Dye coupling and immunostaining of astrocyte-like glia following intracellular injection of fluorochromes in brain slices of the grasshopper, schistocerca gregaria

Methods in Molecular Biology

Volumn 1082, Issue , 2014, Pages 99-113

  Boyan, George ^a   Liu, Yu ^a  

^a UNIVERSITY OF MUNICH   (Germany)

Author keywords

Brain slices; Dye injection; Gap junctions; Glia; Immunolabeling; Insect

Indexed keywords

FLUOROCHROME; GLUTAMATE AMMONIA LIGASE; HORSERADISH PEROXIDASE;

ANIMAL CELL; ANIMAL TISSUE; ANTIBODY LABELING; ARTICLE; ASTROCYTE; BRAIN SLICE; CELL INFILTRATION; CONTROLLED STUDY; EMBRYO; FLUORESCENCE MICROSCOPY; FLUOROCHROME STAINING; GAP JUNCTION; GLIA CELL; IMMUNOHISTOCHEMISTRY; LASER MICROSCOPY; MICROELECTRODE; MICROMANIPULATION; NEUROPIL; NONHUMAN; PRIORITY JOURNAL; SCHISTOCERCA GREGARIA; SURFACE TENSION; TEMPERATURE ACCLIMATIZATION; TISSUE PRESERVATION; VISUAL ORIENTATION;

ALEXA; HEXAPODA; SCHISTOCERCA GREGARIA;

ANIMALS; ASTROCYTES; BRAIN; CRYOPRESERVATION; FLUORESCENT DYES; GAP JUNCTIONS; GRASSHOPPERS; IMMUNOHISTOCHEMISTRY; INJECTIONS; INTRACELLULAR SPACE; MOLECULAR IMAGING; NEUROGLIA;

EID: 84934439774     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-655-9_7     Document Type: Article

References (41)

1. Jones BW, Fetter RD, Tear G, Goodman CS (1995) Glial cells missing: A genetic switch that controls glial versus neuronal fate. Cell 82: 1013-1023
2. Jones BW (2001) Glial cell development in the Drosophila embryo. Bioessays 23:877-887
3. Hidalgo A (2003) Neuron-glia interactions during axon guidance in Drosophila Biochem Soc Trans 31:50-55
4. Klämbt C (2009) Modes and regulation of glial migration in vertebrates and invertebrates. Nat Rev Neurosci 10:769-779
5. Vanhems E (1995) Insect glial cells and their relationships with neurons. In: Vernadakis A, Roots B (eds) Neuron-Glia interrelations during phylogeny: II. Plasticity and regeneration. Humana, Totowa, NJ, pp 49-77
6. Awasaki T, Lai S-L, Ito K, Lee T (2008) Organization and postembryonic development of glial cells in the adult central brain of Drosophila J Neurosci 28:13742-13753
7. Edwards TN, Meinertzhagen IA (2010) The functional organisation of glia in the adult brain of Drosophila and other insects. Prog Neurobiol 90:471-497
8. Awasaki T, Lee T (2011) New tools for the analysis of glial cell biology in Drosophila Glia 59:1377-1386
9. Boyan G, Loser M, Williams L, Liu Y (2011) Astrocyte-like glia associated with the embryonic development of the central complex in the grasshopper Schistocerca gregaria Dev Genes Evol 221:141-155
10. Schofi eld PK, Swales LS, Trehern JE (1984) Potentials associated with the blood-brain barrier of an insect: recordings from identifi ed neuroglia. J Exp Biol 109:307-318
11. Swales LS, Lane NJ (1985) Embyronic development of glial cells and their junctions in the locust central nervous system. J Neurosci 5:117-127
12. Schmidt J, Deitmar JW (1996) Photoinactivation of the giant neuropil glial cells in the leech Hirudo medicinalis : effects on neuronal activity and synaptic transmission. J Neurophysiol 76:2861-2871
13. Alexopoulos H, Böttger A, Fischer S et al (2004) Evolution of gap junctions: the missing link? Curr Biol 14:879-880
14. Phelan P, Goulding LA, Tam JLY et al (2008) Molecular mechanism of rectifi cation at an identifi ed electrical synapse in the Drosophila giant fi bre system. Curr Biol 18:1955-1960
15. Koussa MA, Tolbert LP, Oland LA (2011) Development of a glial network in the olfactory nerve: role of calcium and neuronal activity. Neuron Glia Biol 6(4):245-261
16. Zahs KR, Newman E (1997) Asymmetric gap junctional coupling between glial cells in the rat retina. Glia 20:10-22
17. Newman EA (2001) Propagation of intercellular calcium waves in retinal astrocytes and Müller cells. J Neurosci 21:2215-2223
18. Theiss C, Meller K (2002) Aluminum impairs gap junctional intercellular communication between astroglial cells in vitro. Cell Tissue Res 310:143-154
19. Houades V, Koulakoff A, Ezan P et al (2008) Gap junction-mediated astrocytic networks in the mouse barrel cortex. J Neurosci 28: 5207-5217
20. Tanaka M, Yamaguchi K, Tatsukawa T et al (2008) Connexin43 and bergmann glial gap junctions in cerebellar function. Front Neurosci 2:225-233
21. Parys B, Cote A, Gallo V et al (2010) Intercellular calcium signaling between astrocytes and oligodendrocytes via gap junctions in culture. Neuroscience 167:1032-1043
22. Rela L, Bordey A, Greer CA (2010) Olfactory ensheathing cell membrane properties are shaped by connectivity. Glia 58:665-678
23. Hossain MZ, Ernst LA, Nagy JI (1995) Utility of intensely fl uorescent cyanine dyes (Cy3) for assay of gap junctional communication by dyetransfer. Neurosci Lett 184:71-74
24. Ball KK, Gandhi GK, Thrash J et al (2007) Astrocytic connexin distributions and rapid, extensive dye transfer via gap junctions in the inferior colliculus: implications for [ 14 C] Glucose metabolite traffi cking. J Neurosci Res 85:3267-3283
25. Lanosa XA, Reisin HD, Santacroce I et al (2008) Astroglial dye-coupling: an in vitro analysis of regional and interspecies differences in rodents and primates. Brain Res 1240:82-86
26. Boyan GS, Liu Y, Loser M (2012) A cellular network of dye-coupled glia associated with the embryonic central complex in the grasshopper Schistocerca gregaria. Dev Genes Evol 222:125-138
27. Burt JM, Spray DC (1980) Single-channel events and gating behavior of the cardiac gap junction channel. Proc Natl Acad Sci U S A 85:3431-3434
28. Weingart R, Bukauskas FF (1998) Long-chain n-alkanols and arachidonic acid interfere with the Vm-sensitive gating mechanisms of gap junction channels. Eur J Physiol 435:310-319
29. Juszczak GR, Swiergiel AH (2009) Properties of gap junction blockers and their behavioural, cognitive and electrophysiological effects: animal and human studies. Prog Neuropsychopharmacol Biol Psychiatry 33:181-198
30. Boyan GS, Niederleitner B (2011) Patterns of dye coupling involving serotonergic neurons provide insights into the cellular organization of a central complex lineage of the embryonic grasshopper Schistocerca gregaria Dev Genes Evol 220:297-313
31. Chapman JA, Kirkness EF, Simakov O et al (2010) The dynamic genome of Hydra Nature 464:592-596
32. Wedler FC, Horn BR (1976) Catalytic mechanisms of glutamine synthetase enzymes. J Biol Chem 251:7530-7538
33. Martinez-Hernandez A, Bell KP, Norenberg MD (1977) Glutamine synthetase: glial localization in brain. Science 195:1356-1358
34. van der Hel WS, Notenboom RGE, Bos IWM et al (2005) Reduced glutamine synthetase in hippocampal areas with neuron loss in temporal lobe epilepsy. Neurology 64:326-333
35. Ward M, Jobling A, Puthussery T et al (2004) Localization and expression of the glutamate transporter, excitatory amino acid transporter 4, within astrocytes of the rat retina. Cell Tissue Res 315:305-310
36. Snow PM, Patel NH, Harrelson AL et al (1987) Neural-specifi c carbohydrate moiety shared by many surface glycoproteins in Drosophila and grasshopper embryos. J Neurosci 7:4137-4144
37. Jan LY, Jan YN (1982) Antibodies to horseradish-peroxidase as specifi c neuronal markers in Drosophila and grasshopper embryos. Proc Natl Acad Sci U S A 79:2700-2704
38. Haase A, Stern M, Wächtler K et al (2001) A tissue-specifi c marker of Ecdysozoa. Dev Genes Evol 211:428-433
39. Halter DA, Urban J, Rickert C et al (1995) The homeobox gene repo is required for the differentiation and maintenance of glial function in the embryonic nervous system of Drosophila melanogaster Development 121: 317-322
40. Bentley D, Keshishian H, Shankland M et al (1979) Quantitative staging of embryonic development of the grasshopper, Schistocerca nitens J Embryol Exp Morphol 54:47-74
41. Weber PA, Chang HC, Spaeth KE et al (2004) The permeability of gap junction channels to probes of different size is dependent on connexin composition and permeant-pore affi nities. Biophys J 87:958-973