Efficient, footprint-free human iPSC genome editing by consolidation of Cas9/CRISPR and piggyBac technologies

Nature Protocols

Volumn 12, Issue 1, 2017, Pages 88-103

  Wang, Gang ^a   Yang, Luhan ^b,c,e   Grishin, Dennis ^c   Rios, Xavier ^c   Ye, Lillian Y ^a   Hu, Yong ^a   Li, Kai ^a   Zhang, Donghui ^a   Church, George M ^b,c   Pu, William T ^a,d  

^a Department of Cardiology ^*  (United States)

^b HARVARD UNIVERSITY   (United States)

^c HARVARD MEDICAL SCHOOL   (United States)

^d HARVARD STEM CELL INSTITUTE   (United States)

^e EGenesis Biosciences   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CRISPR ASSOCIATED PROTEIN; DNA; DOXYCYCLINE; RNA; TRANSPOSASE;

ARTICLE; CLINICAL PROTOCOL; CONTROLLED STUDY; CRISPP GENE; DNA REPAIR; ENCAPSULATION; GENE CONSOLIDATION; GENE EDITING; GENE FREQUENCY; GENE MUTATION; GENETIC ENGINEERING; GENETIC TRANSFECTION; HOMOLOGY DIRECTED REPAIR; HUMAN; HUMAN CELL; INDEL MUTATION; INDUCED PLURIPOTENT STEM CELL; MOLECULAR CLONING; MUTAGENESIS; PRIORITY JOURNAL; PROCESS DEVELOPMENT; QUALITY CONTROL; TRANSPOSON; CELL LINE; CRISPR CAS SYSTEM; DRUG EFFECTS; GENETICS; HUMAN GENOME; METABOLISM; NUCLEOTIDE SEQUENCE; PROCEDURES;

BASE SEQUENCE; CELL LINE; CRISPR-CAS SYSTEMS; DOXYCYCLINE; GENE EDITING; GENOME, HUMAN; HUMANS; INDUCED PLURIPOTENT STEM CELLS;

EID: 85020899239     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2016.152     Document Type: Article

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