Simultaneous detection of respiratory syncytial virus and human metapneumovirus by one-step multiplex real-time RT-PCR in patients with respiratory symptoms

BMC Pediatrics

Volumn 17, Issue 1, 2017, Pages

  You, Huey Ling ^a,b   Chang, Shun Jen ^c   Yu, Hong Ren ^d   Li, Chia Chin ^a   Chen, Chang Han ^a,e   Liao, Wei Ting ^f,g  

^a CHANG GUNG MEMORIAL HOSPITAL   (Taiwan)

^b FOOYIN UNIVERSITY   (Taiwan)

^c NATIONAL UNIVERSITY OF KAOHSIUNG   (Taiwan)

^d CHANG GUNG UNIVERSITY COLLEGE OF MEDICINE   (Taiwan)

^e NATIONAL CHI NAN UNIVERSITY   (Taiwan)

^f KAOHSIUNG MEDICAL UNIVERSITY   (Taiwan)

^g NATIONAL SUN YAT SEN UNIVERSITY   (Taiwan)

Author keywords

HMPV; Real time RT PCR; Respiratory tract infection; RSV

Indexed keywords

NUCLEOPROTEIN;

ADOLESCENT; ARTICLE; CHILD; CONTROLLED STUDY; FEMALE; FUSION GENE; HOSPITALIZATION; HUMAN; HUMAN METAPNEUMOVIRUS; HUMAN RESPIRATORY SYNCYTIAL VIRUS; INFANT; LIMIT OF DETECTION; LIMIT OF QUANTITATION; MAJOR CLINICAL STUDY; MALE; NASOPHARYNGEAL ASPIRATION; NEWBORN; NONHUMAN; NUCLEOPROTEIN GENE; ONE STEP TRIPLEX REAL TIME POLYMERASE CHAIN REACTION; PROCESS OPTIMIZATION; REAL TIME POLYMERASE CHAIN REACTION; RELIABILITY; REPRODUCIBILITY; RESPIRATORY TRACT INFECTION; SENSITIVITY AND SPECIFICITY; THORAX RADIOGRAPHY; VIRUS CULTURE; VIRUS DETECTION; CLINICAL TRIAL; DIFFERENTIAL DIAGNOSIS; ISOLATION AND PURIFICATION; METAPNEUMOVIRUS; MULTIPLEX POLYMERASE CHAIN REACTION; PARAMYXOVIRUS INFECTION; PRESCHOOL CHILD; PROCEDURES; PROSPECTIVE STUDY; RESPIRATORY SYNCYTIAL VIRUS INFECTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VIROLOGY;

CHILD, PRESCHOOL; DIAGNOSIS, DIFFERENTIAL; FEMALE; HUMANS; INFANT; INFANT, NEWBORN; MALE; METAPNEUMOVIRUS; MULTIPLEX POLYMERASE CHAIN REACTION; PARAMYXOVIRIDAE INFECTIONS; PROSPECTIVE STUDIES; REAL-TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; RESPIRATORY SYNCYTIAL VIRUS INFECTIONS; RESPIRATORY TRACT INFECTIONS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

EID: 85016164701     PISSN: None     EISSN: 14712431     Source Type: Journal    
DOI: 10.1186/s12887-017-0843-7     Document Type: Article

References (20)

1. Paget SP, Andresen DN, Kesson AM, Egan JR. Comparison of human metapneumovirus and respiratory syncytial virus in children admitted to a paediatric intensive care unit. J Paediatr Child Health. 2011;47(10):737-41.
2. Berkley JA, Munywoki P, Ngama M, Kazungu S, Abwao J, Bett A, Lassauniere R, Kresfelder T, Cane PA, Venter M, et al. Viral etiology of severe pneumonia among Kenyan infants and children. JAMA. 2010;303(20):2051-7.
3. Nair H, Nokes DJ, Gessner BD, Dherani M, Madhi SA, Singleton RJ, O'Brien KL, Roca A, Wright PF, Bruce N, et al. Global burden of acute lower respiratory infections due to respiratory syncytial virus in young children: a systematic review and meta-analysis. Lancet. 2010;375(9725):1545-55.
4. Forgie IM, O'Neill KP, Lloyd-Evans N, Leinonen M, Campbell H, Whittle HC, Greenwood BM. Etiology of acute lower respiratory tract infections in Gambian children: I. Acute lower respiratory tract infections in infants presenting at the hospital. Pediatr Infect Dis J. 1991;10(1):33-41.
5. Nagakumar P, Doull I. Current therapy for bronchiolitis. Arch Dis Child. 2012;97(9):827-30.
6. Manoha C, Espinosa S, Aho SL, Huet F, Pothier P. Epidemiological and clinical features of hMPV, RSV and RVs infections in young children. J Clin Virol. 2007;38(3):221-6.
7. van den Hoogen BG, de Jong JC, Groen J, Kuiken T, de Groot R, Fouchier RA, Osterhaus AD. A newly discovered human pneumovirus isolated from young children with respiratory tract disease. Nat Med. 2001;7(6):719-24.
8. Papenburg J, Boivin G. The distinguishing features of human metapneumovirus and respiratory syncytial virus. Rev Med Virol. 2010;20(4):245-60.
9. van den Hoogen BG, Osterhaus DM, Fouchier RA. Clinical impact and diagnosis of human metapneumovirus infection. Pediatr Infect Dis J. 2004;23(1 Suppl):S25-32.
10. Schildgen V, van den Hoogen B, Fouchier R, Tripp RA, Alvarez R, Manoha C, Williams J, Schildgen O. Human Metapneumovirus: lessons learned over the first decade. Clin Microbiol Rev. 2011;24(4):734-54.
11. Louie JK, Schnurr DP, Pan CY, Kiang D, Carter C, Tougaw S, Ventura J, Norman A, Belmusto V, Rosenberg J, et al. A summer outbreak of human metapneumovirus infection in a long-term-care facility. J Infect Dis. 2007;196(5):705-8.
12. Wilkesmann A, Schildgen O, Eis-Hubinger AM, Geikowski T, Glatzel T, Lentze MJ, Bode U, Simon A. Human metapneumovirus infections cause similar symptoms and clinical severity as respiratory syncytial virus infections. Eur J Pediatr. 2006;165(7):467-75.
13. Kahn JS. Epidemiology of human metapneumovirus. Clin Microbiol Rev. 2006;19(3):546-57.
14. Muller A, Tillmann RL, Muller A, Simon A, Schildgen O. Stability of human metapneumovirus and human coronavirus NL63 on medical instruments and in the patient environment. J Hosp Infect. 2008;69(4):406-8.
15. Peret TC, Boivin G, Li Y, Couillard M, Humphrey C, Osterhaus AD, Erdman DD, Anderson LJ. Characterization of human metapneumoviruses isolated from patients in North America. J Infect Dis. 2002;185(11):1660-3.
16. Boivin G, Abed Y, Pelletier G, Ruel L, Moisan D, Cote S, Peret TC, Erdman DD, Anderson LJ. Virological features and clinical manifestations associated with human metapneumovirus: a new paramyxovirus responsible for acute respiratory-tract infections in all age groups. J Infect Dis. 2002;186(9):1330-4.
17. Williams JV, Harris PA, Tollefson SJ, Halburnt-Rush LL, Pingsterhaus JM, Edwards KM, Wright PF, Crowe Jr JE. Human metapneumovirus and lower respiratory tract disease in otherwise healthy infants and children. N Engl J Med. 2004;350(5):443-50.
18. Zhang Y, Wei Y, Li J, Li J. Development and optimization of a direct plaque assay for human and avian metapneumoviruses. J Virol Methods. 2012;185(1):61-8.
19. Holland PM, Abramson RD, Watson R, Gelfand DH. Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase. Proc Natl Acad Sci U S A. 1991;88(16):7276-80.
20. Jokela P, Piiparinen H, Luiro K, Lappalainen M. Detection of human metapneumovirus and respiratory syncytial virus by duplex real-time RT-PCR assay in comparison with direct fluorescent assay. Clin Microbiol Infect. 2010;16(10):1568-73.