Simplification of complex peptide mixtures for proteomic analysis: Reversible biotinylation of cysteinyl peptides

Electrophoresis

Volumn 21, Issue 9, 2000, Pages 1635-1650

  Spahr, Chris S ^a   Susin, Santos A ^b   Bures, Edward J ^a   Robinson, John H ^a   Davis, Michael T ^a   McGinley, Michael D ^a   Kroemer, Guido ^b   Patterson, Scott D ^a  

^a AMGEN INC   (United States)

^b CNRS   (France)

Author keywords

Affinity chromatography; Apoptosis; Cysteine biotinylation; Mass spectrometer; Mitochondria

Indexed keywords

AMINO ACIDS; CELL DEATH; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IONS; MASS SPECTROMETERS; MASS SPECTROMETRY;

BIOTINYLATIONS; COMPLEX PEPTIDE MIXTURES; CYSTEINE BIOTINYLATION; ENZYMATIC DIGESTIONS; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY; ION SELECTION; MITOCHONDRIAS; PROTEIN FRACTIONS; PROTEOMIC ANALYSIS; REVERSED-PHASE HIGH PERFORMANCE;

PEPTIDES;

CYSTEINE; PEPTIDE; PROTEOME;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; APOPTOSIS; BIOTINYLATION; CONFERENCE PAPER; MASS SPECTROMETRY; MITOCHONDRION; PROTEIN ANALYSIS; PROTEIN PURIFICATION; PROTEIN SECRETION; UNINDEXED SEQUENCE;

EID: 0034121916     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1522-2683(20000501)21:9<1635::AID-ELPS1635>3.0.CO;2-1     Document Type: Conference Paper

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