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RNA sequencing in 70 arthropod species uncovered 112 novel negative-sense RNA viruses that seem to be ancestral to negative-sense RNA viruses of vertebrates. Together with Refs. [4,5] from the same authors, this study places invertebrates central in the evolution of animal viruses.
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3•• Li, C.X., Shi, M., Tian, J.H., Lin, X.D., Kang, Y.J., Chen, L.J., Qin, X.C., Xu, J., Holmes, E.C., Zhang, Y.Z., Unprecedented genomic diversity of RNA viruses in arthropods reveals the ancestry of negative-sense RNA viruses. eLife, 4, 2015, 10.7554/eLife.05378 RNA sequencing in 70 arthropod species uncovered 112 novel negative-sense RNA viruses that seem to be ancestral to negative-sense RNA viruses of vertebrates. Together with Refs. [4,5] from the same authors, this study places invertebrates central in the evolution of animal viruses.
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An extensive metagenomic approach in 220 invertebrate species identified 1445 novel viruses, filling major gaps in phylogenies of known virus families and identifying putative novel virus families. This study finds evidence for frequent recombination between diverse viruses and uncovered greater diversity in viral genome organization than previously anticipated.
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Using RNA sequencing in diverse invertebrate species, this study found that the segmented jingmenviruses form a separate clade close the Flavivirus genus and identified distant relatives of members of the Flaviviridae family. The authors propose that the novel Flavi-like viruses in arthropods are ancestral viruses from which vertebrate-infecting viruses evolved.
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5• Shi, M., Lin, X.D., Vasilakis, N., Tian, J.H., Li, C.X., Chen, L.J., Eastwood, G., Diao, X.N., Chen, M.H., Chen, X., et al. Divergent viruses discovered in arthropods and vertebrates revise the evolutionary history of the flaviviridae and related viruses. J. Virol. 90 (2015), 659–669 Using RNA sequencing in diverse invertebrate species, this study found that the segmented jingmenviruses form a separate clade close the Flavivirus genus and identified distant relatives of members of the Flaviviridae family. The authors propose that the novel Flavi-like viruses in arthropods are ancestral viruses from which vertebrate-infecting viruses evolved.
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Shi, M.1
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6 Nguyet, M.N., Duong, T.H., Trung, V.T., Nguyen, T.H., Tran, C.N., Long, V.T., Dui le, T., Nguyen, H.L., Farrar, J.J., Holmes, E.C., et al. Host and viral features of human dengue cases shape the population of infected and infectious Aedes aegypti mosquitoes. Proc. Natl. Acad. Sci. U. S. A. 110 (2013), 9072–9077.
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7 Duong, V., Lambrechts, L., Paul, R.E., Ly, S., Lay, R.S., Long, K.C., Huy, R., Tarantola, A., Scott, T.W., Sakuntabhai, A., et al. Asymptomatic humans transmit dengue virus to mosquitoes. Proc. Natl. Acad. Sci. U. S. A. 112 (2015), 14688–14693.
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A comprehensive review that covers the discovery, transmission, host range, and genome organization of insect-specific flaviviruses.
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Evolutionary and phenotypic analysis of live virus isolates suggests arthropod origin of a pathogenic RNA virus family
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The identification of novel families within the order Bunyavirales combined with ancestral host reconstruction led to the conclusion that arthropod-borne bunyaviruses evolved from insect-specific ancestors. In addition, mosquito-specific viruses were shown to be temperature sensitive, with a complete block in replication at temperatures above 33°C. This observation may form the basis for a simple assay to distinguish invertebrate-specific viruses from arboviruses also in other virus families.
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9•• Marklewitz, M., Zirkel, F., Kurth, A., Drosten, C., Junglen, S., Evolutionary and phenotypic analysis of live virus isolates suggests arthropod origin of a pathogenic RNA virus family. Proc. Natl. Acad. Sci. U. S. A. 112 (2015), 7536–7541 The identification of novel families within the order Bunyavirales combined with ancestral host reconstruction led to the conclusion that arthropod-borne bunyaviruses evolved from insect-specific ancestors. In addition, mosquito-specific viruses were shown to be temperature sensitive, with a complete block in replication at temperatures above 33°C. This observation may form the basis for a simple assay to distinguish invertebrate-specific viruses from arboviruses also in other virus families.
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One of two studies [10,33] that used infectious cDNA clones of mosquito-specific viruses to generate chimeras with mosquito-borne viruses of the same genus to study host restriction in vertebrate cells.
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11 Nasar, F., Palacios, G., Gorchakov, R.V., Guzman, H., Da Rosa, A.P., Savji, N., Popov, V.L., Sherman, M.B., Lipkin, W.I., Tesh, R.B., et al. Eilat virus, a unique alphavirus with host range restricted to insects by RNA replication. Proc. Natl. Acad. Sci. U. S. A. 109 (2012), 14622–14627.
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14 Auguste, A.J., Kaelber, J.T., Fokam, E.B., Guzman, H., Carrington, C.V., Erasmus, J.H., Kamgang, B., Popov, V.L., Jakana, J., Liu, X., et al. A newly isolated reovirus has the simplest genomic and structural organization of any reovirus. J. Virol. 89 (2015), 676–687.
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15 Charles, J., Firth, A.E., Lorono-Pino, M.A., Garcia-Rejon, J.E., Farfan-Ale, J.A., Lipkin, W.I., Blitvich, B.J., Briese, T., Merida virus, a putative novel rhabdovirus discovered in Culex and Ochlerotatus spp. mosquitoes in the Yucatan Peninsula of Mexico. J. Gen. Virol. 97 (2016), 977–987.
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16 Fauver, J.R., Grubaugh, N.D., Krajacich, B.J., Weger-Lucarelli, J., Lakin, S.M., Fakoli, L.S. 3rd, Bolay, F.K., Diclaro, J.W. 2nd, Dabire, K.R., Foy, B.D., et al. West African Anopheles gambiae mosquitoes harbor a taxonomically diverse virome including new insect-specific flaviviruses, mononegaviruses, and totiviruses. Virology 498 (2016), 288–299.
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17 Harrison, J.J., Warrilow, D., McLean, B.J., Watterson, D., O'Brien, C.A., Colmant, A.M., Johansen, C.A., Barnard, R.T., Hall-Mendelin, S., Davis, S.S., et al. A new orbivirus isolated from mosquitoes in north-western Australia shows antigenic and genetic similarity to Corriparta virus but does not replicate in vertebrate cells. Viruses, 8, 2016, 10.3390/v8050141.
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