Cre/loxP-Mediated Multicopy Integration of the Mevalonate Operon into the Genome of Methylobacterium extorquens AM1

Applied Biochemistry and Biotechnology

Volumn 185, Issue 3, 2018, Pages 565-577

  Liang, Wei Fan ^a,b   Sun, Ming Yang ^a   Cui, Lan Yu ^a   Zhang, Chong ^a   Xing, Xin Hui ^a  

^a TSINGHUA UNIVERSITY   (China)

^b Guangdong Hainabiotech CO   (China)

Author keywords

Cre loxP; Methylobacterium extorquens AM1; Mevalonate; Multicopy; mvt3 operon

Indexed keywords

BIOCHEMISTRY;

CRE-LOXP; EXTORQUENS AM1; MEVALONATE; MULTI COPIES; MVT3 OPERON;

GENES;

CRE RECOMBINASE; MEVALONIC ACID; ACETYL COENZYME A ACETYLTRANSFERASE; HYDROXYMETHYLGLUTARYL COENZYME A REDUCTASE; HYDROXYMETHYLGLUTARYL COENZYME A SYNTHASE; INTEGRASE; KANAMYCIN;

ARTICLE; BACTERIAL GENOME; CELABC GENE; CONTROLLED STUDY; DNA INTEGRATION; ELECTROPORATION; ESCHERICHIA COLI; GENE; GENE KNOCKOUT; HOMOLOGOUS RECOMBINATION; LIMIT OF QUANTITATION; LOXP SITE; METHYLOBACTERIUM EXTORQUENS; MUTANT; NONHUMAN; OPERON; PLASMID; PROMOTER REGION; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; DRUG EFFECT; GENE DOSAGE; GENETIC RECOMBINATION; GENETICS; METABOLIC ENGINEERING; METABOLISM; MICROBIAL SENSITIVITY TEST; MUTATION; PROOF OF CONCEPT;

ACETYL-COA C-ACETYLTRANSFERASE; GENE DOSAGE; GENOME, BACTERIAL; HYDROXYMETHYLGLUTARYL COA REDUCTASES; HYDROXYMETHYLGLUTARYL-COA SYNTHASE; INTEGRASES; KANAMYCIN; METABOLIC ENGINEERING; METHYLOBACTERIUM EXTORQUENS; MEVALONIC ACID; MICROBIAL SENSITIVITY TESTS; MUTATION; OPERON; PROOF OF CONCEPT STUDY; RECOMBINATION, GENETIC;

EID: 85038073461     PISSN: 02732289     EISSN: 15590291     Source Type: Journal    
DOI: 10.1007/s12010-017-2673-3     Document Type: Article

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