Cell fixation and preservation for droplet-based single-cell transcriptomics

BMC Biology

Volumn 15, Issue 1, 2017, Pages

  Alles, Jonathan ^a   Karaiskos, Nikos ^a   Praktiknjo, Samantha D ^a   Grosswendt, Stefanie ^a   Wahle, Philipp ^a   Ruffault, Pierre Louis ^a   Ayoub, Salah ^a   Schreyer, Luisa ^a   Boltengagen, Anastasiya ^a   Birchmeier, Carmen ^a   Zinzen, Robert ^a   Kocks, Christine ^a   Rajewsky, Nikolaus ^a  

^a MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

Author keywords

Alcohol based fixation; Drop seq; Droplet based single cell transcriptomics; Fixation; Fluorescent activated cell sorting (FACS); Gene expression profiling; Methanol; Primary cells; Tissue

Indexed keywords

MESSENGER RNA; METHANOL;

ANIMAL; CELL CULTURE; CEREBELLUM; CHEMISTRY; CYTOLOGY; DEVICES; DROSOPHILA; FLOW CYTOMETRY; GENE EXPRESSION PROFILING; HUMAN; MOUSE; NONMAMMALIAN EMBRYO; PROCEDURES; RHOMBENCEPHALON; SEQUENCE ANALYSIS; SINGLE CELL ANALYSIS; SOFTWARE;

ANIMALS; CELLS, CULTURED; CEREBELLUM; DROSOPHILA; EMBRYO, NONMAMMALIAN; FLOW CYTOMETRY; GENE EXPRESSION PROFILING; HUMANS; METHANOL; MICE; RHOMBENCEPHALON; RNA, MESSENGER; SEQUENCE ANALYSIS, RNA; SINGLE-CELL ANALYSIS; SOFTWARE;

EID: 85019374719     PISSN: None     EISSN: 17417007     Source Type: Journal    
DOI: 10.1186/s12915-017-0383-5     Document Type: Article

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