Single plasmid systems for inducible dual protein expression and for CRISPR-Cas9/CRISPRi gene regulation in lactic acid bacterium Lactococcus lactis

Scientific Reports

Volumn 8, Issue 1, 2018, Pages

  Berlec, Aleš ^a   Škrlec, Katja ^a,b   Kocjan, Janja ^a,b   Olenic, Maria ^a,c   Štrukelj, Borut ^a,b  

^a JO EF STEFAN INSTITUTE   (Slovenia)

^b UNIVERSITY OF LJUBLJANA   (Slovenia)

^c Charles University in Prague Faculty of Pharmacy   (Czech Republic)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIINFECTIVE AGENT; DARPIN E2 79; FUSION PROTEIN; GUIDE RNA; HEAT SHOCK PROTEIN; IMMUNOGLOBULIN G; METHYLTRANSFERASE; NISIN;

BACTERIAL GENOME; CHEMISTRY; CRISPR CAS SYSTEM; DRUG EFFECT; FERMENTATION; GENE EDITING; GENE EXPRESSION REGULATION; GENETIC ENGINEERING; GENETICS; HUMAN; LACTOCOCCUS LACTIS; METABOLISM; MOLECULAR CLONING; PLASMID; PROCEDURES; PROMOTER REGION; TRANSGENE;

ANTI-BACTERIAL AGENTS; CLONING, MOLECULAR; CRISPR-CAS SYSTEMS; FERMENTATION; GENE EDITING; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC ENGINEERING; GENOME, BACTERIAL; HEAT-SHOCK PROTEINS; HUMANS; IMMUNOGLOBULIN G; LACTOCOCCUS LACTIS; METHYLTRANSFERASES; NISIN; PLASMIDS; PROMOTER REGIONS, GENETIC; RECOMBINANT FUSION PROTEINS; RNA, GUIDE; TRANSGENES;

EID: 85040775746     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/s41598-018-19402-1     Document Type: Article

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