Development of three multiplex RT-PCR assays for the detection of 12 respiratory RNA viruses

Journal of Virological Methods

Volumn 126, Issue 1-2, 2005, Pages 53-63

  Bellau Pujol, S ^a   Vabret, A ^a   Legrand, L ^a   Dina, J ^a   Gouarin, S ^a   Petitjean Lecherbonnier, J ^a   Pozzetto, B ^b   Ginevra, C ^b   Freymuth, F ^a  

^a CAEN UNIVERSITY HOSPITAL   (France)

^b UNIVERSITY HOSPITAL OF SAINT ETIENNE   (France)

Author keywords

Human coronavirus; Human metapneumovirus; Influenza virus; Multiplex RT PCR assay; Parainfluenza virus; Respiratory syncytial virus; Rhinovirus

Indexed keywords

ARTICLE; CONTROLLED STUDY; CORONAVIRUS; DIAGNOSTIC ACCURACY; IMMUNOFLUORESCENCE; INFLUENZA VIRUS A; INFLUENZA VIRUS B; INFLUENZA VIRUS C; METAPNEUMOVIRUS; NONHUMAN; PARAINFLUENZA VIRUS 1; PARAINFLUENZA VIRUS 2; PARAINFLUENZA VIRUS 3; PARAINFLUENZA VIRUS 4; PRIORITY JOURNAL; RESPIRATORY SYNCYTIAL PNEUMOVIRUS; RETROSPECTIVE STUDY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RHINOVIRUS; RNA VIRUS; VIRUS DETECTION; VIRUS ISOLATION;

CORONAVIRUS; HUMAN CORONAVIRUS; HUMAN METAPNEUMOVIRUS; HUMAN RESPIRATORY SYNCYTIAL VIRUS; INFLUENZA VIRUS; INFLUENZAVIRUS A; METAPNEUMOVIRUS; RESPIRATORY SYNCYTIAL VIRUS; RHINOVIRUS; RICE STRIPE VIRUS; RNA VIRUSES; SYNCYTIAL VIRUS;

EID: 17644402413     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2005.01.020     Document Type: Article

References (50)

1. J.C. Aguilar, M.P. Perez-Brena, M.L. Garcia, N. Cruz, D.D. Erdman, and J.E. Echevarria Detection and identification of human parainfluenza viruses 1, 2, 3, and 4 in clinical samples of pediatric patients by multiplex reverse transcription-PCR J. Clin. Microbiol. 38 3 2000 1191 1195
2. G. Boivin, G. De Serres, S. Cote, R. Gilca, Y. Abed, L. Rochette, M.G. Bergeron, and P. Dery Human metapneumovirus infections in hospitalized children Emerg. Infect. Dis. 9 6 2003 634 640
3. J. Brouard, F. Freymuth, A. Vabret, M. Jokic, B. Guillois, and J.F. Duhamel Viral co-infections in immunocompetent infants with bronchiolitis: prospective epidemiologic study Arch. Pediatr. 7 Suppl. 3 2000 531s 535s
4. P.A. Cane, and C.R. Pringle Respiratory syncytial virus heterogeneity during an epidemic: analysis by limited nucleotide sequencing (SH gene) and restriction mapping (N gene) J. Gen. Virol. 72 2 1991 349 357
5. A.E. Casiano-Colon, B.B. Hulbert, T.K. Mayer, E.E. Walsh, and A.R. Falsey Lack of sensitivity of rapid antigen tests for the diagnosis of respiratory syncytial virus infection in adults J. Clin. Virol. 28 2 2003 169 174
6. M.T. Coiras, P. Perez-Brena, M.L. Garcia, and I. Casas Simultaneous detection of influenza A, B, and C viruses, respiratory syncytial virus, and adenoviruses in clinical samples by multiplex reverse transcription nested-PCR assay J. Med. Virol. 69 1 2003 132 144
7. M.T. Coiras, J.C. Aguilar, M.L. Garcia, I. Casas, and P. Perez-Brena Simultaneous detection of fourteen respiratory viruses in clinical specimens by two multiplex reverse transcription nested-PCR assays J. Med. Virol. 72 3 2004 484 495
8. K.E. Dingle, D. Crook, and K. Jeffery Stable and noncompetitive RNA internal control for routine clinical diagnostic reverse transcription-PCR J. Clin. Microbiol. 42 3 2004 1003 1011
9. J.C. Donofrio, J.D. Coonrod, J.N. Davidson, and R.F. Betts Detection of influenza A and B in respiratory secretions with the polymerase chain reaction PCR Methods Appl. 1 4 1992 263 268
10. J.E. Echevarria, D.D. Erdman, E.M. Swierkosz, B.P. Holloway, and L.J. Anderson Simultaneous detection and identification of human parainfluenza viruses 1, 2, and 3 from clinical samples by multiplex PCR J. Clin. Microbiol. 36 5 1998 1388 1391
11. E.M. Elnifro, A.M. Ashshi, R.J. Cooper, and P.E. Klapper Multiplex PCR: optimization and application in diagnostic virology Clin. Microbiol. Rev. 13 4 2000 559 570
12. G. Eugene-Ruellan, F. Freymuth, C. Bahloul, H. Badrane, A. Vabret, and N. Tordo Detection of respiratory syncytial virus A and B and parainfluenza virus 3 sequences in respiratory tracts of infants by a single PCR with primers targeted to the L-polymerase gene and differential hybridization J. Clin. Microbiol. 36 3 1998 796 801
13. J. Fan, K.J. Henrickson, and L.L. Savatski Rapid simultaneous diagnosis of infections with respiratory syncytial viruses A and B, influenza viruses A and B, and human parainfluenza virus types 1, 2, and 3 by multiplex quantitative reverse transcription-polymerase chain reaction-enzyme hybridization assay (Hexaplex) Clin. Infect. Dis. 26 6 1998 1397 1402
14. F. Freymuth, M. Quibriac, J. Petitjean, F. Daon, and M.L. Amiel Viruses responsible for respiratory infections in pediatrics. Evaluation of 3480 nasal aspirates performed in children over a 6-year period Ann. Pediatr. (Paris) 34 7 1987 493 501
15. F. Freymuth, G. Eugene, A. Vabret, J. Petitjean, E. Gennetay, J. Brouard, J.F. Duhamel, and B. Guillois Detection of respiratory syncytial virus by reverse transcription-PCR and hybridization with a DNA enzyme immunoassay J. Clin. Microbiol. 33 12 1995 3352 3355 (erratum 1996;34:1601)
16. F. Freymuth, A. Vabret, F. Galateau-Salle, J. Ferey, G. Eugene, J. Petitjean, E. Gennetay, J. Brouard, M. Jokik, J.F. Duhamel, and B. Guillois Detection of respiratory syncytial virus, parainfluenza virus 3, adenovirus and rhinovirus sequences in respiratory tract of infants by polymerase chain reaction and hybridization Clin. Diagn. Virol. 8 1 1997 31 40
17. F. Freymuth, A. Vabret, L. Legrand, N. Eterradossi, F. Lafay-Delaire, J. Brouard, and B. Guillois Presence of the new human metapneumovirus in French children with bronchiolitis Pediatr. Infect. Dis. J. 22 1 2003 92 94
18. F. Freymuth Infections respiratoires à adénovirus F. Freymuth Infections Virales Respiratoires-Tome 2 2001 Elsevier Paris 107 119
19. F. Freymuth, A. Vabret, L. Legrand, N. Eterradossi, F. Lafay-Delaire, J. Brouard, and B. Guillois Presence of the new human metapneumovirus in French children with bronchiolitis Pediatr. Infect. Dis. J. 22 1 2003 92 94
20. P.S. Gardner, and J. McQuillin Application of immunofluorescent antibody technique in rapid diagnosis of respiratory syncytial virus infection Br. Med. J. 3 614 1968 340 343
21. L.L. Gilbert, A. Dakhama, B.M. Bone, E.E. Thomas, and R.G. Hegele Diagnosis of viral respiratory tract infections in children by using a reverse transcription-PCR panel J. Clin. Microbiol. 34 1 1996 140 143
22. W.P. Glezen, A. Paredes, and L.H. Taber Influenza in children. Relationship to other respiratory agents JAMA 243 13 1980 1345 1349
23. B. Grondahl, W. Puppe, A. Hoppe, I. Kuhne, J.A. Weigl, and H.J. Schmitt Rapid identification of nine microorganisms causing acute respiratory tract infections by single-tube multiplex reverse transcription-PCR: feasibility study J. Clin. Microbiol. 37 1 1999 1 7
24. M. Gueudin, A. Vabret, J. Petitjean, S. Gouarin, J. Brouard, and F. Freymuth Quantitation of respiratory syncytial virus RNA in nasal aspirates of children by real-time RT-PCR assay J. Virol. Methods 109 2003 39 45
25. V. Guittet, J. Brouard, A. Vabret, F. Lafay, B. Guillois, J.F. Duhamel, and F. Freymuth Rhinovirus and acute respiratory infections in hospitalized children. Retrospective study 1998-2000 Arch. Pediatr. 10 5 2003 417 423
26. M. Hindiyeh, D.R. Hillyard, and K.C. Carroll Evaluation of the Prodesse Hexaplex multiplex PCR assay for direct detection of seven respiratory viruses in clinical specimens Am. J. Clin. Pathol. 116 2 2001 218 224
27. T. Jartti, P. Lehtinen, T. Vuorinen, M. Koskenvuo, and O. Ruuskanen Persistence of rhinovirus and enterovirus RNA after acute respiratory illness in children J. Med. Virol. 72 4 2004 695 699
28. R.A. Karron, J.L. Froehlich, L. Bobo, R.B. Belshe, and R.H. Yolken Rapid detection of parainfluenza virus type 3 RNA in respiratory specimens: use of reverse transcription-PCR-enzyme immunoassay J. Clin. Microbiol. 32 2 1994 484 488
29. S.C. Kehl, K.J. Henrickson, W. Hua, and J. Fan Evaluation of the Hexaplex assay for detection of respiratory viruses in children J. Clin. Microbiol. 39 5 2001 1696 1701
30. S.W. Lindquist, A. Darnule, A. Istas, and G.J. Demmler Parainfluenza virus type 4 infections in pediatric patients Pediatr. Infect. Dis. J. 16 1 1997 34 38
31. L. Liolios, A. Jenney, D. Spelman, T. Kotsimbos, M. Catton, and S. Wesselingh Comparison of a multiplex reverse transcription-PCR-enzyme hybridization assay with conventional viral culture and immunofluorescence techniques for the detection of seven viral respiratory pathogens J. Clin. Microbiol. 39 8 2001 2779 2783
32. A.J. Maletzky, M.K. Cooney, R. Luce, G.E. Kenny, and J.T. Grayston Epidemiology of viral and mycoplasmal agents associated with childhood lower respiratory illness in a civilian population J. Pediatr. 78 3 1971 407 414
33. J.C. Manuguerra, C. Hannoun, and M. Aymard Influenza C virus infection in France J. Infect. 24 1 1992 91 99
34. H.C. Meissner, S.A. Murray, M.A. Kiernan, D.R. Snydman, and K. McIntosh A simultaneous outbreak of respiratory syncytial virus and parainfluenza virus type 3 in a newborn nursery J. Pediatr. 104 5 1984 680 684
35. N. Nakabayashi, S. Hahata, and E. Masuhara Studies on dental self-curing resins. XV. Application of nitrene to reactive adhesive with a tooth J. Biomed. Mater. Res. 11 3 1977 395 404
36. C. Osiowy Direct detection of respiratory syncytial virus, parainfluenza virus, and adenovirus in clinical respiratory specimens by a multiplex reverse transcription-PCR assay J. Clin. Microbiol. 36 11 1998 3149 3154
37. B. Portnoy, H.L. Eckert, B. Hanes, and M.A. Salvatore Multiple respiratory virus infections in hospitalized children Am. J. Epidemiol. 82 3 1965 262 272
38. W. Puppe, J.A. Weigl, G. Aron, B. Grondahl, H.J. Schmitt, H.G. Niesters, and J. Groen Evaluation of a multiplex reverse transcriptase PCR ELISA for the detection of nine respiratory tract pathogens J. Clin. Virol. 30 2 2004 165 174
39. C. Savolainen, M.N. Mulders, and T. Hovi Phylogenetic analysis of rhinovirus isolates collected during successive epidemic seasons Virus Res. 85 1 2002 41 46
40. C. Savolainen, S. Blomqvist, and T. Hovi Human rhinoviruses Paediatr. Respir. Rev. 4 2 2003 91 98
41. M.W. Syrmis, D.M. Whiley, M. Thomas, I.M. Mackay, J. Williamson, D.J. Siebert, M.D. Nissen, and T.P. Sloots A sensitive, specific, and cost-effective multiplex reverse transcriptase-PCR assay for the detection of seven common respiratory viruses in respiratory samples J. Mol. Diagn. 6 2 2004 125 131
42. K.E. Templeton, S.A. Scheltinga, M.F. Beersma, A.C. Kroes, and E.C. Claas Rapid and sensitive method using multiplex real-time PCR for diagnosis of infections by influenza a and influenza B viruses, respiratory syncytial virus, and parainfluenza viruses 1, 2, 3, and 4 J. Clin. Microbiol. 42 4 2004 1564 1569
43. D.A. Tristram, R.W. Miller, J.A. McMillan, and L.B. Weiner Simultaneous infection with respiratory syncytial virus and other respiratory pathogens Am. J. Dis. Child. 142 8 1988 834 836
44. A. Vabret, J. Brouard, J. Petitjean, G. Eugene-Ruellan, and F. Freymuth Human coronavirus infections: importance and diagnosis Presse Med. 27 35 1998 1813 1817
45. A. Vabret, G. Sapin, B. Lezin, A. Mosnier, J.-M. Cohen, and L. Burnouf Comparaison of three non-nested RT-PCR for the detection of influenza A viruses J. Clin. Virol. 17 2000 167 175
46. A. Vabret, F. Mouthon, T. Mourez, S. Gouarin, J. Petitjean, and F. Freymuth Direct diagnosis of human respiratory coronaviruses 229E and OC43 by the polymerase chain reaction J. Virol. Methods 97 1-2 2001 59 66
47. A. Vabret, T. Mourez, S. Gouarin, J. Petitjean, and F. Freymuth An outbreak of coronavirus OC43 respiratory infection in Normandy, France Clin. Infect. Dis. 36 8 2003 985 989
48. W.M. Valenti, T.A. Clarke, C.B. Hall, M.A. Menegus, and D.L. Shapiro Concurrent outbreaks of rhinovirus and respiratory syncytial virus in an intensive care nursery: epidemiology and associated risk factors J. Pediatr. 100 5 1982 722 726
49. B.G. Van Den Hoogen, D.M. Osterhaus, and R.A. Fouchier Clinical impact and diagnosis of human metapneumovirus infection Pediatr. Infect. Dis. J. 23 1 Suppl 2004 S25 S32
50. W.D. Zhang, and D.H. Evans Detection and identification of human influenza viruses by the polymerase chain reaction J. Virol. Methods 33 1-2 1991 165 189