-
1
-
-
0029563009
-
-
V. A. Zakian, Science 270, 1601 (1995).
-
(1995)
Science
, vol.270
, pp. 1601
-
-
Zakian, V.A.1
-
2
-
-
0029128798
-
-
J. Lingner, J. P. Cooper, T. R. Cech, ibid. 269, 1533 (1995); J. D. Watson, Nature New Biol. 239, 197 (1972).
-
(1995)
Science
, vol.269
, pp. 1533
-
-
Lingner, J.1
Cooper, J.P.2
Cech, T.R.3
-
3
-
-
0015515155
-
-
J. Lingner, J. P. Cooper, T. R. Cech, ibid. 269, 1533 (1995); J. D. Watson, Nature New Biol. 239, 197 (1972).
-
(1972)
Nature New Biol.
, vol.239
, pp. 197
-
-
Watson, J.D.1
-
4
-
-
0022402513
-
-
C. W. Greider and E. H. Blackburn, Cell 43, 405 (1985); ibid. 51, 887 (1987); M. S. Singer and D. E. Gottschling, Science 266, 404 (1994).
-
(1985)
Cell
, vol.43
, pp. 405
-
-
Greider, C.W.1
Blackburn, E.H.2
-
5
-
-
0023663881
-
-
C. W. Greider and E. H. Blackburn, Cell 43, 405 (1985); ibid. 51, 887 (1987); M. S. Singer and D. E. Gottschling, Science 266, 404 (1994).
-
(1987)
Cell
, vol.51
, pp. 887
-
-
-
6
-
-
0027944347
-
-
C. W. Greider and E. H. Blackburn, Cell 43, 405 (1985); ibid. 51, 887 (1987); M. S. Singer and D. E. Gottschling, Science 266, 404 (1994).
-
(1994)
Science
, vol.266
, pp. 404
-
-
Singer, M.S.1
Gottschling, D.E.2
-
7
-
-
0026563895
-
-
J. H. Wright, D. E. Gottschling, V. A. Zakian, Genes Dev. 6, 197 (1992); A. J. Lustig, S. Kurtz, D. Shore, Science 250, 549 (1990); F. Klein et al., J. Cell Biol. 117, 935 (1992).
-
(1992)
Genes Dev.
, vol.6
, pp. 197
-
-
Wright, J.H.1
Gottschling, D.E.2
Zakian, V.A.3
-
8
-
-
0025252456
-
-
J. H. Wright, D. E. Gottschling, V. A. Zakian, Genes Dev. 6, 197 (1992); A. J. Lustig, S. Kurtz, D. Shore, Science 250, 549 (1990); F. Klein et al., J. Cell Biol. 117, 935 (1992).
-
(1990)
Science
, vol.250
, pp. 549
-
-
Lustig, A.J.1
Kurtz, S.2
Shore, D.3
-
9
-
-
0026693363
-
-
J. H. Wright, D. E. Gottschling, V. A. Zakian, Genes Dev. 6, 197 (1992); A. J. Lustig, S. Kurtz, D. Shore, Science 250, 549 (1990); F. Klein et al., J. Cell Biol. 117, 935 (1992).
-
(1992)
J. Cell Biol.
, vol.117
, pp. 935
-
-
Klein, F.1
-
10
-
-
0025222442
-
-
M. N. Conrad, J. H. Wright, A. J. Wolf, V. A. Zakian, Cell 63, 739 (1990).
-
(1990)
Cell
, vol.63
, pp. 739
-
-
Conrad, M.N.1
Wright, J.H.2
Wolf, A.J.3
Zakian, V.A.4
-
11
-
-
0001934324
-
-
E. Blackburn and C. Greider, Eds. Cold Spring Harbor Press, Plainview, NY
-
C. B. Harley, in Telomeres, E. Blackburn and C. Greider, Eds. (Cold Spring Harbor Press, Plainview, NY, 1995), pp. 247-263.
-
(1995)
Telomeres
, pp. 247-263
-
-
Harley, C.B.1
-
14
-
-
0029102354
-
-
M. J. McEachern and E. H. Blackburn, Nature 376, 403 (1995); A. Krauskopf and E. H. Blackburn, ibid. 383, 354 (1996); S. Buck, thesis, Columbia University (1996).
-
(1995)
Nature
, vol.376
, pp. 403
-
-
McEachern, M.J.1
Blackburn, E.H.2
-
15
-
-
0029743764
-
-
M. J. McEachern and E. H. Blackburn, Nature 376, 403 (1995); A. Krauskopf and E. H. Blackburn, ibid. 383, 354 (1996); S. Buck, thesis, Columbia University (1996).
-
(1996)
Nature
, vol.383
, pp. 354
-
-
Krauskopf, A.1
Blackburn, E.H.2
-
16
-
-
0029102354
-
-
thesis, Columbia University
-
M. J. McEachern and E. H. Blackburn, Nature 376, 403 (1995); A. Krauskopf and E. H. Blackburn, ibid. 383, 354 (1996); S. Buck, thesis, Columbia University (1996).
-
(1996)
-
-
Buck, S.1
-
19
-
-
0025201982
-
-
D. E. Gottschling, O. M. Aparicio, B. L. Billington, V. A. Zakian, Cell 63, 751 (1990).
-
(1990)
Cell
, vol.63
, pp. 751
-
-
Gottschling, D.E.1
Aparicio, O.M.2
Billington, B.L.3
Zakian, V.A.4
-
20
-
-
1842360481
-
-
note
-
Plasmid pADE2 (11) was cut with Bam HI and religated, deleting the ADE2 gene. The resulting plasmid (sp59) was cut with Bam HI, end-filled with Klenow polymerase, and ligated with Eco RI-Xba I end-filled fragment from D1724 (23), creating plasmids sp 194 (with the two telomeric sequences in opposite orientation) and sp195 (with the two telomeric sequences in the same orientation). Plasmid sp193 (Tel270 in opposite orientation) was created by inserting an Eco RI-Hind III end-filled fragment from pLTel (73) into sp59 cut with Bam HI. Yeast strains used in this study are all derivatives of Lev95 (MATa ade2-1 trp1-1 leu2-3, 112his3-11, 15 URA3-1 can1-100 rap1::LEU2 pRAP1-SUP4-0), which is itself derived from strain W303-1B. Lev143/144, Lev165/ 166, and Lev153/154/155 result from the transformation of Lev95 with the linearized plasmids sp194, sp195, and sp193, respectively. Lev7 and Lev8 are described in (24).
-
-
-
-
21
-
-
0024306914
-
-
M. S. Longtine, N. M. Wilson, M. E. Petracek, J. Berman, Curr. Genet. 16, 225 (1989); E. Gilson, M. Roberge, R. Giraldo, D. Rhodes, S. M. Gasser, J. Mol. Biol. 231, 293 (1993); E. Gilson et al., Nucleic Acids Res. 22, 5310 (1994).
-
(1989)
Curr. Genet.
, vol.16
, pp. 225
-
-
Longtine, M.S.1
Wilson, N.M.2
Petracek, M.E.3
Berman, J.4
-
22
-
-
0027298713
-
-
M. S. Longtine, N. M. Wilson, M. E. Petracek, J. Berman, Curr. Genet. 16, 225 (1989); E. Gilson, M. Roberge, R. Giraldo, D. Rhodes, S. M. Gasser, J. Mol. Biol. 231, 293 (1993); E. Gilson et al., Nucleic Acids Res. 22, 5310 (1994).
-
(1993)
J. Mol. Biol.
, vol.231
, pp. 293
-
-
Gilson, E.1
Roberge, M.2
Giraldo, R.3
Rhodes, D.4
Gasser, S.M.5
-
23
-
-
0028631862
-
-
M. S. Longtine, N. M. Wilson, M. E. Petracek, J. Berman, Curr. Genet. 16, 225 (1989); E. Gilson, M. Roberge, R. Giraldo, D. Rhodes, S. M. Gasser, J. Mol. Biol. 231, 293 (1993); E. Gilson et al., Nucleic Acids Res. 22, 5310 (1994).
-
(1994)
Nucleic Acids Res.
, vol.22
, pp. 5310
-
-
Gilson, E.1
-
24
-
-
1842394315
-
-
note
-
G site was ligated with BgI II-cut sp180 to give plasmid sp181. Lev130, Lev132, and Lev134 result from the transformation of Lev95 with the linearized plasmids sp179, sp180, and sp181, respectively.
-
-
-
-
28
-
-
1842265026
-
-
note
-
In both wild-type and mutant cells, we observed a nonlinear response with increasing numbers of Gbd/ Rap1 hybrids targeted to the telomere. One possible explanation is that full site occupancy may not have been achieved in these experiments, particularly with larger numbers of sites (three or four).
-
-
-
-
29
-
-
0028004378
-
-
P. Moretti, K. Freeman, L. Coodley, D. Shore, Genes Dev. 8, 2257 (1994).
-
(1994)
Genes Dev.
, vol.8
, pp. 2257
-
-
Moretti, P.1
Freeman, K.2
Coodley, L.3
Shore, D.4
-
30
-
-
0027326367
-
-
G. Kyrion, K. Liu, C. Liu, A. J. Lustig, ibid. 7, 1146 (1993); M. Cockell et al., J. Cell Biol. 129, 909 (1995); A. Hecht, S. Strahl-Bolsinger, M. Grunstein, Nature 383, 92 (1996); C. Liu and A. J. Lustig, Genetics 143, 81 (1996).
-
(1993)
Genes Dev.
, vol.7
, pp. 1146
-
-
Kyrion, G.1
Liu, K.2
Liu, C.3
Lustig, A.J.4
-
31
-
-
0029048189
-
-
G. Kyrion, K. Liu, C. Liu, A. J. Lustig, ibid. 7, 1146 (1993); M. Cockell et al., J. Cell Biol. 129, 909 (1995); A. Hecht, S. Strahl-Bolsinger, M. Grunstein, Nature 383, 92 (1996); C. Liu and A. J. Lustig, Genetics 143, 81 (1996).
-
(1995)
J. Cell Biol.
, vol.129
, pp. 909
-
-
Cockell, M.1
-
32
-
-
0029817763
-
-
G. Kyrion, K. Liu, C. Liu, A. J. Lustig, ibid. 7, 1146 (1993); M. Cockell et al., J. Cell Biol. 129, 909 (1995); A. Hecht, S. Strahl-Bolsinger, M. Grunstein, Nature 383, 92 (1996); C. Liu and A. J. Lustig, Genetics 143, 81 (1996).
-
(1996)
Nature
, vol.383
, pp. 92
-
-
Hecht, A.1
Strahl-Bolsinger, S.2
Grunstein, M.3
-
33
-
-
0030009208
-
-
G. Kyrion, K. Liu, C. Liu, A. J. Lustig, ibid. 7, 1146 (1993); M. Cockell et al., J. Cell Biol. 129, 909 (1995); A. Hecht, S. Strahl-Bolsinger, M. Grunstein, Nature 383, 92 (1996); C. Liu and A. J. Lustig, Genetics 143, 81 (1996).
-
(1996)
Genetics
, vol.143
, pp. 81
-
-
Liu, C.1
Lustig, A.J.2
-
34
-
-
0028941966
-
-
S. W. Buck and D. Shore, Genes Dev. 9, 370 (1995); C. Boscheron et al., EMBO J. 15, 2184 (1996).
-
(1995)
Genes Dev.
, vol.9
, pp. 370
-
-
Buck, S.W.1
Shore, D.2
-
35
-
-
0029879358
-
-
S. W. Buck and D. Shore, Genes Dev. 9, 370 (1995); C. Boscheron et al., EMBO J. 15, 2184 (1996).
-
(1996)
EMBO J.
, vol.15
, pp. 2184
-
-
Boscheron, C.1
-
36
-
-
0027423154
-
-
F. Palladino et al., Cell 75, 543 (1993).
-
(1993)
Cell
, vol.75
, pp. 543
-
-
Palladino, F.1
-
37
-
-
84907166550
-
-
C.-T. Chien, S. Buck, R. Sternglanz, D. Shore, ibid., p. 531.
-
Cell
, pp. 531
-
-
Chien, C.-T.1
Buck, S.2
Sternglanz, R.3
Shore, D.4
-
38
-
-
0029741576
-
-
S. Marcand, S. W. Buck, P. Moretti, E. Gilson, D. Shore, Genes Dev. 10, 1297 (1996).
-
(1996)
Genes Dev.
, vol.10
, pp. 1297
-
-
Marcand, S.1
Buck, S.W.2
Moretti, P.3
Gilson, E.4
Shore, D.5
-
39
-
-
0029845892
-
-
C. I. Nugent, T. R. Hughes, N. F. Lue, V. Lundblad, Science 274, 249 (1996); J.-J. Lin and V. A. Zakian, Proc. Natl. Acad. Sci. U.S.A. 93, 13760 (1996); N. Grandin, S. I. Reed, M. Charbonneau, Genes Dev., in press.
-
(1996)
Science
, vol.274
, pp. 249
-
-
Nugent, C.I.1
Hughes, T.R.2
Lue, N.F.3
Lundblad, V.4
-
40
-
-
0030447657
-
-
C. I. Nugent, T. R. Hughes, N. F. Lue, V. Lundblad, Science 274, 249 (1996); J.-J. Lin and V. A. Zakian, Proc. Natl. Acad. Sci. U.S.A. 93, 13760 (1996); N. Grandin, S. I. Reed, M. Charbonneau, Genes Dev., in press.
-
(1996)
Proc. Natl. Acad. Sci. U.S.A.
, vol.93
, pp. 13760
-
-
Lin, J.-J.1
Zakian, V.A.2
-
41
-
-
0029845892
-
-
in press
-
C. I. Nugent, T. R. Hughes, N. F. Lue, V. Lundblad, Science 274, 249 (1996); J.-J. Lin and V. A. Zakian, Proc. Natl. Acad. Sci. U.S.A. 93, 13760 (1996); N. Grandin, S. I. Reed, M. Charbonneau, Genes Dev., in press.
-
Genes Dev.
-
-
Grandin, N.1
Reed, S.I.2
Charbonneau, M.3
-
42
-
-
1842308468
-
-
note
-
G sites is still insufficient to reduce telomere length (17); this indicates that a threshold number of Rap1 p COOH-terminal domains, not obtained under these conditions, is required to effectively inhibit telomere elongation.
-
-
-
-
43
-
-
0026623241
-
-
C. F. J. Hardy, L. Sussel, D. Shore, Genes Dev. 6, 801 (1992); D. Wotton and D. Shore, ibid., in press.
-
(1992)
Genes Dev.
, vol.6
, pp. 801
-
-
Hardy, C.F.J.1
Sussel, L.2
Shore, D.3
-
44
-
-
0026623241
-
-
in press
-
C. F. J. Hardy, L. Sussel, D. Shore, Genes Dev. 6, 801 (1992); D. Wotton and D. Shore, ibid., in press.
-
Genes Dev.
-
-
Wotton, D.1
Shore, D.2
-
47
-
-
0029586089
-
-
L. Chong et al., Science 270, 1663 (1995); T. Bilaud et al., Nucleic Acids Res. 24, 1294 (1996).
-
(1995)
Science
, vol.270
, pp. 1663
-
-
Chong, L.1
-
48
-
-
0029879223
-
-
L. Chong et al., Science 270, 1663 (1995); T. Bilaud et al., Nucleic Acids Res. 24, 1294 (1996).
-
(1996)
Nucleic Acids Res.
, vol.24
, pp. 1294
-
-
Bilaud, T.1
-
50
-
-
1842271896
-
-
note
-
We thank S. Buck, P. Moretti, D. Gottschling, and R. Rothstein for plasmids and strains; S. Buck for helpful discussions and technical advice; and R. Rothstein, D. Wotton, V. Alvaro, and members of the Gilson lab for comments. Supported by NIH grant GM40094 (to D.S.) and grants from Association pour la Recherche contre le Cancer (ARC), Association Française de Lutte contre la Mucovisidose (AFLM), Ligue Nationale contre le Cancer, and Région Rhônes-Alpes (to E.G.).
-
-
-
|