Detection of the M30 Neoepitope as a New Tool to Quantify Liver Apoptosis: Timing and Patterns of Positivity on Frozen and Paraffin-Embedded Sections

American Journal of Clinical Pathology

Volumn 121, Issue 2, 2004, Pages 211-219

  Grassi, Alberto ^a   Susca, Micaela ^a   Ferri, Silvia ^a   Gabusi, Elena ^a   D'Errico, Antonia ^a   Farina, Gabriele ^a   Maccariello, Silvana ^a   Zauli, Daniela ^a   Bianchi, Francesco B ^a   Ballardini, Giorgio ^a,b  

^a UNIVERSITY OF BOLOGNA   (Italy)

^b UNIVERSITY OF BOLOGNA   (Italy)

Author keywords

Apoptosis; Councilman body; Liver disease; M30; Terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate nick end labeling; TUNEL

Indexed keywords

ANTIGEN; CASPASE; CYTOKERATIN 18; DNA FRAGMENT; M30 ANTIGEN; PARAFFIN; UNCLASSIFIED DRUG;

ANTIGEN EXPRESSION; ANTIGEN RETRIEVAL; APOPTOSIS; ARTICLE; CELL GRANULE; CELL INCLUSION; CELL STRUCTURE; CHRONIC HEPATITIS; CLINICAL ARTICLE; CONTROLLED STUDY; CORRELATION ANALYSIS; COUNCILMAN BODY; DIAGNOSTIC VALUE; EMBEDDING; ENZYME MECHANISM; EPITHELIUM CELL; FROZEN SECTION; HUMAN; HUMAN TISSUE; LIVER BIOPSY; LIVER CELL; LIVER TRANSPLANTATION; PRIORITY JOURNAL; PROTEIN DEGRADATION; STAINING;

EID: 9144247682     PISSN: 00029173     EISSN: None     Source Type: Journal    
DOI: 10.1309/UK621LFJ4FX07KDE     Document Type: Article

References (29)

1. Kronenberger B, Ruster B, Lee JH, et al. Hepatocellular proliferation in patients with chronic hepatitis C and persistently normal or abnormal aminotransferase levels. J Hepatol. 2000;33:640-647.
2. Bantel H, Lugering A, Poremba C, et al. Caspase activation correlates with the degree of inflammatory liver injury in chronic hepatitis C virus infection. Hepatology. 2001;34:758-767.
3. Natori S, Rust C, Stadheim LM, et al. Hepatocyte apoptosis is a pathologic feature of human alcoholic hepatitis. J Hepatol. 2001;34:248-253.
4. Ziol M, Tepper M, Lohez M, et al. Clinical and biological relevance of hepatocyte apoptosis in alcoholic hepatitis. J Hepatol. 2001;34:254-260.
5. Patel T, Gores GJ. Apoptosis in liver transplantation: a mechanism contributing to immune modulation, preservation injury, neoplasia, and viral disease. Liver Transpl Surg. 1998;4:42-50.
6. Krams SM, Martinez OM. Apoptosis as a mechanism of tissue injury in liver allograft rejection. Semin Liver Dis. 1998;18:153-167.
7. Nawaz S, Fennell RH. Apoptosis of bile duct epithelial cells in hepatic allograft rejection. Histopathology. 1994;25:137-142.
8. Gao W, Bentley RC, Maiden JF, et al. Apoptosis of sinusoidal endothelial cells is a critical mechanism of preservation injury in rat liver transplantation. Hepatology. 1998;27:1652-1660.
9. Krames SM, Egawa H, Queen MB, et al. Apoptosis as a mechanism of cell death in liver allograft rejection. Transplantation. 1995;59:621-625.
10. Afford SC, Hubscher S, Strain AJ, et al. Apoptosis in the human liver during allograft rejection and end-stage liver disease. J Pathol. 1995;76:373-380.
11. Sedivy R, Gollackner B, Casati B, et al. Apoptotic hepatocytes in rejection and vascular occlusion in live allograft specimens. Histopathology. 1998;32:503-507.
12. Ballardini G, De Raffele E, Groff P, et al. Timing of reinfection and mechanism of hepatocellular damage in transplanted hepatitis C virus-reinfected liver. Liver Transpl. 2002;8:10-20.
13. Charriaut-Marlangue C, Ben-Ari Y. A cautionary note on the use of the TUNEL stain to determine apoptosis. Neuroreport. 1995;7:61-64.
14. Kumar S. Regulation of caspase activation in apoptosis: implications in pathogenesis and treatment of disease. Clin Exp Pharmacol Physiol. 1999;26:295-303.
15. Miura M, Zhu H, Rotello R, et al. Induction of apoptosis in fibroblasts by IL-1b converting enzyme, a mammalian homolog of the C elegans cell death gene ced-3. Cell. 1993;75:653-660.
16. Caulin C, Salvesen SG, Oshima RG. Caspase cleavage of keratin 18 and reorganization of intermediate filaments during epithelial cell apoptosis. J Cell Biol. 1997;138:1379-1394.
17. Yang F, Sun X, Beech W, et al. Antibody to caspase-cleaved actin detects apoptosis in differentiated neuroblastoma and plaque-associated neurons and microglia in Alzheimer's disease. Am J Pathol. 1998;152:379-389.
18. Leers MP, Kolgen W, Bjorklund V, et al. Immunocytochemical detection and mapping of a cytokeratin 18 neo-epitope exposed during early apoptosis. J Pathol. 1999;187:567-572.
19. Austgulen R, Chedwick L, Vogt Isaksen C, et al. Trophoblast apoptosis in human placenta at term as detected by expression of a cytokeratin 18 degradation product of caspase. Arch Pathol Lab Med. 2002;126:1480-1486.
20. Kadyrov M, Kaufmann P, Huppertz B. Expression of a cytokeratin 18 neo-epitope is a specific marker for trophoblast apoptosis in human placenta. Placenta. 2001;22:44-48.
21. Chiu PM, Ngan YS, Khoo US, et al. Apoptotic activity in gestational trophoblastic disease correlates with clinical outcome: assessment by the caspase-related M30 CytoDeath antibody. Histopathology. 2001;38:243-249.
22. Morsi HM, Leers MP, Jager W, et al. The patterns of expression of an apoptosis-related CK18 neoepitope, the bcl-2 proto-oncogene, and the Ki67 proliferation marker in normal, hyperplastic, and malignant endometrium. Int J Gynecol Pathol. 2000;19:118-126.
23. Backus HH, Van Groeningen CJ, Vos W, et al. Differential expression of cell cycle and apoptosis related proteins in colorectal mucosa, primary colon tumours, and liver metastases. J Clin Pathol. 2002;55:206-211.
24. Carr NJ. M30 expression demonstrates apoptotic cells, correlates with in situ end-labeling, and is associated with Ki67 expression in large intestinal neoplasms. Arch Pathol Lab Med. 2000;124:1768-1772.
25. Kusama K, Jiang Y, Ohno J, et al. Immunohistochemical detection of cytokeratin 18 and its neo-epitope in human salivary glands and pleomorphic adenomas. Anticancer Res. 2000;20:2485-2487.
26. Kusama K, Jiang Y, Toguchi M, et al. Use of the monoclonal antibody M30 for detecting HSG cell apoptosis. Anticancer Res. 2000;20:151-154.
27. Ballardini G, Guidi M, Susca M, et al. Bile duct cell apoptosis is a rare event in primary biliary cirrhosis. Dig Liver Dis. 2001;33:151-156.
28. Susca M, Grassi A, Zauli D, et al. Liver inflammatory cells, apoptosis, regeneration and stellate cell activation in non alcoholic steatohepatitis. Dig Liver Dis. 2001;33:768-777.
29. Bantel H, Ruck P, Gregor M, et al. Detection of elevated caspase activation and early apoptosis in liver diseases. Eur J Cell Biol. 2001;80:30-39.