Assessment of long-term cultivated human precision-cut lung slices as an ex vivo system for evaluation of chronic cytotoxicity and functionality

Journal of Occupational Medicine and Toxicology

Volumn 12, Issue 1, 2017, Pages

  Neuhaus, Vanessa ^a,b   Schaudien, Dirk ^a,b   Golovina, Tatiana ^c   Temann, Ulla Angela ^c   Thompson, Carolann ^c   Lippmann, Torsten ^b,d   Bersch, Claus ^e   Pfennig, Olaf ^e   Jonigk, Danny ^d   Braubach, Peter ^d   Fieguth, Hans Gerd ^f   Warnecke, Gregor ^b,d   Yusibov, Vidadi ^b   Sewald, Katherina ^a,b   Braun, Armin ^a,d  

^a FRAUNHOFER INSTITUTE FOR TOXICOLOGY AND EXPERIMENTAL MEDICINE   (Germany)

^b German Center for Lung Research   (Germany)

^c Fraunhofer USA Inc   (United States)

^d HANNOVER MEDICAL SCHOOL   (Germany)

^e Nordstadtkrankenhaus   (Germany)

^f Division of Thoracic and Vascular Surgery   (Germany)

Author keywords

Bronchoconstriction; Cytokines; Cytotoxicity; Human; Long term cultivation; PCLS; Precision cut lung slices

Indexed keywords

TUMOR NECROSIS FACTOR;

ADULT; ARTICLE; BRONCHOCONSTRICTION; CHRONIC CYTOTOXICITY; CONTROLLED STUDY; CYTOKINE RELEASE; CYTOTOXICITY; EC50; EX VIVO STUDY; FEASIBILITY STUDY; HUMAN; HUMAN CELL; HUMAN TISSUE; INITIAL AIRWAY AREA; LUNG CULTURE; LUNG FUNCTION; LUNG SLICE; MIDDLE AGED; PARAMETERS; PRECISION CUT LUNG SLICE; RESPIRATORY TRACT PARAMETERS; SENSITIVITY ANALYSIS; STRUCTURAL INTEGRITY; TISSUE CULTURE; TISSUE VIABILITY;

EID: 85020305921     PISSN: None     EISSN: 17456673     Source Type: Journal    
DOI: 10.1186/s12995-017-0158-5     Document Type: Article

References (35)

1. World Health Organization (WHO). The top 10 causes of death. Fact Sheet. Available at: http://www.who.int/mediacentre/factsheets/fs310/en/index2.html. [Accessed 8-1-2016].
2. World Health Organization (WHO). Noncommunicable diseases. Fact Sheet. Available at: http://www.who.int/mediacentre/factsheets/fs355/en/. [Accessed 8-1-2016].
3. Sewald K, Braun A. Assessment of immunotoxicity using precision-cut tissue slices. Xenobiotica. 2013;43(1):84-97. doi: 10.3109/00498254.2012.731543.
4. Brown SM, Koarai A, Sturton RG, Nicholson AG, Barnes PJ, Donnelly LE. A role for M(2) and M(3) muscarinic receptors in the contraction of rat and human small airways. Eur J Pharmacol. 2013;702(1-3):109-15. doi: 10.1016/j.ejphar.2013.01.054.
5. Henjakovic M, Sewald K, Switalla S, Kaiser D, Muller M, Veres TZ, et al. Ex vivo testing of immune responses in precision-cut lung slices. Toxicol Appl Pharmacol. 2008;231(1):68-76. doi: 10.1016/j.taap.2008.04.003.
6. Schleputz M, Rieg AD, Seehase S, Spillner J, Perez-Bouza A, Braunschweig T, et al. Neurally mediated airway constriction in human and other species: a comparative study using precision-cut lung slices (PCLS). PLoS One. 2012;7(10):e47344. doi: 10.1371/journal.pone.0047344. PONE-D-12-11366 [pii]
7. Switalla S, Lauenstein L, Prenzler F, Knothe S, Forster C, Fieguth HG, et al. Natural innate cytokine response to immunomodulators and adjuvants in human precision-cut lung slices. Toxicol Appl Pharmacol 2010;246(3):107-115; 10.1016/j.taap.2010.04.010.
8. Fisher RL, Smith MS, Hasal SJ, Hasal KS, Gandolfi AJ, Brendel K. The use of human lung slices in toxicology. Hum Exp Toxicol. 1994;13(7):466-71.
9. Lauenstein L, Switalla S, Prenzler F, Seehase S, Pfennig O, Forster C, et al. Assessment of immunotoxicity induced by chemicals in human precision-cut lung slices (PCLS). Toxicol in Vitro. 2014;28(4):588-99. doi: 10.1016/j.tiv.2013.12.016.
10. Ressmeyer AR, Larsson AK, Vollmer E, Dahlen SE, Uhlig S, Martin C. Characterisation of guinea pig precision-cut lung slices: comparison with human tissues. Eur Respir J. 2006;28(3):603-11. doi: 10.1183/09031936.06.00004206.
11. Rieg AD, Suleiman S, Perez-Bouza A, Braunschweig T, Spillner JW, Schroder T, et al. Milrinone relaxes pulmonary veins in Guinea pigs and humans. PLoS One. 2014;9(1):e87685. doi: 10.1371/journal.pone.0087685.
12. Nassimi M, Schleh C, Lauenstein HD, Hussein R, Hoymann HG, Koch W, et al. A toxicological evaluation of inhaled solid lipid nanoparticles used as a potential drug delivery system for the lung. Eur J Pharm Biopharm. 2010;75(2):107-16. doi: 10.1016/j.ejpb.2010.02.014.
13. Sauer UG, Vogel S, Aumann A, Hess A, Kolle SN, Ma-Hock L, et al. Applicability of rat precision-cut lung slices in evaluating nanomaterial cytotoxicity, apoptosis, oxidative stress, and inflammation. Toxicol Appl Pharmacol. 2014;276(1):1-20. doi: 10.1016/j.taap.2013.12.017.
14. Dobrescu I, Levast B, Lai K, Delgado-Ortega M, Walker S, Banman S, et al. In vitro and ex vivo analyses of co-infections with swine influenza and porcine reproductive and respiratory syndrome viruses. Vet Microbiol. 2014;169(1-2):18-32. doi: 10.1016/j.vetmic.2013.11.037.
15. Umachandran M, Howarth J, Ioannides C. Metabolic and structural viability of precision-cut rat lung slices in culture. Xenobiotica. 2004;34(8):771-80. doi: 10.1080/00498250400000816.
16. Williams PP, Gallagher JE. Preparation and long-term cultivation of porcine tracheal and lung organ cultures by alternate exposure to gaseous and liquid medium phases. In Vitro. 1978;14(8):686-96.
17. Siminski JT, Kavanagh TJ, Chi E, Raghu G. Long-term maintenance of mature pulmonary parenchyma cultured in serum-free conditions. Am J Phys. 1992;262(1 Pt 1):L105-10.
18. Shapiro P, Absher PM, Casty F, Evans J. Maintaining intact mature lung tissue in culture using low melt agarose. Methods Cell Sci. 1995;17(4):245-9. http://dx.doi.org/10.1007/BF00986229
19. Temann A, Golovina T, Neuhaus V, Thompson C, Chichester JA, Braun A, et al. Evaluation of inflammatory and immune responses in long-term cultured human precision-cut lung slices. Hum Vaccin Immunother. 2017;13(2), S.):351-8.
20. Hansen NU, Karsdal MA, Brockbank S, Cruwys S, Ronnow S, Leeming DJ. Tissue turnover of collagen type I, III and elastin is elevated in the PCLS model of IPF and can be restored back to vehicle levels using a phosphodiesterase inhibitor. Respir Res. 2016;17(1):76. doi: 10.1186/s12931-016-0394-8. 10.1186/s12931-016-0394-8 [pii]
21. Seehase S, Schleputz M, Switalla S, Matz-Rensing K, Kaup FJ, Zoller M, et al. Bronchoconstriction in nonhuman primates: a species comparison. J Appl Physiol (1985). 2011;111(3):791-8. doi: 10.1152/japplphysiol.00162.2011.
22. Switalla S, Knebel J, Ritter D, Krug N, Braun A, Sewald K. Effects of acute in vitro exposure of murine precision-cut lung slices to gaseous nitrogen dioxide and ozone in an air-liquid interface (ALI) culture. Toxicol Lett. 2010;196(2):117-24. doi: 10.1016/j.toxlet.2010.04.004.
23. Berndt WO. Use of the tissue slice technique for evaluation of renal transport processes. Environ Health Perspect. 1976;15:73-88.
24. Rahman MM, McFadden G. Modulation of tumor necrosis factor by microbial pathogens. PLoS Pathog. 2006;2(2):e4. doi: 10.1371/journal.ppat.0020004.
25. Sekine-Okano M, Lucas R, Rungger D, De KT, Grau GE, Leuenberger PM, et al. Expression and release of tumor necrosis factor-alpha by explants of mouse cornea. Invest Ophthalmol Vis Sci. 1996;37(7):1302-10.
26. Eischen A, Vincent F, Bergerat JP, Louis B, Faradji A, Bohbot A, et al. Long term cultures of human monocytes in vitro. Impact of GM-CSF on survival and differentiation. J Immunol Methods. 1991;143(2):209-21.
27. Kreutz M, Krause SW, Hennemann B, Rehm A, Andreesen R. Macrophage heterogeneity and differentiation: defined serum-free culture conditions induce different types of macrophages in vitro. Res Immunol. 1992;143(1):107-15.
28. Wouters IM, Douwes J, Thorne PS, Heederik D, Doekes G. Inter- and intraindividual variation of endotoxin- and beta(1 - > 3)-glucan-induced cytokine responses in a whole blood assay. Toxicol Ind Health. 2002;18(1):15-27.
29. Behrsing HP, Furniss MJ, Davis M, Tomaszewski JE, Parchment RE. In vitro exposure of precision-cut lung slices to 2-(4-amino-3-methylphenyl)-5-fluorobenzothiazole lysylamide dihydrochloride (NSC 710305, Phortress) increases inflammatory cytokine content and tissue damage. Toxicol Sci. 2013;131(2):470-9. doi: 10.1093/toxsci/kfs319.
30. Cooper PR, Zhang J, Damera G, Hoshi T, Zopf DA, Panettieri RA Jr. C-027 inhibits IgE-mediated passive sensitization bronchoconstriction and acts as a histamine and serotonin antagonist in human airways. Allergy Asthma Proc. 2011;(5):359-65. doi: 10.2500/aap.2011.32.3460.
31. Delgado-Ortega M, Melo S, Punyadarsaniya D, Rame C, Olivier M, Soubieux D, et al. Innate immune response to a H3N2 subtype swine influenza virus in newborn porcine trachea cells, alveolar macrophages, and precision-cut lung slices. Vet Res. 2014;45:42.1297-9716-45-42 [pii]. doi: 10.1186/1297-9716-45-42.
32. Lambermont VA, Schleputz M, Dassow C, Konig P, Zimmermann LJ, Uhlig S, et al. Comparison of Airway Responses in Sheep of Different Age in Precision-Cut Lung Slices (PCLS). PLoS One. 2014;9(9) e97610.10.1371/journal.pone.0097610
33. Seehase S, Lauenstein HD, Schlumbohm C, Switalla S, Neuhaus V, Forster C, et al. LPS-induced lung inflammation in marmoset monkeys - an acute model for anti-inflammatory drug testing. PLoS One. 2012;7(8):e43709. doi: 10.1371/journal.pone.0043709. PONE-D-12-00746 [pii]
34. Kasper M, Seidel D, Knels L, Morishima N, Neisser A, Bramke S, et al. Early signs of lung fibrosis after in vitro treatment of rat lung slices with CdCl2 and TGF-beta1. Histochem Cell Biol. 2004;121(2):131-40. doi: 10.1007/s00418-003-0612-6.
35. Lin CJ, Yang PC, Hsu MT, Yew FH, Liu TY, Shun CT, et al. Induction of pulmonary fibrosis in organ-cultured rat lung by cadmium chloride and transforming growth factor-beta1. Toxicology. 1998;127(1-3):157-66.