Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis

Electrophoresis

Volumn 19, Issue 5, 1998, Pages 837-844

  Molloy, Mark P ^a   Herbert, Ben R ^a,b   Walsh, Bradley J ^a   Tyler, Margaret I ^a   Traini, Mathew ^a   Sanchez, Jean Charles ^c   Hochstrasser, Denis F ^c   Williams, Keith L ^a   Gooley, Andrew A ^a  

^a MACQUARIE UNIVERSITY   (Australia)

^b Wool Res Organisation New Zealand   (New Zealand)

^c GENEVA UNIVERSITY HOSPITALS   (Switzerland)

Author keywords

Escherichia coli; Membrane proteins; Proteome; Solubility; Two dimensional polyacrylamide gel electrophoresis

Indexed keywords

ELECTROPHORESIS; ESCHERICHIA COLI; EXTRACTION; MEMBRANES; METABOLISM; PHOSPHORUS COMPOUNDS; SOLUBILITY; UREA;

CELL LYSATES; E. COLI; ESCHERICHIA COLI CELLS; EXTRACTION AND ENRICHMENTS; MEMBRANE PROTEINS; PREFRACTIONATION; SIMPLE++; SOLUBILISATION; TWO-DIMENSIONAL GEL ELECTROPHORESIS; TWO-DIMENSIONAL POLYACRYLAMIDE GEL ELECTROPHORESIS;

PROTEINS;

BACTERIAL PROTEIN; MEMBRANE PROTEIN; OUTER MEMBRANE PROTEIN;

CELL LYSATE; CONFERENCE PAPER; ESCHERICHIA COLI; EXTRACTION; ISOELECTRIC FOCUSING; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; RECEPTOR SOLUBILIZATION; SOLUBILITY; SOLUBILIZATION; TWO DIMENSIONAL ELECTROPHORESIS;

EID: 0031807348     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/elps.1150190539     Document Type: Conference Paper

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