Enhanced limonene production by optimizing the expression of limonene biosynthesis and MEP pathway genes in E. Coli

Bioresources and Bioprocessing

Volumn 1, Issue 1, 2014, Pages

  Du, Fu Liang ^a   Yu, Hui Lei ^a   Xu, Jian He ^a   Li, Chun Xiu ^a  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Biosynthesis; Escherichia coli; Geranyl pyrophosphate synthase; Limonene; Limonene synthase; MEP pathway; Two phase culture system

Indexed keywords

BIOCHEMISTRY; BIOSYNTHESIS; FLAVOR COMPOUNDS; GENE EXPRESSION; MONOTERPENES;

CULTURE SYSTEMS; GERANYL PYROPHOSPHATE SYNTHASE; LIMONENE; LIMONENE SYNTHASE; MEP PATHWAYS; PHASE CULTURE; SYNTHASES; TWO PHASE; TWO-PHASE CULTURE SYSTEM;

ESCHERICHIA COLI;

EID: 85019294885     PISSN: None     EISSN: 21974365     Source Type: Journal    
DOI: 10.1186/s40643-014-0010-z     Document Type: Article

References (38)

1. Misawa N (2011) Pathway engineering for functional isoprenoids. Curr Opin Biotechnol 22:627–633
2. Colby SM, Alonso WR, Katahira EJ, Mcgarvey DJ, Croteau R (1993) 4S-Limonene synthase from the oil glands of spearmint (Mentha spicata): cDNA isolation, characterization, and bacterial expression of the catalytically active monoterpene cyclase. J Biol Chem 268:23016–23024
3. Alonso-Gutierrez J, Chan R, Batth TS, Adams PD, Keasling JD, Petzold CJ, Lee TS (2013) Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production. Metab Eng 19:33–41
4. Carter OA, Peters RJ, Croteau R (2003) Monoterpene biosynthesis pathway construction in Escherichia coli. Phytochemistry 64:425–433
5. Duetz WA, Bouwmeester H, Beilen JB, Witholt B (2003) Biotransformation of limonene by bacteria, fungi, yeasts, and plants. Appl Microbiol Biotechnol 61:269–277
6. Keasling JD (2010) Manufacturing molecules through metabolic engineering. Science 330:1355–1358
7. Tracy NI, ChenD CDW, Price GL (2009) Hydrogenated monoterpenes as diesel fuel additives. Fuel 88:2238–2240
8. Campos N, Rodriguez-Concepcion M, Sauret-Gueto S, Gallego F, Lois LM, Boronat A (2001) Escherichia coli engineered to synthesize isopentenyl diphosphate and dimethylallyl diphosphate from mevalonate: a novel system for the genetic analysis of the 2-c-methyl-d-erythritol-4-phosphate pathway for isoprenoid biosynthesis. Biochem J 353:59–67
9. Martin VJ, Pitera DJ, Withers ST, Newman JD, Keasling JD (2003) Engineering a mevalonate pathway in Escherichia coli for production of terpenoids. Nat Biotechnol 21:796–802
10. Pitera DJ, Paddon CJ, Newman JD, Keasling JD (2007) Balancing a heterologous mevalonate pathway for improved isoprenoid production in Escherichia coli. Metab Eng 9(2):193–207
11. Hunter WN (2007) The non-mevalonate pathway of isoprenoid precursor biosynthesis. J Biol Chem 282(30):21573–21577
12. Croteau RB, Davis EM, Ringer KL, Wildung MR (2005) (−)-Menthol biosynthesis and molecular genetics. Naturwissenschaften 92:562–577
13. Burke C, Croteau R (2002) Geranyl diphosphate synthase from Abies grandis: cDNA isolation, functional expression, and characterization. Arch Biochem Biophys 405:130–136
14. Ajikumar PK, Xiao WH, Tyo KEJ, Wang Y, Simeon F, Leonard E, Mucha O, Phon TH, Pfeifer B, Stephanopoulos G (2010) Isoprenoid pathway optimization for taxol precursor overproduction in Escherichia coli. Science 330:70–74
15. Leonarda E, Ajikumara PK, Thayer K, Xiao WH, Mo JD, Tidorb B, Stephanopoulosa G, Thayer K, Prather KLJ (2010) Combining metabolic and protein engineering of a terpenoid biosynthetic pathway for overproduction and selectivity control. Proc Natl Acad Sci 107:13654–13659
16. Xue J, Ahring BK (2011) Enhancing isoprene production by genetic modification of the 1-deoxy-d-xylulose-5-phosphate pathway in Bacillus subtilis. Appl Environ Microbiol 77:2399–2405
17. Zhao Y, Yang J, Qin B, Li Y, Sun Y, Su S, Xian M (2011) Biosynthesis of isoprene in Escherichia coli via methylerythritol phosphate (MEP) pathway. Appl Microbiol Biotechnol 90:1915–1922
18. Zurbriggen A, Kirst H, Melis A (2012) Isoprene production via the mevalonic acid pathway in Escherichia coli (bacteria). BioEnergy Research 5:814–828
19. Lv X, Xu H, Yu H (2013) Significantly enhanced production of isoprene by ordered coexpression of genes dxs, dxr, and idi in Escherichia coli. Appl Microbiol Biotechnol 97:2357–2365
20. Williams DC, McGarvey DJ, Katahira EJ, Croteau R (1998) Truncation of limonene synthase preprotein provides a fully active ‘pseudomature’ form of this monoterpene cyclase and reveals the function of the amino-terminal arginine pair. Biochemistry 37:12213–12220
21. Brosius J, Erfle M, Storella J (1985) Spacing of the −10 and −35 regions in the tac promoter: Effect on its in vivo activity. J Biol Chem 260:3539–3541
22. Brunner M, Bujard H (1987) Promoter recognition and promoter strength in the Escherichia coli system. EMBO J 6:3139–3144
23. Jang HJ, Yoon SH, Ryu HK, Kim JH, Wang CL, Kim JY, Oh DK, Kim SW (2011) Retinoid production using metabolically engineered Escherichia coli with a two-phase culture system. Microb Cell Fact 10:59
24. Zhou K, Zou R, Zhang C, Stephanopoulos G, Too HP (2013) Optimization of amorphadiene synthesis in Bacillus subtilis via transcriptional, translational, and media modulation. Biotechnol Bioeng 110:2556–2561
25. Song HB, Demain AL (1977) An improved colorimetric assay for polyols. Anal Biochem 81(1):18–20
26. Reiling KK, Yoshikuni Y, Martin VJ, Newman J, Bohlmann J, Keasling JD (2004) Mono and diterpene production in Escherichia coli. Biotechnol Bioeng 87:200–212
27. Lois LM, Rodriguez-Concepcion M, Gallego F, Campos N, Boronat A (2000) Carotenoid biosynthesis during tomato fruit development: regulatory role of 1-deoxy-d-xylulose-5-phosphate synthase. Plant J 22(6):503–513
28. Miller B, Heuser T, Zimmer W (2000) Functional involvement of a deoxy-xylulose-5-phosphate reductoisomerase gene harboring locus of Synechococcus leopoliensisin isoprenoid biosynthesis. FEBS Lett 481:221–226
29. Estevez JM, Cantero A, Reindl A, Reichler S, Leon P (2001) 1-Deoxy-d-xylulose-5-phosphate synthase, a limiting enzyme for plastidic isoprenoid biosynthesis in plants. J Biol Chem 276(25):22901–22909
30. Das A, Yoon SH, Lee SH, Kim JY, Oh DK, Kim SW (2007) An update on microbial carotenoid production: application of recent metabolic engineering tools. Appl Microbiol Biotechnol 77(3):505–512
31. Zaslaver A, Mayo AE, Rosenberg R, Bashkin P, Sberro H, Tsalyuk M, Surette MG, Alon U (2004) Just-in-time transcription program in metabolic pathways. Nat Genet 36:486–491
32. Nishizaki T, Tsuge K, Itaya M, Doi N, Yanagawa H (2007) Metabolic engineering of carotenoid biosynthesis in Escherichia coli by ordered gene assembly in Bacillus subtilis. Appl Environ Microbiol 73(4):1355–1361
33. Morrone D, Lowry L, Determan MK, Hershey DM, Xu M, Peters RJ (2010) Increasing diterpene yield with a modular metabolic engineering system in E. coli: comparison of MEV and MEP isoprenoid precursor pathway engineering. Appl Microbiol Biotechnol 85(6):1893–1906
34. Meng H, Wang Y, Hua Q, Zhang S, Wang X (2011) In silico analysis and experimental improvement of taxadiene heterologous biosynthesis in Escherichia coli. Biotechnol Bioprocess Eng 16(2):205–215
35. Yuan LZ, Rouvière PE, Larossa RA, Suh W (2006) Chromosomal promoter replacement of the isoprenoid pathway for enhancing carotenoid production in E. coli. Metab Eng 8(1):79–90
36. Kajiwara S, Fraser PD, Kondo K, Misawa N (1997) Expression of an exogenous isopentenyl diphosphate isomerase gene enhances isoprenoid biosynthesis in Escherichia coli. Biochem J 324:421–426
37. Ghimire GP, Lee HC, Sohng JK (2009) Improved Squalene Production via Modulation of the Methylerythritol 4-Phosphate Pathway and Heterologous Expression of Genes from Streptomyces peucetius ATCC 27952 Escherichia coli. Appl Environ Microbiol 75(22):7291–7293
38. Albrecht M, Misawa N, Sandmann G (1999) Metabolic engineering of the terpenoid biosynthetic pathway of Escherichia coli for production of the carotenoids β-carotene and zeaxanthin. Biotechnol Lett 21(9):791–795