Metabolic engineering of Escherichia coli for producing astaxanthin as the predominant carotenoid

Marine Drugs

Volumn 15, Issue 10, 2017, Pages

  Lu, Qian ^a   Bu, Yi Fan ^a   Liu, Jian Zhong ^a  

^a SUN YAT SEN UNIVERSITY   (China)

Author keywords

Astaxanthin; Escherichia coli; Metabolic engineering; carotene hydroxylase; carotene ketolase

Indexed keywords

ASTAXANTHIN; BETA CAROTENE KETOLASE; OXYGENASE; TRANSFERASE; UNCLASSIFIED DRUG; ZEAXANTHIN; BETA-CAROTENE KETOLASE; CAROTENOID; MIXED FUNCTION OXIDASE; XANTHOPHYLL;

ARTICLE; BACTERIAL CELL; BACTERIAL GENE; BREVUNDIMONAS; CHLAMYDOMONAS REINHARDTII; CONTROLLED STUDY; CRTZ GENE; ENZYME ACTIVITY; ESCHERICHIA COLI; METABOLIC ENGINEERING; NONHUMAN; PANTOEA; PANTOEA ANANATIS; PARACOCCUS; SPHINGOMONAS; METABOLISM; PLASMID; PROCEDURES;

CAROTENOIDS; ESCHERICHIA COLI; METABOLIC ENGINEERING; MIXED FUNCTION OXYGENASES; OXYGENASES; PLASMIDS; XANTHOPHYLLS; ZEAXANTHINS;

EID: 85032825588     PISSN: None     EISSN: 16603397     Source Type: Journal    
DOI: 10.3390/md15100296     Document Type: Article

References (25)

1. Hussein, G.; Sankawa, U.; Goto, H.; Matsumoto, K.; Watanabe, H. Astaxanthin, a carotenoid with potential in human health and nutrition. J. Nat. Prod. 2006, 69, 443–449. [CrossRef] [PubMed]
2. Lee, P.C.; Schmidt-Dannert, C. Metabolic engineering towards biotechnological production of carotenoids in microorganisms. Appl. Microbiol. Biotechnol. 2002, 60, 1–11. [PubMed]
3. Ye, V.M.; Bhatia, S.K. Pathway engineering strategies for production of beneficial carotenoids in microbial hosts. Biotechnol. Lett. 2012, 34, 1405–1414. [CrossRef] [PubMed]
4. Lemuth, K.; Steuer, K.; Albermann, C. Engineering of a plasmid-free Escherichia coli strain for improved in vivo biosynthesis of astaxanthin. Microb. Cell Fact. 2011, 10, 29. [CrossRef] [PubMed]
5. Zhou, P.P.; Ye, L.D.; Xie, W.P.; Lv, X.M.; Yu, H.W. Highly efficient biosynthesis of astaxanthin in Saccharomyces cerevisiae by integration and tuning of algal crtZ and bkt. Appl. Microbiol. Biotechnol. 2015, 99, 8419–8428. [CrossRef] [PubMed]
6. Zhou, P.P.; Xie, W.P.; Li, A.P.; Wang, F.; Yao, Z.; Bian, Q.; Zhu, Y.Q.; Yu, H.W.; Ye, L.D. Alleviation of metabolic bottleneck by combinatorial engineering enhanced astaxanthin synthesis in Saccharomyces cerevisiae. Enzyme Microb. Technol. 2017, 100, 28–36. [CrossRef] [PubMed]
7. Henke, N.A.; Heider, S.A.E.; Peters-Wendisch, P.; Wendisch, V.F. Production of the marine carotenoid astaxanthin by metabolically engineered Corynebacterium glutamicum. Mar. Drugs 2016, 14, 124. [CrossRef] [PubMed]
8. Tao, L.A.; Wilczek, J.; Odom, J.M.; Cheng, Q.O. Engineering a β-carotene ketolase for astaxanthin production. Metab. Eng. 2006, 8, 523–531. [CrossRef] [PubMed]
9. Martin, J.F.; Gudina, E.; Barredo, J.L. Conversion of β-carotene into astaxanthin: Two separate enzymes or a bifunctional hydroxylase-ketolase protein? Microb. Cell Fact. 2008, 7, 3. [CrossRef] [PubMed]
10. Misawa, N. Carotenoid beta-ring hydroxylase and ketolase from marine bacteria-promiscuous enzymes for synthesizing functional xanthophylls. Mar. Drugs 2011, 9, 757–771. [CrossRef] [PubMed]
11. Choi, S.K.; Nishida, Y.; Matsuda, S.; Adachi, K.; Kasai, H.; Peng, X.; Komemushi, S.; Miki, W.; Misawa, N. Characterization of beta-carotene ketolases, CrtW, from marine bacteria by complementation analysis in Escherichia coli. Mar. Biotechnol. 2005, 7, 515–522. [CrossRef] [PubMed]
12. Choi, S.K.; Harada, H.; Matsuda, S.; Misawa, N. Characterization of two beta-carotene ketolases, CrtO and CrtW, by complementation analysis in Escherichia coli. Appl. Microbiol. Biotechnol. 2007, 75, 1335–1341. [CrossRef] [PubMed]
13. Makino, T.; Harada, H.; Ikenaga, H.; Matsuda, S.; Takaichi, S.; Shindo, K.; Sandmann, G.; Ogata, T.; Misawa, N. Characterization of cyanobacterial carotenoid ketolase CrtW and hydroxylase CrtR by complementation analysis in Escherichia coli. Plant Cell Physiol. 2008, 49, 1867–1878. [CrossRef] [PubMed]
14. Scaife, M.A.; Burja, A.M.; Wright, P.C. Characterization of cyanobacterial β-carotene ketolase and hydroxylase Genes in Escherichia coli, and their application for astaxanthin biosynthesis. Biotechnol. Bioeng. 2009, 103, 944–955. [CrossRef] [PubMed]
15. Scaife, M.A.; Ma, C.A.; Ninlayarn, T.; Wright, P.C.; Armenta, R.E. Comparative analysis of beta-carotene hydroxylase genes for astaxanthin biosynthesis. J. Nat. Prod. 2012, 75, 1117–1124. [CrossRef] [PubMed]
16. Ye, R.W.; Stead, K.J.; Yao, H.; He, H.X. Mutational and functional analysis of the beta-carotene ketolase involved in the production of canthaxanthin and astaxanthin. Appl. Environ. Microb. 2006, 72, 5829–5837. [CrossRef] [PubMed]
17. Zhong, Y.J.; Huang, J.C.; Liu, J.; Li, Y.; Jiang, Y.; Xu, Z.F.; Sandmann, G.; Chen, F. Functional characterization of various algal carotenoid ketolases reveals that ketolating zeaxanthin efficiently is essential for high production of astaxanthin in transgenic Arabidopsis. J. Exp. Bot. 2011, 62, 3659–3669. [CrossRef] [PubMed]
18. Li, X.R.; Tian, G.Q.; Shen, H.J.; Liu, J.Z. Metabolic engineering of Escherichia coli to produce zeaxanthin. J. Ind. Microbiol. Biotechnol. 2015, 42, 627–636. [CrossRef] [PubMed]
19. Shen, H.J.; Cheng, B.Y.; Zhang, Y.M.; Tang, L.; Li, Z.; Bu, Y.F.; Li, X.R.; Tian, G.Q.; Liu, J.Z. Dynamic control of the mevalonate pathway expression for improved zeaxanthin production in Escherichia coli and comparative proteome analysis. Metab. Eng. 2016, 38, 180–190. [CrossRef] [PubMed]
20. Fraser, P.D.; Miura, Y.; Misawa, N. In vitro characterization of astaxanthin biosynthetic enzymes. J. Biol. Chem. 1997, 272, 6128–6135. [CrossRef] [PubMed]
21. Fraser, P.D.; Shimada, H.; Misawa, N. Enzymic confirmation of reactions involved in routes to astaxanthin formation, elucidated using a direct substrate in vitro assay. Eur. J. Biochem. 1998, 252, 229–236. [CrossRef] [PubMed]
22. Ma, T.; Zhou, Y.J.; Li, X.W.; Zhu, F.Y.; Cheng, Y.B.; Liu, Y.; Deng, Z.X.; Liu, T.G. Genome mining of astaxanthin biosynthetic genes from Sphingomonas sp. ATCC 55669 for heterologous overproduction in Escherichia coli. Biotechnol. J. 2016, 11, 228–237. [CrossRef] [PubMed]
23. Zelcbuch, L.; Antonovsky, N.; Bar-Even, A.; Levin-Karp, A.; Barenholz, U.; Dayagi, M.; Liebermeister, W.; Flamholz, A.; Noor, E.; Amram, S.; et al. Spanning high-dimensional expression space using ribosome-binding site combinatorics. Nucleic Acids Res. 2013, 41. [CrossRef] [PubMed]
24. Guzman, L.M.; Belin, D.; Carson, M.J.; Beckwith, J. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J. Bacteriol. 1995, 177, 4121–4130. [CrossRef] [PubMed]
25. Boel, G.; Letso, R.; Neely, H.; Price, W.N.; Wong, K.H.; Su, M.; Luff, J.D.; Valecha, M.; Everett, J.K.; Acton, T.B.; et al. Codon influence on protein expression in E. coli correlates with mRNA levels. Nature 2016, 529, 358–363. [CrossRef] [PubMed]