Oocyte, embryo and blastocyst cryopreservation in art: Systematic review and meta-Analysis comparing slow-freezing versus vitrification to produce evidence for the development of global guidance

Human Reproduction Update

Volumn 23, Issue 2, 2017, Pages 139-155

  Rienzi, Laura ^a   Gracia, Clarisa ^b   Maggiulli, Roberta ^a   Labarbera, Andrew R ^c   Kaser, Daniel J ^d   Ubaldi, Filippo M ^a   Vanderpoel, Sheryl ^e,f   Racowsky, Catherine ^d  

^a GENERA Centre for Reproductive Medicine   (Italy)

^b UNIVERSITY OF PENNSYLVANIA   (United States)

^c AMERICAN SOCIETY FOR REPRODUCTIVE MEDICINE   (United States)

^d HARVARD MEDICAL SCHOOL   (United States)

^e WORLD BANK   (United States)

^f POPULATION COUNCIL   (United States)

Author keywords

Blastocyst; Cryopreservation; Embryo; Oocyte; Slow freezing; Vitrification; World health organization

Indexed keywords

ARTICLE; BLASTOCYST; CLINICAL OUTCOME; COHORT ANALYSIS; CONTROLLED STUDY; CRYOPRESERVATION; EMBRYO; FEMALE; HUMAN; HUMAN EXPERIMENT; LIVE BIRTH; MEDLINE; META ANALYSIS; OBSTETRIC DELIVERY; OOCYTE; PREGNANCY RATE; RANDOMIZED CONTROLLED TRIAL (TOPIC); RISK FACTOR; SECONDARY ANALYSIS; STATISTICAL SIGNIFICANCE; SYSTEMATIC REVIEW; THAWING; VITRIFICATION; WARMING; WORLD HEALTH ORGANIZATION; BIRTH RATE; EMBRYO CULTURE; EMBRYO TRANSFER; ODDS RATIO; PREGNANCY; STANDARDS;

BIRTH RATE; BLASTOCYST; COHORT STUDIES; CRYOPRESERVATION; EMBRYO CULTURE TECHNIQUES; EMBRYO TRANSFER; FEMALE; HUMANS; ODDS RATIO; OOCYTES; PREGNANCY; PREGNANCY RATE; RANDOMIZED CONTROLLED TRIALS AS TOPIC;

EID: 85019329790     PISSN: 13554786     EISSN: 14602369     Source Type: Journal    
DOI: 10.1093/humupd/dmw038     Document Type: Article

References (139)

1. AbdelHafez FF, Desai N, Abou-Setta AM, Falcone T, Goldfarb J. et al. Slow-freezing, vitrification and ultra-rapid freezing of human embryos: A systematic review and meta-Analysis. Reprod Biomed Online 2010;20:209-222.
2. Abdelhafez F, Xu J, Goldberg J, Desai N. Vitrification in open and closed carriers at dif ferent cell stages: Assessment of embryo survival, development, dna integrity and stability during vapor phase storage for transport. BMC Biotechnol 2011;11:29.
3. Almodin CG, Minguetti-Camara VC, Paixao CL, Pereira PC. Embryo development and gestation using fresh and vitrified oocytes. Hum Reprod 2010;25:1192-1198.
4. Antinori M, Licata E, Dani G, Cerusico F, Versaci C, Antinori S. Cryotop vitrifica tion of human oocytes results in high survival rate and healthy deliveries. Reprod Biomed Online 2007;14:72-79.
5. Argyle CE, Harper JC, Davies MC. Oocyte cryopreservation: where are we now? Hum Reprod Update 2016;22:440-449.
6. Balaban B, Urman B, Ata B, Isiklar A, Larman MG, Hamilton R, Gardner DK. A ran domized controlled study of human Day 3 embryo cryopreservation by slow freezing or vitrification: vitrification is associated with higher survival, metabolism and blastocyst formation. Hum Reprod 2008;23:1976-1982.
7. Belva F, Bonduelle M, Roelants M, Verheyen G, Van Landuyt L. Neonatal health including congenital malformation risk of 1072 children born after vitrified embryo transfer. Hum Reprod 2016;31:1610-1620.
8. Bernal D, Colturato LF, Leef DM, Kort HI, Nagy ZP. Evaluation of blastocyst recu peration, implantation and pregnancy rates after vitrification/warming or slow freezing/ thawing cycles. Fertil Steril 2008;90:277-278.
9. Borini A, Lagalla C, Bonu MA, Bianchi V, Flamigni C, Coticchio G. Cumulative preg nancy rates resulting from the use of fresh and frozen oocytes: 7 years experi ence. Reprod Biomed Online 2006a;12:481-486.
10. Borini A, Sciajno R, Bianchi V, Sereni E, Flamigni C, Coticchio G. Cinical outcome of oocyte cryopreservation after slow-cooling with a protocol utilizing a high sucrose concentration. Hum Reprod 2006b;21:512-517.
11. Borini A, Bianchi V, Bonu MA, Sciajno R, Sereni E, Cattoli M, Mazzone S, Trevisi MR, Iadarola I, Distratis V et al. Evidence-based clinical outcome of oocyte slow cooling. Reprod Biomed Online 2007;15:175-181.
12. Borini A, Levi Setti PE, Anserini P, De Luca R, De Santis L, Porcu E, La Sala GB, Ferraretti A, Bartolotti T, Coticchio G et al. Multicenter observational study on slow-cooling oocyte cryopreservation: clinical outcome. Fertil Steril 2010;94: 1662-1668.
13. Cao YX, Xing Q, Li L, Cong L, Zhang ZG, Wei ZL, Zhou P. Comparison of sur vival and embryonic development in human oocytes cryopreserved by slow freezing and vitrification. Fertil Steril 2009;92:1306-1311.
14. CDC, Centres for Disease Control and Prevention. Reproductive Health. Assisted Reproductive Technology. National Summary and Fertility Clinic Reports 2011. http://www.cdc.gov/ART/ART2011.
15. Chamayou S, Alecci C, Ragolia C, Storaci G, Maglia E, Russo E, Guglielmino A. Comparison of in-vitro outcomes from cryopreserved oocytes and sibling fresh oocytes. Reprod Biomed Online 2006;12:730-736.
16. Chian RC, Huang JY, Gilber L, Son WY, Holzer H, Cui SJ, Buckett WM, Tulandi T, Tan SL. Obstetric outcomes following vitrification of in vitro and in vivo matured oocytes. Fertil Steril 2009;91:2391-2398.
17. Cil AP, Bang H, Oktay K. Age-specific probability of live birth with oocyte cryo preservation: An individual patient data meta-Analysis. Fertil Steril 2013;100:492-499.
18. Clua E, Tur R, Coroleu B, Boada M, Rodríguez I, Barri PN, Veiga A. Elective single embryo transfer in oocyte donation programmes: should it be the rule? Reprod Biomed Online 2012;25:642-648.
19. Cobo A, Kuwayama M, Pérez S, Ruiz A, Pellicer A, Remohí J. Comparison of con comitant outcome achieved with fresh and cryopreserved donor oocytes vitri fied by the Cryotop method. Fertil Steril 2008;89:1657-1664.
20. Cobo A, Meseguer M, Remohi J, Pellicer A. Use of cryo-banked oocytes in an ovum donation programme: A prospective, randomized, controlled, clinical trial. Hum Reprod 2010;25:2239-2246.
21. Cobo A, Remohí J, Chang CC, Nagy ZP. Oocyte cryopreservation for donor egg banking. Reprod Biomed Online 2011a;23:341-346.
22. Cobo A, Diaz C. Clinical application of oocyte vitrification: A systematic review and meta-Analysis of randomized controlled trials. Fertil Steril 2011b;96:277-285.
23. Cobo A, Garrido N, Crespo J, José R, Pellicer A. Accumulation of oocytes: A new strategy for managing low-responder patients. Reprod Biomed Online 2012;24: 424-432.
24. Cobo A, Serra V, Garrido N, Olmo L, Pellicer A, Remohí J. Obstetric and peri natal outcome of babies born from vitrified oocytes. Fertil Steril 2014;102: 1006-1015.
25. Cohen J, Simons RF, Edwards RG, Fehilly CB, Fishel SB. Pregnancies following the frozen storage of expanding human blastocysts. J In Vitro Fert Embryo Transf 1985; 2:59-64.
26. Coetsier T, Dhont M. Avoiding multiple pregnancies in in-vitro fertilization: who s afraid of single embryo transfer? Hum Reprod 1998;13:2663-2664.
27. Debrock S, Peeraer K, Fernandez Gallardo E, De Neubourg D, Spiessens C, D Hooghe TM. Vitrification of cleavage stage day 3 embryos results in higher live birth rates than conventional slow-freezing: A RCT. Hum Reprod 2015;30:1820-1830.
28. De Munck N, Santos-Ribeiro S, Stoop D, Van de Velde H, Verheyen G. Open ver sus closed oocyte vitrification in an oocyte donation program: A prospective ran domized sibling oocyte study. Hum Reprod 2016;31:337-384.
29. De Santis L, Cino I, Rabellotti E, Papaleo E, Calzi F, Fusi FM, Brigante C, Ferrari A. Oocyte cryopreservation: clinical outcome of slow-cooling protocols differing in sucrose concentration. Reprod Biomed Online 2007;14:57-63.
30. De Vos M, Smitz J, Woodruff TK. Fertility preservation in women with cancer. Lancet 2014;384:1302-1310.
31. Devroey P, Polyzos NP, Blockeel C. An OHSS-Free Clinic by segmentation of IVF treatment. Hum Reprod 2011;26:2593-2597.
32. Doyle JO, Richter KS, Lim J, Stillman RJ, Graham JR, Tucker MJ. Successful elective and medically indicated oocyte vitrification and warming for autologous in vitro fertilization, with predicted birth probabilities for fertility preservation according to number of cryopreserved oocytes and age at retrieval. Fertil Steril 2016;105: 459-466.
33. Edgar DH, Gook DA. A critical appraisal of cryopreservation (slow cooling versus vitrification) of human oocytes and embryos. Hum Reprod Update 2012;18:536-554.
34. Escribá MJ, Zulategui JF, Galán A, Mercader A, Remohí J. de los Santos MJ. Vitrification of preimplantation genetically diagnosed human blastocysts and its contribution to the cumulative ongoing pregnancy rate per cycle by using a closed device. Fertil Steril 2008;89:840-846.
35. Ethics Committee of American Society for Reproductive Medicine. Fertility preser vation and reproduction in patients facing gonadotoxic therapies: A committee opinion. Fertil Steril 2013;100:1224-1231.
36. Evans J, Hannan NJ, Edgell TA, Vollenhoven BJ, Lutjen PJ, Osianlis T, Salamonsen LA, Rombauts L. Fresh versus frozen embryo transfer: backing clinical decisions with scientific and clinical evidence. Hum Reprod Update 2014;20:808-821.
37. Fabbri R, Porcu E, Marsella T, Rocchetta G, Venturoli S, Flamigni C. Human oocyte cryopreservation: new perspectives regarding oocyte survival. Hum Reprod 2001; 16:411-416.
38. Fadini R, Brambillasca F, Renzini MM, Merola M, Comi R, De Ponti E, Dal Canto MB. Human oocyte cryopreservation: comparison between slow and ultrarapid methods. Reprod Biomed Online 2009;19:171-180.
39. Fasano G, Fontenelle N, Vannin AS, Biramane J, Devreker F, Englert Y, Delbaere A. A randomized controlled trial comparing two vitrification methods versus slow-freezing for cryopreservation of human cleavage stage embryos. J Assist Reprod Genet 2014;31:241-247.
40. Dahdouh EM, Balayla J, García-Velasco JA. Impact of blastocyst biopsy and compre hensive chromosome screening technology on preimplantation genetic screening: A systematic review of randomized controlled trials. Reprod Biomed Online 2015;30:281-289.
41. ESHRE Campus Course Report. Prevention of twin pregnancies after IVF/ICSI by single embryo transfer. Hum Reprod 2001;4:790-800.
42. Fatemi HM, Kyrou D, Bourgain C, Van den Abbeel E, Griesinger G, Devroey P. Cryopreserved-Thawed human embryo transfer: spontaneous natural cycle is superior to human chorionic gonadotropin-induced natural cycle. Fertil Steril 2010;94:2054-2058.
43. Forman EJ, Li X, Ferry KM, Scott K, Treff NR, Scott RT Jr. Oocyte vitrification does not increase the risk of embryonic aneuploidy or diminish the implantation potential of blastocysts created after intracytoplasmic sperm injection: A novel, paired randomized controlled trial using DNA fingerprinting. Fertil Steril 2012;3: 644-649.
44. Gardner DK, Meseguer M, Rubio C, Treff NR. Diagnosis of human preimplantation embryo viability. Hum Reprod Update 2015;21:727-747.
45. García JI, Noriega-Portella L, Noriega-Hoces L. Efficacy of oocyte vitrification com bined with blastocyst stage transfer in an egg donation program. Hum Reprod 2011;26:782-790.
46. Glujovsky D, Riestra B, Sueldo C, Fiszbajn G, Repping S, Nodar F, Papier S, Ciapponi A. Vitrification versus slow-freezing for women undergoing oocyte cryopreservation. Cochrane Database Syst Rev 2014;5:CD010047.
47. Griesinger G, von Otte S, Schroer A, Ludwig AK, Diedrich K, Al-Hasani S, Schultze-Mosgau A. Elective cryopreservation of all pronuclear oocytes after GnRH agonist triggering of final oocyte maturation in patients at risk of develop ing OHSS: A prospective, observational proof-of-concept study. Hum Reprod 2007;22:1348-1352.
48. Griesinger G, Schultz L, Bauer T et al. Ovarian hyperstimulation syndrome preven tion by gonadotropin-releasing hormone agonist triggering of final oocyte matur ation in a gonadotropin-releasing hormone antagonist protocol in combination with a freeze-All strategy: A prospective multicentric study. Fertil Steril 2011;95: 2029-2033.
49. Grifo JA, Noyes N. Delivery rate using cryopreserved oocytes is comparable to conventional in vitro fertilization using fresh oocytes: potential fertility preserva tion for female cancer patients. Fertil Steril 2010;93:391-396.
50. Guerif F, Le Gouge A, Giraudeau B, Poindron J, Bidault R, Gasnier O, Royere D. Limited value of morphological assessment at days 1 and 2 to predict blastocyst development potential: A prospective study based on 4042 embryos. Hum Reprod 2007;2:1973-1981.
51. Heng BC. Oocyte cryopreservation as alternative to embryo cryopreservation-some pertinent ethical concerns. Reprod Biomed Online 2007;14:402-403.
52. Herrero L, Pareja S, Losada C, Cobo AC, Pellicer A, Garcia-Velasco JA. Avoiding the use of human chorionic gonadotropin combined with oocyte vitrification and GnRH agonist triggering versus coasting: A new strategy to avoid ovarian hyper stimulation syndrome. Fertil Steril 2011;95:1137-1140.
53. Huang CC, Lee TH, Chen SU et al. Successful pregnancy following blastocyst cryo preservation using super-cooling ultra-rapid vitrification. Hum Reprod 2005;20: 122-128.
54. Isachenko V, Montag M, Isachenko E, Dessole S, Nawroth F, van der Ven H. Aseptic vitrification of human germinal vesicle oocytes using dimethyl sulfoxide as a cryoprotectant. Fertil Steril 2006;85:741-747.
55. Jelinkova L, Selman HA, Arav A, Strehler E, Reeka N, Sterzik K. Twin pregnancy after vitrification of 2-pronuclei human embryos. Fertil Steril 2002;77:412-414.
56. Jericho H, Wilton L, Gook DA, Edgar DH. A modified cryopreservation method increases the survival of human biopsied cleavage stage embryos. Hum Reprod 2003;18:568-571.
57. Jin B, Mazur P. High survival of mouse oocytes/embryos after vitrification without permeating cryoprotectants followed by ultra-rapid warming with an IR laser pulse. Sci Rep 2015;5:927.
58. Kaser DJ, Racowsky C. Clinical outcomes following selection of human preimplan tation embryos with time-lapse monitoring: A systematic review. Hum Reprod Update 2014;20:617-631.
59. Kaartinen N, Kananen K, Huhtala H, Keränen S, Tinkanen H. The freezing method of cleavage stage embryos has no impact on the weight of the newborns. J Assist Reprod Genet 2016;33:393-399.
60. Kim S, Lee S, Lee J et al. Study on the vitrification of human blastocysts. II: Effect of vitrification on the implantation and the pregnancy of human blastocysts. Korean J Fertil Steril 2000;27:67-74.
61. Kolibianakis EM, Venetis CA, Tarlatzis BC. Cryopreservation of human embryos by vitrification or slow-freezing: which one is better? Curr Opin Obstet Gynecol 2009; 21:270-274.
62. Kuleshova L, Gianaroli L, Magli C, Ferraretti A, Trounson A. Birth following vitrifica tion of a small number of human oocytes: case report. Hum Reprod 1999;14: 3077-3079.
63. Kuleshova LL, Shaw JM. A strategy for rapid cooling of mouse embryos within a double straw to eliminate the risk of contamination during storage in liquid nitro gen. Hum Reprod 2000;15:2604-2609.
64. Kupka MS, D Hooghe T, Ferraretti AP, de Mouzon J, Erb K, Castilla JA, Calhaz Jorge C, De Geyter CH, Goossens V. Assisted reproductive technology in Europe, 2011: results generated from European registers by ESHRE. Hum Reprod 2016;31:233-248.
65. Kuwayama M, Vajta G, Kato O, Leibo SP. Highly efficient vitrification method for cryopreservation of human oocytes. Reprod Biomed Online 2005a;11: 300-308.
66. Kuwayama M, Vajta G, Ieda S et al. Comparison of open and closed methods for vitrification of human embryos and the elimination of potential contamination. Reprod Biomed Online 2005b;11:608-614.
67. La Sala GB, Nicoli A, Villani MT, Pescarini M, Gallinelli A, Blickstein I. Outcome of 518 salvage oocyte-cryopreservation cycles performed as a routine procedure in an in vitro fertilization program. Fertil Steril 2006;86:1423-1427.
68. Lassalle B, Testart J, Renard JP. Human embryo features that influence the suc cess of cryopreservation with the use of 1,2 propanediol. Fertil Steril 1985;44: 645-651.
69. Lee E, Illingworth P, Wilton L, Chambers GM. The clinical effectiveness of preim plantation genetic diagnosis for aneuploidy in all 24 chromosomes (PGD-A): sys tematic review. Hum Reprod 2015;30:473-483.
70. Leibo SP, Songsasen N. Cryopreservation of gametes and embryos of non domestic species. Theriogenology 2002;57:303-326.
71. Levi Setti PE, Albani E, Novara PV, Cesana A, Morreale G. Cryopreservation of supernumerary oocytes in IVF/ICSI cycles. Hum Reprod 2006;21:370-375.
72. Levi Setti PE, Albani E, Morenghi E, Morreale G, Delle Piane L, Scaravelli G, Patrizio P. Comparative analysis of fetal and neonatal outcomes of pregnancies from fresh and cryopreserved/thawed oocytes in the same group of patients. Fertil Steril 2013;100:396-401.
73. Levi Setti PE, Porcu E, Patrizio P, Vigiliano V, de Luca R, d Aloja P, Spoletini R, Scaravelli G. Human oocyte cryopreservation with slow-freezing versus vitrifica tion. Results from the National Italian Registry Data, 2007-2011. Fertil Steril 2014;102:90-95.
74. Levi-Setti PE, Borini A, Patrizio P, Bolli S, Vigiliano V, De Luca R, Scaravelli G. ART results with frozen oocytes: data from the Italian ART registry (2005-2013). J Assist Reprod Genet 2016;33:123-128.
75. Li Y, Chen ZJ, Yang HJ et al. Comparison of vitrification and slow-freezing of human day 3 cleavage stage embryos: post vitrification development and pregnancy out comes. Zhonghua Fu Chan Ke Za Zhi 2007;42:753-755.
76. Li Z, Wang YA, Ledger W, Edgar DH, Sullivan EA. Clinical outcomes following cryopreservation of blastocysts by vitrification or slow-freezing: A population based cohort study. Hum Reprod 2014;29:2794-2801.
77. Li Z, Sullivan EA, Chapman M, Farquhar C, Wang YA. Risk of ectopic pregnancy lowest with transfer of single frozen blastocyst. Hum Reprod 2015;30: 2048-2054.
78. Liebermann J, Tucker MJ. Comparison of vitrification and conventional cryopreser vation of day 5 and day 6 blastocysts during clinical application. Fertil Steril 2006; 86:20-26.
79. Liu SY, Teng B, Fu J, Li X, Zheng Y, Sun XX. Obstetric and neonatal outcomes after transfer of vitrifiedearlycleavageembryos. Hum Reprod 2013;28:2093-2100.
80. Loutradi KE, Kolibianakis EM, Venetis CA, Papanikolaou EG, Pados G, Bontis I, Tarlatzis BC. Cryopreservation of human embryos by vitrification or slow-freez ing: A systematic review and meta-Analysis. Fertil Steril 2008;90:186-193.
81. Maheshwari A, Pandey S, Shetty A, Hamilton M, Bhattacharya S. Obstetric and perinatal outcomes in singleton pregnancies resulting from the transfer of frozen thawed versus fresh embryos generated through in vitro fertilization treatment: A systematic review and meta-Analysis. Fertil Steril 2012;98:368-337.
82. Macaldowie A, Wang YA, Chambers GM, Sullivan EA. Assisted Reproductive Technology in Australia and New Zealand 2010. Sydney, NSW: Australian Institute of Health and Welfare, 2012.
83. Martinez M, Rabadan S, Domingo J, Cobo A, Pellicer A, Garcia-Velasco JA. Obstetric outcome after oocyte vitrification and warming for fertility preserva tion in women with cancer. Reprod Biomed Online 2014;29:722-728.
84. Mazur P. Equilibrium, quasi-equilibrium, and nonequilibrium freezing of mammalian embryos. Cell Biophys 1990;17:53-92.
85. Mukaida T, Wada S, Takahashi K, Pedro PB, An TZ, Kasai M. Vitrification of human embryos based on the assessment of suitable conditions for 8-cell mouse embryos. Hum Reprod 1998;13:2874-2879.
86. Nachtigall RD, Mac Dougall K, Lee M, Harrington J, Becker G. What do patients want? Expectations and perceptions of IVF clinic information and support regard ing frozen embryo disposition. Fertil Steril 2010;94:2069-2072.
87. Nagy ZP, Chang CC, Shapiro DB, Bernal DP, Elsner CW, Mitchell-Leef D, Toledo AA, Kort HI. Clinical evaluation of the efficiency of an oocyte donation program using egg cryo-banking. Fertil Steril 2009;92:520-526.
88. Nagy ZP, Cobo A, Chang C. Oocyte Vitrification: donor egg banking in Vitrification in Assisted Reproduction, 2nd edn. CRC Press, 2015;129-136.
89. Noyes N, Porcu E, Borini A. Over 900 oocyte cryopreservation babies born with no apparent increase in congenital anomalies. Reprod Biomed Online 2009;18: 769-776.
90. Oktay K, Cil AP, Bang H. Efficiency of oocyte cryopreservation: A meta-Analysis. Fertil Steril 2006;86:70-80.
91. Paffoni A, Alagna F, Somigliana E, Restelli L, Brevini TA, Gandolfi F, Ragni G. Developmental potential of human oocytes after slow freezing or vitrification: A randomized in vitro study based on parthenogenesis. Reprod Sci 2008;15:1027-1033.
92. Parmegiani L, Cognigni GE, Filicori M. Ultra-violet sterilization of liquid nitrogen prior to vitrification. Hum Reprod 2009;24:2969.
93. Parmegiani L, Cognigni GE, Bernardi S, Cuomo S, Ciampaglia W, Infante FE, Tabarelli de Fatis C, Arnone A, Maccarini AM, Filicori M. Efficiency of aseptic open vitrification and hermetical cryostorage of human oocytes. Reprod Biomed Online 2011;23:505-512.
94. Pelkonen S, Koivunen R, Gissler M, Nuojua-Huttunen S, Suikkari AM, Hydén Granskog C, Martikainen H, Tiitinen A, Hartikainen AL. Perinatal outcome of children born after frozen and fresh embryo transfer: The Finnish cohort study 1995-2006. Hum Reprod 2010;25:914-923.
95. Pinborg A, Loft A, Aaris Henningsen AK, Rasmussen S, Andersen AN. Infant out come of 957 singletons born after frozen embryo replacement: The Danish National Cohort Study 1995-2006. Fertil Steril 2010;94:1320-1327.
96. Potdar N, Gelbaya TA, Nardo LG. Oocyte vitrification in the 21st century and post-warming fertility outcomes: A systematic review and meta-Analysis. Reprod Biomed Online 2014;29:159-176.
97. Racowsky C, Ohno-Machado L, Kim J, Biggers JD. Is there an advantage in scoring early embryos on more than one day? Hum Reprod 2009;24:2104-2113.
98. Rama Raju GA, Haranath GB, Krishna KM et al. Vitrification of human 8-cell embryos, a modified protocol for better pregnancy rates. Reprod Biomed Online 2005;11:434-437.
99. Rezazadeh Valojerdi M, Eftekhari-Yazdi P, Karimian L et al. Vitrification versus slow freezing gives excellent survival post warming embryo morphology and pregnancy outcomes for human cleavedembryos. J Assist Reprod Genet 2009;26:347-354.
100. Rienzi L, Romano S, Albricci L, Maggiulli R, Capalbo A, Baroni E, Colamaria S, Sapienza F, Ubaldi F. Embryo development of fresh versus vitrified metaphase II oocytes after ICSI: A prospective randomized sibling-oocyte study. Hum Reprod 2010;25:66-73.
101. Rienzi L, Ubaldi FM. Oocyte versus embryo cryopreservation for fertility preserva tion in cancer patients: guaranteeing a women s autonomy. J Assist Reprod Genet 2015;32:1195-1196.
102. Roy TK, Brandi S, Tappe NM, Bradley CK, Vom E, Henderson C, Lewis C, Battista K, Hobbs B, Hobbs S et al. Embryo vitrification using a novel semi-Automated closed system yields in vitro outcomes equivalent to the manual Cryotop meth od. Hum Reprod 2014;29:2431-2438.
103. Roque M, Lattes K, Serra S, Sola I, Geber S, Carreras R, Checa MA. Fresh embryo transfer versus frozen embryo transfer in in vitro fertilization cycles: A systematic review and meta-Analysis. Fertil Steril 2013;99:156-162.
104. Schoolcraft WB, Treff NR, Stevens JM, Ferry K, Katz-Jaffe M, Scott RT Jr. Live birth outcome with trophectoderm biopsy, blastocyst vitrification, and single nucleotide polymorphism microarray-based comprehensive chromosome screening in infertile patients. Fertil Steril 2011;96:638-640.
105. Selman HA, El-Danasouri I. Pregnancies derived from vitrified human zygotes. Fertil Steril 2002;77:422-423.
106. Shapiro BS, Daneshmand ST, Garner FC, Aguirre M, Hudson C. Freeze-All at the blastocyst or bipronuclear stage: A randomized clinical trial. Fertil Steril 2015;104: 1138-1144.
107. Siano L, Engmann L, Nulsen J, Benadiva C. A prospective pilot study comparing fer tilization and embryo development between fresh and vitrified sibling oocytes. Conn Med 2013;77:211-217.
108. Sifer C, Sermondade N, Dupont C, Poncelet C, Cédrin-Durnerin I, Hugues JN, Benzacken B, Levy R. [Outcome of embryo vitrification compared to slow freezing process at earlycleavage stages. Report of the first French birth]. Gynecol Obstet Fertil 2012;40:158-161.
109. Smith GD, Serafini PC, Fioravanti J, Yadid I, Coslovsky M, Hassun P, Alegretti JR, Motta EL. Prospective randomized comparison of human oocyte cryopreserva tion with slow-rate freezing or vitrification. Fertil Steril 2010;94:2088-2095.
110. Sole M, Santalo J, Boada M, Clua E, Rodriguez I, Martinez F, Coroleu B, Barri PN, Veiga A. How does vitrification affect oocyte viability in oocyte donation cycles? A prospective study to compare outcomes achieved with fresh versus vitrified sibling oocytes. Hum Reprod 2013;28:2087-2092.
111. Stehlik E, Stehlik J, Katayama KP, Kuwayama M, Jambor V, Brohammer R, Kato O. Vitrification demonstrates significant improvement versus slow-freezing of human blastocysts. Reprod Biomed Online 2005;11:53-57.
112. Stoop D, Nekkebroeck J, Devroey P. A survey on the intentions and attitudes towards oocyte cryopreservation for non-medical reasons among women of reproductive age. Hum Reprod 2011;3:655-661.
113. Stoop D, Cobo A, Silber S. Fertility preservation for age-related fertility decline. Lancet 2014;384:1311-1319.
114. Summers D, Check JH, Choe JK. A prospective comparison of outcome following cryopreservation using vitrification versus a modified slow-freeze protocol of 2 pronuclear (2PN) and day 3 multi-cell embryos. Clin Exp Obstet Gynecol 2016; 43:330-331.
115. Testart J, Lassalle B, Belaisch-Allart J, Hazout A, Forman R, Rainhorn JD, Frydman R. High pregnancy rate after early human embryo freezing. Fertil Steril 1986;46:268-272.
116. Tiitinen A, Halttunen M, Härkki P, Vuoristo P, Hyden-Granskog C. Elective single embryo transfer: The value of cryopreservation. Hum Reprod 2001;6:1140-1144.
117. Trokoudes KM, Pavlides C, Zhang X. Comparison outcome of fresh and vitrified donor oocytes in an egg-sharing donation program. Fertil Steril 2011;95:1996-2000.
118. Trounson A, Mohr L. Human pregnancy following cryopreservation, thawing and transfer of an eight-cell embryo. Nature 1983;305:707-709.
119. Ubaldi F, Anniballo R, Romano S, Baroni E, Albricci L, Colamaria S, Capalbo A, Sapienza F, Vajta G, Rienzi L. Cumulative ongoing pregnancy rate achieved with oocyte vitrification and cleavage stage transfer without embryo selection in a standard infertility program. Hum Reprod 2010;25:1199-1205.
120. Ubaldi FM, Capalbo A, Colamaria S, Ferrero S, Maggiulli R, Vajta G, Sapienza F, Cimadomo D, Giuliani M, Gravotta E et al. Reduction of multiple pregnancies in the advanced maternal age population after implementation of an elective single embryo transfer policy coupled with enhanced embryo selection: pre-and post intervention study. Hum Reprod 2015;30:2097-2106.
121. Vajta G, Holm P, Kuwayama M, Booth PJ, Jacobsen H, Greve T, Callesen H. Open Pulled Straw (OPS) vitrification: A new way to reduce cryoinjuries of bovine ova and embryos. Mol Reprod Dev 1998;51:53-58.
122. Vajta G, Nagy ZP. Are programmable freezers still needed in the embryo labora tory? Review on vitrification. Reprod Biomed Online 2006;12:779-796.
123. Vajta G, Rienzi L, Ubaldi FM. Open versus closed systems for vitrification of human oocytes and embryos. Reprod Biomed Online 2015;30:325-333.
124. Van Landuyt L, Van de Velde H, De Vos A, Haentjens P, Blockeel C, Tournaye H, Verheyen G. Influence of cell loss after vitrification or slow-freezing on further in vitro development and implantation of human Day 3 embryos. Hum Reprod 2013;28:2943-2949.
125. Vanderzwalmen P, Ectors F, Grobet L, Prapas Y, Panagiotidis Y, Vanderzwalmen S, Stecher A, Frias P, Liebermann J, Zech NH. Aseptic vitrification of blastocysts from infertile patients, egg donors and after IVM. Reprod Biomed Online 2009;19: 700-707.
126. Veeck LL, Bodine R, Clarke RN, Berrios R, Libraro J, Moschini RM, Zaninovic N, Rosenwaks Z. High pregnancy rates can be achieved after freezing and thawing human blastocysts. Fertil Steril 2004;82:1418-1427.
127. Virant-Klun I, Bacer-Kermavner L, Tomazevic T, Vrtacnik-Bokal E. Slow oocyte freezing and thawing in couples with no sperm or an insufficient number of sperm on the day of in vitro fertilization. Reprod Biol Endocrinol 2011;9:19.
128. Wang YA, Sullivan EA, Black D, Dean J, Bryant J, Chapman M. Preterm birth and low birth weight after assisted reproductive technology-related pregnancy in Australia between 1996 and 2000. Fertil Steril 2005;83:1650-1165.
129. Wang XL, Zhang X, Qin YQ, Hao DY, Shi HR. Outcomes of day 3 embryo trans fer with vitrification using Cryoleaf: A 3-year follow-up study. J Assist Reprod Genet 2012;29:883-889.
130. Wennerholm UB, Söderström-Anttila V, Bergh C, Aittomäki K, Hazekamp J, Nygren KG, Selbing A, Loft A. Children born after cryopreservation of embryos or oocytes: A systematic review of outcome data. Hum Reprod 2009;24:2158-2172.
131. Whittingham DG, Leibo SP, Mazur P. Survival of mouse embryos frozen to -196 degrees and -269 degrees C. Science 1972;178:411-414.
132. WHO Handbook for Guideline Development, 2nd edn. Geneva: WHO Press, World Health Organization, 2012. Available: http://apps.who.int/iris/bitstream/ 10665/75146/1/9789241548441_eng.pdf.
133. Wilding MG, Capobianco C, Montanaro N, Kabili G, Di Matteo L, Fusco E, Dale B. Human cleavage-stage embryo vitrification is comparable to slow-rate cryo preservation in cycles of assisted reproduction. J Assist Reprod Genet 2010;27: 549-554.
134. Willadsen SM Factors affecting the survival of sheep embryos during deep freezing and thawing. In: Elliott K, Whelan L (eds). The Freezing of Mammalian Embryos, Ciba Foundation Symposium 52. Amsterdam: Elsevier, 1977;175-194.
135. Youssef MA, Van der Veen F, Al-Inany HG, Mochtar MH, Griesinger G, Nagi Mohesen M, Aboulfoutouh I, van Wely M. Gonadotropin-releasing hormone agonist versus HCG for oocyte triggering in antagonist-Assisted reproductive technology. Cochrane Database Syst Rev 2014;10:CD008046.
136. Zhang X, Trokoudes KM, Pavlides C. Vitrification of biopsied embryos at cleavage, morula and blastocyst stage. Reprod Biomed Online 2009;19:526-531.
137. Zheng WT, Zhuang GL, Zhou CQ et al. Comparison of the survival of human biopsied embryos after cryopreservation with four different methods using non transferable embryos. Hum Reprod 2005;20:1615-1618.
138. Zhu HY, Xue YM, Yang LY, Jiang LY, Ling C, Tong XM, Zhang SY. Slow freezing should not be totally substituted by vitrification when applied to day 3 embryo cryopreservation: An analysis of 5613 frozen cycles. J Assist Reprod Genet 2015; 32:1371-1377.
139. Zeilmaker GH, Alberda AT, van Gent I, Rijkmans CM, Drogendijk AC. Two preg nancies following transfer of intact frozen-Thawed embryos. Fertil Steril 1984;42: 293-296.