
21. Kim NW, Piatyszek MA, Prowse KR et al. Specific association of human telomerase activity with immortal cells and cancer. Science 1994;266:2011-2015.
22. Vaziri H, Dragowska W, Allsopp RC et al. Evidence for a mitotic clock in human hematopoietic stem cells: loss of telomeric DNA with age. Proc Natl Acad Sci USA 1994;91:9857-9860.
23. Moore MA. Hematopoietic reconstruction: new approaches. Clin Cancer Res 1995;1:3-9.
24. Lansdorp PM, Dragowska W. Long-term erythropoiesis from constant numbers of CD34+ cells in serum-free cultures initiated with highly purified progenitor cells from human bone marrow. J Exp Med 1992;175:1501-1509.
25. Lansdorp PM, Dragowska W, Mayani H. Ontogeny-related changes in proliferative potential of human hematopoietic cells. J Exp Med 1993;178:787-791.
26. Counter CM, Gupta J, Harley CB et al. Telomerase activity in normal leukocytes and in hematologic malignancies. Blood 1995;85:2315-2320.
27. Hiyama K, Hirai Y, Kyoizumi S et al. Activation of telomerase in human lymphocytes and hematopoietic progenitor cells. J Immunol 1995;155:3711-3715.
28. Broccoli D, Young JW, de Lange T Telomerase activity in normal and malignant hematopoietic cells. Proc Natl Acad Sci USA 1995;92:9082-9086.
29. Mayani H, Dragowska W, Lansdorp PM. Cytokine-induced selective expansion and maturation of erythroid versus myeloid progenitors from purified cord blood precursor cells. Blood 1993;82:3252-3258.
30. Civin CI, Strauss LC, Brovall C et al. Antigenic analysis of hematopoiesis. III. A hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-1a cells. J Immunol 1984;133:157-165.
31. Zhong Z, Shiue L, Kaplan S et al. A mammalian factor that binds telomeric TTAGGG repeats in vitro. Mol Cell Biol 1992;12:4834-4843.
32. n repeats. Nucleic Acids Res 1992;20:1387-1391.
33. Cardenas ME, Bianchi A, de Lange T. A Xenopus egg factor with DNA-binding properties characteristic of terminus-specific telomeric proteins. Genes Dev 1993;7:883-894.
34. Moore MAS. Does stem cell exhaustion result from combining hematopoietic growth factors with chemotherapy? If so, how do we prevent it? Blood 1992;80:3-7.