Automatic dendritic spine quantification from confocal data with neurolucida 360

Current Protocols in Neuroscience

Volumn 2016, Issue , 2016, Pages 1.27.1-1.27.21

  Dickstein, Dara L ^a   Dickstein, Daniel R ^a   Janssen, William G M ^a   Hof, Patrick R ^a   Glaser, Jacob R ^b   Rodriguez, Alfredo ^b   O’Connor, Nate ^b   Angstman, Paul ^b   Tappan, Susan J ^b  

^a MOUNT SINAI SCHOOL OF MEDICINE   (United States)

^b MBF Bioscience   (United States)

Author keywords

Automated quantification; Confocal microscopy; Dendritic spines; Neurons

Indexed keywords

ARTICLE; AUTOMATION; CELL COUNT; CELL DENSITY; CELL FUNCTION; CELL STRUCTURE; CONFOCAL MICROSCOPY; CONTROLLED STUDY; CYTOMETRY; DENDRITIC SPINE; MORPHOMETRY; MOUSE; NERVE CELL; NERVE CELL PLASTICITY; NONHUMAN; PRIORITY JOURNAL; SOFTWARE; THREE DIMENSIONAL IMAGING; ANIMAL; DEVICES; ELECTRON MICROSCOPY; PATHOLOGY; PHYSIOLOGY; PROCEDURES;

ANIMALS; DENDRITIC SPINES; IMAGING, THREE-DIMENSIONAL; MICROSCOPY, CONFOCAL; MICROSCOPY, ELECTRON; NEURONAL PLASTICITY; NEURONS;

EID: 85029404244     PISSN: 19348584     EISSN: 19348576     Source Type: Journal    
DOI: 10.1002/cpns.16     Document Type: Article

References (54)

1. Benavides-Piccione, R., Ballesteros-Yanez, I., De-Felipe, J., and Yuste, R. 2002. Cortical area and species differences in dendritic spine morphology. J. Neurocytol. 31: 337-346. doi:10.1023/A:1024134312173.
2. Bloss, E.B., Janssen, W.G., Ohm, D.T., Yuk, F.J., Wadsworth, S., Saardi, K.M., McEwen, B.S., and Morrison, J.H. 2011. Evidence for reduced experience-dependent dendritic spine plasticity in the aging prefrontal cortex. J. Neurosci. 31: 7831-7839. doi: 10.1523/JNEU ROSCI.0839-11.2011.
3. Boyan, G. and Liu, Y. 2014. Dye coupling and immunostaining of astrocyte-like glia following intracellular injection of fluorochromes in brain slices of the grasshopper, Schistocerca gregaria. Methods Mol. Biol. 1082: 99-113. doi:10.1007/978-1-62703-655-9_7.
4. Brennan, A.R., Yuan, P., Dickstein, D.L., Rocher, A.B., Hof, P.R., Manji, H., and Arnsten, A.F. 2009. Protein kinase C activity is associated with prefrontal cortical decline in aging. Neurobiol. Aging 30: 782-792. doi:10.1016/j.neurobiolaging.2007.08.020.
5. Carlisle, H.J. and Kennedy, M.B. 2005. Spine architecture and synaptic plasticity. Trends Neurosci. 28:182-187. doi: 10.1016/j.tins.2005.01.008.
6. Dickstein, D.L., Weaver, C.M., Luebke, J.I., and Hof, P.R. 2013. Dendritic spine changes associated with normal aging. Neuroscience 251: 21-32. doi: 10.1016/j.neuroscience.2012.09.077.
7. Dickstein, D.L., Kabaso, D., Rocher, A.B., Luebke, J.I., Wearne, S.L., and Hof, P.R. 2007. Changes in the structural complexity of the aged brain. Aging Cell 6: 275-284. doi:10.1111/j.1474-9726.2007.00289.x.
8. Ding, J.B., Takasaki, K.T., and Sabatini, B.L. 2009. Supraresolution imaging in brain slices using stimulated-emission depletion two-photon laser scanning microscopy. Neuron 63: 429-437. doi:10.1016/j.neuron.2009.07.011.
9. Donohue, D.E. and Ascoli, G.A. 2011. Automated reconstruction of neuronal morphology: An overview. Brain Res. Rev. 67: 94-102. doi:10.1016/j.brainresrev.2010.11.003.
10. Dumitriu, D., Rodriguez, A., and Morrison, J.H. 2011. High-throughput, detailed, cell-specific neuroanatomy of dendritic spines usingmicroinjection and confocal microscopy. Nat. Protoc. 6:1391-1411. doi: 10.1038/nprot.2011.389.
11. Durand, C.M., Perroy, J., Loll, F., Perrais, D., Fagni, L., Bourgeron, T., Montcouquiol, M., and Sans, N. 2012. SHANK3 mutations identified in autism lead to modification of dendritic spine morphology via an actin-dependent mechanism. Mol. Psychiatry 17: 71-84. doi:10.1038/mp.2011.57.
12. Ethell, I.M. and Pasquale, E.B. 2005. Molecular mechanisms of dendritic spine development and remodeling. Prog. Neurobiol. 75: 161-205. doi:10.1016/j.pneurobio.2005.02.003.
13. Gibson, S.F. and Lanni, F. 1992. Experimental test of an analytical model of aberration in an oil-immersion objective lens used in threedimensional light microscopy. J. Opt. Soc. Am. A. 9: 154-166. doi: 10.1364/JOSAA.9.000154.
14. Golden, S.A., Christoffel, D.J., Heshmati, M., Hodes, G.E.,Magida, J.,Davis,K., Cahill, M.E., Dias, C., Ribeiro, E., Ables, J.L., Kennedy, P.J., Robison, A.J., Gonzalez-Maeso, J., Neve, R.L., Turecki, G., Ghose, S., Tamminga, C.A., and Russo, S.J. 2013. Epigenetic regulation of RAC1 induces synaptic remodeling in stress disorders and depression. Nat. Med. 19: 337-344. doi: 10.1038/nm.3090.
15. Hao, J., Rapp, P.R., Leffler, A.E., Leffler, S.R., Janssen, W.G., Lou, W., McKay, H., Roberts, J.A., Wearne, S.L., Hof, P.R., and Morrison, J.H. 2006. Estrogen alters spine number and morphology in prefrontal cortex of aged female rhesus monkeys. J. Neurosci. 26: 2571-2578. doi: 10.1523/JNEU ROSCI.3440-05.2006.
16. Hara, Y., Rapp, P.R., and Morrison, J.H. 2012. Neuronal and morphological bases of cognitive decline in aged rhesus monkeys. Age 34: 1051-1073. doi: 10.1007/s11357-011-9278-5.
17. Harris, K.M. and Stevens, J.K. 1989. Dendritic spines of CA 1 pyramidal cells in the rat hippocampus: Serial electron microscopy with reference to their biophysical characteristics. J. Neurosci. 9: 2982-2997.
18. Harris, K.M., Jensen, F.E., and Tsao, B. 1992. Three-dimensional structure of dendritic spines and synapses in rat hippocampus (CA1) at postnatal day 15 and adult ages: Implications for the maturation of synaptic physiology and long-term potentiation. J. Neurosci. 12: 2685-2705.
19. Hayashi-Takagi, A., Barker, P.B., and Sawa, A. 2011. Readdressing synaptic pruning theory for schizophrenia: Combination of brain imaging and cell biology. Commun. Integr. Biol. 4: 211-212. doi: 10.4161/cib.4.2.14492.
20. Holmes, T.J. 1992. Blind deconvolution of quantum-limited incoherent imagery: Maximum-likelihood approach. J. Opt. Soc. Am. A. 9: 1052-1061. doi: 10.1364/JOSAA.9.001052.
21. Hung, A.Y., Futai, K., Sala, C., Valtschanoff, J.G., Ryu, J., Woodworth, M.A., Kidd, F.L., Sung, C.C., Miyakawa, T., Bear, M.F., Weinberg, R.J., and Sheng, M. 2008. Smaller dendritic spines, weaker synaptic transmission, but enhanced spatial learning in mice lacking Shank1. J. Neurosci. 28: 1697-1708. doi: 10.1523/JNEU ROSCI.3032-07.2008.
22. Hutsler, J.J. and Zhang, H. 2010. Increased dendritic spine densities on cortical projection neurons in autism spectrum disorders. Brain Res. 1309:83-94. doi: 10.1016/j.brainres .2009.09.120.
23. Jones, E.G. and Powell, T.P. 1969. Morphological variations in the dendritic spines of the neocortex. J. Cell. Sci. 5: 509-529.
24. Kasai, H., Fukuda, M., Watanabe, S., Hayashi-Takagi, A., and Noguchi, J. 2010. Structural dynamics of dendritic spines in memory and cognition. Trends Neurosci. 33:121-129. doi:10.1016/j.tins.2010.01.001.
25. Knafo, S., Alonso-Nanclares, L., Gonzalez-Soriano, J., Merino-Serrais, P., Fernaud-Espinosa, I., Ferrer, I., and DeFelipe, J. 2009a. Widespread changes in dendritic spines in a model of Alzheimer’s disease. Cereb. Cortex 19:586-592. doi: 10.1093/cercor/bhn111.
26. Knafo, S., Venero, C., Merino-Serrais, P., Fernaud-Espinosa, I., Gonzalez-Soriano, J., Ferrer, I., Santpere, G., and DeFelipe, J. 2009b. Morphological alterations to neurons of the amygdala and impaired fear conditioning in a transgenic mouse model of Alzheimer’s disease. J. Pathol. 219:41-51. doi: 10.1002/path.2565.
27. Maze, I., Covington, H.E., 3rd, Dietz, D.M., La-Plant, Q., Renthal, W., Russo, S.J., Mechanic, M., Mouzon, E., Neve, R.L., Haggarty, S.J., Ren, Y., Sampath, S.C., Hurd, Y.L., Greengard, P., Tarakhovsky, A., Schaefer, A., and Nestler, E.J. 2010. Essential role of the histone methyltransferase G9a in cocaine-induced plasticity. Science 327: 213-216. doi: 10.1126/science.1179438.
28. Merino-Serrais, P., Knafo, S., Alonso-Nanclares, L., Fernaud-Espinosa, I., and DeFelipe, J. 2011. Layer-specific alterations to CA1 dendritic spines in a mouse model of Alzheimer’s disease. Hippocampus 21: 1037-1044. doi:10.1002/hipo.20861.
29. Nasse, M.J. and Woehl, J.C. 2010. Realistic modeling of the illumination point spread function in confocal scanning optical microscopy. J. Opt. Soc. Am. A. 27: 295-302. doi:10.1364/JOSAA.27.000295.
30. Nimchinsky, E.A., Sabatini, B.L., and Svoboda, K. 2002. Structure and function of dendritic spines. Annu. Rev. Physiol. 64: 313-353. doi:10.1146/annurev.physiol.64.081501.160008.
31. Peters, A. and Kaiserman-Abramof, I.R. 1970. The small pyramidal neuron of the rat cerebral cortex. The perikaryon, dendrites and spines. Am. J. Anat. 127: 321-355. doi: 10.1002/aja.1001270402.
32. Phillips, M. and Pozzo-Miller, L. 2015. Dendritic spine dysgenesis in autism related disorders. Neurosci. Lett. 601: 30-40. doi: 10.1016/j.neulet .2015.01.011.
33. Price, K.A., Varghese, M., Sowa, A., Yuk, F., Brautigam, H., Ehrlich, M.E., and Dickstein, D.L. 2014. Altered synaptic structure in the hippocampus in a mouse model of Alzheimer’s disease with soluble amyloid-beta oligomers and no plaque pathology. Mol. Neurodegener. 9: 41. doi: 10.1186/1750-1326-9-41.
34. Radley, J.J., Rocher, A.B., Rodriguez, A., Ehlenberger, D.B., Dammann, M., McEwen, B.S., Morrison, J.H., Wearne, S.L., and Hof, P.R. 2008. Repeated stress alters dendritic spine morphology in the rat medial prefrontal cortex. J. Comp. Neurol. 507: 1141-1150. doi:10.1002/cne.21588.
35. Ramos-Miguel, A., Barr, A.M., and Honer, W.G. 2015. Spines, synapses, and schizophrenia. Biol. Psychiatry 78: 741-743. doi: 10.1016/j.biopsych.2015.08.035.
36. Rocher, A.B., Crimins, J.L., Amatrudo, J.M., Kinson, M.S., Todd-Brown, M.A., Lewis, J., and Luebke, J.I. 2010. Structural and functional changes in tau mutant mice neurons are not linked to the presence of NFTs. Exp. Neurol. 223: 385-393. doi: 10.1016/j.expneurol .2009.07.029.
37. Rodriguez, A., Ehlenberger, D.B., Hof, P.R., and Wearne, S.L. 2006. Rayburst sampling, an algorithm for automated three-dimensional shape analysis from laser scanning microscopy images. Nat. Protoc. 1: 2152-2161. doi: 10.1038/nprot.2006.313.
38. Rodriguez, A., Ehlenberger, D.B., Dickstein, D.L., Hof, P.R., and Wearne, S.L. 2008. Automated three-dimensional detection and shape classification of dendritic spines from fluorescence microscopy images. PLoS One 3: e1997. doi: 10.1371/journal.pone.0001997.
39. Rodriguez, A., Ehlenberger, D., Kelliher, K., Einstein, M., Henderson, S.C., Morrison, J.H., Hof, P.R., and Wearne, S.L. 2003. Automated reconstruction of three-dimensional neuronal morphology from laser scanning microscopy images. Methods 30: 94-105. doi: 10.1016/S1046-2023(03)00011-2.
40. Selvas, A., Coria, S.M., Kastanauskaite, A., Fernaud-Espinosa, I., DeFelipe, J., Ambrosio, E., and Miguens, M. 2015. Rat-strain dependent changes of dendritic and spine morphology in the hippocampus after cocaine selfadministration. Addict. Biol. ePub ahead of print. doi: 10.1111/adb.12294.
41. Shansky, R.M., Hamo, C., Hof, P.R., McEwen, B.S., and Morrison, J.H. 2009. Stress-induced dendritic remodeling in the prefrontal cortex is circuit specific. Cereb. Cortex 19: 2479-2484. doi: 10.1093/cercor/bhp003.
42. Spacek, J. and Hartmann, M. 1983. Threedimensional analysis of dendritic spines. I. Quantitative observations related to dendritic spine and synaptic morphology in cerebral and cerebellar cortices. Anat. Embryol. 167: 289-310. doi: 10.1007/BF00298517.
43. Steele, J.W., Brautigam, H., Short, J.A., Sowa, A., Shi, M., Yadav, A., Weaver, C.M., Westaway, D., Fraser, P.E., St George-Hyslop, P.H., Gandy, S., Hof, P.R., and Dickstein, D.L. 2014. Early fear memory defects are associated with altered synaptic plasticity and molecular architecture in the TgCRND8 Alzheimer’s disease mouse model. J. Comp. Neurol. 522: 2319-2335. doi:10.1002/cne.23536.
44. Vecellio, M., Schwaller, B., Meyer, M., Hunziker, W., and Celio, M.R. 2000. Alterations in Purkinje cell spines of calbindin D-28 k and parvalbumin knock-out mice. Eur. J. Neurosci. 12: 945-954. doi: 10.1046/j.1460-9568.2000.00986.x.
45. Wallace,W. and Bear,M.F. 2004. A morphological correlate of synaptic scaling in visual cortex. J. Neurosci. 24: 6928-6938. doi: 10.1523/JNEU ROSCI.1110-04.2004.
46. Wearne, S.L., Rodriguez, A., Ehlenberger, D.B., Rocher, A.B., Henderson, S.C., and Hof, P.R. 2005. New techniques for imaging, digitization and analysis of three-dimensional neural morphology on multiple scales. Neuroscience 136:661-680. doi: 10.1016/j.neuroscience .2005.05.053.
47. https://www.youtube.com/watch?v=HczuQjeNcR4&feature=youtu.be
48. Drs. Dickstein and Tappan hosted a webinar on this topic based on a previous version of Neurolucida 360. The recorded version of the webinar is available for viewing here.
49. http://www.zeiss.com/microscopy/en_us/products/microscope-software/zen-lite.html
50. Zen Black image acquisition software described in the protocol. Free versions or free trials are available from the vendor.
51. http://www.mediacy.com/index.aspx?page=Auto Quant
52. AutoQuant image deconvolution software (Media Cybernetics) described in the protocol. Free versions or free tria4sls are available from the vendor.
53. http://www.mbfbioscience.com/neurolucida360
54. Neurolucida 360 automated neuron reconstruction software (MBF Bioscience) described in the protocol. Free versions or free trials are available from the vendor.