Spontaneous pepsin C-catalyzed activation of human pepsinogen C in transgenic rice cell suspension culture: Production and characterization of human pepsin C

Enzyme and Microbial Technology

Volumn 108, Issue , 2018, Pages 66-73

  Islam, Md Reyazul ^a   Kim, Nan Sun ^a   Jung, Jae Wan ^a   Kim, Hyo Boon ^a   Han, So Chon ^a   Yang, Moon Sik ^a  

^a Chonbuk National University   (South Korea)

Author keywords

Human pepsin C; Human pepsinogen C (hPGC); Rice cell suspension culture; Rice amylase 3D promoter

Indexed keywords

AMYLASES; BACTERIA; CELL CULTURE; CLONING; ELECTROPHORESIS; GENE EXPRESSION; POLYMERASE CHAIN REACTION; PURIFICATION;

AGROBACTERIUM TUMEFACIENS; HUMAN PEPSIN; HUMAN PEPSINOGEN C (HPGC); NORTHERN BLOT ANALYSIS; PLANT EXPRESSION VECTORS; RICE CELLS; STARVATION CONDITIONS; WESTERN-BLOT ANALYSIS;

PLANTS (BOTANY);

AGAROSE; AMYLASE; GASTRICSIN; GENOMIC DNA; HEMOGLOBIN; NITRILOTRIACETATE NICKEL; PEPSINOGEN II; PLANT DNA; PLANT PROTEIN; PLANT RNA; POLYHISTIDINE TAG; RICE ALPHA AMYLASE 3D; SIGNAL PEPTIDE; SUGAR; SYNTHETIC DNA; UNCLASSIFIED DRUG; PEPSIN A; RECOMBINANT PROTEIN;

AGROBACTERIUM TUMEFACIENS; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CELL SUSPENSION; CODON USAGE; CULTURE MEDIUM; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME MECHANISM; ENZYME PURIFICATION; ENZYME SYNTHESIS; EXPRESSION VECTOR; GENE AMPLIFICATION; GENE EXPRESSION; HUMAN; MOLECULAR WEIGHT; NONHUMAN; NORTHERN BLOTTING; PH; PLANT CELL; PLANT CELL CULTURE; PLANT CHROMOSOME; PLANT GENE; POLYMERASE CHAIN REACTION; PROCESS OPTIMIZATION; PROMOTER REGION; PROTEIN HYDROLYSIS; QUANTITATIVE ASSAY; RICE; SEQUENCE ANALYSIS; STARVATION; SUSPENSION CELL CULTURE; TEMPERATURE; TRANSGENIC PLANT; WESTERN BLOTTING; ANIMAL; BOVINE; CELL LINE; CHEMISTRY; ENZYME SPECIFICITY; GENE VECTOR; GENETICS; KINETICS; METABOLISM; ORYZA;

AGROBACTERIUM TUMEFACIENS; AMINO ACID SEQUENCE; ANIMALS; CATTLE; CELL LINE; ENZYME ACTIVATION; GENETIC VECTORS; HEMOGLOBINS; HUMANS; HYDROGEN-ION CONCENTRATION; KINETICS; ORYZA; PEPSIN A; PEPSINOGEN C; PLANTS, GENETICALLY MODIFIED; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY; TEMPERATURE;

EID: 85029715700     PISSN: 01410229     EISSN: 18790909     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2017.09.006     Document Type: Article

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