A microsomal based method to detect aromatase activity in different brain regions of the rat using ultra performance liquid chromatography–mass spectrometry

Journal of Steroid Biochemistry and Molecular Biology

Volumn 163, Issue , 2016, Pages 113-120

  Li, Junyi ^a   Oberly, Patrick J ^a   Poloyac, Samuel M ^a   Gibbs, Robert B ^a  

^a UNIVERSITY OF PITTSBURGH   (United States)

Author keywords

CYP19A1; Estradiol; Local estrogen production; Mass spectroscopy

Indexed keywords

ANASTROZOLE; AROMATASE; ESTRADIOL; MESSENGER RNA; TESTOSTERONE; AROMATASE INHIBITOR; CYTOCHROME P450 1A1; NITRILE; TRIAZOLE DERIVATIVE;

ACCURACY; AMYGDALA; ANIMAL TISSUE; ARTICLE; CALIBRATION; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME INHIBITION; ENZYME KINETIC ASSAY; ENZYME KINETICS; ENZYME LOCALIZATION; FEMALE; FRONTAL CORTEX; HIPPOCAMPUS; HORMONE SYNTHESIS; INCUBATION TIME; MASS SPECTROMETRY; MICROSOME; NONHUMAN; OVARY CELL; PREOPTIC AREA; RAT; ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY; ANIMAL; BRAIN CHEMISTRY; CHEMISTRY; ENZYMOLOGY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; KINETICS; LIMIT OF DETECTION; MALE; METABOLISM; OVARY; PROCEDURES; REPRODUCIBILITY; SPRAGUE DAWLEY RAT;

AMYGDALA; ANIMALS; AROMATASE; AROMATASE INHIBITORS; BRAIN CHEMISTRY; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CYTOCHROME P-450 CYP1A1; ESTRADIOL; FEMALE; HIPPOCAMPUS; KINETICS; LIMIT OF DETECTION; MALE; MICROSOMES; NITRILES; OVARY; PREOPTIC AREA; RATS; RATS, SPRAGUE-DAWLEY; REPRODUCIBILITY OF RESULTS; TESTOSTERONE; TRIAZOLES;

EID: 84964587577     PISSN: 09600760     EISSN: 18791220     Source Type: Journal    
DOI: 10.1016/j.jsbmb.2016.04.013     Document Type: Article

References (41)

1. [1] Daniel, J.M., et al. Estrogen enhances performance of female rats during acquisition of a radial arm maze. Horm. Behav. 32:3 (1997), 217–225.
2. [2] Bimonte, H.A., Denenberg, V.H., Estradiol facilitates performance as working memory load increases. Psychoneuroendocrinology 24:2 (1999), 161–173.
3. [3] Luine, V.N., Jacome, L.F., Maclusky, N.J., Rapid enhancement of visual and place memory by estrogens in rats. Endocrinology 144:7 (2003), 2836–2844.
4. [4] Frye, C.A., Duffy, C.K., Walf, A.A., Estrogens and progestins enhance spatial learning of intact and ovariectomized rats in the object placement task. Neurobiol. Learn Mem. 88:2 (2007), 208–216.
5. [5] Gresack, J.E., Frick, K.M., Post-training estrogen enhances spatial and object memory consolidation in female mice. Pharmacol. Biochem. Behav. 84:1 (2006), 112–119.
6. [6] Sherwin, B.B., Henry, J.F., Brain aging modulates the neuroprotective effects of estrogen on selective aspects of cognition in women: a critical review. Front. Neuroendocrinol. 29:1 (2008), 88–113.
7. [7] Gibbs, R.B., Estrogen therapy and cognition: a review of the cholinergic hypothesis. Endocr. Rev. 31:2 (2010), 224–253.
8. [8] Zandi, P.P., et al. Hormone replacement therapy and incidence of Alzheimer disease in older women: the Cache County Study. JAMA 288:17 (2002), 2123–2129.
9. [9] Bagger, Y.Z., et al. Early postmenopausal hormone therapy may prevent cognitive impairment later in life. Menopause 12:1 (2005), 12–17.
10. [10] De Nicola, A.F., et al. Neuroprotection and sex steroid hormones: evidence of estradiol-Mediated protection in hypertensive encephalopathy. Mini Rev. Med. Chem., 12(11), 2012.
11. [11] Saldanha, C.J., Duncan, K.A., Walters, B.J., Neuroprotective actions of brain aromatase. Front. Neuroendocrinol. 30:2 (2009), 106–118.
12. [12] Biegon, A., Alia-Klein, N., Fowler, J.S., Potential contribution of aromatase inhibition to the effects of nicotine and related compounds on the brain. Front. Pharmacol., 3, 2012, 185.
13. [13] Balthazart, J., et al. Behavioral effects of brain-derived estrogens in birds. Ann. N. Y. Acad. Sci. 1163 (2009), 31–48.
14. [14] Putnam, S.K., Sato, S., Hull, E.M., Effects of testosterone metabolites on copulation and medial preoptic dopamine release in castrated male rats. Horm. Behav. 44:5 (2003), 419–426.
15. [15] Putnam, S.K., et al. Effects of testosterone metabolites on copulation: medial preoptic dopamine, and NOS-immunoreactivity in castrated male rats. Horm. Behav. 47:5 (2005), 513–522.
16. [16] Carroll, R.S., Weaver, C.E., Baum, M.J., Evidence implicating aromatization of testosterone in the regulation of male ferret sexual behavior. Physiol. Behav. 42:5 (1988), 457–460.
17. [17] Konkle, A.T., McCarthy, M.M., Developmental time course of estradiol, testosterone, and dihydrotestosterone levels in discrete regions of male and female rat brain. Endocrinology 152:1 (2011), 223–235.
18. [18] Soma, K.K., et al. Brain aromatase, 5 alpha-reductase, and 5 beta-reductase change seasonally in wild male song sparrows: relationship to aggressive and sexual behavior. J. Neurobiol. 56:3 (2003), 209–221.
19. [19] Wacker, D.W., et al. Seasonal changes in aromatase and androgen receptor: but not estrogen receptor mRNA expression in the brain of the free-living male song sparrow, Melospiza melodia morphna. J. Comp. Neurol. 518:18 (2010), 3819–3835.
20. [20] Fusani, L., et al. Seasonal expression of androgen receptors: estrogen receptors, and aromatase in the canary brain in relation to circulating androgens and estrogens. J. Neurobiol. 43:3 (2000), 254–268.
21. [21] Bakker, J., Brock, O., Early oestrogens in shaping reproductive networks: evidence for a potential organisational role of oestradiol in female brain development. J. Neuroendocrinol. 22:7 (2010), 728–735.
22. [22] Carani, C., et al. Role of oestrogen in male sexual behaviour: insights from the natural model of aromatase deficiency. Clin. Endocrinol. (Oxf.) 51:4 (1999), 517–524.
23. [23] Roselli, C.E., Klosterman, S.A., Sexual differentiation of aromatase activity in the rat brain: effects of perinatal steroid exposure. Endocrinology 139:7 (1998), 3193–3201.
24. [24] Roselli, C.E., Resko, J.A., Aromatase activity in the rat brain: hormonal regulation and sex differences. J. Steroid Biochem. Mol. Biol. 44:4-6 (1993), 499–508.
25. [25] Peterson, R.S., et al. Aromatase expression and cell proliferation following injury of the adult zebra finch hippocampus. Dev. Neurobiol. 67:14 (2007), 1867–1878.
26. [26] Carswell, H.V., et al. Brain aromatase expression after experimental stroke: topography and time course. J. Steroid Biochem. Mol. Biol. 96:1 (2005), 89–91.
27. [27] Pietranera, L., et al. Increased aromatase expression in the hippocampus of spontaneously hypertensive rats: effects of estradiol administration. Neuroscience 174 (2011), 151–159.
28. 28 Duncan, K.A., Saldanha, C.J., Neuroinflammation induces glial aromatase expression in the uninjured songbird brain. J. Neuroinflammation, 8, 2011, 81.
29. [29] Roselli, C.E., et al. Anatomic distribution and regulation of aromatase gene expression in the rat brain. Biol. Reprod. 58:1 (1998), 79–87.
30. [30] Roselli, C., Resko, J., Greenstein, B., (eds.) In Vitro Assay of Aromatase Activity in the Central Nervous System, in Neuroendocrine Research Methods, 1991, Harwood, Chur, Switzerland, 937–951.
31. [31] Sierra, A., Azcoitia, I., Garcia-Segura, L., Endogenous estrogen formation is neuroprotective in model of cerebellar ataxia. Endocrine 21:1 (2003), 43–51.
32. [32] Takahashi, K., et al. Imaging of aromatase distribution in rat and rhesus monkey brains with [11C]vorozole. Nucl. Med. Biol. 33:5 (2006), 599–605.
33. [33] Tabatadze, N., Sato, S.M., Woolley, C.S., Quantitative analysis of long-form aromatase mRNA in the male and female rat brain. PLoS One, 9(7), 2014, e100628.
34. [34] Roselli, C.E., Abdelgadir, S.E., Resko, J.A., Regulation of aromatase gene expression in the adult rat brain. Brain Res. Bull. 44:4 (1997), 351–357.
35. [35] Roselli, C.E., Ellinwood, W.E., Resko, J.A., Regulation of brain aromatase activity in rats. Endocrinology 114:1 (1984), 192–200.
36. [36] Nelson, R.E., et al. Liquid chromatography-tandem mass spectrometry assay for simultaneous measurement of estradiol and estrone in human plasma. Clin. Chem. 50:2 (2004), 373–384.
37. [37] Pfaffl, M.W., A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res., 29(9), 2001, e45.
38. [38] Bolcato, C.A., et al. Determination of 20-hydroxyeicosatetraenoic acid in microsomal incubates using high-performance liquid chromatography-mass spectrometry (HPLC-MS). J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 794:2 (2003), 363–372.
39. [39] Dean, S.L., et al. Prostaglandin E2 stimulates estradiol synthesis in the cerebellum postnatally with associated effects on Purkinje neuron dendritic arbor and electrophysiological properties. Endocrinology 153:11 (2012), 5415–5427.
40. [40] Peterson, R.S., et al. Aromatase is pre-synaptic and sexually dimorphic in the adult zebra finch brain. Proc. Biol. Sci. 272:1576 (2005), 2089–2096.
41. [41] Balthazart, J., Ball, G.F., Is brain estradiol a hormone or a neurotransmitter?. Trends Neurosci. 29:5 (2006), 241–249.