Two novel gene expression systems based on the yeasts Schwanniomyces occidentalis and Pichia stipitis

Applied Microbiology and Biotechnology

Volumn 50, Issue 3, 1998, Pages 331-338

  Piontek, M ^a   Hagedorn, J ^a   Hollenberg, C P ^b   Gellissen, G ^a   Strasser, A W M ^a  

^a Rhein Biotech GmbH   (Germany)

^b HEINRICH HEINE UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; ENDOMYCETALES; FUNGAL GENETICS; GENE EXPRESSION; NONHUMAN; PROMOTER REGION; SPECIES DIFFERENCE; YEAST;

CELLULASE; CLOSTRIDIUM; GENE EXPRESSION; GENES, BACTERIAL; GENES, REPORTER; GENETIC VECTORS; PHYSICAL CHROMOSOME MAPPING; PICHIA; PLASMIDS; PROMOTER REGIONS (GENETICS); RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCETALES; TERMINATOR REGIONS (GENETICS); TRANSFORMATION, GENETIC;

BACTERIA (MICROORGANISMS); CLOSTRIDIUM THERMOCELLUM; DEBARYOMYCES OCCIDENTALIS; FUNGI; MYXOGASTRIA; PICHIA STIPITIS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCETALES;

EID: 0031692690     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002530051300     Document Type: Article

References (47)

1. Amore R, Kötter P, Küster C, Ciriacy M, Hollenberg CP (1991) Cloning and expression in Saccharomyces cerevisiae of the NAD(P)H-dependent xylose reductase encoding gene (XYL1) from the xylose-assimilating yeast Pichia stipitis. Gene 109: 89-97
2. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (eds) (1987) Current protocols in molecular biology. J Wiley, New York
3. Barnett JA (1976) The utilization of sugars by yeast. In: Tipson RS, Horton D (eds) Advances in carbohydrate chemistry and biochemistry. Academic Press, New York, pp 125-235
4. Béguin P (1983) Detection of cellulase activity in polyacrylamide gels using Congo red-stained agar replicas. Anal Biochem 131: 333-336
5. Berg JA van den, Laken KJ van der, Ooyen AJJ van, Renniers TCHM, Rietveld D, Schaap A, Brake AJ, Bishop RJ, Schultz K, Moyer D, Richman M, Shuster JR (1990) Kluyveromyces as a host for heterologous gene expression: expression and secretion of prochymosin. Biotechnology 8: 15-139
6. Birnhoim HC, Doly J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7: 1513-1521
7. Bolivar F, Rodriguez RL, Greene PJ, Betlach MC, Heynecker HL, Boyer HW, Crosa JH, Falkow S (1977) Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene 2: 95-113
8. Boyer HW, Roulland-Dussoix D (1969) A complementation analysis of the restriction and modification of DNA in Escherichia coli. J Mol Biol 41: 459-472
9. Boze H, Guyot JB, Moulin G, Galzy P (1989) Kinetics of the amyloglucosidase of Schwanniomyces castellii. In: Martini A, Vaughan-Martini A (eds) Yeast as a main protagonist of biotechnology. Yeast 2: 117-121
10. Cornet P, Millet J, Béguin P, Aubert J-P (1983) Characterization of two cel (cellulose degradation) genes of Clostridium thermocellum coding for endoglucanase. Biotechnology 1: 589-594
11. Dohmen RJ (1989) Klonierung des Glukoamylase-Gens der Hefe Schwanniomyces occidentalis und seine Expression in der Bäckerhefe Saccharomyces cerevisiae. PhD thesis, Heinrich-Heine Universität Düsseldorf
12. Dohmen RJ, Strasser AWM, Zitomer RS, Hollenberg CP (1989) Regulated overproduction of α-amylase by transformation of the amylolytic yeast Schwanniomyces cerevisiae. Curr Genet 15: 319-325
13. Dohmen RJ, Strasser AWM, Dahlems UM, Hollenberg CP (1990) Cloning of the Schwanniomyces occidentalis glucoamylase gene (GAM1) and its expression in Saccharomyces cerevisiae. Gene 95: 111-121
14. Dohmen RJ, Strasser AWM, Höner CB, Hollenberg CP (1991) An efficient transformation procedure enabling long-term storage of competent cells of various yeast genera. Yeast 7: 691-692
15. Gellissen G, Hollenberg CP (1997) Application of yeasts in gene expression studies: a comparison of Saccharomyces cerevisiae. Hansenula polymorpha and Kluyveromyces lactis - a review. Gene 190: 87-97
16. Gellissen G, Melber K (1996) Methylotrophic yeast Hansenula polymorpha as production organism for recombinant pharmaceuticals. Drug Res 46: 943-948
17. Gellissen G, Janowicz ZA, Merckelbach A, Piontek M, Keup P, Weydemann U, Hollenberg CP, Strasser AWM (1991) Heterologous gene expression in Hansenula polymorphic, efficient secretion of glucoamylase. Biotechnology 9: 291-295
18. Gibson TJ (1984) Studies on the Epstein-Barr virus genome. PhD thesis, Cambridge University
19. Hagedorn J (1990) Isolierung und Charakterisierung von Mutanten im Xylosestoffwechsel und Entwicklung eines Transformationssystems für die Hefe Pichia stipitis. PhD thesis, Heinrich-Heine-Universität Düsseldorf
20. Hanahan D (1983) Studies on transformation of Escherichia coli with plasmids. J Mol Biol 66: 557-580
21. Hansen H, Hollenberg CP (1996) Hansenula polymorpha (Pichia angusta). In: Wolf K (ed) Nonconventional yeasts in biotechnology. Springer, Heidelberg Berlin New York, pp 293-311
22. Heukeshoven J, Dernick R (1988) Improved silver staining procedure for fast staining in PhastSystem Development Unit I. Staining of sodium dodecyl sulfate gels. Electrophoresis 9: 28-32
23. Hinnen A, Buxton E, Chaudhuri B, Heim J, Hottiger T, Mayhack B, Pohlig G (1994) Gene expression in recombinant yeast. In: Smith A (ed) Gene expression in recombinant microorganisms. Dekker, New York, pp 121-193
24. Hitzeman RA, Hagie FE, Levine HL, Goeddel DV, Ammerer G, Hall BD (1981) Expression of a human gene for interferon in yeast. Nature 293: 717-722
25. Hollenberg CP, Gellissen G (1997) Production of recombinant proteins by methylotrophic yeasts. Curr Opin Biotechnol 8: 554-560
26. Joliff G, Béguin P, Aubert J-P (1986a) Nucleotide sequence of the cellulase gene cell) encoding endoglucanase D of Clostridium thermocellum. Nucleic Acids Res 14: 8605-8613
27. Joliff G, Béguin P, Juy M, Millet J, Ryter A, Poljak R, Aubert J-P (1986b) Isolation, crystallization and properties of a new cellulase of Clostridium thermocellum overproduced in E. coli. Biotechnology 4: 896-900
28. Kellermann E, Seeboth P, Hollenberg CP (1986) Analysis and function of the primary structure and promoter function of a pyruvate decarboxylase gene (PDC1) from Saccharomyces cerevisiae. Nucleic Acids Res 14: 8963-8977
29. Klein RD, Favreau MA (1988) Transformation of Schwanniomyces occidentalis with an ADE2 gene cloned from S. occidentalis. J Bacteriol 170: 5572-5578
30. Klein RD, Favreau MA (1991) A DNA fragment containing the ADE2 gene from Schwanniomyces occidentalis can be maintained as an extrachromosomal element. Gene 97: 183-189
31. Kötter P, Amore R, Hollenberg CP, Ciriacy M (1990) Isolation and characterization of the Pichia stipitis xylitol dehydrogenase gene. XYL2, and construction of a xylose-utilizing Saccharomyces cerevisiae mutant. Curr Genet 18: 493-500
32. Laemmli UK (1970) Cleavage of structural proteins during assembly of the head of the bacteriophage T4. Nature 227: 680-685
33. Lodder J (1984) The yeasts: a taxonomic study. North Holland, Amsterdam
34. Ma H, Kunes S, Schatz PJ, Botstein D (1987) Plasmid construction by homologous recombination in yeast. Gene 58: 201-216
35. Mellor J, Dobson MJ, Roberts NA, Kingsman AJ, Kingsman SM (1985) Factors affecting heterologous gene expression in Saccharomyces cerevisiae. Gene 33: 215-226
36. Millet J, Pétré D, Béguin P, Raynaud O, Aubert J-P (1985) Cloning of ten distinct DNA fragments of Clostridium thermocellum coding for cellulase. FEMS Microbiol Lett 29: 145-149
37. Nelson N (1944) A photometric adaptation of the Somogyi method for the determination of glucose. J Biol Chem 153: 275-380
38. Pichuantes S, Nguyen AT, Franzusoff A (1996) Expression of heterologous gene products in yeast. In: Cleland JL, Craik CS (eds) Protein engineering: principles and practice. Wiley Liss, New York, pp 129-161
39. Rocha TL, Paterson G, Crimmins K, Boyd A, Sawyer L, Fothergill-Gilmore LA (1996) Expression and secretion of recombinant ovine β-lactoglobulin in Saccharomyces cerevisiae and Kluyveromyces lactis. Biochem J 313: 927-932
40. Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain termination inhibitors. Proc Natl Acad Sci USA 74: 5463-5467
41. Sreekrishna K, Kropp KE (1996) Pichia pastoris. In: Wolf K (ed) Nonconventional yeasts in biotechnology. Springer, Berlin, pp 203-253
42. Strasser AWM, Selk R, Dohmen RJ, Niermann T, Bielefeld M, Seeboth P, Tu G, Hollenberg CP (1989) Analysis of the α-amylase gene of Schwanniomyces occidentalis and the secretion of ist gene product in transformants of different yeast genera. Eur J Biochem 184: 699-706
43. Struhl K (1987) Promoters, activator proteins, and the mechanism of transcriptional initiation in yeast. Cell 49: 295-297
44. Teather RM, Wood PJ (1982) Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulytic bacteria from the bovine rumen. Appl Environ Microbiol 43: 777-780
45. Wésolowski-Louvel M, Breunig KD, Fukuhara H (1996) Kluyveromyces lactis. In: Wolf K (ed) Nonconventional yeasts in biotechnology. Springer, Berlin, pp 139-201
46. Wickens M, Stephenson B (1984) Role of conserved AAUAAA sequence: four AAUAAA point mutations prevent messenger 3′ end formation. Science 226: 1045-1051
47. Wolf K (ed) (1996) Nonconventional yeasts in biotechnology. Springer, Berlin