Cytotoxic effects of heated tobacco products (HTP) on human bronchial epithelial cells

Tobacco Control

Volumn 27, Issue , 2018, Pages s26-s29

  Leigh, Noel J ^a   Tran, Phillip L ^a   O'Connor, Richard J ^a   Goniewicz, MacIej Lukasz ^a  

^a ROSWELL PARK CANCER INSTITUTE   (United States)

Author keywords

nicotine; non cigarette tobacco products; toxicology

Indexed keywords

CYTOKINE;

BRONCHUS; CELL CULTURE; CELL SURVIVAL; COMPARATIVE STUDY; DRUG EFFECT; EPITHELIUM CELL; HUMAN; METABOLISM; PHYSIOLOGY; SMOKE; TOBACCO;

BRONCHI; CELL SURVIVAL; CELLS, CULTURED; CYTOKINES; EPITHELIAL CELLS; HUMANS; SMOKE; TOBACCO PRODUCTS;

EID: 85053165136     PISSN: 09644563     EISSN: 14683318     Source Type: Journal    
DOI: 10.1136/tobaccocontrol-2018-054317     Document Type: Article

References (19)

1. Caraballo RS, Pederson LL, Gupta N. New tobacco products: do smokers like them? Tob Control 2006;15:39-44.
2. Smith MR, Clark B, Lüdicke F, et al. Evaluation of the Tobacco Heating System 2. 2. Part 1: description of the system and the scientific assessment program. Regul Toxicol Pharmacol 2016;81:S17-26.
3. Schaller JP, Keller D, Poget L, et al. Evaluation of the Tobacco Heating System 2. 2. Part 2: chemical composition, genotoxicity, cytotoxicity, and physical properties of the aerosol. Regul Toxicol Pharmacol 2016;81:S27-47.
4. Zanetti F, Titz B, Sewer A, et al. Comparative systems toxicology analysis of cigarette smoke and aerosol from a candidate modified risk tobacco product in organotypic human gingival epithelial cultures: A 3-day repeated exposure study. Food Chem Toxicol 2017;101:15-35.
5. Wong ET, Kogel U, Veljkovic E, et al. Evaluation of the Tobacco Heating System 2. 2. Part 4: 90-day OECD 413 rat inhalation study with systems toxicology endpoints demonstrates reduced exposure effects compared with cigarette smoke. Regul Toxicol Pharmacol 2016;81:S59-S81.
6. Zanetti F, Sewer A, Mathis C, et al. Systems toxicology assessment of the biological impact of a candidate modified risk tobacco product on human organotypic oral epithelial cultures. Chem Res Toxicol 2016;29:1252-69.
7. Leigh NJ, Palumbo MN, Marino AM, et al. Tobacco-specific nitrosamines (TSNA) in heated tobacco product IQOS. Tob Control 2018;27:S37-8.
8. Leigh NJ, Lawton RI, Hershberger PA, et al. Flavourings significantly affect inhalation toxicity of aerosol generated from electronic nicotine delivery systems (ENDS). Tob Control 2016;25:ii81-7.
9. Repetto G, del Peso A, Zurita JL. Neutral red uptake assay for the estimation of cell viability/cytotoxicity. Nat Protoc 2008;3:1125-31.
10. Strober W. Trypan blue exclusion test of cell viability. Curr Protoc Immunol 2001;Appendix 3:A3. B. 1-3.
11. Kim V, Rogers TJ, Criner GJ. New concepts in the pathobiology of chronic obstructive pulmonary disease. Proc Am Thorac Soc 2008;5:478-85.
12. Lappalainen U, Whitsett JA, Wert SE, et al. Interleukin-1beta causes pulmonary inflammation, emphysema, and airway remodeling in the adult murine lung. Am J Respir Cell Mol Biol 2005;32:311-8.
13. Barnes PJ. Immunology of asthma and chronic obstructive pulmonary disease. Nat Rev Immunol 2008;8:183-92.
14. Al-Alwan LA, Chang Y, Mogas A, et al. Differential roles of CXCL2 and CXCL3 and their receptors in regulating normal and asthmatic airway smooth muscle cell migration. J Immunol 2013;191:2731-41.
15. Yoshikawa M, Wada K, Yoshimura T, et al. Increased CXCL10 expression in nasal fibroblasts from patients with refractory chronic rhinosinusitis and asthma. Allergol Int 2013;62:495-502.
16. Moazed F, Chun L, Matthay MA, et al. Assessment of industry data on pulmonary and immunosuppressive effects of IQOS. Tob Control 2018:1-6.
17. Rock KL, Kono H. The inflammatory response to cell death. Annu Rev Pathol 2008;3:99-126.
18. Johnson MD, Schilz J, Djordjevic MV, et al. Evaluation of in vitro assays for assessing the toxicity of cigarette smoke and smokeless tobacco. Cancer Epidemiol Biomarkers Prev 2009;18:3263-304.
19. Lüdicke F, Picavet P, Baker G, et al. Effects of switching to the tobacco heating system 2. 2 menthol, smoking abstinence, or continued cigarette smoking on biomarkers of exposure: A randomized, controlled, open-label, multicenter study in sequential confinement and ambulatory settings (Part 1). Nicotine Tob Res 2018;20:161-72.