Single-cell imaging of intracellular Ca2+ and phospholipase C activity reveals that RGS 2, 3, and 4 differentially regulate signaling via the Gαq/11-linked muscarinic M3 receptor

Molecular Pharmacology

Volumn 66, Issue 6, 2004, Pages 1453-1464

  Tovey, Stephen C ^a   Willars, Gary B ^a  

^a UNIVERSITY OF LEICESTER   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM ION; DIACYLGLYCEROL; G ALPHA 11 COUPLED MUSCARINIC M3 RECEPTOR; G ALPHA Q COUPLED MUSCARINIC M3 RECEPTOR; G PROTEIN COUPLED RECEPTOR; INOSITOL 1,4,5 TRISPHOSPHATE; INOSITOL PHOSPHATE; MUSCARINIC M3 RECEPTOR; MYC PROTEIN; PHOSPHATIDYLINOSITIDE; PHOSPHOLIPASE C; PROTEIN; RGS 3 PROTEIN; RGS PROTEIN; RGS2 PROTEIN; RGS4 PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BIOSENSOR; CALCIUM CELL LEVEL; CELL POPULATION; CELLULAR DISTRIBUTION; CONFOCAL MICROSCOPY; CONTROLLED STUDY; DIAGNOSTIC IMAGING; EMBRYO; ENZYME ACTIVITY; HUMAN; HUMAN CELL; NUCLEOTIDE SEQUENCE; OSCILLATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; SIGNAL TRANSDUCTION;

CALCIUM; CELL LINE; GENES, MYC; GTP-BINDING PROTEIN ALPHA SUBUNITS, GQ-G11; GTP-BINDING PROTEINS; GTPASE-ACTIVATING PROTEINS; HOMEOSTASIS; HUMANS; INOSITOL 1,4,5-TRISPHOSPHATE; KIDNEY; METHACHOLINE CHLORIDE; MICROSCOPY, CONFOCAL; PHOSPHOLIPASE C; RECEPTOR, MUSCARINIC M3; RECOMBINANT PROTEINS; RGS PROTEINS; SIGNAL TRANSDUCTION; TRANSFECTION;

EID: 9444274906     PISSN: 0026895X     EISSN: None     Source Type: Journal    
DOI: 10.1124/mol.104.005827     Document Type: Article

References (38)

1. Ancellin N, Preisser L, Le Maout S, Barbado M, Créminon C, Corman B, and Morel A (1999) Homologous and heterologous phosphorylation of the vasopressin V1a receptor. Cell Signal 11:743-751.
2. q-mediated signaling in vivo. J Biol Chem 279:3906-3915.
3. q/11α signaling. J Biol Chem 279:21248-21256.
4. Castro-Fernandez C and Conn PM (2002) Regulation of the gonadotropin-releasing hormone receptor (GnRHR) by RGS proteins: role of the GnRHR carboxyl-terminus. Mol Cell Endocrinol 191:149-156.
5. De Vries L, Zheng B, Fischer T, Elenko E, and Farquhar MG (2000) The regulator of G protein signaling family. Annu Rev Pharmacol Toxicol 40:235-271.
6. + channels. Proc Natl Acad Sci (USA) 94:10461-10466.
7. Hepler JR (1999) Emerging roles for RGS proteins in cell signalling. Trends Pharmacol Sec 20:376-382.
8. Heximer SP, Watson N, Linder ME, Blumer KJ, and Hepler JR (1997) RGS2/G0S8 is a selective inhibitor of Gqα function. Proc Natl Acad Sci (USA) 94:14389-14393.
9. Heximer SP, Srinivasa SP, Bernstein LS, Bernard JL, Linder ME, Hepler JR, and Blumer KJ (1999) G protein selectivity is a determinant of RGS2 function. J Biol Chem 274:34253-34259.
10. Heximer SP, Lim H, Bernard JL, and Blumer KJ (2001) Mechanisms governing subcellular localization and function of human RGS2. J Biol Chem 276:14195-14203.
11. Hollinger S and Hepler JR (2002) Cellular regulation of RGS proteins: modulators and integrators of G protein signaling. Pharmacol Rev 54:527-559.
12. i oscillations. Mol Cell 7:651-660.
13. Mitchell FM, Mullaney I, Godfrey PP, Arkinstall SJ, Wakelam MJO, and Milligan G (1991) Widespread distribution of Gqα/G11α detected immunologically by an antipeptide antiserum directed against the predicted C-terminal decapeptide. FEBS Lett 287:171-174.
14. Nahorski SR, Young KW, Challiss RAJ, and Nash MS (2003) Visualizing phosphoinositide signalling in single neurons gets a green light. Trends Neurosci 26:444-452.
15. 3 production following G-protein-coupled-receptor activation. Biochem J 358:137-142.
16. 2+ and inositol 1,4,5-trisphosphate oscillation frequency: receptor density versus agonist concentration. J Biol Chem 277:35947-35960.
17. Neill JD, Duck LW, Sellers JC, Musgrove LC, Scheschonka A, Druey KM, and Kehrl JH (1997) Potential role for a regulator of G protein signaling (RGS3) in gonadotropin-releasing hormone (GnRH) stimulated desensitization. Endocrinol 138:843-846.
18. Oancea E and Meyer T (1998) Protein kinase C as a molecular machine for decoding calcium and diacylglycerol signals. Cell 95:307-318.
19. Oancea E, Teruel MN, Quest AF, and Meyer TJ (1998) Green fluorescent protein (GFP)-tagged cysteine-rich domains from protein kinase C as fluorescent indicators for diacylglycerol signaling in living cells. J Cell Biol 140:485-498.
20. Ross EM and Wilkie TM (2000) GTPase-activating proteins for heterotrimeric G proteins: Regulators of G protein signaling (RGS) and RGS-like proteins. Annu Rev Biochem 69:795-827.
21. Roy AA, Lemberg KE, and Chidiac P (2003) Recruitment of RGS2 and RGS4 to the plasma membrane by G proteins and receptors reflects functional interactions. Mol Pharmacol 64:587-593.
22. q-type G proteins: regulators of G proteins as tools to dissect pertussis toxin-resistant G proteins in receptor-effector coupling. J Biol Chem 276:2474-2479.
23. 11α. Mol Pharmacol 58:719-728.
24. 5 subunits. Proc Natl Acad Sci (USA) 95:13307-13312.
25. Snow BE, Hall RA, Krumins AM, Brothers GM, Bouchard D, Brothers CA, Chung S, Mangion J, Gilman AG, Lefkowitz RJ, et al. (1998b) GTPase activating specificity of RGS12 and binding specificity of an alternatively spliced PDZ (PSD-95/DIg/ ZO-1) domain. J Biol Chem 273:17749-17755.
26. Srinivasa SP, Watson N, Overton MC, and Blumer KJ (1998) Mechanism of RGS4, a GTPase-activating protein for G protein α subunits. J Biol Chem 273:1529-1533.
27. 2 concentration monitored in living cells. Curr Biol 8:343-346.
28. 3-activated elementary calcium signals and are downregulated by prolonged hormonal stimulation to inhibit cellular calcium responses. J Cell Sci 114:3979-3989.
29. 2+ signalling by recombinant human CXCR2 chemokine receptors is potentiated by P2Y nucleotide receptors in HEK cells. Br J Pharmacol 135:1199-1208.
30. Wheldon LM, Nahorski SR, and Willars GB (2001) Inositol 1,4,5-trisphosphate independent calcium signalling by platelet-derived growth factor in the human SH-SY5Y neuroblastoma cell. Cell Calcium 30:95-106.
31. Willars GB, McArdle CA, and Nahorski SR (1998a) Acute desensitization of phospholipase C-coupled muscarinic M3 receptors but not gonadotropin-releasing hormone receptors co-expressed in αT3-1 cells: implications for mechanisms of rapid desensitization. Biochem J 333:301-308.
32. 2+ signalling in human neuroblastoma cells. Br J Pharmacol 114:1133-1142.
33. Willars GB, Nahorski SR, and Challiss RAJ (1998b) Differential regulation of muscarinic acetylcholine receptor-sensitive polyphosphoinositide pools and consequences for signaling in human neuroblastoma cells. J Biol Chem 273:5037-5046.
34. 5 with the regulators of G protein signaling RGS7 and RGS9. Characterization in native tissues and in transfected cells. J Biol Chem 275:24872-24880.
35. q-mediated signaling via a direct protein-protein interaction. J Biol Chem 278:21307-21313.
36. q-coupled receptors. J Biol Chem 274:3549-3556.
37. Zeng W, Xu X, Popov S, Mukhopadhyay S, Chidiac P, Swistokl J, Danho W, Yagaloff KA, Fisher SL, Ross EM, et al. (1998) The N-terminal domain of RGS4 confers receptor-selective inhibition of G protein signaling. J Biol Chem 273:34687-34690.
38. Zheng B, De Vries L, and Gist Farquhar M (1999) Divergence of RGS proteins: evidence for the existence of six mammalian RGS subfamilies. Trends Biochem Sci 24:411-414.