A multiplex one-tube nested real time RT-PCR assay for simultaneous detection of respiratory syncytial virus, human rhinovirus and human metapneumovirus

Virology Journal

Volumn 15, Issue 1, 2018, Pages

  Feng, Zhi Shan ^a   Zhao, Li ^b,c   Wang, Ji ^c   Qiu, Fang Zhou ^b,c   Zhao, Meng Chuan ^a   Wang, Le ^a   Duan, Su Xia ^a   Zhang, Rui Qing ^b,c   Chen, Chen ^c   Qi, Ju Ju ^b,c   Fan, Tao ^b,c   Li, Gui Xia ^a   Ma, Xue Jun ^c  

^a Children's Hospital of Hebei Province   (China)

^b HEBEI MEDICAL UNIVERSITY   (China)

^c CHINESE CENTER FOR DISEASE CONTROL AND PREVENTION   (China)

Author keywords

Detection; HMPV; HRV; LNA; Multiplex one tube nested real time RT PCR; RSV

Indexed keywords

LOCKED NUCLEIC ACID;

ARTICLE; CHILD; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; CROSS REACTION; DIAGNOSTIC ACCURACY; DIAGNOSTIC TEST ACCURACY STUDY; DIAGNOSTIC VALUE; FEMALE; HUMAN; HUMAN METAPNEUMOVIRUS; HUMAN RESPIRATORY SYNCYTIAL VIRUS; HUMAN RHINOVIRUS; INFANT; INTERMETHOD COMPARISON; LIMIT OF DETECTION; MAJOR CLINICAL STUDY; MALE; MULTIPLEX ONE TUBE NESTED REAL TIME POLYMERASE CHAIN REACTION; NASOPHARYNGEAL ASPIRATION; NESTED POLYMERASE CHAIN REACTION; NONHUMAN; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; RESPIRATORY TRACT INFECTION; SENSITIVITY AND SPECIFICITY; VIRUS DETECTION; ACUTE DISEASE; EVALUATION STUDY; GENETICS; ISOLATION AND PURIFICATION; METAPNEUMOVIRUS; MULTIPLEX POLYMERASE CHAIN REACTION; NASOPHARYNX; PARAMYXOVIRUS INFECTION; PICORNAVIRUS INFECTION; PRESCHOOL CHILD; RESPIRATORY SYNCYTIAL VIRUS INFECTION; RHINOVIRUS; VIROLOGY;

ACUTE DISEASE; CHILD; CHILD, PRESCHOOL; FEMALE; HUMANS; INFANT; MALE; METAPNEUMOVIRUS; MULTIPLEX POLYMERASE CHAIN REACTION; NASOPHARYNX; PARAMYXOVIRIDAE INFECTIONS; PICORNAVIRIDAE INFECTIONS; REPRODUCIBILITY OF RESULTS; RESPIRATORY SYNCYTIAL VIRUS INFECTIONS; RESPIRATORY SYNCYTIAL VIRUS, HUMAN; RHINOVIRUS; SENSITIVITY AND SPECIFICITY;

EID: 85055622448     PISSN: None     EISSN: 1743422X     Source Type: Journal    
DOI: 10.1186/s12985-018-1061-0     Document Type: Article

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