Thymosin alpha-1; a natural peptide inhibits cellular proliferation, cell migration, the level of reactive oxygen species and promotes the activity of antioxidant enzymes in human lung epithelial adenocarcinoma cell line (A549)

Environmental Toxicology

Volumn 34, Issue 8, 2019, Pages 941-949

  Kharazmi Khorassani, Jasmin ^a   Asoodeh, Ahmad ^a  

^a FERDOWSI UNIVERSITY OF MASHHAD   (Iran)

Author keywords

antioxidant enzyme; cytotoxicity; reactive oxygen species; thymosin alpha 1

Indexed keywords

BIOLOGICAL ORGANS; CELL CULTURE; CELL DEATH; CELL PROLIFERATION; CYTOTOXICITY; DISEASES; ENZYMES; OXYGEN; PEPTIDES; RESPIRATORY SYSTEM;

ANTIOXIDANT ENZYME; ANTIPROLIFERATIVE EFFECT; CELLULAR PROLIFERATIONS; CONCENTRATION-DEPENDENT MANNERS; GLUTATHIONE PEROXIDASE; HUMAN LUNG CANCER CELLS; REACTIVE OXYGEN SPECIES; THYMOSIN ALPHA-1;

ANTIOXIDANTS;

CATALASE; GLUTATHIONE PEROXIDASE; REACTIVE OXYGEN METABOLITE; SUPEROXIDE DISMUTASE; THYMOSIN ALPHA1; ANTINEOPLASTIC AGENT; ANTIOXIDANT;

ANTIOXIDANT; APOPTOSIS; CANCER; CELL; ENZYME ACTIVITY; INHIBITION; PEPTIDE; PROTEIN; REACTIVE OXYGEN SPECIES;

A-549 CELL LINE; ANTIOXIDANT ACTIVITY; ANTIPROLIFERATIVE ACTIVITY; APOPTOSIS; ARTICLE; CELL CYCLE; CELL MIGRATION; CELL PROLIFERATION; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOTOXICITY; ENZYME ACTIVITY; GROWTH INHIBITION; HUMAN; HUMAN CELL; PRIORITY JOURNAL; CELL MOTION; DRUG EFFECT; METABOLISM;

A549 CELLS; ANTINEOPLASTIC AGENTS; ANTIOXIDANTS; APOPTOSIS; CATALASE; CELL MOVEMENT; CELL PROLIFERATION; GLUTATHIONE PEROXIDASE; HUMANS; REACTIVE OXYGEN SPECIES; SUPEROXIDE DISMUTASE; THYMALFASIN;

EID: 85065523917     PISSN: 15204081     EISSN: 15227278     Source Type: Journal    
DOI: 10.1002/tox.22765     Document Type: Article

References (57)

1. Bar-Or D, Carrick MM, Mains CW, Rael LT, Slone D, Brody EN. Sepsis, oxidative stress, and hypoxia: are there clues to better treatment? Redox Rep. 2015;20(5):193-197.
2. Maejima Y, Zablocki D, Sadoshima J. Oxidative stress and cardiac muscle. Muscle. United States: Elsevier. Vol 1; 2012:309-322.
3. Davies KJ. The broad spectrum of responses to oxidants in proliferating cells: a new paradigm for oxidative stress. IUBMB Life. 1999;48(1):41-47.
4. Bazhin AV, Philippov PP, Karakhanova S. Reactive oxygen species in cancer biology and anticancer therapy. Oxid Med Cell Longev. 2016;2016:1-2.
5. Huang WJ, Zhang XIA, Chen WW. Role of oxidative stress in Alzheimer's disease. Biomed Rep. 2016;4(5):519-522.
6. Blesa J, Trigo-Damas I, Quiroga-Varela A, Jackson-Lewis VR. Oxidative stress and Parkinson's disease. Front Neuroanat. 2015;9:91.
7. Sugamura K, Keaney JF Jr. Reactive oxygen species in cardiovascular disease. Free Radical Bio Med. 2011;51(5):978-992.
8. Poljsak B, Šuput D, Milisav I. Achieving the balance between ROS and antioxidants: when to use the synthetic antioxidants. Oxid Med Cell Longev. 2013;2013:1-11.
9. Kang D, Hamasaki N. Mitochondrial oxidative stress and mitochondrial DNA. Clin Chem Lab Med. 2003;41(10):1281-1288.
10. Behrend L, Henderson G, Zwacka R. Reactive Oxygen Species in Oncogenic Transformation. Biochem Soc Trans. 2003; 31(Pt 6):1441–1444.
11. Dionisi O, Galeotti T, Terranova T, Azzi A. Superoxide radicals and hydrogen peroxide formation in mitochondria from normal and neoplastic tissues. Biochim Biophys Acta Enzymology. 1975;403(2):292-300.
12. Oberley L, Bize I, Sahu S, Leuthauser SC, Gruber H. Superoxide dismutase activity of normal murine liver, regenerating liver, and H6 hepatoma. J Natl Cancer Inst. 1978;61(2):375-379.
13. Oberley LW, Buettner GR. Role of superoxide dismutase in cancer: a review. Cancer Res. 1979;39(4):1141-1149.
14. Bozzi A, Mavelli I, Agrò AF, et al. Enzyme defense against reactive oxygen derivatives. II. Erythrocytes and tumor cells. Mol Cell Biochem. 1976;10(1):11-16.
15. Lorestani S, Hashemy SI, Mojarad M, et al. Increased glutathione reductase expression and activity in colorectal cancer tissue samples: an investigational study in Mashhad. Iran Middle East J Cancer. 2018;9(2):99-104.
16. de Campos Z. Glutathione and glutathione peroxidase expression in breast cancer: an immunohistochemical and molecular study. Oncol Rep. 2013;30(3):1119-1128.
17. Walshe J, Serewko-Auret MM, Teakle N, et al. Inactivation of glutathione peroxidase activity contributes to UV-induced squamous cell carcinoma formation. Cancer Res. 2007;67(10):4751-4758.
18. Filaire E, Dupuis C, Galvaing G, et al. Lung cancer: what are the links with oxidative stress, physical activity and nutrition. Lung Cancer. 2013;82(3):383-389.
19. Hidir ESME, Cemek M, Sezer M, et al. High levels of oxidative stress in patients with advanced lung cancer. Respirology. 2008;13(1):112-116.
20. Goldstein AL, Low TL, McAdoo M, et al. Thymosin alpha1: isolation and sequence analysis of an immunologically active thymic polypeptide. Proc Natl Acad Sci USA. 1977;74(2):725-729.
21. Bach JF, Dardenne M, Goldstein AL, Guha A, White A. Appearance of T-cell markers in bone marrow rosette-forming cells after incubation with thymosin, a thymic hormone. Proc Natl Acad Sci. 1971;68(11):2734-2738.
22. Goldstein AL, Goldstein AL. From lab to bedside: emerging clinical applications of thymosin α1. Expert Opin Biol Therapy. 2009;9(5):593-608.
23. Li CH, Liu J, Wang FS. Thymosin alpha 1: biological activities, applications and genetic engineering production. Peptides. 2010;31(11):2151-2158.
24. Andreone P, Cursaro C, Gramenzi A, et al. A double-blind, placebo-controlled, pilot trial of thymosin alpha 1 for the treatment of chronic hepatitis C. Liver. 1996;16(3):207-210.
25. Fried MW, Shiffman ML, Reddy KR, et al. Peginterferon alfa-2a plus ribavirin for chronic hepatitis C virus infection. N Engl J Med. 2002;347(13):975-982.
26. McMichael AJ, Rowland-Jones SL. Cellular immune responses to HIV. Nature. 2001;410(6831):980-987.
27. Muzavir SR, Zahra SA, Ahmad A. An overview of purification, biological activities and therapeutic applications of thymosin alpha. Health. 2012;3:75-78.
28. Beuth J, Schierholz J, Mayer G. Thymosin α1 application augments immune response and down-regulates tumor weight and organ colonization in BALB/c-mice. Cancer Lett. 2000;159(1):9-13.
29. Qin Y, Chen FD, Zhou L, Gong XG, Han QF. Proliferative and anti-proliferative effects of thymosin α1 on cells are associated with manipulation of cellular ROS levels. Chem Biol Interact. 2009;180(3):383-388.
30. Guo Y, Chang H, Li J, et al. Thymosin alpha 1 suppresses proliferation and induces apoptosis in breast cancer cells through PTEN-mediated inhibition of PI3K/Akt/mTOR signaling pathway. Apoptosis. 2015;20(8):1109-1121.
31. Maurya DK, Nandakumar N, Devasagayam TPA. Anticancer property of gallic acid in A549, a human lung adenocarcinoma cell line, and possible mechanisms. J Clin Biochem Nutr. 2011;48(1):85-90.
32. Weydert CJ, Cullen JJ. Measurement of superoxide dismutase, catalase and glutathione peroxidase in cultured cells and tissue. Nat Protoc. 2010;5(1):51-66.
33. Claiborne A. Catalase Activity. CRC Handbook of Methods for Oxygen Radical Research. Boca Raton: CRC Press. 1986.
34. Anderson WL, Wetlaufer DB. A new method for disulfide analysis of peptides. Anal Biochem. 1975;67(2):493-502.
35. Lampugnani MG. Cell migration into a wounded area in vitro. Methods Mol Biol. 1999:177-182.
36. Stratil P, Klejdus B, Kubáň V. Determination of total content of phenolic compounds and their antioxidant activity in vegetables evaluation of spectrophotometric methods. J Agr Food Chem. 2006;54(3):607-616.
37. Ramonede BM, Tomas RP. Activation of protein kinase C for protection of cells against apoptosis induced by the immunosuppressor prodigiosin. Biochem Pharmacol. 2002;63(3):463-469.
38. Krishan A. Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining. J Cell Biol. 1975;66(1):188-193.
39. Lao X, Li B, Liu M, et al. A modified thymosin alpha 1 inhibits the growth of breast cancer both in vitro and in vivo: suppressment of cell proliferation, inducible cell apoptosis and enhancement of targeted anticancer effects. Apoptosis. 2015;20(10):1307-1320.
40. Naylor PH, Mutchnick MG. Immunotherapy for hepatitis B in the direct acting antiviral era: reevaluating the thymosin α1 efficacy trials in the light of a combination therapy approach. J Viral Hepatitis. 2018;25(1):4-9.
41. Camerini R, Garaci E. Historical review of thymosin α 1 in infectious diseases. Expert Opin Biol Therapy. 2015;15(sup 1):117-127.
42. Goldstein AL. History of the discovery of the thymosins. Ann N Y Acad Sci. 2007;1112(1):1-13.
43. Ma YL, Zheng Z, Li BD, et al. The anti-tumor efforts of thymosin alpha1 on tumor lysate-pulsed dendritic cells in colon cancer in vitro and in vivo. Chin J Cell Mol Immunol. 2007;23(11):1046-1049.
44. Lao X, Liu M, Chen J, Zheng H. A tumor-penetrating peptide modification enhances the antitumor activity of thymosin alpha 1. PLoS One. 2013;8(8):e72242.
45. Moody TW. Thymosin α1 as a chemopreventive agent in lung and breast cancer. Ann Ny Acad Sci. 2007;1112(1):297-304.
46. Moody TW, Tuthill C, Badamchian M, Goldstein AL. Thymosin α1 inhibits mammary carcinogenesis in Fisher rats. Peptides. 2002;23(5):1011-1014.
47. Wang F, Yu T, Zheng H, Lao X. Thymosin alpha1-Fc modulates the immune system and down-regulates the progression of melanoma and breast cancer with a prolonged half-life. Sci Rep. 2018;8(1):12351.
48. Shen X, Li Q, Wang F, et al. Generation of a novel long-acting thymosin alpha1-Fc fusion protein and its efficacy for the inhibition of breast cancer in vivo. Biomed Pharmacother. 2018;108:610-617.
49. Ni C, Wu P, Wu X, et al. Thymosin alpha1 enhanced cytotoxicity of iNKT cells against colon cancer via upregulating CD1d expression. Cancer Lett. 2015;356(2):579-588.
50. Yuan C, Zheng Y, Zhang B, et al. Thymosin α1 promotes the activation of myeloid-derived suppressor cells in a Lewis lung cancer model by upregulating arginase 1. Biochem Biophys Res Commun. 2015;464(1):249-255.
51. Fan YZ, Chang H, Yu Y, Liu J, Wang R. Thymosin α1 suppresses proliferation and induces apoptosis in human leukemia cell lines. Peptides. 2006;27(9):2165-2173.
52. Yf L. Thymalfasin (thymosin-alpha 1) therapy in patients with chronic hepatitis B. J Gastroen Hepatol. 2004;19(S6):S73-S75.
53. Qiu L, Zhang C, Zhang J, et al. Intraperitoneal co-administration of thymosin α-1 ameliorates streptozotocin-induced pancreatic lesions and diabetes in C57BL/6 mice. Int J Mol Med. 2009;23(5):597-602.
54. Lau BH, Li L, Yoon P. Thymic peptide protects vascular endothelial cells from hydrogen peroxide-induced oxidant injury. Life Sci. 1993;52(22):1787-1796.
55. Gökkuşu C, Ademoğlu E, Türkoğlu ÜM, Öz H, Öz F. Thymosin α1 protects liver and aorta from oxidative damage in atherosclerotic rabbits. Life Sci. 1996;59(13):1059-1067.
56. Malinda KM, Sidhu GS, Banaudha KK, et al. Thymosin α1 stimulates endothelial cell migration. Angiogenesis, and wound healing. J Immunol. 1998;160(2):1001-1006.
57. Palamara A, Bue M, Savini P. Thymosin alpha 1 inhibits Sendai virus replication: involvement of intracellular redox state. 6th International Expert Forum of Immunotherapy and Gene Therapy; London, England: International Medical Press. 1998.