Use of the REVERT ® total protein stain as a loading control demonstrates significant benefits over the use of housekeeping proteins when analyzing brain homogenates by Western blot: An analysis of samples representing different gonadal hormone states

Molecular and Cellular Endocrinology

Volumn 473, Issue , 2018, Pages 156-165

  Kirshner, Z Z ^a   Gibbs, R B ^a  

^a UNIVERSITY OF PITTSBURGH   (United States)

Author keywords

Accelerated ovarian failure; Estradiol; Frontal cortex; Hippocampus; Menopause; Striatum; VCD

Indexed keywords

ALPHA TUBULIN; ANDROSTENEDIONE; BETA ACTIN; ESTRADIOL; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; HOUSEKEEPING PROTEIN; PROTEIN; SEX HORMONE; TESTOSTERONE; UNCLASSIFIED DRUG; HORMONE;

ADULT; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BRAIN HOMOGENATE; CORPUS STRIATUM; DIESTRUS; FEMALE; FLUORESCENCE ANALYSIS; FRONTAL CORTEX; GEL; HIPPOCAMPUS; IMMUNODETECTION; MENOPAUSE; NONHUMAN; OVARIECTOMY; PRIORITY JOURNAL; PROESTRUS; PROTEIN EXPRESSION; RAT; RELIABILITY; SENSITIVITY ANALYSIS; STAINING; TOTAL PROTEIN STAIN; WESTERN BLOTTING; YOUNG ADULT; ANIMAL; BLOOD; BRAIN; ESSENTIAL GENE; GONAD; METABOLISM; MOLECULAR WEIGHT; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROCEDURES; REPRODUCIBILITY; SPRAGUE DAWLEY RAT;

ANIMALS; BLOTTING, WESTERN; BRAIN; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FEMALE; GENES, ESSENTIAL; GONADS; HORMONES; MOLECULAR WEIGHT; PROTEINS; RATS, SPRAGUE-DAWLEY; REPRODUCIBILITY OF RESULTS; STAINING AND LABELING;

EID: 85045447453     PISSN: 03037207     EISSN: 18728057     Source Type: Journal    
DOI: 10.1016/j.mce.2018.01.015     Document Type: Article

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