Dynamics of proteolysis and its influence on the accumulation of intracellular recombinant proteins

Enzyme and Microbial Technology

Volumn 27, Issue 10, 2000, Pages 743-748

  Rozkov, A ^a   Schweder, T ^b   Veide, A ^a   Enfors, S O ^a  

^a ROYAL INSTITUTE OF TECHNOLOGY   (Sweden)

^b UNIVERSITY OF GREIFSWALD   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BIODEGRADATION; BIOSYNTHESIS; ENZYME KINETICS; ESCHERICHIA COLI; REACTION KINETICS;

PROTEOLYSIS;

PROTEINS;

BETA GALACTOSIDASE; HYBRID PROTEIN; MUTANT PROTEIN; PROTEIN ZZT2; RECOMBINANT PROTEIN; STAPHYLOCOCCUS PROTEIN A; UNCLASSIFIED DRUG;

BACTERIAL GROWTH; BACTERIUM CULTURE; CHEMICAL REACTION KINETICS; CONFERENCE PAPER; CONTROLLED STUDY; ESCHERICHIA COLI; NONHUMAN; PRODUCTIVITY; PROTEIN DEGRADATION; PROTEIN SYNTHESIS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; STAPHYLOCOCCUS;

EID: 0034548891     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(00)00294-5     Document Type: Conference Paper

References (28)

1. Heterologous gene expression in yeast
2. Partition of the P1 plasmid
3. Stabilization of Escherichia coli tryptophan-production vectors in continuous cultures: A comparison of three different systems
4. mRNA stability and plasmid copy number effects on gene expression from an inducible promoter system
5. Plasmids for the selection and analysis of prokaryotic promoters
6. Modification of mRNA secondary structure and alteration of expression of human interferon α1 in Escherichia coli
7. Inefficient translation initiation causes premature transcriptional termination in the LacZ gene
8. Stability of group I intron DNA in Escherichia coli and its potential application in a novel expression vector
9. Protein overproduction in Escherichia coli: RNA stabilization, cell disruption and recovery with a cross-flow microfiltration membrane
10. Intracellular degradation of recombinant proteins in relation to their location in Escherichia coli cells
11. Proteases and protein degradation in Escherichia coli
12. The mechanism and functions of ATP-dependent proteases in bacterial and animal cells
13. The influence of energy sources on proteolysis of a recombinant protein A in Escherichia coli
14. Immobilization and purification of enzymes with staphylococcal protein A gene fusion vectors
15. Regulation of Escherichia coli starvation sigma factor (σ(s)) by ClpXP protease
16. Engineering proteins to enhance their partition coefficients in aqueous two-phase systems
17. Enzymes - Laboratory-scale production
18. Factors influencing inclusion body formation in the production of a fused protein in Escherichia coli
19. Stabilization of a proteolytically sensitive cytoplasmic recombinant protein during transition to downstream processing
20. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
21. Response of guanosine tetraphosphate to glucose fluctuations in fed-batch cultivations of Escherichia coli
22. Rapid degradation of an abnormal protein in E. coli involves the chaperones GroEL and GroES
23. Process conditions affecting proteolysis of recombinant proteins in Escherichia coli
24. Metabolic growth rate control in Escherichia coli may be a consequence of subsaturation of the macromolecular biosynthetic apparatus with substrates and catalytic components
25. The use of fed-batch cultivation for achieving high cell densities in the production of a recombinant protein in E. coli
26. Synthesis of unique proteins at the onset of carbon starvation in Escherichia coli
27. The intracellular turnover of protein and nucleic acids and its role in biochemical differentiation
28. Proteolysis of fusion proteins: Stabilization and destabilization of staphylococcal protein A and E. coli β-galactosidase