Probiotic reduces bacterial translocation in type 2 diabetes mellitus: A randomised controlled study

Scientific Reports

Volumn 7, Issue 1, 2017, Pages

  Sato, Junko ^a   Kanazawa, Akio ^a   Azuma, Kosuke ^a   Ikeda, Fuki ^a   Goto, Hiromasa ^a   Komiya, Koji ^a   Kanno, Rei ^a   Tamura, Yoshifumi ^a   Asahara, Takashi ^a,b   Takahashi, Takuya ^a,b   Nomoto, Koji ^a,b   Yamashiro, Yuichiro ^a   Watada, Hirotaka ^a  

^a JUNTENDO UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^b YAKULT CENTRAL INSTITUTE FOR MICROBIOLOGICAL RESEARCH   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

PROBIOTIC AGENT;

AGED; ANIMAL; BACTERIAL COUNT; BLOOD; CLOSTRIDIUM; CONTROLLED STUDY; FECES; FEMALE; FERMENTATION; HUMAN; INTESTINE FLORA; ISOLATION AND PURIFICATION; JAPAN; LACTOBACILLUS CASEI; MALE; MICROBIOLOGY; MICROFLORA; MIDDLE AGED; MILK; NON INSULIN DEPENDENT DIABETES MELLITUS; PHYSIOLOGY; RANDOMIZED CONTROLLED TRIAL;

AGED; ANIMALS; CLOSTRIDIUM; COLONY COUNT, MICROBIAL; DIABETES MELLITUS, TYPE 2; FECES; FEMALE; FERMENTATION; GASTROINTESTINAL MICROBIOME; HUMANS; JAPAN; LACTOBACILLUS CASEI; MALE; MICROBIOTA; MIDDLE AGED; MILK; PROBIOTICS;

EID: 85029706141     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/s41598-017-12535-9     Document Type: Article

References (40)

1. Worldwide trends in diabetes since 1980: a pooled analysis of 751 population-based studies with 4.4 million participants. Lancet (London, England) 387, 1513-1530, https://doi.org/10.1016/s0140-6736(16)00618-8 (2016).
2. Mazidi, M., Pennathur, S. & Afshinnia, F. Link of dietary patterns with metabolic syndrome: analysis of the National Health and Nutrition Examination Survey. Nutr & Diabetes 7, e255, https://doi.org/10.1038/nutd.2017.11 (2017).
3. Tolhurst, G. et al. Short-chain fatty acids stimulate glucagon-like peptide-1 secretion via the G-protein-coupled receptor FFAR2. Diabetes 61, 364-371, https://doi.org/10.2337/db11-1019 (2012).
4. De Vadder, F. et al. Microbiota-generated metabolites promote metabolic benefits via gut-brain neural circuits. Cell 156, 84-96, https://doi.org/10.1016/j.cell.2013.12.016 (2014).
5. Kimura, I. et al. The gut microbiota suppresses insulin-mediated fat accumulation via the short-chain fatty acid receptor GPR43. Nat Commun 4, 1829, https://doi.org/10.1038/ncomms2852 (2013).
6. Perry, R. J. et al. Acetate mediates a microbiome-brain-beta-cell axis to promote metabolic syndrome. Nature 534, 213-217, https:// doi.org/10.1038/nature18309 (2016).
7. Cani, P. D. et al. Changes in gut microbiota control metabolic endotoxemia-induced inflammation in high-fat diet-induced obesity and diabetes in mice. Diabetes 57, 1470-1481, https://doi.org/10.2337/db07-1403 (2008).
8. Cani, P. D. et al. Metabolic endotoxemia initiates obesity and insulin resistance. Diabetes 56, 1761-1772, https://doi.org/10.2337/ db06-1491 (2007).
9. Naito, E. et al. Beneficial effect of oral administration of Lactobacillus casei strain Shirota on insulin resistance in diet-induced obesity mice. J of Applied Microbiol 110, 650-657, https://doi.org/10.1111/j.1365-2672.2010.04922.x (2011).
10. Amar, J. et al. Involvement of tissue bacteria in the onset of diabetes in humans: evidence for a concept. Diabetologia 54, 3055-3061, https://doi.org/10.1007/s00125-011-2329-8 (2011).
11. Sato, J. et al. Gut dysbiosis and detection of live gut bacteria in blood of Japanese patients with type 2 diabetes. Diabetes Care 37, 2343-2350, https://doi.org/10.2337/dc13-2817 (2014).
12. Matsumoto, K. et al. Effects of a probiotic fermented milk beverage containing Lactobacillus casei strain Shirota on defecation frequency, intestinal microbiota, and the intestinal environment of healthy individuals with soft stools. J Biosci Bioeng 110, 547-552, https://doi.org/10.1016/j.jbiosc.2010.05.016 (2010).
13. Nagata, S. et al. Effect of the continuous intake of probiotic-fermented milk containing Lactobacillus casei strain Shirota on fever in a mass outbreak of norovirus gastroenteritis and the faecal microflora in a health service facility for the aged. Br J Nutr 106, 549-556, https://doi.org/10.1017/S000711451100064X (2011).
14. Matsuzaki, T., Yamazaki, R., Hashimoto, S. & Yokokura, T. Antidiabetic effects of an oral administration of Lactobacillus casei in a non-insulin-dependent diabetes mellitus (NIDDM) model using KK-Ay mice. Endocr J 44, 357-365 (1997).
15. Miao, W. et al. Sodium Butyrate Promotes Reassembly of Tight Junctions in Caco-2 Monolayers Involving Inhibition of MLCK/ MLC2 Pathway and Phosphorylation of PKCbeta2. Int J of Mol Sci 17, doi:https://doi.org/10.3390/ijms17101696 (2016).
16. Hsieh, C. Y. et al. Strengthening of the intestinal epithelial tight junction by Bifidobacterium bifidum. Physiol rep 3, https://doi. org/10.14814/phy2.12327 (2015).
17. Tong, L. C. et al. Propionate Ameliorates Dextran Sodium Sulfate-Induced Colitis by Improving Intestinal Barrier Function and Reducing Inflammation and Oxidative Stress. Front Pharmacol 7, 253, https://doi.org/10.3389/fphar.2016.00253 (2016).
18. Stadlbauer, V. et al. Lactobacillus casei Shirota Supplementation Does Not Restore Gut Microbiota Composition and Gut Barrier in Metabolic Syndrome: A Randomized Pilot Study. PloS One 10, e0141399, https://doi.org/10.1371/journal.pone.0141399 (2015).
19. Leber, B. et al. The influence of probiotic supplementation on gut permeability in patients with metabolic syndrome: an open label, randomized pilot study. Eur J Clin Nutr 66, 1110-1115, https://doi.org/10.1038/ejcn.2012.103 (2012).
20. Hulston, C. J., Churnside, A. A. & Venables, M. C. Probiotic supplementation prevents high-fat, overfeeding-induced insulin resistance in human subjects. Br J Nutr 113, 596-602, https://doi.org/10.1017/S0007114514004097 (2015).
21. Mobini, R. et al. Metabolic effects of Lactobacillus reuteri DSM 17938 in Patients with Type 2 Diabetes: A Randomized Controlled Trial. Diabetes Obes Metab, https://doi.org/10.1111/dom.12861 (2016).
22. Asemi, Z., Zare, Z., Shakeri, H., Sabihi, S. S. & Esmaillzadeh, A. Effect of multispecies probiotic supplements on metabolic profiles, hs-CRP, and oxidative stress in patients with type 2 diabetes. Ann Nutr Metab 63, 1-9, https://doi.org/10.1159/000349922 (2013).
23. Amar, J. et al. Intestinal mucosal adherence and translocation of commensal bacteria at the early onset of type 2 diabetes: molecular mechanisms and probiotic treatment. EMBO Mol Med 3, 559-572, https://doi.org/10.1002/emmm.201100159 (2011).
24. Matsuda, K. et al. Establishment of an analytical system for the human fecal microbiota, based on reverse transcription-quantitative PCR targeting of multicopy rRNA molecules. Appl Environ Microbiol 75, 1961-1969, https://doi.org/10.1128/AEM.01843-08 (2009).
25. Sakaguchi, S. et al. Bacterial rRNA-targeted reverse transcription-PCR used to identify pathogens responsible for fever with neutropenia. J Clin Microbiol 48, 1624-1628, https://doi.org/10.1128/JCM.01724-09 (2010).
26. Yokoyama, Y. et al. Effect of Perioperative Synbiotic Treatment on Bacterial Translocation and Postoperative Infectious Complications after Pancreatoduodenectomy. Dig Surg 33, 220-229, https://doi.org/10.1159/000444459 (2016).
27. Matsuki, T., Watanabe, K., Fujimoto, J., Takada, T. & Tanaka, R. Use of 16S rRNA gene-targeted group-specific primers for real-time PCR analysis of predominant bacteria in human feces. Appl Environ Microbiol 70, 7220-7228, https://doi.org/10.1128/ AEM.70.12.7220-7228.2004 (2004).
28. Hayashi, H., Sakamoto, M., Kitahara, M. & Benno, Y. Diversity of the Clostridium coccoides group in human fecal microbiota as determined by 16S rRNA gene library. FEMS Microbiol Lett 257, 202-207, https://doi.org/10.1111/j.1574-6968.2006.00171.x (2006).
29. Okubo, H. et al. Lactobacillus casei strain Shirota protects against nonalcoholic steatohepatitis development in a rodent model. Am J of Physiol Gastrointest and Liver Physiol 305, G911-918, https://doi.org/10.1152/ajpgi.00225.2013 (2013).
30. Ahrne, S. & Hagslatt, M. L. Effect of lactobacilli on paracellular permeability in the gut. Nutrients 3, 104-117, https://doi. org/10.3390/nu3010104 (2011).
31. Ahl, D. et al. Lactobacillus reuteri increases mucus thickness and ameliorates dextran sulphate sodium-induced colitis in mice. Acta physiologica (Oxford, England) 217, 300-310, https://doi.org/10.1111/apha.12695 (2016).
32. Yang, F. et al. Lactobacillus reuteri I5007 modulates tight junction protein expression in IPEC-J2 cells with LPS stimulation and in newborn piglets under normal conditions. BMC microbiology 15, 32, https://doi.org/10.1186/s12866-015-0372-1 (2015).
33. Di Luccia, B. et al. Lactobacillus gasseri SF1183 affects intestinal epithelial cell survival and growth. PloS One 8, e69102, https://doi. org/10.1371/journal.pone.0069102 (2013).
34. Wang, C. et al. Intestinal Microbiota Profiles of Healthy Pre-School and School-Age Children and Effects of Probiotic Supplementation. Ann Nutr Metab 67, 257-266, https://doi.org/10.1159/000441066 (2015).
35. Nagata, S. et al. The Effectiveness of Lactobacillus Beverages in Controlling Infections among the Residents of an Aged Care Facility: A Randomized Placebo-Controlled Double-Blind Trial. Ann Nutr Metab 68, 51-59, https://doi.org/10.1159/000442305 (2016).
36. Tripolt, N. J. et al. Short communication: Effect of supplementation with Lactobacillus casei Shirota on insulin sensitivity, beta-cell function, and markers of endothelial function and inflammation in subjects with metabolic syndrome-a pilot study. J Dairy Sci 96, 89-95, https://doi.org/10.3168/jds.2012-5863 (2013).
37. Matsuda, K., Tsuji, H., Asahara, T., Kado, Y. & Nomoto, K. Sensitive quantitative detection of commensal bacteria by rRNA-targeted reverse transcription-PCR. Appl Environ Microbiol 73, 32-39, https://doi.org/10.1128/AEM.01224-06 (2007).
38. Ohigashi, S. et al. Changes of the intestinal microbiota, short chain fatty acids, and fecal pH in patients with colorectal cancer. Dig Dis Sci 58, 1717-1726, https://doi.org/10.1007/s10620-012-2526-4 (2013).
39. Fujimoto, J., Tanigawa, K., Kudo, Y., Makino, H. & Watanabe, K. Identification and quantification of viable Bifidobacterium breve strain Yakult in human faeces by using strain-specific primers and propidium monoazide. J Appl Microbiol 110, 209-217, https://doi. org/10.1111/j.1365-2672.2010.04873.x (2011).
40. Fujimoto, J., Matsuki, T., Sasamoto, M., Tomii, Y. & Watanabe, K. Identification and quantification of Lactobacillus casei strain Shirota in human feces with strain-specific primers derived from randomly amplified polymorphic DNA. Int J Food Microbiol 126, 210-215, https://doi.org/10.1016/j.ijfoodmicro.2008.05.022 (2008).