Tag games in yeast: The two-hybrid system and beyond

Current Opinion in Biotechnology

Volumn 8, Issue 5, 1997, Pages 561-568

  Brachmann, Rainer K ^a   Boeke, Jef D ^b  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

LIGAND; PEPTIDE; PROTEIN; PROTEIN KINASE; RNA;

BIOTECHNOLOGY; DNA REPAIR; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; PROTEIN RNA BINDING; REVIEW; SACCHAROMYCES CEREVISIAE; YEAST;

SACCHAROMYCES CEREVISIAE;

EID: 0030690331     PISSN: 09581669     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0958-1669(97)80029-8     Document Type: Article

References (55)

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31. •].
32. ••].
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40. ••] can be successfully used to isolate a novel RNA-binding protein for the histone RNA 3′ hairpin.
41. ••] can be successfully used to isolate a novel RNA-binding protein for the histone RNA 3′ hairpin.
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46. ••] can be put to successful use. The authors show that by using both HIS3 and SPO13-URA3 reporters, point mutations that identify protein regions required for binding of E2F-1 to DP1 can be identified.
47. ••] can be put to successful use. It provides an example of macromolecular dissociators by identifying and characterizing dominant-negative p53 mutants.
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50. ••] can be put to successful use. The system can also be used to identify dissociators in library screens, as was shown by isolating a known p53 dissociator, the MDM2 protein, in such a screen.
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