Efficient reverse genetic systems for rapid genetic manipulation of emergent and preemergent infectious coronaviruses

Methods in Molecular Biology

Volumn 1602, Issue , 2017, Pages 59-81

  Cockrell, Adam S ^a   Beall, Anne ^b   Yount, Boyd ^a   Baric, Ralph ^a,b  

^a The University of North Carolina at Chapel Hill   (United States)

^b UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

Author keywords

Bat coronavirus; Coronavirus (CoV); Emerging; Middle East respiratory syndrome coronavirus (MERS CoV); Porcine epidemic diarrhea virus (PEDV); Preemergent; Reverse genetics; Severe acute respiratory syndrome coronavirus (SARS CoV)

Indexed keywords

DNA FRAGMENT; NUCLEOTIDE; RESTRICTION ENDONUCLEASE; VIRUS RNA; COMPLEMENTARY DNA;

ANIMAL EXPERIMENT; ANIMAL MODEL; CORONAVIRIDAE; GENE FUNCTION; GENETIC MANIPULATION; GENOMIC INSTABILITY; HOST RANGE; MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS; NONHUMAN; PATHOGENESIS; PLASMID; RESTRICTION SITE; REVERSE GENETICS; VIRUS GENOME; ANIMAL; CHLOROCEBUS AETHIOPS; COMMUNICABLE DISEASE; CORONAVIRINAE; CORONAVIRUS INFECTION; GENE EXPRESSION REGULATION; GENETIC ENGINEERING; GENETIC RECOMBINATION; GENETIC TRANSFECTION; GENETICS; HUMAN; PROCEDURES; TRANSMISSION; VERO CELL LINE; VIROLOGY;

ANIMALS; CERCOPITHECUS AETHIOPS; COMMUNICABLE DISEASES, EMERGING; CORONAVIRUS; CORONAVIRUS INFECTIONS; DNA, COMPLEMENTARY; GENE EXPRESSION REGULATION, VIRAL; GENETIC ENGINEERING; GENOME, VIRAL; HUMANS; PLASMIDS; RECOMBINATION, GENETIC; REVERSE GENETICS; RNA, VIRAL; TRANSFECTION; VERO CELLS;

EID: 85019552784     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-6964-7_5     Document Type: Chapter

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