Preparative two-dimensional gel electrophoresis with agarose gels in the first dimension for high molecular mass proteins

Electrophoresis

Volumn 21, Issue 9, 2000, Pages 1653-1669

  Oh Ishi, Masamichi ^a   Satoh, Mamoru ^a   Maeda, Tadakazu ^a  

^a KITASATO UNIVERSITY   (Japan)

Author keywords

Agarose gel; High molecular mass proteins; Two dimensional gel electrophoresis

Indexed keywords

ELECTROPHORESIS; MAMMALS; MOLECULAR MASS; TRICHLOROACETIC ACID; UREA;

AGAROSE; AGAROSE GEL; AGAROSE ISOELECTRIC FOCUSING; ELECTROPHORETIC SEPARATIONS; HIGH MOLECULAR MASS; HIGH MOLECULAR MASS PROTEIN; LOADING CAPACITIES; PH GRADIENTS; TWO-DIMENSIONAL GEL ELECTROPHORESIS; TWO-DIMENSIONAL GEL ELECTROPHORESIS (2-DE);

PROTEINS;

3 [(3 CHOLAMIDOPROPYL)DIMETHYLAMMONIO] 1 PROPANESULFONIC ACID; AGAROSE; MERCAPTOETHANOL; PROTEINASE INHIBITOR; THIOUREA; TRICHLOROACETIC ACID; UREA;

ANALYTIC METHOD; ANIMAL TISSUE; CONFERENCE PAPER; INTERMETHOD COMPARISON; ISOELECTRIC FOCUSING; MOLECULAR WEIGHT; NONHUMAN; PROTEIN ANALYSIS; TWO DIMENSIONAL GEL ELECTROPHORESIS;

EID: 0034090053     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1522-2683(20000501)21:9<1653::AID-ELPS1653>3.0.CO;2-9     Document Type: Conference Paper

References (18)

1. Hirabayashi, T., Anal. Biochem. 1981, 117, 443-451.
2. Oh-Ishi, M., Hirabayashi, T., Comp. Biochem. Physiol. B 1989, 92, 609-617.
3. Hirabayashi, T., Electrophoresis 2000, 21, 446-451.
4. O'Farrell, P. H., J. Biol. Chem. 1975, 250, 4007-4021.
5. Piperno, G., Huang, B., Ramanis, Z., Luck, D. J. L., J. Cell Biol. 1981, 88, 73-79.
6. Celis, J. E., Gesser, B., Rasmussen, H. H., Madsen, P., Leffers, H., Dejgaard, K., Honoré, B., Olsen, E., Ratz, G., Lauridsen, J. B., Basse, B., Mouritzen, S., Hellerup, M., Andersen, A., Walbum, E., Celis, A., Bauw, G., Puype, M., Van Damme, J., Vandekerckhove, J., Electrophoresis 1990, 11, 989-1071.
7. Hori, S., Sugiura, H., Shimizu, T., Hirabayashi, T., Ohtani, S., Yoshida, M., Miyamoto, K., Tanabe, H., Biochem. Biophys. Res. Commun. 1989, 161, 726-731.
8. Bjellqvist, B., Ek, K., Righetti, P. G., Gianazza, E., Görg, A., Westermeier, R., Postel, W., J. Biochem. Biophys. Methods 1982, 6, 317-339.
9. Bradford, M. M., Anal. Biochem. 1976, 72, 248-254.
10. Oh-Ishi, M., Hirabayashi, T., Physico-Chem. Biol. 1988, 32, 113-120.
11. Görg, A., Postel, W., Günther, S., Electrophoresis 1988, 9, 531-546.
12. Laemmli, U. K., Nature 1970, 227, 680-685.
13. Nakamura, M., Imai, H., Hirabayashi, T., Dev. Biol. 1989, 132, 389-397.
14. Rasband, W., 1995, the latest version of NIH Image is available to Internet users via anonymous FTP from zippy.ninh.nih.gov.
15. Giometti, C. S., CRC Rev. Clin. Sci. 1982, 18, 79-109.
16. Oh-Ishi, M., Omori, A., Kwon, J.-Y., Agui, T., Maeda, T., Furudate, S.-I., Endocrinology 1998, 139, 1288-1299.
17. Rabilloud, T., Adessi, C., Giraudel, A., Lunardi, J., Electrophoresis 1997, 18, 307-316.
18. Yates, L. D., Greaser, M. L., J. Mol. Biol. 1983, 168, 123-141.