Use of CRISPR-modified human stem cell organoids to study the origin of mutational signatures in cancer

Science

Volumn 358, Issue 6360, 2017, Pages 234-238

  Drost, Jarno ^a,g   Van Boxtel, Ruben ^a,g   Blokzijl, Francis ^a   Mizutani, Tomohiro ^a   Sasaki, Nobuo ^a   Sasselli, Valentina ^a   De Ligt, Joep ^a   Behjati, Sam ^b,c   Grolleman, Judith E ^d   Van Wezel, Tom ^e   Nik Zainal, Serena ^b,f   Kuiper, Roland P ^d,g   Cuppen, Edwin ^a   Clevers, Hans ^a,g  

^a UNIVERSITY MEDICAL CENTER UTRECHT   (Netherlands)

^b WELLCOME TRUST SANGER INSTITUTE   (United Kingdom)

^c UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^d RADBOUD UNIVERSITY MEDICAL CENTER   (Netherlands)

^e LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

^f CAMBRIDGE UNIVERSITY HOSPITALS NHS FOUNDATION TRUST   (United Kingdom)

^g the Netherlands The Innovative Therapies for Children with Cancer Consortium   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

MUTL PROTEIN HOMOLOG 1; PUROMYCIN; DEOXYRIBONUCLEASE (PYRIMIDINE DIMER); NTHL1 PROTEIN, HUMAN;

CANCER; CELLS AND CELL COMPONENTS; DNA; ETIOLOGY; GENE; GENETIC ANALYSIS; GENOME; MUTATION; PROTEIN;

ARTICLE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; COLORECTAL CANCER; CONTROLLED STUDY; CYTOPLASM; DNA REPAIR; DNA REPLICATION; GENE DELETION; GENE EDITING; GENE EXPRESSION; GENE INACTIVATION; GENE MUTATION; GENE TARGETING; GENETIC PREDISPOSITION; GERMLINE MUTATION; HETEROZYGOSITY LOSS; HUMAN; HUMAN CELL; IN VITRO STUDY; INDEL MUTATION; LOSS OF FUNCTION MUTATION; MALIGNANT NEOPLASM; MICROSATELLITE INSTABILITY; MISMATCH REPAIR; MUTATION ACCUMULATION; MUTATION RATE; MUTATIONAL LOAD; NONSENSE MUTATION; NUCLEIC ACID BASE SUBSTITUTION; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SOMATIC MUTATION; STEM CELL; WHOLE GENOME SEQUENCING; BREAST TUMOR; COLON; COLORECTAL TUMOR; CRISPR CAS SYSTEM; FEMALE; GENETICS; MUTAGENESIS; NEOPLASM; ORGANOID;

BREAST NEOPLASMS; COLON; COLORECTAL NEOPLASMS; CRISPR-CAS SYSTEMS; DEOXYRIBONUCLEASE (PYRIMIDINE DIMER); DNA MISMATCH REPAIR; DNA REPAIR; DNA REPLICATION; FEMALE; GERM-LINE MUTATION; HUMANS; INDEL MUTATION; MUTAGENESIS; MUTL PROTEIN HOMOLOG 1; NEOPLASMS; ORGANOIDS; STEM CELLS;

EID: 85029574202     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.aao3130     Document Type: Article

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