Rutin exhibits hepatoprotective effects in a mouse model of non-alcoholic fatty liver disease by reducing hepatic lipid levels and mitigating lipid-induced oxidative injuries

International Immunopharmacology

Volumn 49, Issue , 2017, Pages 132-141

  Liu, Qingsheng ^a   Pan, Ran ^a,b   Ding, Lei ^a   Zhang, Fuli ^a   Hu, Linfeng ^a   Ding, Bin ^a   Zhu, Linwensi ^c   Xia, Yongliang ^a   Dou, Xiaobing ^a  

^a ZHEJIANG CHINESE MEDICAL UNIVERSITY   (China)

^b ZHEJIANG UNIVERSITY SCHOOL OF MEDICINE   (China)

^c SHANGHAI JIAO TONG UNIVERSITY SCHOOL OF MEDICINE   (China)

Author keywords

Autophagy; Lipid synthesis and metabolism; Oleic acid; Rutin

Indexed keywords

ACETYL COENZYME A CARBOXYLASE; BIOLOGICAL MARKER; CARNITINE PALMITOYLTRANSFERASE; CARNITINE PALMITOYLTRANSFERASE 2; CARNITINE PALMITOYLTRANSFERASE I; DIACYLGLYCEROL ACYLTRANSFERASE 1; DIACYLGLYCEROL ACYLTRANSFERASE 2; FAT DROPLET; INTERLEUKIN 1BETA; LIPID; MALONALDEHYDE; OLEIC ACID; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR ALPHA; REACTIVE OXYGEN METABOLITE; RUTOSIDE; STEROL REGULATORY ELEMENT BINDING PROTEIN 1C; SUPEROXIDE DISMUTASE; TRIACYLGLYCEROL; TUMOR NECROSIS FACTOR; UNCLASSIFIED DRUG;

ADIPOGENESIS; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; AUTOPHAGY; CELL PROLIFERATION; CELL VIABILITY; CONCENTRATION RESPONSE; CONTROLLED STUDY; DOWN REGULATION; ENZYME ACTIVITY; ENZYME LINKED IMMUNOSORBENT ASSAY; FAT INTAKE; FATTY ACID METABOLISM; FATTY ACID TRANSPORT; HEP-G2 CELL LINE; HUMAN; HUMAN CELL; LIPID DIET; LIPID LIVER LEVEL; LIPID STORAGE; LIPOLYSIS; LIVER CELL; LIVER PROTECTION; LIVER TISSUE; MALE; MOUSE; MTT ASSAY; NONALCOHOLIC FATTY LIVER; NONHUMAN; OPTIMAL DRUG DOSE; OXIDATIVE STRESS; PRIORITY JOURNAL; PROTEIN CONTENT; PROTEIN EXPRESSION; QUANTITATIVE ASSAY; RAW 264.7 CELL LINE; REAL TIME POLYMERASE CHAIN REACTION; STAINING; TRIACYLGLYCEROL BLOOD LEVEL; UPREGULATION; WEIGHT GAIN; ANIMAL; C57BL MOUSE; CELL PROTECTION; DISEASE MODEL; GENETICS; LIPID METABOLISM; LIPID PEROXIDATION; LIVER; METABOLISM; PATHOLOGY;

ANIMALS; CARNITINE O-PALMITOYLTRANSFERASE; CYTOPROTECTION; DIET, HIGH-FAT; DISEASE MODELS, ANIMAL; HEP G2 CELLS; HUMANS; LIPID METABOLISM; LIPID PEROXIDATION; LIVER; MALE; MICE; MICE, INBRED C57BL; NON-ALCOHOLIC FATTY LIVER DISEASE; OXIDATIVE STRESS; PPAR ALPHA; REACTIVE OXYGEN SPECIES; RUTIN;

EID: 85020000084     PISSN: 15675769     EISSN: 18781705     Source Type: Journal    
DOI: 10.1016/j.intimp.2017.05.026     Document Type: Article

References (38)

1. Bedogni, G., Miglioli, L., Masutti, F., Tiribelli, C., Marchesini, G., Bellentani, S., Prevalence of and risk factors for nonalcoholic fatty liver disease: the dionysos nutrition and liver study[J]. Hepatology 42 (2005), 44–52.
2. Targher, G., Bertolini, L., Rodella, S., Tessari, R., Zenari, L., Lippi, G., Nonalcoholic fatty liver disease is independently associated with an increased incidence of cardiovascular events in type 2 diabetic patients[J]. Diabetes Care 30 (2007), 2119–2121.
3. Chalasani, N., Younossi, Z., Lavine, J.E., Diehl, A.M., Brunt, E.M., Cusi, K., The diagnosis and management of non-alcoholic fatty liver disease: practice guideline by the American Association for the Study of Liver Diseases, American College of Gastroenterology, and the American Gastroenterological Association[J]. Hepatology 107 (2012), 811–826.
4. Baffy, G., Brunt, E.M., Caldwell, S.H., Hepatocellular carcinoma in non-alcoholic fatty liver disease: an emerging menace[J]. J. Hepatol. 56 (2012), 1384–1391.
5. Michelotti, G.A., Machado, M.V., Diehl, A.M., NAFLD, NASH and liver cancer[J]. Nat. Rev. Gastroenterol. Hepatol. 10 (2013), 656–665.
6. Day, C.P., James OF, Steatohepatitis: a tale of two "hits"?[J]. Gastroenterology 114 (1998), 842–845.
7. Marchesini, G., Brizi, M., Morsellilabate, A.M., Bianchi, G., Bugianesi, E., Mccullough, A.J., Association of nonalcoholic fatty liver disease with insulin resistance[J]. Am. J. Med. 107 (1999), 450–455.
8. Bugianesi, E., Moscatiello, S., Ciaravella, M.F., Marchesini, G., Insulin resistance in nonalcoholic fatty liver disease[J]. Curr. Pharm. Des. 16 (2010), 1941–1951.
9. Xu, L., Wei, Y., Dong, D., Yin, L., Qi, Y., Han, X., iTRAQ-based proteomics for studying the effects of dioscin against nonalcoholic fatty liver disease in rats[J]. RSC Adv. 4:58 (2014), 30704–30711.
10. Liu, M., Xu, L., Yin, L., Yan, Q., Xu, Y., Xu, H., Potent effects of dioscin against obesity in mice[J]. Sci. Rep., 5, 2015, 7973.
11. Wu, C.H., Lin, M.C., Wang, H.C., Yang, M.Y., Jou, M.J., Wang, C.J., Rutin inhibits oleic acid induced lipid accumulation via reducing lipogenesis and oxidative stress in Hepatocarcinoma cells[J]. J. Food Sci. 76 (2011), 65–72.
12. La, C.C., Villegas, I., dlLC, Alarcón, Motilva, V., Martín Calero, M.J., Evidence for protective and antioxidant properties of rutin, a natural flavone, against ethanol induced gastric lesions[J]. J. Ethnopharmacol. 71 (2000), 45–53.
13. Gao, M., Ma, Y., Liu, D., Rutin suppresses palmitic acids-triggered inflammation in macrophages and blocks high fat diet-induced obesity and fatty liver in mice[J]. Pharm. Res. 30 (2013), 2940–2950.
14. Lee, C.C., Shen, S.R., Lai, Y.J., Wu, S.C., Rutin and quercetin, bioactive compounds from tartary buckwheat, prevent liver inflammatory injury[J]. Food Funct., 4, 2013, 794.
15. 2 + [J]. J. Sichuan Univ. 35 (2004), 836–838.
16. Hsu, C.L., Wu, C.H., Huang, S.L., Yen, G.C., Phenolic compounds rutin and ocoumaric acid ameliorate obesity induced by high-fat diet in rats[J]. J. Agric. Food Chem. 57 (2009), 425–431.
17. Molina, M.F., Sanchez-Reus, I., Iglesias, I., Benedi, J., Quercetin, a flavonoid antioxidant, prevents and protects against ethanol-induced oxidative stress in mouse liver[J]. Biol. Pharm. Bull., 26, 2003, 1398.
18. Yin, J.J., Luo, Y.Q., Deng, H.L., Qin, S.M., Tang, W.J., Zeng, L., Hugan Qingzhi medication ameliorates hepatic steatosis by activating AMPK and PPARα pathways in L02 cells and HepG2 cells[J]. J. Ethnopharmacol., 14, 2014.
19. Niture, N.T., Ansari, A.A., Naik, S.R., Anti-hyperglycemic activity of Rutin in streptozotocin-induced diabetic rats: an effect mediated through cytokines, antioxidants and lipid biomarkers[J]. Indian J. Exp. Biol. 52 (2014), 720–727.
20. Xin, N., Qi, Y.J., Han, S.Y., Chu, J.X., Effect of total flavones of buckwheat flower on type two diabetic rat hyperlipidemia[J]. Chin. J. Clin. Rehab. 8 (2004), 5984–5985.
21. Wang, Z., Yao, T., Pini, M., Zhou, Z., Fantuzzi, G., Song, Z., Betaine improved adipose tissue function in mice fed a high-fat diet: a mechanism for hepatoprotective effect of betaine in nonalcoholic fatty liver disease[J]. Am. J. Physiol. Gastrointest. Liver Physiol. 298 (2010), G634–G642.
22. Issemann, I., Green, S., Activation of a member of the steroid hormone receptor superfamily by peroxisome proliferators[J]. Nature 347 (1990), 1155–1208.
23. Schoonjans, K., Staels, B., Auwerx, J., The peroxisome proliferator activated receptors (PPARs) and their effects on lipid metabolism and adipocyte differentiation[J]. Biochim. Biophys. Acta 1302 (1996), 93–109.
24. Chinetti, G., Fruchart, J.C., Staels, B., Peroxisome proliferator-activated receptors (PPARs): nuclear receptors at the crossroads between lipid metabolism and inflammation[J]. Inflamm. Res. 49 (2000), 497–505.
25. Kliewer, S.A., Sundseth, S.S., Jones, S.A., Brown, P.J., Wisely, G.B., Koble, C.S., Fatty acids and eicosanoids regulate gene expression through direct interactions with peroxisome proliferator-activated receptors alpha and gamma[J]. Proc. Natl. Acad. Sci. U. S. A. 94 (1997), 4318–4323.
26. Wu, C.H., Lin, M.C., Wang, H.C., Yang, M.Y., Jou, M.J., Wang, C.J., Rutin inhibits oleic acid induced lipid accumulation via reducing lipogenesis and oxidative stress in hepatocarcinoma cells[J]. J. Food Sci. 76 (2011), T65–T72.
27. Bonnefont, J.P., Djouadi, F., Prip-Buus, C., Gobin, S., Munnich, A., Bastin, J., Carnitine palmitoyltransferases 1 and 2: biochemical, molecular and medical aspects[J]. Mol. Asp. Med. 25 (2004), 495–520.
28. Brown, M.S., Goldstein, J.L., The SREBP pathway: regulation of cholesterol metabolism by proteolysis of a membrane-bound transcription factor[J]. Cell 89 (1997), 331–340.
29. Edwards, P.A., Tabor, D., Kast, H.R., Venkateswaran, A., Regulation of gene expression by SREBP and SCAP[J]. Biochim. Biophys. Acta 1529 (2001), 103–113.
30. Lavallard, V.J., Gual, P., Autophagy and non-alcoholic fatty liver disease[J]. Biomed. Res. Int., 2013, 2014, 120179.
31. Eberlé, D., Hegarty, B., Bossard, P., Ferré, P., Foufelle, F., SREBP transcription factors: master regulators of lipid homeostasis[J]. Biochimie 86 (2004), 839–848.
32. Chuffa, L.G., Fioruci-Fontanelli, B.A., Bordon, J.G., Pires, R.B., Braga, C.P., Seiva, F.R., Rutin ameliorates glycemic index, lipid profile and enzymatic activities in serum, heart and liver tissues of rats fed with a combination of hypercaloric diet and chronic ethanol consumption[J]. Indian J. Biochem. Biophys. 51 (2014), 215–222.
33. Hosseinzadeh, H., Nassiri-Asl, M., Review of the protective effects of rutin on the metabolic function as an important dietary flavonoid[J]. J. Endocrinol. Investig. 37 (2014), 783–788.
34. Chen, C., Krausz, K.W., Shah, Y.M., Idle, J.R., Gonzalez, F.J., Serum metabolomics reveals irreversible inhibition of fatty acid beta-oxidation through the suppression of PPAR alpha activation as a contributing mechanism of acetaminophen-induced hepatotoxicity[J]. Chem. Res. Toxicol. 22 (2009), 699–707.
35. Yoon, M., The role of PPAR alpha in lipid metabolism and obesity: focusing on the effects of estrogen on PPAR alpha actions[J]. Pharmacol. Res. Off. J. Italian Pharmacol. Soc. 60 (2009), 151–159.
36. Kim, S., Seo, S., Lee, M.S., Jang, E., Shin, Y., Oh, S., Rutin reduces body weight with an increase of muscle mitochondria biogenesis and activation of AMPK in diet-induced obese rats[J]. Toxicol. Sci. 8144:1 (2007), 4305–4310.
37. Ikeda, S., Miyazaki, H., Nakatani, T., Kai, Y., Kamei, Y., Miura, S., Up-regulation of SREBP-1c and lipogenic genes in skeletal muscles after exercise training[J]. Biochem. Biophys. Res. Commun. 296 (2002), 395–400.
38. Sinha, R.A., Farah, B.L., Singh, B.K., Siddique, M.M., Li, Y., Wu, Y., Caffeine stimulates hepatic lipid metabolism by the autophagy-lysosomal pathway in mice[J]. Hepatology 59:4 (2014), 1366–1380.